Selection and identification of esterase-producing microorganisms
- Autores
- Loto, Flavia del Valle; Pera, Licia Maria; Baigori, Mario Domingo
- Año de publicación
- 2007
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- One of the most efficient and successful means of finding new microbial enzymes is to screen a large number of microorganisms, because of their diversity and versatility. The capability of Bacillus strains to produce and to secrete enzymes has placed them among the most important industrial enzyme producers. Esterases, one of them, have important biotechnology applications, such as drug synthesis. The aim of the present study was to select and to characterize good esterase producing bacteria. Esterase-producing colonies were selected by their ability to hydrolyze á-naphthylacetate on LB plates. The selected strains were characterized with molecular and biochemical methods. Five of fifty isolations were selected because of their high levels of extracellular and cell-bound esterases production. On the basis of their biochemical and molecular properties the strains A14 y A55 appear to belong to B. pumilus, A60 y A62 to B. subtilis and M2 to B. cereus. The nucleotide sequences of the 16S rDNA are available in GenBank under the following accession numbers A14 EF462914, A55 638794, A60 EF513611, A62 EF513612 y M2 EF 513610. This characterization will provide tools for designing new enzymatic processes. PICTO-UNT 761, PIP 6062 and CIUNT 26/D308 supported this work
Fil: Loto, Flavia del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pera, Licia Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
I Reunion Conjunta de Sociedades de Biologia de la República Argentina
Huerta Grande
Argentina
Sociedades de Biologia de la República Argentina - Materia
-
MICROORGANISMS
ESTERASE-PRODUCING
SELECTION
IDENTIFICATION - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/199775
Ver los metadatos del registro completo
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Selection and identification of esterase-producing microorganismsLoto, Flavia del VallePera, Licia MariaBaigori, Mario DomingoMICROORGANISMSESTERASE-PRODUCINGSELECTIONIDENTIFICATIONhttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2One of the most efficient and successful means of finding new microbial enzymes is to screen a large number of microorganisms, because of their diversity and versatility. The capability of Bacillus strains to produce and to secrete enzymes has placed them among the most important industrial enzyme producers. Esterases, one of them, have important biotechnology applications, such as drug synthesis. The aim of the present study was to select and to characterize good esterase producing bacteria. Esterase-producing colonies were selected by their ability to hydrolyze á-naphthylacetate on LB plates. The selected strains were characterized with molecular and biochemical methods. Five of fifty isolations were selected because of their high levels of extracellular and cell-bound esterases production. On the basis of their biochemical and molecular properties the strains A14 y A55 appear to belong to B. pumilus, A60 y A62 to B. subtilis and M2 to B. cereus. The nucleotide sequences of the 16S rDNA are available in GenBank under the following accession numbers A14 EF462914, A55 638794, A60 EF513611, A62 EF513612 y M2 EF 513610. This characterization will provide tools for designing new enzymatic processes. PICTO-UNT 761, PIP 6062 and CIUNT 26/D308 supported this workFil: Loto, Flavia del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Pera, Licia Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaI Reunion Conjunta de Sociedades de Biologia de la República ArgentinaHuerta GrandeArgentinaSociedades de Biologia de la República ArgentinaTech Science Press2007info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/199775Selection and identification of esterase-producing microorganisms; I Reunion Conjunta de Sociedades de Biologia de la República Argentina; Huerta Grande; Argentina; 2007; 123-1230327-9545CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v32nSuppl.S/37978/pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:41:53Zoai:ri.conicet.gov.ar:11336/199775instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:41:53.738CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Selection and identification of esterase-producing microorganisms |
title |
Selection and identification of esterase-producing microorganisms |
spellingShingle |
Selection and identification of esterase-producing microorganisms Loto, Flavia del Valle MICROORGANISMS ESTERASE-PRODUCING SELECTION IDENTIFICATION |
title_short |
Selection and identification of esterase-producing microorganisms |
title_full |
Selection and identification of esterase-producing microorganisms |
title_fullStr |
Selection and identification of esterase-producing microorganisms |
title_full_unstemmed |
Selection and identification of esterase-producing microorganisms |
title_sort |
Selection and identification of esterase-producing microorganisms |
dc.creator.none.fl_str_mv |
Loto, Flavia del Valle Pera, Licia Maria Baigori, Mario Domingo |
author |
Loto, Flavia del Valle |
author_facet |
Loto, Flavia del Valle Pera, Licia Maria Baigori, Mario Domingo |
author_role |
author |
author2 |
Pera, Licia Maria Baigori, Mario Domingo |
author2_role |
author author |
dc.subject.none.fl_str_mv |
MICROORGANISMS ESTERASE-PRODUCING SELECTION IDENTIFICATION |
topic |
MICROORGANISMS ESTERASE-PRODUCING SELECTION IDENTIFICATION |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.11 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
One of the most efficient and successful means of finding new microbial enzymes is to screen a large number of microorganisms, because of their diversity and versatility. The capability of Bacillus strains to produce and to secrete enzymes has placed them among the most important industrial enzyme producers. Esterases, one of them, have important biotechnology applications, such as drug synthesis. The aim of the present study was to select and to characterize good esterase producing bacteria. Esterase-producing colonies were selected by their ability to hydrolyze á-naphthylacetate on LB plates. The selected strains were characterized with molecular and biochemical methods. Five of fifty isolations were selected because of their high levels of extracellular and cell-bound esterases production. On the basis of their biochemical and molecular properties the strains A14 y A55 appear to belong to B. pumilus, A60 y A62 to B. subtilis and M2 to B. cereus. The nucleotide sequences of the 16S rDNA are available in GenBank under the following accession numbers A14 EF462914, A55 638794, A60 EF513611, A62 EF513612 y M2 EF 513610. This characterization will provide tools for designing new enzymatic processes. PICTO-UNT 761, PIP 6062 and CIUNT 26/D308 supported this work Fil: Loto, Flavia del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina Fil: Pera, Licia Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina Fil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina I Reunion Conjunta de Sociedades de Biologia de la República Argentina Huerta Grande Argentina Sociedades de Biologia de la República Argentina |
description |
One of the most efficient and successful means of finding new microbial enzymes is to screen a large number of microorganisms, because of their diversity and versatility. The capability of Bacillus strains to produce and to secrete enzymes has placed them among the most important industrial enzyme producers. Esterases, one of them, have important biotechnology applications, such as drug synthesis. The aim of the present study was to select and to characterize good esterase producing bacteria. Esterase-producing colonies were selected by their ability to hydrolyze á-naphthylacetate on LB plates. The selected strains were characterized with molecular and biochemical methods. Five of fifty isolations were selected because of their high levels of extracellular and cell-bound esterases production. On the basis of their biochemical and molecular properties the strains A14 y A55 appear to belong to B. pumilus, A60 y A62 to B. subtilis and M2 to B. cereus. The nucleotide sequences of the 16S rDNA are available in GenBank under the following accession numbers A14 EF462914, A55 638794, A60 EF513611, A62 EF513612 y M2 EF 513610. This characterization will provide tools for designing new enzymatic processes. PICTO-UNT 761, PIP 6062 and CIUNT 26/D308 supported this work |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Reunión Journal http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
status_str |
publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/199775 Selection and identification of esterase-producing microorganisms; I Reunion Conjunta de Sociedades de Biologia de la República Argentina; Huerta Grande; Argentina; 2007; 123-123 0327-9545 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/199775 |
identifier_str_mv |
Selection and identification of esterase-producing microorganisms; I Reunion Conjunta de Sociedades de Biologia de la República Argentina; Huerta Grande; Argentina; 2007; 123-123 0327-9545 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v32nSuppl.S/37978/pdf |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.coverage.none.fl_str_mv |
Nacional |
dc.publisher.none.fl_str_mv |
Tech Science Press |
publisher.none.fl_str_mv |
Tech Science Press |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614450736267264 |
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13.069144 |