Isolation and characterization of microorganisms from a landfarming of the province of san luis with potential capacity for use in bioaugmentation

Autores
Salinas, A.; Lijteroff, Rubén Enrique; Delfini, Claudio Daniel; Villegas, Liliana Beatriz
Año de publicación
2023
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The final disposal of industrial effluents with organic or inorganic compounds is a concern for companies and governments. Consequently, in recent decades, different methodologies have been used to reduce them, without causing damage to the environment, among which are bioremediation processes. Landfarming is an ex-situ bioremediation treatment process that is carried out in the upper zone of the soil. In San Luis Province there is a landfarming that receives effluents from chemical industry and has been used for more than 10 years. The aim of this work was to isolate microorganism from a landfarming soil with the capacity to tolerate the environmental stresses which can favor increasing and accelerating landfarming processes. The samples were collected in two stages, during the winter period of 2022: the first was taken from the supernatant of the effluent after its discharge; the second from the mud generated in the mixture of soil and effluent, after 5 days of discharge. The sampling points were 3 at different distances from the discharge of effluents. Two approaches were utilized to isolate microorganism from the landfarming soil: a direct isolation approach in EG medium following the streaked-on agar plate and enrichment approach in liquid medium incubated a 30°C, 150 rpm for 72 h. While, using a direct isolation approach only 12 morphotypes were isolated, using enrichment approach 15 morphotypes were isolated. To characterize the isolates, the characteristics of the colonies, the cells and the Gram coloration in the case of bacteria were taken into account. The isolates will be molecularly identified. It is well established that only approximately 1% of microorganisms on Earth can be readily cultivated in vitro. But this work aims to use microorganisms in bioaugmentation process, so it only focused on culturable microorganisms. These preliminary results show that landfarming has an active microbiota and it is feasible to isolate microorganisms that can be used in bioaugmentation processes with native strains
Fil: Salinas, A.. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Lijteroff, Rubén Enrique. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Delfini, Claudio Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Villegas, Liliana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
XL Reunión Científica Anual de la Sociedad de Biología de Cuyo
Mendoza
Argentina
Sociedad de Biología de Cuyo
Materia
LANDFARMING
MICROORGANISMS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/243033
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Isolation and characterization of microorganisms from a landfarming of the province of san luis with potential capacity for use in bioaugmentationSalinas, A.Lijteroff, Rubén EnriqueDelfini, Claudio DanielVillegas, Liliana BeatrizLANDFARMINGMICROORGANISMShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The final disposal of industrial effluents with organic or inorganic compounds is a concern for companies and governments. Consequently, in recent decades, different methodologies have been used to reduce them, without causing damage to the environment, among which are bioremediation processes. Landfarming is an ex-situ bioremediation treatment process that is carried out in the upper zone of the soil. In San Luis Province there is a landfarming that receives effluents from chemical industry and has been used for more than 10 years. The aim of this work was to isolate microorganism from a landfarming soil with the capacity to tolerate the environmental stresses which can favor increasing and accelerating landfarming processes. The samples were collected in two stages, during the winter period of 2022: the first was taken from the supernatant of the effluent after its discharge; the second from the mud generated in the mixture of soil and effluent, after 5 days of discharge. The sampling points were 3 at different distances from the discharge of effluents. Two approaches were utilized to isolate microorganism from the landfarming soil: a direct isolation approach in EG medium following the streaked-on agar plate and enrichment approach in liquid medium incubated a 30°C, 150 rpm for 72 h. While, using a direct isolation approach only 12 morphotypes were isolated, using enrichment approach 15 morphotypes were isolated. To characterize the isolates, the characteristics of the colonies, the cells and the Gram coloration in the case of bacteria were taken into account. The isolates will be molecularly identified. It is well established that only approximately 1% of microorganisms on Earth can be readily cultivated in vitro. But this work aims to use microorganisms in bioaugmentation process, so it only focused on culturable microorganisms. These preliminary results show that landfarming has an active microbiota and it is feasible to isolate microorganisms that can be used in bioaugmentation processes with native strainsFil: Salinas, A.. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Lijteroff, Rubén Enrique. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Delfini, Claudio Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Villegas, Liliana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaXL Reunión Científica Anual de la Sociedad de Biología de CuyoMendozaArgentinaSociedad de Biología de CuyoSociedad de Biología de Cuyo2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/243033Isolation and characterization of microorganisms from a landfarming of the province of san luis with potential capacity for use in bioaugmentation; XL Reunión Científica Anual de la Sociedad de Biología de Cuyo; Mendoza; Argentina; 2022; 8-8CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/wp-content/uploads/2023/10/Libro-de-Resu%CC%81menes-2022-final-2.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:27:45Zoai:ri.conicet.gov.ar:11336/243033instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:27:46.052CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Isolation and characterization of microorganisms from a landfarming of the province of san luis with potential capacity for use in bioaugmentation
title Isolation and characterization of microorganisms from a landfarming of the province of san luis with potential capacity for use in bioaugmentation
spellingShingle Isolation and characterization of microorganisms from a landfarming of the province of san luis with potential capacity for use in bioaugmentation
Salinas, A.
LANDFARMING
MICROORGANISMS
title_short Isolation and characterization of microorganisms from a landfarming of the province of san luis with potential capacity for use in bioaugmentation
title_full Isolation and characterization of microorganisms from a landfarming of the province of san luis with potential capacity for use in bioaugmentation
title_fullStr Isolation and characterization of microorganisms from a landfarming of the province of san luis with potential capacity for use in bioaugmentation
title_full_unstemmed Isolation and characterization of microorganisms from a landfarming of the province of san luis with potential capacity for use in bioaugmentation
title_sort Isolation and characterization of microorganisms from a landfarming of the province of san luis with potential capacity for use in bioaugmentation
dc.creator.none.fl_str_mv Salinas, A.
Lijteroff, Rubén Enrique
Delfini, Claudio Daniel
Villegas, Liliana Beatriz
author Salinas, A.
author_facet Salinas, A.
Lijteroff, Rubén Enrique
Delfini, Claudio Daniel
Villegas, Liliana Beatriz
author_role author
author2 Lijteroff, Rubén Enrique
Delfini, Claudio Daniel
Villegas, Liliana Beatriz
author2_role author
author
author
dc.subject.none.fl_str_mv LANDFARMING
MICROORGANISMS
topic LANDFARMING
MICROORGANISMS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The final disposal of industrial effluents with organic or inorganic compounds is a concern for companies and governments. Consequently, in recent decades, different methodologies have been used to reduce them, without causing damage to the environment, among which are bioremediation processes. Landfarming is an ex-situ bioremediation treatment process that is carried out in the upper zone of the soil. In San Luis Province there is a landfarming that receives effluents from chemical industry and has been used for more than 10 years. The aim of this work was to isolate microorganism from a landfarming soil with the capacity to tolerate the environmental stresses which can favor increasing and accelerating landfarming processes. The samples were collected in two stages, during the winter period of 2022: the first was taken from the supernatant of the effluent after its discharge; the second from the mud generated in the mixture of soil and effluent, after 5 days of discharge. The sampling points were 3 at different distances from the discharge of effluents. Two approaches were utilized to isolate microorganism from the landfarming soil: a direct isolation approach in EG medium following the streaked-on agar plate and enrichment approach in liquid medium incubated a 30°C, 150 rpm for 72 h. While, using a direct isolation approach only 12 morphotypes were isolated, using enrichment approach 15 morphotypes were isolated. To characterize the isolates, the characteristics of the colonies, the cells and the Gram coloration in the case of bacteria were taken into account. The isolates will be molecularly identified. It is well established that only approximately 1% of microorganisms on Earth can be readily cultivated in vitro. But this work aims to use microorganisms in bioaugmentation process, so it only focused on culturable microorganisms. These preliminary results show that landfarming has an active microbiota and it is feasible to isolate microorganisms that can be used in bioaugmentation processes with native strains
Fil: Salinas, A.. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Lijteroff, Rubén Enrique. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Delfini, Claudio Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Villegas, Liliana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
XL Reunión Científica Anual de la Sociedad de Biología de Cuyo
Mendoza
Argentina
Sociedad de Biología de Cuyo
description The final disposal of industrial effluents with organic or inorganic compounds is a concern for companies and governments. Consequently, in recent decades, different methodologies have been used to reduce them, without causing damage to the environment, among which are bioremediation processes. Landfarming is an ex-situ bioremediation treatment process that is carried out in the upper zone of the soil. In San Luis Province there is a landfarming that receives effluents from chemical industry and has been used for more than 10 years. The aim of this work was to isolate microorganism from a landfarming soil with the capacity to tolerate the environmental stresses which can favor increasing and accelerating landfarming processes. The samples were collected in two stages, during the winter period of 2022: the first was taken from the supernatant of the effluent after its discharge; the second from the mud generated in the mixture of soil and effluent, after 5 days of discharge. The sampling points were 3 at different distances from the discharge of effluents. Two approaches were utilized to isolate microorganism from the landfarming soil: a direct isolation approach in EG medium following the streaked-on agar plate and enrichment approach in liquid medium incubated a 30°C, 150 rpm for 72 h. While, using a direct isolation approach only 12 morphotypes were isolated, using enrichment approach 15 morphotypes were isolated. To characterize the isolates, the characteristics of the colonies, the cells and the Gram coloration in the case of bacteria were taken into account. The isolates will be molecularly identified. It is well established that only approximately 1% of microorganisms on Earth can be readily cultivated in vitro. But this work aims to use microorganisms in bioaugmentation process, so it only focused on culturable microorganisms. These preliminary results show that landfarming has an active microbiota and it is feasible to isolate microorganisms that can be used in bioaugmentation processes with native strains
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/243033
Isolation and characterization of microorganisms from a landfarming of the province of san luis with potential capacity for use in bioaugmentation; XL Reunión Científica Anual de la Sociedad de Biología de Cuyo; Mendoza; Argentina; 2022; 8-8
CONICET Digital
CONICET
url http://hdl.handle.net/11336/243033
identifier_str_mv Isolation and characterization of microorganisms from a landfarming of the province of san luis with potential capacity for use in bioaugmentation; XL Reunión Científica Anual de la Sociedad de Biología de Cuyo; Mendoza; Argentina; 2022; 8-8
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/wp-content/uploads/2023/10/Libro-de-Resu%CC%81menes-2022-final-2.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad de Biología de Cuyo
publisher.none.fl_str_mv Sociedad de Biología de Cuyo
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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