Characterization of new strains of Pseudorabies virus in Argentina: Detection of interspecies transmission

Autores
Serena, Maria Soledad; Cappuccio, Javier Alejandro; Fossaroli, Melisa Gisele; Williman, Macarena Marta; Dibárbora, Marina; Brizzio, Renata; Metz, German Ernesto; Aspitia, Carolina Gabriela; Pérez, Alejandro; Carpinetti, Bruno Nicolás; Echeverria, Maria Gabriela
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Aujeszky's disease is mainly a swine disease, still endemic worldwide. It can infect other mammalians, including human beings, and it is usually fatal with nervous symptoms. Ever since the disease was detected in 1988 in Argentina, many outbreaks have been reported involving both feral swine and dogs. Aim: At present, in Argentina, Pseudorabies virus (PRV) cases are sporadically reported; however, clinical cases are informed. This study aims to obtain information about the seroprevalence of PRV in wild boars and to isolate and characterize PRV from clinical samples. Methods: From 2018 to 2019, 78 wild boars’ serum samples from Bahía de Samborombón natural reserve were analyzed for antibodies to PRV using a virus neutralization test. Clinical samples from 17 pigs, 2 wild boars, 1 dog, and 1 cat were collected from 2013 to 2019 for viral isolation and detection of the presence of the gD gene by PCR. For sequence analysis, the gC partial gene was amplified. Results: Five strains were isolated from the dog, cat, and swine samples. The new PRV strains identified were confirmed by BLAST analysis, which revealed between 99.74% and 100% of similarity to the NIA-3 strain and phylogenetic analysis of the partial gene encoding the gC protein revealed that the PRV strains have divided into two main clades, clade 1 and clade 2. Conclusion: This report informed that most new cases of PRV were detected in the central regions of Argentina, where pig production is concentrated. The study in Bahía de Samborombón revealed a high percentage of detection but, the sampling is not representative of that of the rest of the country. Therefore, a systematic sampling effort of wild boar throughout the country should be included in the national program control. Although in Argentina only the inactivated Bartha vaccine is allowed, recombination risk should not be ignored if attenuated vaccines are incorporated into the National control plan. The two strains, one from the cat and one from the dog sample, are directly related to infected swine. The information about clinical cases and molecular characterization of new strains is important for a better understanding of the dynamics of PRV and to promote preventive measures.
Fil: Serena, Maria Soledad. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Cappuccio, Javier Alejandro. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Fossaroli, Melisa Gisele. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias; Argentina
Fil: Williman, Macarena Marta. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Dibárbora, Marina. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Brizzio, Renata. Universidad Católica de Córdoba; Argentina
Fil: Metz, German Ernesto. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Aspitia, Carolina Gabriela. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Pérez, Alejandro. Ministerio de Agricultura, Ganadería, Pesca y Alimento. Servicio Nacional de Sanidad y Calidad Agroalimentaria; Argentina
Fil: Carpinetti, Bruno Nicolás. Universidad Nacional Arturo Jauretche. Instituto Ciencias Sociales y Administracion; Argentina
Fil: Echeverria, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina
Materia
ARGENTINA
AUJESZKY'S DISEASE
PSEUDORABIES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/221995
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/221995
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization of new strains of Pseudorabies virus in Argentina: Detection of interspecies transmissionSerena, Maria SoledadCappuccio, Javier AlejandroFossaroli, Melisa GiseleWilliman, Macarena MartaDibárbora, MarinaBrizzio, RenataMetz, German ErnestoAspitia, Carolina GabrielaPérez, AlejandroCarpinetti, Bruno NicolásEcheverria, Maria GabrielaARGENTINAAUJESZKY'S DISEASEPSEUDORABIEShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Background: Aujeszky's disease is mainly a swine disease, still endemic worldwide. It can infect other mammalians, including human beings, and it is usually fatal with nervous symptoms. Ever since the disease was detected in 1988 in Argentina, many outbreaks have been reported involving both feral swine and dogs. Aim: At present, in Argentina, Pseudorabies virus (PRV) cases are sporadically reported; however, clinical cases are informed. This study aims to obtain information about the seroprevalence of PRV in wild boars and to isolate and characterize PRV from clinical samples. Methods: From 2018 to 2019, 78 wild boars’ serum samples from Bahía de Samborombón natural reserve were analyzed for antibodies to PRV using a virus neutralization test. Clinical samples from 17 pigs, 2 wild boars, 1 dog, and 1 cat were collected from 2013 to 2019 for viral isolation and detection of the presence of the gD gene by PCR. For sequence analysis, the gC partial gene was amplified. Results: Five strains were isolated from the dog, cat, and swine samples. The new PRV strains identified were confirmed by BLAST analysis, which revealed between 99.74% and 100% of similarity to the NIA-3 strain and phylogenetic analysis of the partial gene encoding the gC protein revealed that the PRV strains have divided into two main clades, clade 1 and clade 2. Conclusion: This report informed that most new cases of PRV were detected in the central regions of Argentina, where pig production is concentrated. The study in Bahía de Samborombón revealed a high percentage of detection but, the sampling is not representative of that of the rest of the country. Therefore, a systematic sampling effort of wild boar throughout the country should be included in the national program control. Although in Argentina only the inactivated Bartha vaccine is allowed, recombination risk should not be ignored if attenuated vaccines are incorporated into the National control plan. The two strains, one from the cat and one from the dog sample, are directly related to infected swine. The information about clinical cases and molecular characterization of new strains is important for a better understanding of the dynamics of PRV and to promote preventive measures.Fil: Serena, Maria Soledad. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Cappuccio, Javier Alejandro. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Fossaroli, Melisa Gisele. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias; ArgentinaFil: Williman, Macarena Marta. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Dibárbora, Marina. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Brizzio, Renata. Universidad Católica de Córdoba; ArgentinaFil: Metz, German Ernesto. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Aspitia, Carolina Gabriela. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Pérez, Alejandro. Ministerio de Agricultura, Ganadería, Pesca y Alimento. Servicio Nacional de Sanidad y Calidad Agroalimentaria; ArgentinaFil: Carpinetti, Bruno Nicolás. Universidad Nacional Arturo Jauretche. Instituto Ciencias Sociales y Administracion; ArgentinaFil: Echeverria, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; ArgentinaUniversity of Tripoli. Faculty of Veterinary Medicine2023-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/221995Serena, Maria Soledad; Cappuccio, Javier Alejandro; Fossaroli, Melisa Gisele; Williman, Macarena Marta; Dibárbora, Marina; et al.; Characterization of new strains of Pseudorabies virus in Argentina: Detection of interspecies transmission; University of Tripoli. Faculty of Veterinary Medicine; Open Veterinary Journal; 13; 4; 4-2023; 419-4262226-44852218-6050CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.ejmanager.com/mnstemps/100/100-1666108780.pdf?t=1683641044info:eu-repo/semantics/altIdentifier/doi/10.5455/OVJ.2023.v13.i4.3info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:00:17Zoai:ri.conicet.gov.ar:11336/221995instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:00:17.738CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of new strains of Pseudorabies virus in Argentina: Detection of interspecies transmission
title Characterization of new strains of Pseudorabies virus in Argentina: Detection of interspecies transmission
spellingShingle Characterization of new strains of Pseudorabies virus in Argentina: Detection of interspecies transmission
Serena, Maria Soledad
ARGENTINA
AUJESZKY'S DISEASE
PSEUDORABIES
title_short Characterization of new strains of Pseudorabies virus in Argentina: Detection of interspecies transmission
title_full Characterization of new strains of Pseudorabies virus in Argentina: Detection of interspecies transmission
title_fullStr Characterization of new strains of Pseudorabies virus in Argentina: Detection of interspecies transmission
title_full_unstemmed Characterization of new strains of Pseudorabies virus in Argentina: Detection of interspecies transmission
title_sort Characterization of new strains of Pseudorabies virus in Argentina: Detection of interspecies transmission
dc.creator.none.fl_str_mv Serena, Maria Soledad
Cappuccio, Javier Alejandro
Fossaroli, Melisa Gisele
Williman, Macarena Marta
Dibárbora, Marina
Brizzio, Renata
Metz, German Ernesto
Aspitia, Carolina Gabriela
Pérez, Alejandro
Carpinetti, Bruno Nicolás
Echeverria, Maria Gabriela
author Serena, Maria Soledad
author_facet Serena, Maria Soledad
Cappuccio, Javier Alejandro
Fossaroli, Melisa Gisele
Williman, Macarena Marta
Dibárbora, Marina
Brizzio, Renata
Metz, German Ernesto
Aspitia, Carolina Gabriela
Pérez, Alejandro
Carpinetti, Bruno Nicolás
Echeverria, Maria Gabriela
author_role author
author2 Cappuccio, Javier Alejandro
Fossaroli, Melisa Gisele
Williman, Macarena Marta
Dibárbora, Marina
Brizzio, Renata
Metz, German Ernesto
Aspitia, Carolina Gabriela
Pérez, Alejandro
Carpinetti, Bruno Nicolás
Echeverria, Maria Gabriela
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ARGENTINA
AUJESZKY'S DISEASE
PSEUDORABIES
topic ARGENTINA
AUJESZKY'S DISEASE
PSEUDORABIES
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Background: Aujeszky's disease is mainly a swine disease, still endemic worldwide. It can infect other mammalians, including human beings, and it is usually fatal with nervous symptoms. Ever since the disease was detected in 1988 in Argentina, many outbreaks have been reported involving both feral swine and dogs. Aim: At present, in Argentina, Pseudorabies virus (PRV) cases are sporadically reported; however, clinical cases are informed. This study aims to obtain information about the seroprevalence of PRV in wild boars and to isolate and characterize PRV from clinical samples. Methods: From 2018 to 2019, 78 wild boars’ serum samples from Bahía de Samborombón natural reserve were analyzed for antibodies to PRV using a virus neutralization test. Clinical samples from 17 pigs, 2 wild boars, 1 dog, and 1 cat were collected from 2013 to 2019 for viral isolation and detection of the presence of the gD gene by PCR. For sequence analysis, the gC partial gene was amplified. Results: Five strains were isolated from the dog, cat, and swine samples. The new PRV strains identified were confirmed by BLAST analysis, which revealed between 99.74% and 100% of similarity to the NIA-3 strain and phylogenetic analysis of the partial gene encoding the gC protein revealed that the PRV strains have divided into two main clades, clade 1 and clade 2. Conclusion: This report informed that most new cases of PRV were detected in the central regions of Argentina, where pig production is concentrated. The study in Bahía de Samborombón revealed a high percentage of detection but, the sampling is not representative of that of the rest of the country. Therefore, a systematic sampling effort of wild boar throughout the country should be included in the national program control. Although in Argentina only the inactivated Bartha vaccine is allowed, recombination risk should not be ignored if attenuated vaccines are incorporated into the National control plan. The two strains, one from the cat and one from the dog sample, are directly related to infected swine. The information about clinical cases and molecular characterization of new strains is important for a better understanding of the dynamics of PRV and to promote preventive measures.
Fil: Serena, Maria Soledad. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Cappuccio, Javier Alejandro. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Fossaroli, Melisa Gisele. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias; Argentina
Fil: Williman, Macarena Marta. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Dibárbora, Marina. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Brizzio, Renata. Universidad Católica de Córdoba; Argentina
Fil: Metz, German Ernesto. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Aspitia, Carolina Gabriela. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Pérez, Alejandro. Ministerio de Agricultura, Ganadería, Pesca y Alimento. Servicio Nacional de Sanidad y Calidad Agroalimentaria; Argentina
Fil: Carpinetti, Bruno Nicolás. Universidad Nacional Arturo Jauretche. Instituto Ciencias Sociales y Administracion; Argentina
Fil: Echeverria, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina
description Background: Aujeszky's disease is mainly a swine disease, still endemic worldwide. It can infect other mammalians, including human beings, and it is usually fatal with nervous symptoms. Ever since the disease was detected in 1988 in Argentina, many outbreaks have been reported involving both feral swine and dogs. Aim: At present, in Argentina, Pseudorabies virus (PRV) cases are sporadically reported; however, clinical cases are informed. This study aims to obtain information about the seroprevalence of PRV in wild boars and to isolate and characterize PRV from clinical samples. Methods: From 2018 to 2019, 78 wild boars’ serum samples from Bahía de Samborombón natural reserve were analyzed for antibodies to PRV using a virus neutralization test. Clinical samples from 17 pigs, 2 wild boars, 1 dog, and 1 cat were collected from 2013 to 2019 for viral isolation and detection of the presence of the gD gene by PCR. For sequence analysis, the gC partial gene was amplified. Results: Five strains were isolated from the dog, cat, and swine samples. The new PRV strains identified were confirmed by BLAST analysis, which revealed between 99.74% and 100% of similarity to the NIA-3 strain and phylogenetic analysis of the partial gene encoding the gC protein revealed that the PRV strains have divided into two main clades, clade 1 and clade 2. Conclusion: This report informed that most new cases of PRV were detected in the central regions of Argentina, where pig production is concentrated. The study in Bahía de Samborombón revealed a high percentage of detection but, the sampling is not representative of that of the rest of the country. Therefore, a systematic sampling effort of wild boar throughout the country should be included in the national program control. Although in Argentina only the inactivated Bartha vaccine is allowed, recombination risk should not be ignored if attenuated vaccines are incorporated into the National control plan. The two strains, one from the cat and one from the dog sample, are directly related to infected swine. The information about clinical cases and molecular characterization of new strains is important for a better understanding of the dynamics of PRV and to promote preventive measures.
publishDate 2023
dc.date.none.fl_str_mv 2023-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/221995
Serena, Maria Soledad; Cappuccio, Javier Alejandro; Fossaroli, Melisa Gisele; Williman, Macarena Marta; Dibárbora, Marina; et al.; Characterization of new strains of Pseudorabies virus in Argentina: Detection of interspecies transmission; University of Tripoli. Faculty of Veterinary Medicine; Open Veterinary Journal; 13; 4; 4-2023; 419-426
2226-4485
2218-6050
CONICET Digital
CONICET
url http://hdl.handle.net/11336/221995
identifier_str_mv Serena, Maria Soledad; Cappuccio, Javier Alejandro; Fossaroli, Melisa Gisele; Williman, Macarena Marta; Dibárbora, Marina; et al.; Characterization of new strains of Pseudorabies virus in Argentina: Detection of interspecies transmission; University of Tripoli. Faculty of Veterinary Medicine; Open Veterinary Journal; 13; 4; 4-2023; 419-426
2226-4485
2218-6050
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.ejmanager.com/mnstemps/100/100-1666108780.pdf?t=1683641044
info:eu-repo/semantics/altIdentifier/doi/10.5455/OVJ.2023.v13.i4.3
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv University of Tripoli. Faculty of Veterinary Medicine
publisher.none.fl_str_mv University of Tripoli. Faculty of Veterinary Medicine
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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