Selection and identification of esterase-producing microorganisms

Autores
Loto, Flavia del Valle; Pera, Licia Maria; Baigori, Mario Domingo
Año de publicación
2007
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
One of the most efficient and successful means of finding new microbial enzymes is to screen a large number of microorganisms, because of their diversity and versatility. The capability of Bacillus strains to produce and to secrete enzymes has placed them among the most important industrial enzyme producers. Esterases, one of them, have important biotechnology applications, such as drug synthesis. The aim of the present study was to select and to characterize good esterase producing bacteria. Esterase-producing colonies were selected by their ability to hydrolyze á-naphthylacetate on LB plates. The selected strains were characterized with molecular and biochemical methods. Five of fifty isolations were selected because of their high levels of extracellular and cell-bound esterases production. On the basis of their biochemical and molecular properties the strains A14 y A55 appear to belong to B. pumilus, A60 y A62 to B. subtilis and M2 to B. cereus. The nucleotide sequences of the 16S rDNA are available in GenBank under the following accession numbers A14 EF462914, A55 638794, A60 EF513611, A62 EF513612 y M2 EF 513610. This characterization will provide tools for designing new enzymatic processes. PICTO-UNT 761, PIP 6062 and CIUNT 26/D308 supported this work
Fil: Loto, Flavia del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pera, Licia Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
I Reunion Conjunta de Sociedades de Biologia de la República Argentina
Huerta Grande
Argentina
Sociedades de Biologia de la República Argentina
Materia
MICROORGANISMS
ESTERASE-PRODUCING
SELECTION
IDENTIFICATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/199775
Acceder
id CONICETDig_f610249f371f56a06f1036f663c775d8
oai_identifier_str oai:ri.conicet.gov.ar:11336/199775
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Selection and identification of esterase-producing microorganismsLoto, Flavia del VallePera, Licia MariaBaigori, Mario DomingoMICROORGANISMSESTERASE-PRODUCINGSELECTIONIDENTIFICATIONhttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2One of the most efficient and successful means of finding new microbial enzymes is to screen a large number of microorganisms, because of their diversity and versatility. The capability of Bacillus strains to produce and to secrete enzymes has placed them among the most important industrial enzyme producers. Esterases, one of them, have important biotechnology applications, such as drug synthesis. The aim of the present study was to select and to characterize good esterase producing bacteria. Esterase-producing colonies were selected by their ability to hydrolyze á-naphthylacetate on LB plates. The selected strains were characterized with molecular and biochemical methods. Five of fifty isolations were selected because of their high levels of extracellular and cell-bound esterases production. On the basis of their biochemical and molecular properties the strains A14 y A55 appear to belong to B. pumilus, A60 y A62 to B. subtilis and M2 to B. cereus. The nucleotide sequences of the 16S rDNA are available in GenBank under the following accession numbers A14 EF462914, A55 638794, A60 EF513611, A62 EF513612 y M2 EF 513610. This characterization will provide tools for designing new enzymatic processes. PICTO-UNT 761, PIP 6062 and CIUNT 26/D308 supported this workFil: Loto, Flavia del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Pera, Licia Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaI Reunion Conjunta de Sociedades de Biologia de la República ArgentinaHuerta GrandeArgentinaSociedades de Biologia de la República ArgentinaTech Science Press2007info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/199775Selection and identification of esterase-producing microorganisms; I Reunion Conjunta de Sociedades de Biologia de la República Argentina; Huerta Grande; Argentina; 2007; 123-1230327-9545CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v32nSuppl.S/37978/pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:14:32Zoai:ri.conicet.gov.ar:11336/199775instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:14:32.706CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Selection and identification of esterase-producing microorganisms
title Selection and identification of esterase-producing microorganisms
spellingShingle Selection and identification of esterase-producing microorganisms
Loto, Flavia del Valle
MICROORGANISMS
ESTERASE-PRODUCING
SELECTION
IDENTIFICATION
title_short Selection and identification of esterase-producing microorganisms
title_full Selection and identification of esterase-producing microorganisms
title_fullStr Selection and identification of esterase-producing microorganisms
title_full_unstemmed Selection and identification of esterase-producing microorganisms
title_sort Selection and identification of esterase-producing microorganisms
dc.creator.none.fl_str_mv Loto, Flavia del Valle
Pera, Licia Maria
Baigori, Mario Domingo
author Loto, Flavia del Valle
author_facet Loto, Flavia del Valle
Pera, Licia Maria
Baigori, Mario Domingo
author_role author
author2 Pera, Licia Maria
Baigori, Mario Domingo
author2_role author
author
dc.subject.none.fl_str_mv MICROORGANISMS
ESTERASE-PRODUCING
SELECTION
IDENTIFICATION
topic MICROORGANISMS
ESTERASE-PRODUCING
SELECTION
IDENTIFICATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv One of the most efficient and successful means of finding new microbial enzymes is to screen a large number of microorganisms, because of their diversity and versatility. The capability of Bacillus strains to produce and to secrete enzymes has placed them among the most important industrial enzyme producers. Esterases, one of them, have important biotechnology applications, such as drug synthesis. The aim of the present study was to select and to characterize good esterase producing bacteria. Esterase-producing colonies were selected by their ability to hydrolyze á-naphthylacetate on LB plates. The selected strains were characterized with molecular and biochemical methods. Five of fifty isolations were selected because of their high levels of extracellular and cell-bound esterases production. On the basis of their biochemical and molecular properties the strains A14 y A55 appear to belong to B. pumilus, A60 y A62 to B. subtilis and M2 to B. cereus. The nucleotide sequences of the 16S rDNA are available in GenBank under the following accession numbers A14 EF462914, A55 638794, A60 EF513611, A62 EF513612 y M2 EF 513610. This characterization will provide tools for designing new enzymatic processes. PICTO-UNT 761, PIP 6062 and CIUNT 26/D308 supported this work
Fil: Loto, Flavia del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pera, Licia Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
I Reunion Conjunta de Sociedades de Biologia de la República Argentina
Huerta Grande
Argentina
Sociedades de Biologia de la República Argentina
description One of the most efficient and successful means of finding new microbial enzymes is to screen a large number of microorganisms, because of their diversity and versatility. The capability of Bacillus strains to produce and to secrete enzymes has placed them among the most important industrial enzyme producers. Esterases, one of them, have important biotechnology applications, such as drug synthesis. The aim of the present study was to select and to characterize good esterase producing bacteria. Esterase-producing colonies were selected by their ability to hydrolyze á-naphthylacetate on LB plates. The selected strains were characterized with molecular and biochemical methods. Five of fifty isolations were selected because of their high levels of extracellular and cell-bound esterases production. On the basis of their biochemical and molecular properties the strains A14 y A55 appear to belong to B. pumilus, A60 y A62 to B. subtilis and M2 to B. cereus. The nucleotide sequences of the 16S rDNA are available in GenBank under the following accession numbers A14 EF462914, A55 638794, A60 EF513611, A62 EF513612 y M2 EF 513610. This characterization will provide tools for designing new enzymatic processes. PICTO-UNT 761, PIP 6062 and CIUNT 26/D308 supported this work
publishDate 2007
dc.date.none.fl_str_mv 2007
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/199775
Selection and identification of esterase-producing microorganisms; I Reunion Conjunta de Sociedades de Biologia de la República Argentina; Huerta Grande; Argentina; 2007; 123-123
0327-9545
CONICET Digital
CONICET
url http://hdl.handle.net/11336/199775
identifier_str_mv Selection and identification of esterase-producing microorganisms; I Reunion Conjunta de Sociedades de Biologia de la República Argentina; Huerta Grande; Argentina; 2007; 123-123
0327-9545
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v32nSuppl.S/37978/pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782553882624000
score 12.982451

Publicaciones similares