Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cell from BLV-infected catlle
- Autores
- Dolcini, Guillermina Laura; Ladera Gomez, Marla Eliana; Nieto Farías, María Victoria; Vater, Adrian Alejandro; Ceriani, Maria Carolina
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- The bovine leukemia virus (BLV) is an exogenous retrovirus belonging to the Deltaretrovirus genus. This enveloped virus naturally infects B-lymphocytes causing enzootic bovine leukemia (EBL), which is spread worldwide, mainly in dairy cattle. The virus might produce cell-mediated immunity disorders, which may lead to opportunistic infections in BLV-infected cattle. Thus, the performance of dairy cattle is impaired, leading to large economic losses in the dairy industry. A study was carried out to investigate the levels of proviral load as well as mRNA expression of cytokines in peripheral blood mononuclear cells (PBMC) and milk somatic cells (SC) of LPL, HPL and uninfected cattle (controls). A total of 51 BLV-infected cows and 16 controls were included. The levels of proviral load in PBMC and SC as well as the expression levels of cytokine mRNA between LPL and HPL cattle were measured by real-time PCR (qPCR). LPL was detected in milk SC from BLV-infected animals. Three cytokines, IFN-γ, IL-12 and IL-6, were found to be up-regulated in the LPL group, and one cytokine, IL-10, was found to be down-regulated in PBMC. The expression levels of IL-12 mRNA in SC from LPL animals were higher than in the HPL group, while IFN-γ, IL-10 and IL-6 mRNA expression levels were significantly down-regulated. The results showed differences in cytokine expression in both PBMC and SC between LPL and HPL cattle. This is the first study to provide a functional phenotype of immune status by establishing a cytokine profile in milk SC from LPL, HPL, and BLV-negative cattle.
Fil: Dolcini, Guillermina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Ladera Gomez, Marla Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Nieto Farías, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Vater, Adrian Alejandro. Escuela de Educación Secundaria Agraria Nº1 Dr. Ramón Santamarina; Argentina
Fil: Ceriani, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
I Congreso Argentino Microbiologia Veterinaria
La Plata
Argentina
Asociacion Argentina Microbiologia - Materia
-
LEUCOSIS BOVINA
CARGA PROVIRAL
CITOQUINAS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/170538
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Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cell from BLV-infected catlleDolcini, Guillermina LauraLadera Gomez, Marla ElianaNieto Farías, María VictoriaVater, Adrian AlejandroCeriani, Maria CarolinaLEUCOSIS BOVINACARGA PROVIRALCITOQUINAShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The bovine leukemia virus (BLV) is an exogenous retrovirus belonging to the Deltaretrovirus genus. This enveloped virus naturally infects B-lymphocytes causing enzootic bovine leukemia (EBL), which is spread worldwide, mainly in dairy cattle. The virus might produce cell-mediated immunity disorders, which may lead to opportunistic infections in BLV-infected cattle. Thus, the performance of dairy cattle is impaired, leading to large economic losses in the dairy industry. A study was carried out to investigate the levels of proviral load as well as mRNA expression of cytokines in peripheral blood mononuclear cells (PBMC) and milk somatic cells (SC) of LPL, HPL and uninfected cattle (controls). A total of 51 BLV-infected cows and 16 controls were included. The levels of proviral load in PBMC and SC as well as the expression levels of cytokine mRNA between LPL and HPL cattle were measured by real-time PCR (qPCR). LPL was detected in milk SC from BLV-infected animals. Three cytokines, IFN-γ, IL-12 and IL-6, were found to be up-regulated in the LPL group, and one cytokine, IL-10, was found to be down-regulated in PBMC. The expression levels of IL-12 mRNA in SC from LPL animals were higher than in the HPL group, while IFN-γ, IL-10 and IL-6 mRNA expression levels were significantly down-regulated. The results showed differences in cytokine expression in both PBMC and SC between LPL and HPL cattle. This is the first study to provide a functional phenotype of immune status by establishing a cytokine profile in milk SC from LPL, HPL, and BLV-negative cattle.Fil: Dolcini, Guillermina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Ladera Gomez, Marla Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Nieto Farías, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Vater, Adrian Alejandro. Escuela de Educación Secundaria Agraria Nº1 Dr. Ramón Santamarina; ArgentinaFil: Ceriani, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaI Congreso Argentino Microbiologia VeterinariaLa PlataArgentinaAsociacion Argentina MicrobiologiaUniversidad Nacional De La Plata. Facultad De Ciencias Veterinaria2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/170538Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cell from BLV-infected catlle; I Congreso Argentino Microbiologia Veterinaria; La Plata; Argentina; 2021; 315-316978-950-34-2018-8CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://congresos.unlp.edu.ar/microvet/wp-content/uploads/sites/19/2021/08/LIBRO-DE-RESUMENES-I-CMV-2021.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:12:34Zoai:ri.conicet.gov.ar:11336/170538instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:12:34.314CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cell from BLV-infected catlle |
title |
Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cell from BLV-infected catlle |
spellingShingle |
Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cell from BLV-infected catlle Dolcini, Guillermina Laura LEUCOSIS BOVINA CARGA PROVIRAL CITOQUINAS |
title_short |
Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cell from BLV-infected catlle |
title_full |
Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cell from BLV-infected catlle |
title_fullStr |
Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cell from BLV-infected catlle |
title_full_unstemmed |
Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cell from BLV-infected catlle |
title_sort |
Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cell from BLV-infected catlle |
dc.creator.none.fl_str_mv |
Dolcini, Guillermina Laura Ladera Gomez, Marla Eliana Nieto Farías, María Victoria Vater, Adrian Alejandro Ceriani, Maria Carolina |
author |
Dolcini, Guillermina Laura |
author_facet |
Dolcini, Guillermina Laura Ladera Gomez, Marla Eliana Nieto Farías, María Victoria Vater, Adrian Alejandro Ceriani, Maria Carolina |
author_role |
author |
author2 |
Ladera Gomez, Marla Eliana Nieto Farías, María Victoria Vater, Adrian Alejandro Ceriani, Maria Carolina |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
LEUCOSIS BOVINA CARGA PROVIRAL CITOQUINAS |
topic |
LEUCOSIS BOVINA CARGA PROVIRAL CITOQUINAS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The bovine leukemia virus (BLV) is an exogenous retrovirus belonging to the Deltaretrovirus genus. This enveloped virus naturally infects B-lymphocytes causing enzootic bovine leukemia (EBL), which is spread worldwide, mainly in dairy cattle. The virus might produce cell-mediated immunity disorders, which may lead to opportunistic infections in BLV-infected cattle. Thus, the performance of dairy cattle is impaired, leading to large economic losses in the dairy industry. A study was carried out to investigate the levels of proviral load as well as mRNA expression of cytokines in peripheral blood mononuclear cells (PBMC) and milk somatic cells (SC) of LPL, HPL and uninfected cattle (controls). A total of 51 BLV-infected cows and 16 controls were included. The levels of proviral load in PBMC and SC as well as the expression levels of cytokine mRNA between LPL and HPL cattle were measured by real-time PCR (qPCR). LPL was detected in milk SC from BLV-infected animals. Three cytokines, IFN-γ, IL-12 and IL-6, were found to be up-regulated in the LPL group, and one cytokine, IL-10, was found to be down-regulated in PBMC. The expression levels of IL-12 mRNA in SC from LPL animals were higher than in the HPL group, while IFN-γ, IL-10 and IL-6 mRNA expression levels were significantly down-regulated. The results showed differences in cytokine expression in both PBMC and SC between LPL and HPL cattle. This is the first study to provide a functional phenotype of immune status by establishing a cytokine profile in milk SC from LPL, HPL, and BLV-negative cattle. Fil: Dolcini, Guillermina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina Fil: Ladera Gomez, Marla Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina Fil: Nieto Farías, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina Fil: Vater, Adrian Alejandro. Escuela de Educación Secundaria Agraria Nº1 Dr. Ramón Santamarina; Argentina Fil: Ceriani, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina I Congreso Argentino Microbiologia Veterinaria La Plata Argentina Asociacion Argentina Microbiologia |
description |
The bovine leukemia virus (BLV) is an exogenous retrovirus belonging to the Deltaretrovirus genus. This enveloped virus naturally infects B-lymphocytes causing enzootic bovine leukemia (EBL), which is spread worldwide, mainly in dairy cattle. The virus might produce cell-mediated immunity disorders, which may lead to opportunistic infections in BLV-infected cattle. Thus, the performance of dairy cattle is impaired, leading to large economic losses in the dairy industry. A study was carried out to investigate the levels of proviral load as well as mRNA expression of cytokines in peripheral blood mononuclear cells (PBMC) and milk somatic cells (SC) of LPL, HPL and uninfected cattle (controls). A total of 51 BLV-infected cows and 16 controls were included. The levels of proviral load in PBMC and SC as well as the expression levels of cytokine mRNA between LPL and HPL cattle were measured by real-time PCR (qPCR). LPL was detected in milk SC from BLV-infected animals. Three cytokines, IFN-γ, IL-12 and IL-6, were found to be up-regulated in the LPL group, and one cytokine, IL-10, was found to be down-regulated in PBMC. The expression levels of IL-12 mRNA in SC from LPL animals were higher than in the HPL group, while IFN-γ, IL-10 and IL-6 mRNA expression levels were significantly down-regulated. The results showed differences in cytokine expression in both PBMC and SC between LPL and HPL cattle. This is the first study to provide a functional phenotype of immune status by establishing a cytokine profile in milk SC from LPL, HPL, and BLV-negative cattle. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Congreso Book http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
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publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/170538 Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cell from BLV-infected catlle; I Congreso Argentino Microbiologia Veterinaria; La Plata; Argentina; 2021; 315-316 978-950-34-2018-8 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/170538 |
identifier_str_mv |
Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cell from BLV-infected catlle; I Congreso Argentino Microbiologia Veterinaria; La Plata; Argentina; 2021; 315-316 978-950-34-2018-8 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://congresos.unlp.edu.ar/microvet/wp-content/uploads/sites/19/2021/08/LIBRO-DE-RESUMENES-I-CMV-2021.pdf |
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info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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openAccess |
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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application/pdf application/pdf application/pdf |
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Nacional |
dc.publisher.none.fl_str_mv |
Universidad Nacional De La Plata. Facultad De Ciencias Veterinaria |
publisher.none.fl_str_mv |
Universidad Nacional De La Plata. Facultad De Ciencias Veterinaria |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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