Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cells from BLV-infected cattle

Autores
Dolcini, Guillermina Laura; Ladera Gomez, Marla Eliana; Nieto Farías, María Victoria; Váter, Adrián Alejandro; Ceriani, Maria Carolina
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The bovine leukemia virus (BLV) is an exogenous retrovirusbelonging to the Deltaretrovirus genus. This enveloped virus naturallyinfects B-lymphocytes causing enzootic bovine leukemia (EBL), whichis spread worldwide, mainly in dairy cattle. The virus might producecell-mediated immunity disorders, which may lead to opportunisticinfections in BLV-infected cattle. Thus, the performance of dairy cattleis impaired, leading to large economic losses in the dairy industry. Astudy was carried out to investigate the levels of proviral load as wellas mRNA expression of cytokines in peripheral blood mononuclearcells (PBMC) and milk somatic cells (SC) of low proviral load (LPL), highproviral load (HPL) and uninfected cattle (controls). A total of 51 BLVinfected cows and 16 controls were included. The levels of proviral load in PBMC and SC as well as the expression levels of cytokine mRNA between LPL and HPL cattle were measured by real-time PCR (qPCR). LPL was detected in milk SC from BLV-infected animals. Three cytokines, IFN-γ, IL-12 and IL-6, were found to be up-regulated in the LPL group, and one cytokine, IL-10, was found to be down-regulated in PBMC. The expression levels of IL-12 mRNA in SC from LPL animals were higher than in the HPL group, while IFN-γ, IL-10 and IL-6 mRNA expression levels were significantly down-regulated. The results showed differences in cytokine expression in both PBMC and SC between LPL and HPL cattle. This is the first study to provide a functional phenotype of immune status by establishing a cytokine profile in milk SC from LPL, HPL, and BLV-negative cattle.
Fil: Dolcini, Guillermina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Ladera Gomez, Marla Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Nieto Farías, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Váter, Adrián Alejandro. No especifíca;
Fil: Ceriani, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
I Congreso de Microbiología Veterinaria
Argentina
Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias
Materia
BLV
BOVINE CYTOKINES
PBMC
SOMATIC CELLS
MILK
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/225654
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cells from BLV-infected cattleDolcini, Guillermina LauraLadera Gomez, Marla ElianaNieto Farías, María VictoriaVáter, Adrián AlejandroCeriani, Maria CarolinaBLVBOVINE CYTOKINESPBMCSOMATIC CELLSMILKhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The bovine leukemia virus (BLV) is an exogenous retrovirusbelonging to the Deltaretrovirus genus. This enveloped virus naturallyinfects B-lymphocytes causing enzootic bovine leukemia (EBL), whichis spread worldwide, mainly in dairy cattle. The virus might producecell-mediated immunity disorders, which may lead to opportunisticinfections in BLV-infected cattle. Thus, the performance of dairy cattleis impaired, leading to large economic losses in the dairy industry. Astudy was carried out to investigate the levels of proviral load as wellas mRNA expression of cytokines in peripheral blood mononuclearcells (PBMC) and milk somatic cells (SC) of low proviral load (LPL), highproviral load (HPL) and uninfected cattle (controls). A total of 51 BLVinfected cows and 16 controls were included. The levels of proviral load in PBMC and SC as well as the expression levels of cytokine mRNA between LPL and HPL cattle were measured by real-time PCR (qPCR). LPL was detected in milk SC from BLV-infected animals. Three cytokines, IFN-γ, IL-12 and IL-6, were found to be up-regulated in the LPL group, and one cytokine, IL-10, was found to be down-regulated in PBMC. The expression levels of IL-12 mRNA in SC from LPL animals were higher than in the HPL group, while IFN-γ, IL-10 and IL-6 mRNA expression levels were significantly down-regulated. The results showed differences in cytokine expression in both PBMC and SC between LPL and HPL cattle. This is the first study to provide a functional phenotype of immune status by establishing a cytokine profile in milk SC from LPL, HPL, and BLV-negative cattle.Fil: Dolcini, Guillermina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Ladera Gomez, Marla Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Nieto Farías, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Váter, Adrián Alejandro. No especifíca;Fil: Ceriani, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaI Congreso de Microbiología VeterinariaArgentinaUniversidad Nacional de La Plata. Facultad de Ciencias VeterinariasUniversidad Nacional de La Plata. Facultad de Ciencias Veterinarias2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/225654Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cells from BLV-infected cattle; I Congreso de Microbiología Veterinaria; Argentina; 2021; 315-316978-950-34-2018-8CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://congresos.unlp.edu.ar/microvet/wp-content/uploads/sites/19/2021/08/LIBRO-DE-RESUMENES-I-CMV-2021.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:19:52Zoai:ri.conicet.gov.ar:11336/225654instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:19:52.407CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cells from BLV-infected cattle
title Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cells from BLV-infected cattle
spellingShingle Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cells from BLV-infected cattle
Dolcini, Guillermina Laura
BLV
BOVINE CYTOKINES
PBMC
SOMATIC CELLS
MILK
title_short Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cells from BLV-infected cattle
title_full Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cells from BLV-infected cattle
title_fullStr Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cells from BLV-infected cattle
title_full_unstemmed Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cells from BLV-infected cattle
title_sort Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cells from BLV-infected cattle
dc.creator.none.fl_str_mv Dolcini, Guillermina Laura
Ladera Gomez, Marla Eliana
Nieto Farías, María Victoria
Váter, Adrián Alejandro
Ceriani, Maria Carolina
author Dolcini, Guillermina Laura
author_facet Dolcini, Guillermina Laura
Ladera Gomez, Marla Eliana
Nieto Farías, María Victoria
Váter, Adrián Alejandro
Ceriani, Maria Carolina
author_role author
author2 Ladera Gomez, Marla Eliana
Nieto Farías, María Victoria
Váter, Adrián Alejandro
Ceriani, Maria Carolina
author2_role author
author
author
author
dc.subject.none.fl_str_mv BLV
BOVINE CYTOKINES
PBMC
SOMATIC CELLS
MILK
topic BLV
BOVINE CYTOKINES
PBMC
SOMATIC CELLS
MILK
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The bovine leukemia virus (BLV) is an exogenous retrovirusbelonging to the Deltaretrovirus genus. This enveloped virus naturallyinfects B-lymphocytes causing enzootic bovine leukemia (EBL), whichis spread worldwide, mainly in dairy cattle. The virus might producecell-mediated immunity disorders, which may lead to opportunisticinfections in BLV-infected cattle. Thus, the performance of dairy cattleis impaired, leading to large economic losses in the dairy industry. Astudy was carried out to investigate the levels of proviral load as wellas mRNA expression of cytokines in peripheral blood mononuclearcells (PBMC) and milk somatic cells (SC) of low proviral load (LPL), highproviral load (HPL) and uninfected cattle (controls). A total of 51 BLVinfected cows and 16 controls were included. The levels of proviral load in PBMC and SC as well as the expression levels of cytokine mRNA between LPL and HPL cattle were measured by real-time PCR (qPCR). LPL was detected in milk SC from BLV-infected animals. Three cytokines, IFN-γ, IL-12 and IL-6, were found to be up-regulated in the LPL group, and one cytokine, IL-10, was found to be down-regulated in PBMC. The expression levels of IL-12 mRNA in SC from LPL animals were higher than in the HPL group, while IFN-γ, IL-10 and IL-6 mRNA expression levels were significantly down-regulated. The results showed differences in cytokine expression in both PBMC and SC between LPL and HPL cattle. This is the first study to provide a functional phenotype of immune status by establishing a cytokine profile in milk SC from LPL, HPL, and BLV-negative cattle.
Fil: Dolcini, Guillermina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Ladera Gomez, Marla Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Nieto Farías, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Váter, Adrián Alejandro. No especifíca;
Fil: Ceriani, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
I Congreso de Microbiología Veterinaria
Argentina
Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias
description The bovine leukemia virus (BLV) is an exogenous retrovirusbelonging to the Deltaretrovirus genus. This enveloped virus naturallyinfects B-lymphocytes causing enzootic bovine leukemia (EBL), whichis spread worldwide, mainly in dairy cattle. The virus might producecell-mediated immunity disorders, which may lead to opportunisticinfections in BLV-infected cattle. Thus, the performance of dairy cattleis impaired, leading to large economic losses in the dairy industry. Astudy was carried out to investigate the levels of proviral load as wellas mRNA expression of cytokines in peripheral blood mononuclearcells (PBMC) and milk somatic cells (SC) of low proviral load (LPL), highproviral load (HPL) and uninfected cattle (controls). A total of 51 BLVinfected cows and 16 controls were included. The levels of proviral load in PBMC and SC as well as the expression levels of cytokine mRNA between LPL and HPL cattle were measured by real-time PCR (qPCR). LPL was detected in milk SC from BLV-infected animals. Three cytokines, IFN-γ, IL-12 and IL-6, were found to be up-regulated in the LPL group, and one cytokine, IL-10, was found to be down-regulated in PBMC. The expression levels of IL-12 mRNA in SC from LPL animals were higher than in the HPL group, while IFN-γ, IL-10 and IL-6 mRNA expression levels were significantly down-regulated. The results showed differences in cytokine expression in both PBMC and SC between LPL and HPL cattle. This is the first study to provide a functional phenotype of immune status by establishing a cytokine profile in milk SC from LPL, HPL, and BLV-negative cattle.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/225654
Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cells from BLV-infected cattle; I Congreso de Microbiología Veterinaria; Argentina; 2021; 315-316
978-950-34-2018-8
CONICET Digital
CONICET
url http://hdl.handle.net/11336/225654
identifier_str_mv Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cells from BLV-infected cattle; I Congreso de Microbiología Veterinaria; Argentina; 2021; 315-316
978-950-34-2018-8
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://congresos.unlp.edu.ar/microvet/wp-content/uploads/sites/19/2021/08/LIBRO-DE-RESUMENES-I-CMV-2021.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias
publisher.none.fl_str_mv Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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