Giantin mediates Golgi localization of Gal3-O-sulfotransferases and affects salivary mucin sulfation in patients with Sjögren’s disease

Autores
Nuñez, Matilde; Carvajal, Patricia; Aguilera, Sergio; Barrera Lier, María José; Matus, Soledad; Couto, Alicia Susana; Landoni, Malena; Boncompain, Gaelle; González, Sergio; Molina, Claudio; Pino, Karina; Indo, Sebastián; Figueroa, Lourdes; González, María Julieta; Castro, Isabel
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Sjögren’s disease is a chronic autoimmune disease characterized by symptoms of oral and oculardryness and extraglandular manifestations. Mouth dryness is not only due to reduced salivavolume, but also to alterations in the quality of salivary mucins in patients with Sjögren’s disease.Mucins play a leading role in mucosa hydration and protection, where sulfated and sialylatedoligosaccharides retain water molecules at the epithelial surface. The correct localization ofglycosyltransferases and sulfotransferases within the Golgi apparatus determines adequateO-glycosylation and sulfation of mucins, which depends on specific golgins that tether enzymebearing vesicles. Here, we show that a golgin called Giantin was mislocalized in salivary glandsfrom patients with Sjögren’s disease and formed protein complexes with Gal3-O-sulfotransferases(Gal3STs), which changed their localization in Giantin-knockout and -knockdown cells. Our resultssuggest that Giantin could tether Gal3ST-bearing vesicles and that its altered localization couldaffect Gal3ST activity, explaining the decreased sulfation of MUC5B observed in salivary glandsfrom patients with Sjögren’s disease.
Fil: Nuñez, Matilde. Universidad de Chile; Chile
Fil: Carvajal, Patricia. Universidad de Chile.; Chile
Fil: Aguilera, Sergio. Clínica INDISA; Chile
Fil: Barrera Lier, María José. Universidad San Sebastian.; Chile
Fil: Matus, Soledad. Universidad San Sebastian.; Chile
Fil: Couto, Alicia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Landoni, Malena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Boncompain, Gaelle. Sorbonne University; Francia
Fil: González, Sergio. Universidad Mayor.; Chile
Fil: Molina, Claudio. Universidad San Sebastian.; Chile
Fil: Pino, Karina. Universidad de Chile.; Chile
Fil: Indo, Sebastián. Universidad de Chile.; Chile
Fil: Figueroa, Lourdes. Universidad de Chile.; Chile
Fil: González, María Julieta. Universidad de Chile.; Chile
Fil: Castro, Isabel. Universidad de Chile.; Chile
Materia
Sjögren’s disease
Sulfation
Mucin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/262300

id CONICETDig_7f2c0d17378aa4e93fc2923e5fade9d8
oai_identifier_str oai:ri.conicet.gov.ar:11336/262300
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Giantin mediates Golgi localization of Gal3-O-sulfotransferases and affects salivary mucin sulfation in patients with Sjögren’s diseaseNuñez, MatildeCarvajal, PatriciaAguilera, SergioBarrera Lier, María JoséMatus, SoledadCouto, Alicia SusanaLandoni, MalenaBoncompain, GaelleGonzález, SergioMolina, ClaudioPino, KarinaIndo, SebastiánFigueroa, LourdesGonzález, María JulietaCastro, IsabelSjögren’s diseaseSulfationMucinhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Sjögren’s disease is a chronic autoimmune disease characterized by symptoms of oral and oculardryness and extraglandular manifestations. Mouth dryness is not only due to reduced salivavolume, but also to alterations in the quality of salivary mucins in patients with Sjögren’s disease.Mucins play a leading role in mucosa hydration and protection, where sulfated and sialylatedoligosaccharides retain water molecules at the epithelial surface. The correct localization ofglycosyltransferases and sulfotransferases within the Golgi apparatus determines adequateO-glycosylation and sulfation of mucins, which depends on specific golgins that tether enzymebearing vesicles. Here, we show that a golgin called Giantin was mislocalized in salivary glandsfrom patients with Sjögren’s disease and formed protein complexes with Gal3-O-sulfotransferases(Gal3STs), which changed their localization in Giantin-knockout and -knockdown cells. Our resultssuggest that Giantin could tether Gal3ST-bearing vesicles and that its altered localization couldaffect Gal3ST activity, explaining the decreased sulfation of MUC5B observed in salivary glandsfrom patients with Sjögren’s disease.Fil: Nuñez, Matilde. Universidad de Chile; ChileFil: Carvajal, Patricia. Universidad de Chile.; ChileFil: Aguilera, Sergio. Clínica INDISA; ChileFil: Barrera Lier, María José. Universidad San Sebastian.; ChileFil: Matus, Soledad. Universidad San Sebastian.; ChileFil: Couto, Alicia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaFil: Landoni, Malena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaFil: Boncompain, Gaelle. Sorbonne University; FranciaFil: González, Sergio. Universidad Mayor.; ChileFil: Molina, Claudio. Universidad San Sebastian.; ChileFil: Pino, Karina. Universidad de Chile.; ChileFil: Indo, Sebastián. Universidad de Chile.; ChileFil: Figueroa, Lourdes. Universidad de Chile.; ChileFil: González, María Julieta. Universidad de Chile.; ChileFil: Castro, Isabel. Universidad de Chile.; ChileAmerican Society for Clinical Investigation2024-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/262300Nuñez, Matilde; Carvajal, Patricia; Aguilera, Sergio; Barrera Lier, María José; Matus, Soledad; et al.; Giantin mediates Golgi localization of Gal3-O-sulfotransferases and affects salivary mucin sulfation in patients with Sjögren’s disease; American Society for Clinical Investigation; JCI Insight; 9; 22; 11-2024; 1-192379-3708CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://insight.jci.org/articles/view/171585info:eu-repo/semantics/altIdentifier/doi/10.1172/jci.insight.171585info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:55:28Zoai:ri.conicet.gov.ar:11336/262300instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:55:28.489CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Giantin mediates Golgi localization of Gal3-O-sulfotransferases and affects salivary mucin sulfation in patients with Sjögren’s disease
title Giantin mediates Golgi localization of Gal3-O-sulfotransferases and affects salivary mucin sulfation in patients with Sjögren’s disease
spellingShingle Giantin mediates Golgi localization of Gal3-O-sulfotransferases and affects salivary mucin sulfation in patients with Sjögren’s disease
Nuñez, Matilde
Sjögren’s disease
Sulfation
Mucin
title_short Giantin mediates Golgi localization of Gal3-O-sulfotransferases and affects salivary mucin sulfation in patients with Sjögren’s disease
title_full Giantin mediates Golgi localization of Gal3-O-sulfotransferases and affects salivary mucin sulfation in patients with Sjögren’s disease
title_fullStr Giantin mediates Golgi localization of Gal3-O-sulfotransferases and affects salivary mucin sulfation in patients with Sjögren’s disease
title_full_unstemmed Giantin mediates Golgi localization of Gal3-O-sulfotransferases and affects salivary mucin sulfation in patients with Sjögren’s disease
title_sort Giantin mediates Golgi localization of Gal3-O-sulfotransferases and affects salivary mucin sulfation in patients with Sjögren’s disease
dc.creator.none.fl_str_mv Nuñez, Matilde
Carvajal, Patricia
Aguilera, Sergio
Barrera Lier, María José
Matus, Soledad
Couto, Alicia Susana
Landoni, Malena
Boncompain, Gaelle
González, Sergio
Molina, Claudio
Pino, Karina
Indo, Sebastián
Figueroa, Lourdes
González, María Julieta
Castro, Isabel
author Nuñez, Matilde
author_facet Nuñez, Matilde
Carvajal, Patricia
Aguilera, Sergio
Barrera Lier, María José
Matus, Soledad
Couto, Alicia Susana
Landoni, Malena
Boncompain, Gaelle
González, Sergio
Molina, Claudio
Pino, Karina
Indo, Sebastián
Figueroa, Lourdes
González, María Julieta
Castro, Isabel
author_role author
author2 Carvajal, Patricia
Aguilera, Sergio
Barrera Lier, María José
Matus, Soledad
Couto, Alicia Susana
Landoni, Malena
Boncompain, Gaelle
González, Sergio
Molina, Claudio
Pino, Karina
Indo, Sebastián
Figueroa, Lourdes
González, María Julieta
Castro, Isabel
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Sjögren’s disease
Sulfation
Mucin
topic Sjögren’s disease
Sulfation
Mucin
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Sjögren’s disease is a chronic autoimmune disease characterized by symptoms of oral and oculardryness and extraglandular manifestations. Mouth dryness is not only due to reduced salivavolume, but also to alterations in the quality of salivary mucins in patients with Sjögren’s disease.Mucins play a leading role in mucosa hydration and protection, where sulfated and sialylatedoligosaccharides retain water molecules at the epithelial surface. The correct localization ofglycosyltransferases and sulfotransferases within the Golgi apparatus determines adequateO-glycosylation and sulfation of mucins, which depends on specific golgins that tether enzymebearing vesicles. Here, we show that a golgin called Giantin was mislocalized in salivary glandsfrom patients with Sjögren’s disease and formed protein complexes with Gal3-O-sulfotransferases(Gal3STs), which changed their localization in Giantin-knockout and -knockdown cells. Our resultssuggest that Giantin could tether Gal3ST-bearing vesicles and that its altered localization couldaffect Gal3ST activity, explaining the decreased sulfation of MUC5B observed in salivary glandsfrom patients with Sjögren’s disease.
Fil: Nuñez, Matilde. Universidad de Chile; Chile
Fil: Carvajal, Patricia. Universidad de Chile.; Chile
Fil: Aguilera, Sergio. Clínica INDISA; Chile
Fil: Barrera Lier, María José. Universidad San Sebastian.; Chile
Fil: Matus, Soledad. Universidad San Sebastian.; Chile
Fil: Couto, Alicia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Landoni, Malena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Boncompain, Gaelle. Sorbonne University; Francia
Fil: González, Sergio. Universidad Mayor.; Chile
Fil: Molina, Claudio. Universidad San Sebastian.; Chile
Fil: Pino, Karina. Universidad de Chile.; Chile
Fil: Indo, Sebastián. Universidad de Chile.; Chile
Fil: Figueroa, Lourdes. Universidad de Chile.; Chile
Fil: González, María Julieta. Universidad de Chile.; Chile
Fil: Castro, Isabel. Universidad de Chile.; Chile
description Sjögren’s disease is a chronic autoimmune disease characterized by symptoms of oral and oculardryness and extraglandular manifestations. Mouth dryness is not only due to reduced salivavolume, but also to alterations in the quality of salivary mucins in patients with Sjögren’s disease.Mucins play a leading role in mucosa hydration and protection, where sulfated and sialylatedoligosaccharides retain water molecules at the epithelial surface. The correct localization ofglycosyltransferases and sulfotransferases within the Golgi apparatus determines adequateO-glycosylation and sulfation of mucins, which depends on specific golgins that tether enzymebearing vesicles. Here, we show that a golgin called Giantin was mislocalized in salivary glandsfrom patients with Sjögren’s disease and formed protein complexes with Gal3-O-sulfotransferases(Gal3STs), which changed their localization in Giantin-knockout and -knockdown cells. Our resultssuggest that Giantin could tether Gal3ST-bearing vesicles and that its altered localization couldaffect Gal3ST activity, explaining the decreased sulfation of MUC5B observed in salivary glandsfrom patients with Sjögren’s disease.
publishDate 2024
dc.date.none.fl_str_mv 2024-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/262300
Nuñez, Matilde; Carvajal, Patricia; Aguilera, Sergio; Barrera Lier, María José; Matus, Soledad; et al.; Giantin mediates Golgi localization of Gal3-O-sulfotransferases and affects salivary mucin sulfation in patients with Sjögren’s disease; American Society for Clinical Investigation; JCI Insight; 9; 22; 11-2024; 1-19
2379-3708
CONICET Digital
CONICET
url http://hdl.handle.net/11336/262300
identifier_str_mv Nuñez, Matilde; Carvajal, Patricia; Aguilera, Sergio; Barrera Lier, María José; Matus, Soledad; et al.; Giantin mediates Golgi localization of Gal3-O-sulfotransferases and affects salivary mucin sulfation in patients with Sjögren’s disease; American Society for Clinical Investigation; JCI Insight; 9; 22; 11-2024; 1-19
2379-3708
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://insight.jci.org/articles/view/171585
info:eu-repo/semantics/altIdentifier/doi/10.1172/jci.insight.171585
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Clinical Investigation
publisher.none.fl_str_mv American Society for Clinical Investigation
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613672259813376
score 13.070432