Selection of microorganisms resistant to the effluent discharged in a landfarming in the city of San Luis
- Autores
- Salinas, Adrian; Lijteroff, Rubén Enrique; Delfini, Claudio Daniel; Villegas, Liliana Beatriz
- Año de publicación
- 2024
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Biodegradation carried out by indigenous microorganisms is one of the main mechanisms by which different contaminants can be naturally eliminated from the environment. The success of a bioremediation process depends on the intrinsic capacity of the system to trigger and maintain conditions that promote the contaminants biodegradation at high rate. The objective of this work was to study the microorganism’s resistance capacity to resin effluents for their use in the bioaugmentation process. The isolated microorganisms from landfarming, in previous works, were seeded in solid Eg medium (g L-1: glucose, 10; K2HPO4, 0.125; KH2PO4, 0.125; MgSO4, 0.1 y yeast extract,1; agar 15) pH 6,0± 0,2, incubated until growth at 29°C. A central channel was made in each plate in which the effluent diluted to 50% was added. On the other hand, the inhibition effect between the different strains of bacteria and fungi was tested. And finally, a quantitative test was carried out in 10 mL of LB glu liquid medium (g L-1: glucose, yeast extract, peptone, sodium chloride) at different concentrations of the resin effluent (25%, 50% and 75%) at 150 rpm to analyse the individual resistance of the microorganisms to the effluent. Culture medium and water were used as reference. From these tests, four resistant microorganisms (bacteria and fungi) were obtained, which did not show inhibition among themselves. Furthermore, growth of the microorganisms could be visualized at all effluent concentrations tested, being faster and greater at highest concentrations of effluent. The latter, shows that microorganisms use the effluent as a source of carbon and energy for its growth. These preliminary results show that landfarming has an active microbiota and it is feasible to isolate microorganisms that can be used in bioaugmentation processes with native strains.
Fil: Salinas, Adrian. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Lijteroff, Rubén Enrique. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Delfini, Claudio Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Villegas, Liliana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
XLI Reunión Científica Anual de la Sociedad de Biología de Cuyo
San Juan
Argentina
Sociedad de Biología de Cuyo - Materia
-
BIOREMEDIATION
LANDFARMING - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/257454
Ver los metadatos del registro completo
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Selection of microorganisms resistant to the effluent discharged in a landfarming in the city of San LuisSalinas, AdrianLijteroff, Rubén EnriqueDelfini, Claudio DanielVillegas, Liliana BeatrizBIOREMEDIATIONLANDFARMINGhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Biodegradation carried out by indigenous microorganisms is one of the main mechanisms by which different contaminants can be naturally eliminated from the environment. The success of a bioremediation process depends on the intrinsic capacity of the system to trigger and maintain conditions that promote the contaminants biodegradation at high rate. The objective of this work was to study the microorganism’s resistance capacity to resin effluents for their use in the bioaugmentation process. The isolated microorganisms from landfarming, in previous works, were seeded in solid Eg medium (g L-1: glucose, 10; K2HPO4, 0.125; KH2PO4, 0.125; MgSO4, 0.1 y yeast extract,1; agar 15) pH 6,0± 0,2, incubated until growth at 29°C. A central channel was made in each plate in which the effluent diluted to 50% was added. On the other hand, the inhibition effect between the different strains of bacteria and fungi was tested. And finally, a quantitative test was carried out in 10 mL of LB glu liquid medium (g L-1: glucose, yeast extract, peptone, sodium chloride) at different concentrations of the resin effluent (25%, 50% and 75%) at 150 rpm to analyse the individual resistance of the microorganisms to the effluent. Culture medium and water were used as reference. From these tests, four resistant microorganisms (bacteria and fungi) were obtained, which did not show inhibition among themselves. Furthermore, growth of the microorganisms could be visualized at all effluent concentrations tested, being faster and greater at highest concentrations of effluent. The latter, shows that microorganisms use the effluent as a source of carbon and energy for its growth. These preliminary results show that landfarming has an active microbiota and it is feasible to isolate microorganisms that can be used in bioaugmentation processes with native strains.Fil: Salinas, Adrian. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Lijteroff, Rubén Enrique. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Delfini, Claudio Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Villegas, Liliana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaXLI Reunión Científica Anual de la Sociedad de Biología de CuyoSan JuanArgentinaSociedad de Biología de CuyoTech Science Press2024info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/257454Selection of microorganisms resistant to the effluent discharged in a landfarming in the city of San Luis; XLI Reunión Científica Anual de la Sociedad de Biología de Cuyo; San Juan; Argentina; 2023; 1-11667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/reuniones-anuales-anteriores/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:55:50Zoai:ri.conicet.gov.ar:11336/257454instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:55:50.877CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Selection of microorganisms resistant to the effluent discharged in a landfarming in the city of San Luis |
| title |
Selection of microorganisms resistant to the effluent discharged in a landfarming in the city of San Luis |
| spellingShingle |
Selection of microorganisms resistant to the effluent discharged in a landfarming in the city of San Luis Salinas, Adrian BIOREMEDIATION LANDFARMING |
| title_short |
Selection of microorganisms resistant to the effluent discharged in a landfarming in the city of San Luis |
| title_full |
Selection of microorganisms resistant to the effluent discharged in a landfarming in the city of San Luis |
| title_fullStr |
Selection of microorganisms resistant to the effluent discharged in a landfarming in the city of San Luis |
| title_full_unstemmed |
Selection of microorganisms resistant to the effluent discharged in a landfarming in the city of San Luis |
| title_sort |
Selection of microorganisms resistant to the effluent discharged in a landfarming in the city of San Luis |
| dc.creator.none.fl_str_mv |
Salinas, Adrian Lijteroff, Rubén Enrique Delfini, Claudio Daniel Villegas, Liliana Beatriz |
| author |
Salinas, Adrian |
| author_facet |
Salinas, Adrian Lijteroff, Rubén Enrique Delfini, Claudio Daniel Villegas, Liliana Beatriz |
| author_role |
author |
| author2 |
Lijteroff, Rubén Enrique Delfini, Claudio Daniel Villegas, Liliana Beatriz |
| author2_role |
author author author |
| dc.subject.none.fl_str_mv |
BIOREMEDIATION LANDFARMING |
| topic |
BIOREMEDIATION LANDFARMING |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
Biodegradation carried out by indigenous microorganisms is one of the main mechanisms by which different contaminants can be naturally eliminated from the environment. The success of a bioremediation process depends on the intrinsic capacity of the system to trigger and maintain conditions that promote the contaminants biodegradation at high rate. The objective of this work was to study the microorganism’s resistance capacity to resin effluents for their use in the bioaugmentation process. The isolated microorganisms from landfarming, in previous works, were seeded in solid Eg medium (g L-1: glucose, 10; K2HPO4, 0.125; KH2PO4, 0.125; MgSO4, 0.1 y yeast extract,1; agar 15) pH 6,0± 0,2, incubated until growth at 29°C. A central channel was made in each plate in which the effluent diluted to 50% was added. On the other hand, the inhibition effect between the different strains of bacteria and fungi was tested. And finally, a quantitative test was carried out in 10 mL of LB glu liquid medium (g L-1: glucose, yeast extract, peptone, sodium chloride) at different concentrations of the resin effluent (25%, 50% and 75%) at 150 rpm to analyse the individual resistance of the microorganisms to the effluent. Culture medium and water were used as reference. From these tests, four resistant microorganisms (bacteria and fungi) were obtained, which did not show inhibition among themselves. Furthermore, growth of the microorganisms could be visualized at all effluent concentrations tested, being faster and greater at highest concentrations of effluent. The latter, shows that microorganisms use the effluent as a source of carbon and energy for its growth. These preliminary results show that landfarming has an active microbiota and it is feasible to isolate microorganisms that can be used in bioaugmentation processes with native strains. Fil: Salinas, Adrian. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina Fil: Lijteroff, Rubén Enrique. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina Fil: Delfini, Claudio Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina Fil: Villegas, Liliana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina XLI Reunión Científica Anual de la Sociedad de Biología de Cuyo San Juan Argentina Sociedad de Biología de Cuyo |
| description |
Biodegradation carried out by indigenous microorganisms is one of the main mechanisms by which different contaminants can be naturally eliminated from the environment. The success of a bioremediation process depends on the intrinsic capacity of the system to trigger and maintain conditions that promote the contaminants biodegradation at high rate. The objective of this work was to study the microorganism’s resistance capacity to resin effluents for their use in the bioaugmentation process. The isolated microorganisms from landfarming, in previous works, were seeded in solid Eg medium (g L-1: glucose, 10; K2HPO4, 0.125; KH2PO4, 0.125; MgSO4, 0.1 y yeast extract,1; agar 15) pH 6,0± 0,2, incubated until growth at 29°C. A central channel was made in each plate in which the effluent diluted to 50% was added. On the other hand, the inhibition effect between the different strains of bacteria and fungi was tested. And finally, a quantitative test was carried out in 10 mL of LB glu liquid medium (g L-1: glucose, yeast extract, peptone, sodium chloride) at different concentrations of the resin effluent (25%, 50% and 75%) at 150 rpm to analyse the individual resistance of the microorganisms to the effluent. Culture medium and water were used as reference. From these tests, four resistant microorganisms (bacteria and fungi) were obtained, which did not show inhibition among themselves. Furthermore, growth of the microorganisms could be visualized at all effluent concentrations tested, being faster and greater at highest concentrations of effluent. The latter, shows that microorganisms use the effluent as a source of carbon and energy for its growth. These preliminary results show that landfarming has an active microbiota and it is feasible to isolate microorganisms that can be used in bioaugmentation processes with native strains. |
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2024 |
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2024 |
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