Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cells from BLV-infected cattle

Autores
Dolcini, Guillermina Laura; Ladera Gómez, Marla Eliana; Nieto Farias, María Victoria; Váter, Adrián Alejandro; Ceriani, María Carolina
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The bovine leukemia virus (BLV) is an exogenous retrovirus belonging to the Deltaretrovirus genus. This enveloped virus naturally infects B-lymphocytes causing enzootic bovine leukemia (EBL), which is spread worldwide, mainly in dairy cattle. The virus might produce cell-mediated immunity disorders, which may lead to opportunistic infections in BLV-infected cattle. Thus, the performance of dairy cattle is impaired, leading to large economic losses in the dairy industry. A study was carried out to investigate the levels of proviral load as well as mRNA expression of cytokines in peripheral blood mononuclear cells (PBMC) and milk somatic cells (SC) of low proviral load (LPL), high proviral load (HPL) and uninfected cattle (controls). A total of 51 BLV- infected cows and 16 controls were included. The levels of proviral loadin PBMC and SC as well as the expression levels of cytokine mRNA between LPL and HPL cattle were measured by real-time PCR (qPCR).
Trabajo publicado en Cagliada, Maria del Pilar Lilia y Galosi, Cecilia Mónica (comps.). I Congreso de Microbiología Veterinaria. Libro de resúmenes. La Plata: Facultad de Ciencias Veterinarias, 2021.
Facultad de Ciencias Veterinarias
Materia
Ciencias Veterinarias
BLV
Bovine cytokines
PBMC
Somatic cells
Milk
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/122506
Acceder
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repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cells from BLV-infected cattleDolcini, Guillermina LauraLadera Gómez, Marla ElianaNieto Farias, María VictoriaVáter, Adrián AlejandroCeriani, María CarolinaCiencias VeterinariasBLVBovine cytokinesPBMCSomatic cellsMilkThe bovine leukemia virus (BLV) is an exogenous retrovirus belonging to the Deltaretrovirus genus. This enveloped virus naturally infects B-lymphocytes causing enzootic bovine leukemia (EBL), which is spread worldwide, mainly in dairy cattle. The virus might produce cell-mediated immunity disorders, which may lead to opportunistic infections in BLV-infected cattle. Thus, the performance of dairy cattle is impaired, leading to large economic losses in the dairy industry. A study was carried out to investigate the levels of proviral load as well as mRNA expression of cytokines in peripheral blood mononuclear cells (PBMC) and milk somatic cells (SC) of low proviral load (LPL), high proviral load (HPL) and uninfected cattle (controls). A total of 51 BLV- infected cows and 16 controls were included. The levels of proviral loadin PBMC and SC as well as the expression levels of cytokine mRNA between LPL and HPL cattle were measured by real-time PCR (qPCR).Trabajo publicado en Cagliada, Maria del Pilar Lilia y Galosi, Cecilia Mónica (comps.). <i>I Congreso de Microbiología Veterinaria. Libro de resúmenes</i>. La Plata: Facultad de Ciencias Veterinarias, 2021.Facultad de Ciencias Veterinarias2021info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/publishedVersionResumenhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdf315-316http://sedici.unlp.edu.ar/handle/10915/122506enginfo:eu-repo/semantics/altIdentifier/isbn/978-950-34-2018-8info:eu-repo/semantics/reference/hdl/10915/122095info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-17T10:11:54Zoai:sedici.unlp.edu.ar:10915/122506Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-17 10:11:55.134SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cells from BLV-infected cattle
title Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cells from BLV-infected cattle
spellingShingle Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cells from BLV-infected cattle
Dolcini, Guillermina Laura
Ciencias Veterinarias
BLV
Bovine cytokines
PBMC
Somatic cells
Milk
title_short Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cells from BLV-infected cattle
title_full Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cells from BLV-infected cattle
title_fullStr Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cells from BLV-infected cattle
title_full_unstemmed Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cells from BLV-infected cattle
title_sort Comparison of proviral load levels and mRNA expression of cytokines in peripheral blood mononuclear cells and milk somatic cells from BLV-infected cattle
dc.creator.none.fl_str_mv Dolcini, Guillermina Laura
Ladera Gómez, Marla Eliana
Nieto Farias, María Victoria
Váter, Adrián Alejandro
Ceriani, María Carolina
author Dolcini, Guillermina Laura
author_facet Dolcini, Guillermina Laura
Ladera Gómez, Marla Eliana
Nieto Farias, María Victoria
Váter, Adrián Alejandro
Ceriani, María Carolina
author_role author
author2 Ladera Gómez, Marla Eliana
Nieto Farias, María Victoria
Váter, Adrián Alejandro
Ceriani, María Carolina
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ciencias Veterinarias
BLV
Bovine cytokines
PBMC
Somatic cells
Milk
topic Ciencias Veterinarias
BLV
Bovine cytokines
PBMC
Somatic cells
Milk
dc.description.none.fl_txt_mv The bovine leukemia virus (BLV) is an exogenous retrovirus belonging to the Deltaretrovirus genus. This enveloped virus naturally infects B-lymphocytes causing enzootic bovine leukemia (EBL), which is spread worldwide, mainly in dairy cattle. The virus might produce cell-mediated immunity disorders, which may lead to opportunistic infections in BLV-infected cattle. Thus, the performance of dairy cattle is impaired, leading to large economic losses in the dairy industry. A study was carried out to investigate the levels of proviral load as well as mRNA expression of cytokines in peripheral blood mononuclear cells (PBMC) and milk somatic cells (SC) of low proviral load (LPL), high proviral load (HPL) and uninfected cattle (controls). A total of 51 BLV- infected cows and 16 controls were included. The levels of proviral loadin PBMC and SC as well as the expression levels of cytokine mRNA between LPL and HPL cattle were measured by real-time PCR (qPCR).
Trabajo publicado en Cagliada, Maria del Pilar Lilia y Galosi, Cecilia Mónica (comps.). <i>I Congreso de Microbiología Veterinaria. Libro de resúmenes</i>. La Plata: Facultad de Ciencias Veterinarias, 2021.
Facultad de Ciencias Veterinarias
description The bovine leukemia virus (BLV) is an exogenous retrovirus belonging to the Deltaretrovirus genus. This enveloped virus naturally infects B-lymphocytes causing enzootic bovine leukemia (EBL), which is spread worldwide, mainly in dairy cattle. The virus might produce cell-mediated immunity disorders, which may lead to opportunistic infections in BLV-infected cattle. Thus, the performance of dairy cattle is impaired, leading to large economic losses in the dairy industry. A study was carried out to investigate the levels of proviral load as well as mRNA expression of cytokines in peripheral blood mononuclear cells (PBMC) and milk somatic cells (SC) of low proviral load (LPL), high proviral load (HPL) and uninfected cattle (controls). A total of 51 BLV- infected cows and 16 controls were included. The levels of proviral loadin PBMC and SC as well as the expression levels of cytokine mRNA between LPL and HPL cattle were measured by real-time PCR (qPCR).
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/conferenceObject
info:eu-repo/semantics/publishedVersion
Resumen
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
format conferenceObject
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/122506
url http://sedici.unlp.edu.ar/handle/10915/122506
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/isbn/978-950-34-2018-8
info:eu-repo/semantics/reference/hdl/10915/122095
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
315-316
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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