Xylans from Scinaia hatei: Structural features, sulfation and anti-HSV activity

Autores
Mandal, Pinaki; Pujol, Carlos Alberto; Damonte, Elsa Beatriz; Ghosh, Tuhin; Ray, Bimalendu
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Natural compounds offer interesting pharmacological perspectives for antiviral drug development with regard to broad-spectrum antiviral properties and novel modes of action. In this study, we have analyzed alkali-extracted xylan of Scinaia hatei. Alkali extraction of this red alga yielded a xylan shown to have a molecular mass of 120kDa and a linear structure of (1→4)-linked β-d-xylopyranosyl residues. Derivatives (S1, S2, S3 and S4) generated by chemical sulfation from this macromolecule had degree of sulfation between 0.93 and 1.95, and contained strong anti-HSV activity with inhibitory concentration 50% (IC50) from 0.22 to 1.37μg/ml. Furthermore, they had no direct inactivating effect on virions in a virucidal assay. Sulfate groups account for their in vitro antiviral activity. Interestingly, sulfated xylans already exerted anti-HSV activity when only pre-incubated with the cultured cells prior to infection, thus pointing to a main inhibitory effect on viral entry. © 2009 Elsevier B.V.
Fil: Mandal, Pinaki. University Of Burdwan; India
Fil: Pujol, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
Fil: Damonte, Elsa Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
Fil: Ghosh, Tuhin. University Of Burdwan; India
Fil: Ray, Bimalendu. University Of Burdwan; India
Materia
Antiviral Activity
Herpes Simplex Virus
Liagoraceae
Scinaia Hatei
Sulfation
Xylan
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/66379
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Xylans from Scinaia hatei: Structural features, sulfation and anti-HSV activityMandal, PinakiPujol, Carlos AlbertoDamonte, Elsa BeatrizGhosh, TuhinRay, BimalenduAntiviral ActivityHerpes Simplex VirusLiagoraceaeScinaia HateiSulfationXylanhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Natural compounds offer interesting pharmacological perspectives for antiviral drug development with regard to broad-spectrum antiviral properties and novel modes of action. In this study, we have analyzed alkali-extracted xylan of Scinaia hatei. Alkali extraction of this red alga yielded a xylan shown to have a molecular mass of 120kDa and a linear structure of (1→4)-linked β-d-xylopyranosyl residues. Derivatives (S1, S2, S3 and S4) generated by chemical sulfation from this macromolecule had degree of sulfation between 0.93 and 1.95, and contained strong anti-HSV activity with inhibitory concentration 50% (IC50) from 0.22 to 1.37μg/ml. Furthermore, they had no direct inactivating effect on virions in a virucidal assay. Sulfate groups account for their in vitro antiviral activity. Interestingly, sulfated xylans already exerted anti-HSV activity when only pre-incubated with the cultured cells prior to infection, thus pointing to a main inhibitory effect on viral entry. © 2009 Elsevier B.V.Fil: Mandal, Pinaki. University Of Burdwan; IndiaFil: Pujol, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; ArgentinaFil: Damonte, Elsa Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; ArgentinaFil: Ghosh, Tuhin. University Of Burdwan; IndiaFil: Ray, Bimalendu. University Of Burdwan; IndiaElsevier Science2010-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/66379Mandal, Pinaki; Pujol, Carlos Alberto; Damonte, Elsa Beatriz; Ghosh, Tuhin; Ray, Bimalendu; Xylans from Scinaia hatei: Structural features, sulfation and anti-HSV activity; Elsevier Science; International Journal of Biological Macromolecules; 46; 2; 3-2010; 173-1780141-8130CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0141813009002669info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijbiomac.2009.12.003info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:22:53Zoai:ri.conicet.gov.ar:11336/66379instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:22:54.147CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Xylans from Scinaia hatei: Structural features, sulfation and anti-HSV activity
title Xylans from Scinaia hatei: Structural features, sulfation and anti-HSV activity
spellingShingle Xylans from Scinaia hatei: Structural features, sulfation and anti-HSV activity
Mandal, Pinaki
Antiviral Activity
Herpes Simplex Virus
Liagoraceae
Scinaia Hatei
Sulfation
Xylan
title_short Xylans from Scinaia hatei: Structural features, sulfation and anti-HSV activity
title_full Xylans from Scinaia hatei: Structural features, sulfation and anti-HSV activity
title_fullStr Xylans from Scinaia hatei: Structural features, sulfation and anti-HSV activity
title_full_unstemmed Xylans from Scinaia hatei: Structural features, sulfation and anti-HSV activity
title_sort Xylans from Scinaia hatei: Structural features, sulfation and anti-HSV activity
dc.creator.none.fl_str_mv Mandal, Pinaki
Pujol, Carlos Alberto
Damonte, Elsa Beatriz
Ghosh, Tuhin
Ray, Bimalendu
author Mandal, Pinaki
author_facet Mandal, Pinaki
Pujol, Carlos Alberto
Damonte, Elsa Beatriz
Ghosh, Tuhin
Ray, Bimalendu
author_role author
author2 Pujol, Carlos Alberto
Damonte, Elsa Beatriz
Ghosh, Tuhin
Ray, Bimalendu
author2_role author
author
author
author
dc.subject.none.fl_str_mv Antiviral Activity
Herpes Simplex Virus
Liagoraceae
Scinaia Hatei
Sulfation
Xylan
topic Antiviral Activity
Herpes Simplex Virus
Liagoraceae
Scinaia Hatei
Sulfation
Xylan
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Natural compounds offer interesting pharmacological perspectives for antiviral drug development with regard to broad-spectrum antiviral properties and novel modes of action. In this study, we have analyzed alkali-extracted xylan of Scinaia hatei. Alkali extraction of this red alga yielded a xylan shown to have a molecular mass of 120kDa and a linear structure of (1→4)-linked β-d-xylopyranosyl residues. Derivatives (S1, S2, S3 and S4) generated by chemical sulfation from this macromolecule had degree of sulfation between 0.93 and 1.95, and contained strong anti-HSV activity with inhibitory concentration 50% (IC50) from 0.22 to 1.37μg/ml. Furthermore, they had no direct inactivating effect on virions in a virucidal assay. Sulfate groups account for their in vitro antiviral activity. Interestingly, sulfated xylans already exerted anti-HSV activity when only pre-incubated with the cultured cells prior to infection, thus pointing to a main inhibitory effect on viral entry. © 2009 Elsevier B.V.
Fil: Mandal, Pinaki. University Of Burdwan; India
Fil: Pujol, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
Fil: Damonte, Elsa Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
Fil: Ghosh, Tuhin. University Of Burdwan; India
Fil: Ray, Bimalendu. University Of Burdwan; India
description Natural compounds offer interesting pharmacological perspectives for antiviral drug development with regard to broad-spectrum antiviral properties and novel modes of action. In this study, we have analyzed alkali-extracted xylan of Scinaia hatei. Alkali extraction of this red alga yielded a xylan shown to have a molecular mass of 120kDa and a linear structure of (1→4)-linked β-d-xylopyranosyl residues. Derivatives (S1, S2, S3 and S4) generated by chemical sulfation from this macromolecule had degree of sulfation between 0.93 and 1.95, and contained strong anti-HSV activity with inhibitory concentration 50% (IC50) from 0.22 to 1.37μg/ml. Furthermore, they had no direct inactivating effect on virions in a virucidal assay. Sulfate groups account for their in vitro antiviral activity. Interestingly, sulfated xylans already exerted anti-HSV activity when only pre-incubated with the cultured cells prior to infection, thus pointing to a main inhibitory effect on viral entry. © 2009 Elsevier B.V.
publishDate 2010
dc.date.none.fl_str_mv 2010-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/66379
Mandal, Pinaki; Pujol, Carlos Alberto; Damonte, Elsa Beatriz; Ghosh, Tuhin; Ray, Bimalendu; Xylans from Scinaia hatei: Structural features, sulfation and anti-HSV activity; Elsevier Science; International Journal of Biological Macromolecules; 46; 2; 3-2010; 173-178
0141-8130
CONICET Digital
CONICET
url http://hdl.handle.net/11336/66379
identifier_str_mv Mandal, Pinaki; Pujol, Carlos Alberto; Damonte, Elsa Beatriz; Ghosh, Tuhin; Ray, Bimalendu; Xylans from Scinaia hatei: Structural features, sulfation and anti-HSV activity; Elsevier Science; International Journal of Biological Macromolecules; 46; 2; 3-2010; 173-178
0141-8130
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0141813009002669
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijbiomac.2009.12.003
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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