Survey and characterization of pathogenic and mycotoxigenic microorganisms in walnut (Juglans regia L.)

Autores
Magris, Gina; Carrizo, Micaela; Diaz, Mariano; Caligiore Gei, Pablo Fernando; Alaniz Zanon, Maria Silvina; Chiotta, María Laura; Principe, Analia
Año de publicación
2024
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Walnut (Juglans regia L.) is the most important nut crop in Argentina, with a cultivated area of about sixteen thousand hectares. The quality and safety of walnut production can be affected by phytopathogenic bacteria and/or mycotoxinproducing fungi. This study sought to explore the incidence of such microorganisms in a walnut plantation in Belén, a department in the province of Catamarca. Fallen fruit showed stylar end necrosis that extended towards the inner tissues and reached the kernel. These symptoms were consistent with those described for brown apical necrosis (BAN). Ten symptomatic fruits from each orchard in the plantation were disinfected with sodium hypochlorite (1% v/v) for 2 min and rinsed with sterile distilled water. Small pieces of affected tissue were excised and plated on potato dextrose agar (PDA) supplemented with streptomycin (200 mg/l). After 7 days, fungal species were isolated and identified on the basis of conidial morphology and through molecular techniques. The DNA of a selected strain named H1 was extracted, and internal transcribed spacer (ITS) regions were amplified and sequenced. A BLAST analysis of the isolate’s nucleotide sequences indicated a 97-100% similarity with reference sequences of Alternaria alternata in GenBank. Its pathogenicity was studied by inoculating a 10-μl droplet of a conidial suspension (1 × 106 conidia/ml) on the stigmatic end of walnuts. All the inoculated fruits developed apical necrosis. To validate Koch’s postulates, symptomatic tissuefrom the inoculated fruits was plated on PDA, and H1 was successfully reisolated. A Gram-negative bacterial strain was also isolated and named XVM1. It was characterized at the morphological and biochemical level, and identified as a Pantoea sp. through 16S rDNA gene sequencing and fatty acid profiling (FAMEs). These results were corroborated by MALDI-TOF MS. As with H1, walnut fruits inoculated with this bacterial strain developed characteristic BAN symptoms. To further complement all these findings, walnut-associated mycoflora was studied in the same region.Sixty-one percent of the isolated strains were identified as potential mycotoxin producers, with a high incidence of the genera Aspergillus, Alternaria, and Penicillium. Although species belonging to Aspergillus section Flavi were the most frequently isolated, low levels of aflatoxins were detected (4.4 ng/g for AFB1). The presence of BAN and aflatoxins in a commercial walnut plantation in Argentina suggests a need for improved phytosanitary control programs. Nevertheless, given the limited scope of the data presented here, epidemiological studies should be conducted to betterunderstand the impact of the disease on walnut production in Argentina.
Fil: Magris, Gina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina
Fil: Carrizo, Micaela. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Diaz, Mariano. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; Argentina
Fil: Caligiore Gei, Pablo Fernando. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alaniz Zanon, Maria Silvina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Chiotta, María Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Principe, Analia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina
LX Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research
Córdoba
Argentina
Sociedad Argentina de Bioquímica y Biología Molecular
Materia
Walnut
Pathogenic Microorganisms
Micotoxigenic Microorganisms
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/273980
Acceder
id CONICETDig_e11405ab9b5b97327f57b95d781e1da6
oai_identifier_str oai:ri.conicet.gov.ar:11336/273980
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Survey and characterization of pathogenic and mycotoxigenic microorganisms in walnut (Juglans regia L.)Magris, GinaCarrizo, MicaelaDiaz, MarianoCaligiore Gei, Pablo FernandoAlaniz Zanon, Maria SilvinaChiotta, María LauraPrincipe, AnaliaWalnutPathogenic MicroorganismsMicotoxigenic Microorganismshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Walnut (Juglans regia L.) is the most important nut crop in Argentina, with a cultivated area of about sixteen thousand hectares. The quality and safety of walnut production can be affected by phytopathogenic bacteria and/or mycotoxinproducing fungi. This study sought to explore the incidence of such microorganisms in a walnut plantation in Belén, a department in the province of Catamarca. Fallen fruit showed stylar end necrosis that extended towards the inner tissues and reached the kernel. These symptoms were consistent with those described for brown apical necrosis (BAN). Ten symptomatic fruits from each orchard in the plantation were disinfected with sodium hypochlorite (1% v/v) for 2 min and rinsed with sterile distilled water. Small pieces of affected tissue were excised and plated on potato dextrose agar (PDA) supplemented with streptomycin (200 mg/l). After 7 days, fungal species were isolated and identified on the basis of conidial morphology and through molecular techniques. The DNA of a selected strain named H1 was extracted, and internal transcribed spacer (ITS) regions were amplified and sequenced. A BLAST analysis of the isolate’s nucleotide sequences indicated a 97-100% similarity with reference sequences of Alternaria alternata in GenBank. Its pathogenicity was studied by inoculating a 10-μl droplet of a conidial suspension (1 × 106 conidia/ml) on the stigmatic end of walnuts. All the inoculated fruits developed apical necrosis. To validate Koch’s postulates, symptomatic tissuefrom the inoculated fruits was plated on PDA, and H1 was successfully reisolated. A Gram-negative bacterial strain was also isolated and named XVM1. It was characterized at the morphological and biochemical level, and identified as a Pantoea sp. through 16S rDNA gene sequencing and fatty acid profiling (FAMEs). These results were corroborated by MALDI-TOF MS. As with H1, walnut fruits inoculated with this bacterial strain developed characteristic BAN symptoms. To further complement all these findings, walnut-associated mycoflora was studied in the same region.Sixty-one percent of the isolated strains were identified as potential mycotoxin producers, with a high incidence of the genera Aspergillus, Alternaria, and Penicillium. Although species belonging to Aspergillus section Flavi were the most frequently isolated, low levels of aflatoxins were detected (4.4 ng/g for AFB1). The presence of BAN and aflatoxins in a commercial walnut plantation in Argentina suggests a need for improved phytosanitary control programs. Nevertheless, given the limited scope of the data presented here, epidemiological studies should be conducted to betterunderstand the impact of the disease on walnut production in Argentina.Fil: Magris, Gina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; ArgentinaFil: Carrizo, Micaela. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; ArgentinaFil: Diaz, Mariano. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; ArgentinaFil: Caligiore Gei, Pablo Fernando. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alaniz Zanon, Maria Silvina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; ArgentinaFil: Chiotta, María Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; ArgentinaFil: Principe, Analia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; ArgentinaLX Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology ResearchCórdobaArgentinaSociedad Argentina de Bioquímica y Biología MolecularSociedad Argentina de Bioquímica y Biología Molecular2024info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/273980Survey and characterization of pathogenic and mycotoxigenic microorganisms in walnut (Juglans regia L.); LX Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; Córdoba; Argentina; 2024; 2-3CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/chrome-extension://efaidnbmnnnibpcajpcglclefindmkaj/https://saib.org.ar/archivos/2024/abstracts-EN.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-05T09:42:50Zoai:ri.conicet.gov.ar:11336/273980instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-05 09:42:50.955CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Survey and characterization of pathogenic and mycotoxigenic microorganisms in walnut (Juglans regia L.)
title Survey and characterization of pathogenic and mycotoxigenic microorganisms in walnut (Juglans regia L.)
spellingShingle Survey and characterization of pathogenic and mycotoxigenic microorganisms in walnut (Juglans regia L.)
Magris, Gina
Walnut
Pathogenic Microorganisms
Micotoxigenic Microorganisms
title_short Survey and characterization of pathogenic and mycotoxigenic microorganisms in walnut (Juglans regia L.)
title_full Survey and characterization of pathogenic and mycotoxigenic microorganisms in walnut (Juglans regia L.)
title_fullStr Survey and characterization of pathogenic and mycotoxigenic microorganisms in walnut (Juglans regia L.)
title_full_unstemmed Survey and characterization of pathogenic and mycotoxigenic microorganisms in walnut (Juglans regia L.)
title_sort Survey and characterization of pathogenic and mycotoxigenic microorganisms in walnut (Juglans regia L.)
dc.creator.none.fl_str_mv Magris, Gina
Carrizo, Micaela
Diaz, Mariano
Caligiore Gei, Pablo Fernando
Alaniz Zanon, Maria Silvina
Chiotta, María Laura
Principe, Analia
author Magris, Gina
author_facet Magris, Gina
Carrizo, Micaela
Diaz, Mariano
Caligiore Gei, Pablo Fernando
Alaniz Zanon, Maria Silvina
Chiotta, María Laura
Principe, Analia
author_role author
author2 Carrizo, Micaela
Diaz, Mariano
Caligiore Gei, Pablo Fernando
Alaniz Zanon, Maria Silvina
Chiotta, María Laura
Principe, Analia
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Walnut
Pathogenic Microorganisms
Micotoxigenic Microorganisms
topic Walnut
Pathogenic Microorganisms
Micotoxigenic Microorganisms
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Walnut (Juglans regia L.) is the most important nut crop in Argentina, with a cultivated area of about sixteen thousand hectares. The quality and safety of walnut production can be affected by phytopathogenic bacteria and/or mycotoxinproducing fungi. This study sought to explore the incidence of such microorganisms in a walnut plantation in Belén, a department in the province of Catamarca. Fallen fruit showed stylar end necrosis that extended towards the inner tissues and reached the kernel. These symptoms were consistent with those described for brown apical necrosis (BAN). Ten symptomatic fruits from each orchard in the plantation were disinfected with sodium hypochlorite (1% v/v) for 2 min and rinsed with sterile distilled water. Small pieces of affected tissue were excised and plated on potato dextrose agar (PDA) supplemented with streptomycin (200 mg/l). After 7 days, fungal species were isolated and identified on the basis of conidial morphology and through molecular techniques. The DNA of a selected strain named H1 was extracted, and internal transcribed spacer (ITS) regions were amplified and sequenced. A BLAST analysis of the isolate’s nucleotide sequences indicated a 97-100% similarity with reference sequences of Alternaria alternata in GenBank. Its pathogenicity was studied by inoculating a 10-μl droplet of a conidial suspension (1 × 106 conidia/ml) on the stigmatic end of walnuts. All the inoculated fruits developed apical necrosis. To validate Koch’s postulates, symptomatic tissuefrom the inoculated fruits was plated on PDA, and H1 was successfully reisolated. A Gram-negative bacterial strain was also isolated and named XVM1. It was characterized at the morphological and biochemical level, and identified as a Pantoea sp. through 16S rDNA gene sequencing and fatty acid profiling (FAMEs). These results were corroborated by MALDI-TOF MS. As with H1, walnut fruits inoculated with this bacterial strain developed characteristic BAN symptoms. To further complement all these findings, walnut-associated mycoflora was studied in the same region.Sixty-one percent of the isolated strains were identified as potential mycotoxin producers, with a high incidence of the genera Aspergillus, Alternaria, and Penicillium. Although species belonging to Aspergillus section Flavi were the most frequently isolated, low levels of aflatoxins were detected (4.4 ng/g for AFB1). The presence of BAN and aflatoxins in a commercial walnut plantation in Argentina suggests a need for improved phytosanitary control programs. Nevertheless, given the limited scope of the data presented here, epidemiological studies should be conducted to betterunderstand the impact of the disease on walnut production in Argentina.
Fil: Magris, Gina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina
Fil: Carrizo, Micaela. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Diaz, Mariano. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; Argentina
Fil: Caligiore Gei, Pablo Fernando. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alaniz Zanon, Maria Silvina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Chiotta, María Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Principe, Analia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina
LX Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research
Córdoba
Argentina
Sociedad Argentina de Bioquímica y Biología Molecular
description Walnut (Juglans regia L.) is the most important nut crop in Argentina, with a cultivated area of about sixteen thousand hectares. The quality and safety of walnut production can be affected by phytopathogenic bacteria and/or mycotoxinproducing fungi. This study sought to explore the incidence of such microorganisms in a walnut plantation in Belén, a department in the province of Catamarca. Fallen fruit showed stylar end necrosis that extended towards the inner tissues and reached the kernel. These symptoms were consistent with those described for brown apical necrosis (BAN). Ten symptomatic fruits from each orchard in the plantation were disinfected with sodium hypochlorite (1% v/v) for 2 min and rinsed with sterile distilled water. Small pieces of affected tissue were excised and plated on potato dextrose agar (PDA) supplemented with streptomycin (200 mg/l). After 7 days, fungal species were isolated and identified on the basis of conidial morphology and through molecular techniques. The DNA of a selected strain named H1 was extracted, and internal transcribed spacer (ITS) regions were amplified and sequenced. A BLAST analysis of the isolate’s nucleotide sequences indicated a 97-100% similarity with reference sequences of Alternaria alternata in GenBank. Its pathogenicity was studied by inoculating a 10-μl droplet of a conidial suspension (1 × 106 conidia/ml) on the stigmatic end of walnuts. All the inoculated fruits developed apical necrosis. To validate Koch’s postulates, symptomatic tissuefrom the inoculated fruits was plated on PDA, and H1 was successfully reisolated. A Gram-negative bacterial strain was also isolated and named XVM1. It was characterized at the morphological and biochemical level, and identified as a Pantoea sp. through 16S rDNA gene sequencing and fatty acid profiling (FAMEs). These results were corroborated by MALDI-TOF MS. As with H1, walnut fruits inoculated with this bacterial strain developed characteristic BAN symptoms. To further complement all these findings, walnut-associated mycoflora was studied in the same region.Sixty-one percent of the isolated strains were identified as potential mycotoxin producers, with a high incidence of the genera Aspergillus, Alternaria, and Penicillium. Although species belonging to Aspergillus section Flavi were the most frequently isolated, low levels of aflatoxins were detected (4.4 ng/g for AFB1). The presence of BAN and aflatoxins in a commercial walnut plantation in Argentina suggests a need for improved phytosanitary control programs. Nevertheless, given the limited scope of the data presented here, epidemiological studies should be conducted to betterunderstand the impact of the disease on walnut production in Argentina.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/273980
Survey and characterization of pathogenic and mycotoxigenic microorganisms in walnut (Juglans regia L.); LX Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; Córdoba; Argentina; 2024; 2-3
CONICET Digital
CONICET
url http://hdl.handle.net/11336/273980
identifier_str_mv Survey and characterization of pathogenic and mycotoxigenic microorganisms in walnut (Juglans regia L.); LX Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; Córdoba; Argentina; 2024; 2-3
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/chrome-extension://efaidnbmnnnibpcajpcglclefindmkaj/https://saib.org.ar/archivos/2024/abstracts-EN.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Bioquímica y Biología Molecular
publisher.none.fl_str_mv Sociedad Argentina de Bioquímica y Biología Molecular
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1847977008750919680
score 13.082534

Publicaciones similares