Targeting of plasmodesmal proteins requires unconventional signals
- Autores
- Robles Luna, Gabriel; Li, Jiefu; Wang, Xu; Liao, Li; Jung-Youn Lee
- Año de publicación
- 2023
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Effective cellular signaling relies on precise spatial localization and dynamic interactions among proteins in specific subcellular compartments or niches, such as cell-to-cell contact sites and junctions. In plants, endogenous and pathogenic proteins gained the ability to target plasmodesmata, membrane-lined cytoplasmic connections, through evolution to regulate or exploit cellular signaling across cell wall boundaries. For example, the receptor-like membrane protein PLASMODESMATA-LOCATED PROTEIN 5 (PDLP5), a potent regulator of plasmodesmal permeability, generates feed-forward or feed-back signals important for plant immunity and root development. However, the molecular features that determine the plasmodesmal association of PDLP5 or other proteins remain largely unknown, and no protein motifs have been identified as plasmodesmal targeting signals. Here, we developed an approach combining custom-built machine-learning algorithms and targeted mutagenesis to examine PDLP5 in Arabidopsis thaliana and Nicotiana benthamiana. We report that PDLP5 and its closely related proteins carry unconventional targeting signals consisting of short stretches of amino acids. PDLP5 contains two divergent, tandemly arranged signals, either of which is sufficient for localization and biological function in regulating viral movement through plasmodesmata. Notably, plasmodesmal targeting signals exhibit little sequence conservation but are located similarly proximal to the membrane. These features appear to be a common theme in plasmodesmal targeting.
Fil: Robles Luna, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Li, Jiefu. University of Delaware; Estados Unidos
Fil: Wang, Xu. University of Delaware; Estados Unidos
Fil: Liao, Li. University of Delaware; Estados Unidos
Fil: Jung-Youn Lee. University of Delaware; Estados Unidos - Materia
-
Plasmodesmata
Targeting signal
virus movement
Machine learning - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/250443
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Targeting of plasmodesmal proteins requires unconventional signalsRobles Luna, GabrielLi, JiefuWang, XuLiao, LiJung-Youn LeePlasmodesmataTargeting signalvirus movementMachine learninghttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Effective cellular signaling relies on precise spatial localization and dynamic interactions among proteins in specific subcellular compartments or niches, such as cell-to-cell contact sites and junctions. In plants, endogenous and pathogenic proteins gained the ability to target plasmodesmata, membrane-lined cytoplasmic connections, through evolution to regulate or exploit cellular signaling across cell wall boundaries. For example, the receptor-like membrane protein PLASMODESMATA-LOCATED PROTEIN 5 (PDLP5), a potent regulator of plasmodesmal permeability, generates feed-forward or feed-back signals important for plant immunity and root development. However, the molecular features that determine the plasmodesmal association of PDLP5 or other proteins remain largely unknown, and no protein motifs have been identified as plasmodesmal targeting signals. Here, we developed an approach combining custom-built machine-learning algorithms and targeted mutagenesis to examine PDLP5 in Arabidopsis thaliana and Nicotiana benthamiana. We report that PDLP5 and its closely related proteins carry unconventional targeting signals consisting of short stretches of amino acids. PDLP5 contains two divergent, tandemly arranged signals, either of which is sufficient for localization and biological function in regulating viral movement through plasmodesmata. Notably, plasmodesmal targeting signals exhibit little sequence conservation but are located similarly proximal to the membrane. These features appear to be a common theme in plasmodesmal targeting.Fil: Robles Luna, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Li, Jiefu. University of Delaware; Estados UnidosFil: Wang, Xu. University of Delaware; Estados UnidosFil: Liao, Li. University of Delaware; Estados UnidosFil: Jung-Youn Lee. University of Delaware; Estados UnidosAmerican Society of Plant Biologist2023-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/250443Robles Luna, Gabriel; Li, Jiefu; Wang, Xu; Liao, Li; Jung-Youn Lee; Targeting of plasmodesmal proteins requires unconventional signals; American Society of Plant Biologist; Plant Cell; 35; 8; 5-2023; 3035-30521040-4651CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/plcell/advance-article/doi/10.1093/plcell/koad152/7178006?utm_source=authortollfreelink&utm_campaign=plcell&utm_medium=email&guestAccessKey=5044d9bd-2522-4a48-a918-8d5c6141418binfo:eu-repo/semantics/altIdentifier/doi/10.1093/plcell/koad152info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:53:52Zoai:ri.conicet.gov.ar:11336/250443instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:53:53.225CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Targeting of plasmodesmal proteins requires unconventional signals |
title |
Targeting of plasmodesmal proteins requires unconventional signals |
spellingShingle |
Targeting of plasmodesmal proteins requires unconventional signals Robles Luna, Gabriel Plasmodesmata Targeting signal virus movement Machine learning |
title_short |
Targeting of plasmodesmal proteins requires unconventional signals |
title_full |
Targeting of plasmodesmal proteins requires unconventional signals |
title_fullStr |
Targeting of plasmodesmal proteins requires unconventional signals |
title_full_unstemmed |
Targeting of plasmodesmal proteins requires unconventional signals |
title_sort |
Targeting of plasmodesmal proteins requires unconventional signals |
dc.creator.none.fl_str_mv |
Robles Luna, Gabriel Li, Jiefu Wang, Xu Liao, Li Jung-Youn Lee |
author |
Robles Luna, Gabriel |
author_facet |
Robles Luna, Gabriel Li, Jiefu Wang, Xu Liao, Li Jung-Youn Lee |
author_role |
author |
author2 |
Li, Jiefu Wang, Xu Liao, Li Jung-Youn Lee |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Plasmodesmata Targeting signal virus movement Machine learning |
topic |
Plasmodesmata Targeting signal virus movement Machine learning |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Effective cellular signaling relies on precise spatial localization and dynamic interactions among proteins in specific subcellular compartments or niches, such as cell-to-cell contact sites and junctions. In plants, endogenous and pathogenic proteins gained the ability to target plasmodesmata, membrane-lined cytoplasmic connections, through evolution to regulate or exploit cellular signaling across cell wall boundaries. For example, the receptor-like membrane protein PLASMODESMATA-LOCATED PROTEIN 5 (PDLP5), a potent regulator of plasmodesmal permeability, generates feed-forward or feed-back signals important for plant immunity and root development. However, the molecular features that determine the plasmodesmal association of PDLP5 or other proteins remain largely unknown, and no protein motifs have been identified as plasmodesmal targeting signals. Here, we developed an approach combining custom-built machine-learning algorithms and targeted mutagenesis to examine PDLP5 in Arabidopsis thaliana and Nicotiana benthamiana. We report that PDLP5 and its closely related proteins carry unconventional targeting signals consisting of short stretches of amino acids. PDLP5 contains two divergent, tandemly arranged signals, either of which is sufficient for localization and biological function in regulating viral movement through plasmodesmata. Notably, plasmodesmal targeting signals exhibit little sequence conservation but are located similarly proximal to the membrane. These features appear to be a common theme in plasmodesmal targeting. Fil: Robles Luna, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Li, Jiefu. University of Delaware; Estados Unidos Fil: Wang, Xu. University of Delaware; Estados Unidos Fil: Liao, Li. University of Delaware; Estados Unidos Fil: Jung-Youn Lee. University of Delaware; Estados Unidos |
description |
Effective cellular signaling relies on precise spatial localization and dynamic interactions among proteins in specific subcellular compartments or niches, such as cell-to-cell contact sites and junctions. In plants, endogenous and pathogenic proteins gained the ability to target plasmodesmata, membrane-lined cytoplasmic connections, through evolution to regulate or exploit cellular signaling across cell wall boundaries. For example, the receptor-like membrane protein PLASMODESMATA-LOCATED PROTEIN 5 (PDLP5), a potent regulator of plasmodesmal permeability, generates feed-forward or feed-back signals important for plant immunity and root development. However, the molecular features that determine the plasmodesmal association of PDLP5 or other proteins remain largely unknown, and no protein motifs have been identified as plasmodesmal targeting signals. Here, we developed an approach combining custom-built machine-learning algorithms and targeted mutagenesis to examine PDLP5 in Arabidopsis thaliana and Nicotiana benthamiana. We report that PDLP5 and its closely related proteins carry unconventional targeting signals consisting of short stretches of amino acids. PDLP5 contains two divergent, tandemly arranged signals, either of which is sufficient for localization and biological function in regulating viral movement through plasmodesmata. Notably, plasmodesmal targeting signals exhibit little sequence conservation but are located similarly proximal to the membrane. These features appear to be a common theme in plasmodesmal targeting. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-05 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/250443 Robles Luna, Gabriel; Li, Jiefu; Wang, Xu; Liao, Li; Jung-Youn Lee; Targeting of plasmodesmal proteins requires unconventional signals; American Society of Plant Biologist; Plant Cell; 35; 8; 5-2023; 3035-3052 1040-4651 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/250443 |
identifier_str_mv |
Robles Luna, Gabriel; Li, Jiefu; Wang, Xu; Liao, Li; Jung-Youn Lee; Targeting of plasmodesmal proteins requires unconventional signals; American Society of Plant Biologist; Plant Cell; 35; 8; 5-2023; 3035-3052 1040-4651 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/plcell/advance-article/doi/10.1093/plcell/koad152/7178006?utm_source=authortollfreelink&utm_campaign=plcell&utm_medium=email&guestAccessKey=5044d9bd-2522-4a48-a918-8d5c6141418b info:eu-repo/semantics/altIdentifier/doi/10.1093/plcell/koad152 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
American Society of Plant Biologist |
publisher.none.fl_str_mv |
American Society of Plant Biologist |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |