Polysaccharides from Padina tetrastromatica: Structural features, chemical modification and antiviral activity

Autores
Karmakar, Paramita; Pujol, Carlos Alberto; Damonte, Elsa Beatriz; Ghosh, Tuhin; Ray, Bimalendu
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Many viruses display affinity for cell surface heparan sulfate proteoglycans with biological relevance in virus entry. This raises the possibility of the application of sulfated polysaccharides in antiviral therapy. In this study, we have analyzed polysaccharide containing fractions derived from Padina tetrastromatica. A glucan containing α-(1 → 4)-linked glucopyranosyl residues, an alginate with apparent molecular mass of 50 kDa, and a fucoidan were isolated. Further sulfated derivatives of the fucoidan (S1-S3), which contained 0.8-1.2 sulfate groups per monomer unit showed inhibitory activity against herpes simplex virus (HSV) types 1 and 2. Their inhibitory concentration 50% (IC50) values were in the range 0.30-1.05 μg/ml and they lacked cytotoxicity at concentrations up to 1000 μg/ml. The antiviral effect was exerted during virus adsorption to the cell. The degree of sulfation seemed to play an important role because further sulfation of the isolated macromolecules largely influences their in vitro anti-HSV activity. © 2009 Elsevier Ltd. All rights reserved.
Fil: Karmakar, Paramita. University Of Burdwan; India
Fil: Pujol, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
Fil: Damonte, Elsa Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
Fil: Ghosh, Tuhin. University Of Burdwan; India
Fil: Ray, Bimalendu. University Of Burdwan; India
Materia
Anti-Hsv Activity
Dictyotaceae
Padina Tetrastromatica
Polysaccharides
Sulfation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/66378
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Polysaccharides from Padina tetrastromatica: Structural features, chemical modification and antiviral activityKarmakar, ParamitaPujol, Carlos AlbertoDamonte, Elsa BeatrizGhosh, TuhinRay, BimalenduAnti-Hsv ActivityDictyotaceaePadina TetrastromaticaPolysaccharidesSulfationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Many viruses display affinity for cell surface heparan sulfate proteoglycans with biological relevance in virus entry. This raises the possibility of the application of sulfated polysaccharides in antiviral therapy. In this study, we have analyzed polysaccharide containing fractions derived from Padina tetrastromatica. A glucan containing α-(1 → 4)-linked glucopyranosyl residues, an alginate with apparent molecular mass of 50 kDa, and a fucoidan were isolated. Further sulfated derivatives of the fucoidan (S1-S3), which contained 0.8-1.2 sulfate groups per monomer unit showed inhibitory activity against herpes simplex virus (HSV) types 1 and 2. Their inhibitory concentration 50% (IC50) values were in the range 0.30-1.05 μg/ml and they lacked cytotoxicity at concentrations up to 1000 μg/ml. The antiviral effect was exerted during virus adsorption to the cell. The degree of sulfation seemed to play an important role because further sulfation of the isolated macromolecules largely influences their in vitro anti-HSV activity. © 2009 Elsevier Ltd. All rights reserved.Fil: Karmakar, Paramita. University Of Burdwan; IndiaFil: Pujol, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; ArgentinaFil: Damonte, Elsa Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; ArgentinaFil: Ghosh, Tuhin. University Of Burdwan; IndiaFil: Ray, Bimalendu. University Of Burdwan; IndiaElsevier2010-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/66378Karmakar, Paramita; Pujol, Carlos Alberto; Damonte, Elsa Beatriz; Ghosh, Tuhin; Ray, Bimalendu; Polysaccharides from Padina tetrastromatica: Structural features, chemical modification and antiviral activity; Elsevier; Carbohydrate Polymers; 80; 2; 4-2010; 514-5210144-8617CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0144861709007176info:eu-repo/semantics/altIdentifier/doi/10.1016/j.carbpol.2009.12.014info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:47:20Zoai:ri.conicet.gov.ar:11336/66378instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:47:21.189CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Polysaccharides from Padina tetrastromatica: Structural features, chemical modification and antiviral activity
title Polysaccharides from Padina tetrastromatica: Structural features, chemical modification and antiviral activity
spellingShingle Polysaccharides from Padina tetrastromatica: Structural features, chemical modification and antiviral activity
Karmakar, Paramita
Anti-Hsv Activity
Dictyotaceae
Padina Tetrastromatica
Polysaccharides
Sulfation
title_short Polysaccharides from Padina tetrastromatica: Structural features, chemical modification and antiviral activity
title_full Polysaccharides from Padina tetrastromatica: Structural features, chemical modification and antiviral activity
title_fullStr Polysaccharides from Padina tetrastromatica: Structural features, chemical modification and antiviral activity
title_full_unstemmed Polysaccharides from Padina tetrastromatica: Structural features, chemical modification and antiviral activity
title_sort Polysaccharides from Padina tetrastromatica: Structural features, chemical modification and antiviral activity
dc.creator.none.fl_str_mv Karmakar, Paramita
Pujol, Carlos Alberto
Damonte, Elsa Beatriz
Ghosh, Tuhin
Ray, Bimalendu
author Karmakar, Paramita
author_facet Karmakar, Paramita
Pujol, Carlos Alberto
Damonte, Elsa Beatriz
Ghosh, Tuhin
Ray, Bimalendu
author_role author
author2 Pujol, Carlos Alberto
Damonte, Elsa Beatriz
Ghosh, Tuhin
Ray, Bimalendu
author2_role author
author
author
author
dc.subject.none.fl_str_mv Anti-Hsv Activity
Dictyotaceae
Padina Tetrastromatica
Polysaccharides
Sulfation
topic Anti-Hsv Activity
Dictyotaceae
Padina Tetrastromatica
Polysaccharides
Sulfation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Many viruses display affinity for cell surface heparan sulfate proteoglycans with biological relevance in virus entry. This raises the possibility of the application of sulfated polysaccharides in antiviral therapy. In this study, we have analyzed polysaccharide containing fractions derived from Padina tetrastromatica. A glucan containing α-(1 → 4)-linked glucopyranosyl residues, an alginate with apparent molecular mass of 50 kDa, and a fucoidan were isolated. Further sulfated derivatives of the fucoidan (S1-S3), which contained 0.8-1.2 sulfate groups per monomer unit showed inhibitory activity against herpes simplex virus (HSV) types 1 and 2. Their inhibitory concentration 50% (IC50) values were in the range 0.30-1.05 μg/ml and they lacked cytotoxicity at concentrations up to 1000 μg/ml. The antiviral effect was exerted during virus adsorption to the cell. The degree of sulfation seemed to play an important role because further sulfation of the isolated macromolecules largely influences their in vitro anti-HSV activity. © 2009 Elsevier Ltd. All rights reserved.
Fil: Karmakar, Paramita. University Of Burdwan; India
Fil: Pujol, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
Fil: Damonte, Elsa Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
Fil: Ghosh, Tuhin. University Of Burdwan; India
Fil: Ray, Bimalendu. University Of Burdwan; India
description Many viruses display affinity for cell surface heparan sulfate proteoglycans with biological relevance in virus entry. This raises the possibility of the application of sulfated polysaccharides in antiviral therapy. In this study, we have analyzed polysaccharide containing fractions derived from Padina tetrastromatica. A glucan containing α-(1 → 4)-linked glucopyranosyl residues, an alginate with apparent molecular mass of 50 kDa, and a fucoidan were isolated. Further sulfated derivatives of the fucoidan (S1-S3), which contained 0.8-1.2 sulfate groups per monomer unit showed inhibitory activity against herpes simplex virus (HSV) types 1 and 2. Their inhibitory concentration 50% (IC50) values were in the range 0.30-1.05 μg/ml and they lacked cytotoxicity at concentrations up to 1000 μg/ml. The antiviral effect was exerted during virus adsorption to the cell. The degree of sulfation seemed to play an important role because further sulfation of the isolated macromolecules largely influences their in vitro anti-HSV activity. © 2009 Elsevier Ltd. All rights reserved.
publishDate 2010
dc.date.none.fl_str_mv 2010-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/66378
Karmakar, Paramita; Pujol, Carlos Alberto; Damonte, Elsa Beatriz; Ghosh, Tuhin; Ray, Bimalendu; Polysaccharides from Padina tetrastromatica: Structural features, chemical modification and antiviral activity; Elsevier; Carbohydrate Polymers; 80; 2; 4-2010; 514-521
0144-8617
CONICET Digital
CONICET
url http://hdl.handle.net/11336/66378
identifier_str_mv Karmakar, Paramita; Pujol, Carlos Alberto; Damonte, Elsa Beatriz; Ghosh, Tuhin; Ray, Bimalendu; Polysaccharides from Padina tetrastromatica: Structural features, chemical modification and antiviral activity; Elsevier; Carbohydrate Polymers; 80; 2; 4-2010; 514-521
0144-8617
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0144861709007176
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.carbpol.2009.12.014
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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