Bioaccessibility of folic acid in egg white nanocarriers and protein digestion profile in solution and in emulsion

Autores
Arzeni, Carolina; Pilosof, Ana Maria Renata
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The objective of this work is to assess bioaccessibility of folic acid in egg white (EW) nanocarriers after digestion of solutions and emulsions containing the vitamin-protein complexes. Furthermore, the influence of folic acid binding on the peptides profile after digestion is also evaluated. To this end an in vitro gastrointestinal digestion was performed and the pattern of digestion products was determined by high performance liquid chromatography. When folic acid was bound to nanocarriers, the vitamin peak area was approximately 80% lower than the one for folic acid alone. In emulsion, even less amount was detected. After digestion, no significant differences on folic acid peak areas were observed. Folic acid was released from nanocarriers after digestion, thus indicating that the vitamin would be bioaccessible in the site of absorption. Moreover, it was demonstrated by tricine-SDS-PAGE that folic acid binding induced a slight aggregation of proteins, although it did not alter the profile of peptides obtained after digestion. Binding of folic acid to nanocarriers proved to cause no negative effect on EW proteins digestibility. On the contrary, it seemed to slightly enhance their digestion. These nanocarriers would constitute an adequate system for the preservation, transport and target delivery of this bioactive.
Fil: Arzeni, Carolina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias. Instituto de Tecnología de Alimentos y Procesos Químicos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Tecnología de Alimentos y Procesos Químicos; Argentina
Fil: Pilosof, Ana Maria Renata. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias. Instituto de Tecnología de Alimentos y Procesos Químicos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Tecnología de Alimentos y Procesos Químicos; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina
Materia
BIOACCESSIBILITY
EGG WHITE
FOLIC ACID
NANOCARRIERS
PEPTIDES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/147385
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Bioaccessibility of folic acid in egg white nanocarriers and protein digestion profile in solution and in emulsionArzeni, CarolinaPilosof, Ana Maria RenataBIOACCESSIBILITYEGG WHITEFOLIC ACIDNANOCARRIERSPEPTIDEShttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2The objective of this work is to assess bioaccessibility of folic acid in egg white (EW) nanocarriers after digestion of solutions and emulsions containing the vitamin-protein complexes. Furthermore, the influence of folic acid binding on the peptides profile after digestion is also evaluated. To this end an in vitro gastrointestinal digestion was performed and the pattern of digestion products was determined by high performance liquid chromatography. When folic acid was bound to nanocarriers, the vitamin peak area was approximately 80% lower than the one for folic acid alone. In emulsion, even less amount was detected. After digestion, no significant differences on folic acid peak areas were observed. Folic acid was released from nanocarriers after digestion, thus indicating that the vitamin would be bioaccessible in the site of absorption. Moreover, it was demonstrated by tricine-SDS-PAGE that folic acid binding induced a slight aggregation of proteins, although it did not alter the profile of peptides obtained after digestion. Binding of folic acid to nanocarriers proved to cause no negative effect on EW proteins digestibility. On the contrary, it seemed to slightly enhance their digestion. These nanocarriers would constitute an adequate system for the preservation, transport and target delivery of this bioactive.Fil: Arzeni, Carolina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias. Instituto de Tecnología de Alimentos y Procesos Químicos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Tecnología de Alimentos y Procesos Químicos; ArgentinaFil: Pilosof, Ana Maria Renata. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias. Instituto de Tecnología de Alimentos y Procesos Químicos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Tecnología de Alimentos y Procesos Químicos; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; ArgentinaElsevier Science2019-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/147385Arzeni, Carolina; Pilosof, Ana Maria Renata; Bioaccessibility of folic acid in egg white nanocarriers and protein digestion profile in solution and in emulsion; Elsevier Science; LWT - Food Science and Technology; 111; 8-2019; 470-4770023-6438CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.lwt.2019.05.070info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0023643819304955info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:07:34Zoai:ri.conicet.gov.ar:11336/147385instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:07:34.522CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bioaccessibility of folic acid in egg white nanocarriers and protein digestion profile in solution and in emulsion
title Bioaccessibility of folic acid in egg white nanocarriers and protein digestion profile in solution and in emulsion
spellingShingle Bioaccessibility of folic acid in egg white nanocarriers and protein digestion profile in solution and in emulsion
Arzeni, Carolina
BIOACCESSIBILITY
EGG WHITE
FOLIC ACID
NANOCARRIERS
PEPTIDES
title_short Bioaccessibility of folic acid in egg white nanocarriers and protein digestion profile in solution and in emulsion
title_full Bioaccessibility of folic acid in egg white nanocarriers and protein digestion profile in solution and in emulsion
title_fullStr Bioaccessibility of folic acid in egg white nanocarriers and protein digestion profile in solution and in emulsion
title_full_unstemmed Bioaccessibility of folic acid in egg white nanocarriers and protein digestion profile in solution and in emulsion
title_sort Bioaccessibility of folic acid in egg white nanocarriers and protein digestion profile in solution and in emulsion
dc.creator.none.fl_str_mv Arzeni, Carolina
Pilosof, Ana Maria Renata
author Arzeni, Carolina
author_facet Arzeni, Carolina
Pilosof, Ana Maria Renata
author_role author
author2 Pilosof, Ana Maria Renata
author2_role author
dc.subject.none.fl_str_mv BIOACCESSIBILITY
EGG WHITE
FOLIC ACID
NANOCARRIERS
PEPTIDES
topic BIOACCESSIBILITY
EGG WHITE
FOLIC ACID
NANOCARRIERS
PEPTIDES
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The objective of this work is to assess bioaccessibility of folic acid in egg white (EW) nanocarriers after digestion of solutions and emulsions containing the vitamin-protein complexes. Furthermore, the influence of folic acid binding on the peptides profile after digestion is also evaluated. To this end an in vitro gastrointestinal digestion was performed and the pattern of digestion products was determined by high performance liquid chromatography. When folic acid was bound to nanocarriers, the vitamin peak area was approximately 80% lower than the one for folic acid alone. In emulsion, even less amount was detected. After digestion, no significant differences on folic acid peak areas were observed. Folic acid was released from nanocarriers after digestion, thus indicating that the vitamin would be bioaccessible in the site of absorption. Moreover, it was demonstrated by tricine-SDS-PAGE that folic acid binding induced a slight aggregation of proteins, although it did not alter the profile of peptides obtained after digestion. Binding of folic acid to nanocarriers proved to cause no negative effect on EW proteins digestibility. On the contrary, it seemed to slightly enhance their digestion. These nanocarriers would constitute an adequate system for the preservation, transport and target delivery of this bioactive.
Fil: Arzeni, Carolina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias. Instituto de Tecnología de Alimentos y Procesos Químicos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Tecnología de Alimentos y Procesos Químicos; Argentina
Fil: Pilosof, Ana Maria Renata. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias. Instituto de Tecnología de Alimentos y Procesos Químicos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Tecnología de Alimentos y Procesos Químicos; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina
description The objective of this work is to assess bioaccessibility of folic acid in egg white (EW) nanocarriers after digestion of solutions and emulsions containing the vitamin-protein complexes. Furthermore, the influence of folic acid binding on the peptides profile after digestion is also evaluated. To this end an in vitro gastrointestinal digestion was performed and the pattern of digestion products was determined by high performance liquid chromatography. When folic acid was bound to nanocarriers, the vitamin peak area was approximately 80% lower than the one for folic acid alone. In emulsion, even less amount was detected. After digestion, no significant differences on folic acid peak areas were observed. Folic acid was released from nanocarriers after digestion, thus indicating that the vitamin would be bioaccessible in the site of absorption. Moreover, it was demonstrated by tricine-SDS-PAGE that folic acid binding induced a slight aggregation of proteins, although it did not alter the profile of peptides obtained after digestion. Binding of folic acid to nanocarriers proved to cause no negative effect on EW proteins digestibility. On the contrary, it seemed to slightly enhance their digestion. These nanocarriers would constitute an adequate system for the preservation, transport and target delivery of this bioactive.
publishDate 2019
dc.date.none.fl_str_mv 2019-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/147385
Arzeni, Carolina; Pilosof, Ana Maria Renata; Bioaccessibility of folic acid in egg white nanocarriers and protein digestion profile in solution and in emulsion; Elsevier Science; LWT - Food Science and Technology; 111; 8-2019; 470-477
0023-6438
CONICET Digital
CONICET
url http://hdl.handle.net/11336/147385
identifier_str_mv Arzeni, Carolina; Pilosof, Ana Maria Renata; Bioaccessibility of folic acid in egg white nanocarriers and protein digestion profile in solution and in emulsion; Elsevier Science; LWT - Food Science and Technology; 111; 8-2019; 470-477
0023-6438
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.lwt.2019.05.070
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0023643819304955
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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