Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cell from BLV-infected catlle

Autores
Dolcini, Guillermina Laura; Ladera Gomez, Marla Eliana; Nieto Farías, María Victoria; Vater, Adrian Alejandro; Ceriani, Maria Carolina
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The bovine leukemia virus (BLV) is an exogenous retrovirus belonging to the Deltaretrovirus genus. This enveloped virus naturally infects B-lymphocytes causing enzootic bovine leukemia (EBL), which is spread worldwide, mainly in dairy cattle. The virus might produce cell-mediated immunity disorders, which may lead to opportunistic infections in BLV-infected cattle. Thus, the performance of dairy cattle is impaired, leading to large economic losses in the dairy industry. A study was carried out to investigate the levels of proviral load as well as mRNA expression of cytokines in peripheral blood mononuclear cells (PBMC) and milk somatic cells (SC) of LPL, HPL and uninfected cattle (controls). A total of 51 BLV-infected cows and 16 controls were included. The levels of proviral load in PBMC and SC as well as the expression levels of cytokine mRNA between LPL and HPL cattle were measured by real-time PCR (qPCR). LPL was detected in milk SC from BLV-infected animals. Three cytokines, IFN-γ, IL-12 and IL-6, were found to be up-regulated in the LPL group, and one cytokine, IL-10, was found to be down-regulated in PBMC. The expression levels of IL-12 mRNA in SC from LPL animals were higher than in the HPL group, while IFN-γ, IL-10 and IL-6 mRNA expression levels were significantly down-regulated. The results showed differences in cytokine expression in both PBMC and SC between LPL and HPL cattle. This is the first study to provide a functional phenotype of immune status by establishing a cytokine profile in milk SC from LPL, HPL, and BLV-negative cattle.
Fil: Dolcini, Guillermina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Ladera Gomez, Marla Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Nieto Farías, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Vater, Adrian Alejandro. Escuela de Educación Secundaria Agraria Nº1 Dr. Ramón Santamarina; Argentina
Fil: Ceriani, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
I Congreso Argentino Microbiologia Veterinaria
La Plata
Argentina
Asociacion Argentina Microbiologia
Materia
LEUCOSIS BOVINA
CARGA PROVIRAL
CITOQUINAS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/170538

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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cell from BLV-infected catlleDolcini, Guillermina LauraLadera Gomez, Marla ElianaNieto Farías, María VictoriaVater, Adrian AlejandroCeriani, Maria CarolinaLEUCOSIS BOVINACARGA PROVIRALCITOQUINAShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The bovine leukemia virus (BLV) is an exogenous retrovirus belonging to the Deltaretrovirus genus. This enveloped virus naturally infects B-lymphocytes causing enzootic bovine leukemia (EBL), which is spread worldwide, mainly in dairy cattle. The virus might produce cell-mediated immunity disorders, which may lead to opportunistic infections in BLV-infected cattle. Thus, the performance of dairy cattle is impaired, leading to large economic losses in the dairy industry. A study was carried out to investigate the levels of proviral load as well as mRNA expression of cytokines in peripheral blood mononuclear cells (PBMC) and milk somatic cells (SC) of LPL, HPL and uninfected cattle (controls). A total of 51 BLV-infected cows and 16 controls were included. The levels of proviral load in PBMC and SC as well as the expression levels of cytokine mRNA between LPL and HPL cattle were measured by real-time PCR (qPCR). LPL was detected in milk SC from BLV-infected animals. Three cytokines, IFN-γ, IL-12 and IL-6, were found to be up-regulated in the LPL group, and one cytokine, IL-10, was found to be down-regulated in PBMC. The expression levels of IL-12 mRNA in SC from LPL animals were higher than in the HPL group, while IFN-γ, IL-10 and IL-6 mRNA expression levels were significantly down-regulated. The results showed differences in cytokine expression in both PBMC and SC between LPL and HPL cattle. This is the first study to provide a functional phenotype of immune status by establishing a cytokine profile in milk SC from LPL, HPL, and BLV-negative cattle.Fil: Dolcini, Guillermina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Ladera Gomez, Marla Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Nieto Farías, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Vater, Adrian Alejandro. Escuela de Educación Secundaria Agraria Nº1 Dr. Ramón Santamarina; ArgentinaFil: Ceriani, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaI Congreso Argentino Microbiologia VeterinariaLa PlataArgentinaAsociacion Argentina MicrobiologiaUniversidad Nacional De La Plata. Facultad De Ciencias Veterinaria2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/170538Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cell from BLV-infected catlle; I Congreso Argentino Microbiologia Veterinaria; La Plata; Argentina; 2021; 315-316978-950-34-2018-8CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://congresos.unlp.edu.ar/microvet/wp-content/uploads/sites/19/2021/08/LIBRO-DE-RESUMENES-I-CMV-2021.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:12:34Zoai:ri.conicet.gov.ar:11336/170538instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:12:34.314CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cell from BLV-infected catlle
title Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cell from BLV-infected catlle
spellingShingle Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cell from BLV-infected catlle
Dolcini, Guillermina Laura
LEUCOSIS BOVINA
CARGA PROVIRAL
CITOQUINAS
title_short Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cell from BLV-infected catlle
title_full Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cell from BLV-infected catlle
title_fullStr Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cell from BLV-infected catlle
title_full_unstemmed Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cell from BLV-infected catlle
title_sort Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cell from BLV-infected catlle
dc.creator.none.fl_str_mv Dolcini, Guillermina Laura
Ladera Gomez, Marla Eliana
Nieto Farías, María Victoria
Vater, Adrian Alejandro
Ceriani, Maria Carolina
author Dolcini, Guillermina Laura
author_facet Dolcini, Guillermina Laura
Ladera Gomez, Marla Eliana
Nieto Farías, María Victoria
Vater, Adrian Alejandro
Ceriani, Maria Carolina
author_role author
author2 Ladera Gomez, Marla Eliana
Nieto Farías, María Victoria
Vater, Adrian Alejandro
Ceriani, Maria Carolina
author2_role author
author
author
author
dc.subject.none.fl_str_mv LEUCOSIS BOVINA
CARGA PROVIRAL
CITOQUINAS
topic LEUCOSIS BOVINA
CARGA PROVIRAL
CITOQUINAS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The bovine leukemia virus (BLV) is an exogenous retrovirus belonging to the Deltaretrovirus genus. This enveloped virus naturally infects B-lymphocytes causing enzootic bovine leukemia (EBL), which is spread worldwide, mainly in dairy cattle. The virus might produce cell-mediated immunity disorders, which may lead to opportunistic infections in BLV-infected cattle. Thus, the performance of dairy cattle is impaired, leading to large economic losses in the dairy industry. A study was carried out to investigate the levels of proviral load as well as mRNA expression of cytokines in peripheral blood mononuclear cells (PBMC) and milk somatic cells (SC) of LPL, HPL and uninfected cattle (controls). A total of 51 BLV-infected cows and 16 controls were included. The levels of proviral load in PBMC and SC as well as the expression levels of cytokine mRNA between LPL and HPL cattle were measured by real-time PCR (qPCR). LPL was detected in milk SC from BLV-infected animals. Three cytokines, IFN-γ, IL-12 and IL-6, were found to be up-regulated in the LPL group, and one cytokine, IL-10, was found to be down-regulated in PBMC. The expression levels of IL-12 mRNA in SC from LPL animals were higher than in the HPL group, while IFN-γ, IL-10 and IL-6 mRNA expression levels were significantly down-regulated. The results showed differences in cytokine expression in both PBMC and SC between LPL and HPL cattle. This is the first study to provide a functional phenotype of immune status by establishing a cytokine profile in milk SC from LPL, HPL, and BLV-negative cattle.
Fil: Dolcini, Guillermina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Ladera Gomez, Marla Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Nieto Farías, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Vater, Adrian Alejandro. Escuela de Educación Secundaria Agraria Nº1 Dr. Ramón Santamarina; Argentina
Fil: Ceriani, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
I Congreso Argentino Microbiologia Veterinaria
La Plata
Argentina
Asociacion Argentina Microbiologia
description The bovine leukemia virus (BLV) is an exogenous retrovirus belonging to the Deltaretrovirus genus. This enveloped virus naturally infects B-lymphocytes causing enzootic bovine leukemia (EBL), which is spread worldwide, mainly in dairy cattle. The virus might produce cell-mediated immunity disorders, which may lead to opportunistic infections in BLV-infected cattle. Thus, the performance of dairy cattle is impaired, leading to large economic losses in the dairy industry. A study was carried out to investigate the levels of proviral load as well as mRNA expression of cytokines in peripheral blood mononuclear cells (PBMC) and milk somatic cells (SC) of LPL, HPL and uninfected cattle (controls). A total of 51 BLV-infected cows and 16 controls were included. The levels of proviral load in PBMC and SC as well as the expression levels of cytokine mRNA between LPL and HPL cattle were measured by real-time PCR (qPCR). LPL was detected in milk SC from BLV-infected animals. Three cytokines, IFN-γ, IL-12 and IL-6, were found to be up-regulated in the LPL group, and one cytokine, IL-10, was found to be down-regulated in PBMC. The expression levels of IL-12 mRNA in SC from LPL animals were higher than in the HPL group, while IFN-γ, IL-10 and IL-6 mRNA expression levels were significantly down-regulated. The results showed differences in cytokine expression in both PBMC and SC between LPL and HPL cattle. This is the first study to provide a functional phenotype of immune status by establishing a cytokine profile in milk SC from LPL, HPL, and BLV-negative cattle.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/170538
Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cell from BLV-infected catlle; I Congreso Argentino Microbiologia Veterinaria; La Plata; Argentina; 2021; 315-316
978-950-34-2018-8
CONICET Digital
CONICET
url http://hdl.handle.net/11336/170538
identifier_str_mv Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cell from BLV-infected catlle; I Congreso Argentino Microbiologia Veterinaria; La Plata; Argentina; 2021; 315-316
978-950-34-2018-8
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://congresos.unlp.edu.ar/microvet/wp-content/uploads/sites/19/2021/08/LIBRO-DE-RESUMENES-I-CMV-2021.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Universidad Nacional De La Plata. Facultad De Ciencias Veterinaria
publisher.none.fl_str_mv Universidad Nacional De La Plata. Facultad De Ciencias Veterinaria
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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