Supramolecular Arrangement of Doxycycline with Sulfobutylether-β-Cyclodextrin: Impact on Nanostructuration with Chitosan, Drug Degradation and Antimicrobial Potency

Autores
Carvalho Feitosa, Renata; Souza Ribeiro Costa, Juliana; van Vliet Lima, Marcelo; Sawa Akioka Ishikawa, Elina; Cogo Müller, Karina; Bonin Okasaki, Fernando; Sabadini, Edvaldo; Garnero, Claudia; Longhi, Marcela Raquel; Lavayen, Vladimir; da Silva Júnior, Arnóbio Antônio; Oliveira Nascimento, Laura
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Doxycycline (DX) is a well-established and broad-spectrum antimicrobial drug. However, DX has drawbacks, such as physicochemical instability in aqueous media and bacterial resistance. The inclusion of drugs in cyclodextrin complexes and their loading into nanocarriers can overcome these limitations. Thus, we studied the DX/sulfobutylether-β-CD (SBE-β-CD) inclusion complex for the first time and used it to reticulate chitosan. The resulting particles were evaluated by their physicochemical characteristics and antibacterial activity. DX/SBE-β-CD complexes were characterized by nuclear magnetic resonance, infrared spectroscopy, thermal analysis, X-ray diffraction, and scanning electron microscopy (SEM), whereas DX-loaded nanoparticles were characterized by dynamic light scattering, SEM, and drug content. The partial inclusion of the DX molecule in CD happened in a 1:1 proportion and brought increased stability to solid DX upon thermal degradation. Chitosan-complex nanoparticles measured approximately 200 nm, with a narrow polydispersity and particles with sufficient drug encapsulation for microbiological studies. Both formulations preserved the antimicrobial activity of DX against Staphylococcus aureus, whereas DX/SBE-β-CD inclusion complexes were also active against Klebsiella pneumoniae, indicating the potential use of these formulations as drug delivery systems to treat local infections.
Fil: Carvalho Feitosa, Renata. Universidade Estadual de Campinas; Brasil
Fil: Souza Ribeiro Costa, Juliana. Universidade Estadual de Campinas; Brasil
Fil: van Vliet Lima, Marcelo. Universidade Estadual de Campinas; Brasil
Fil: Sawa Akioka Ishikawa, Elina. Universidade Estadual de Campinas; Brasil
Fil: Cogo Müller, Karina. Universidade Estadual de Campinas; Brasil
Fil: Bonin Okasaki, Fernando. Universidade Estadual de Campinas; Brasil
Fil: Sabadini, Edvaldo. Universidade Estadual de Campinas; Brasil
Fil: Garnero, Claudia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
Fil: Longhi, Marcela Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina
Fil: Lavayen, Vladimir. Universidade Federal do Rio Grande do Sul; Brasil
Fil: da Silva Júnior, Arnóbio Antônio. Universidade Federal do Rio Grande do Norte; Brasil
Fil: Oliveira Nascimento, Laura. Universidade Estadual de Campinas; Brasil
Materia
cyclodextrin
chitosan
doxycycline
nanoparticle
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/231546
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/231546
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Supramolecular Arrangement of Doxycycline with Sulfobutylether-β-Cyclodextrin: Impact on Nanostructuration with Chitosan, Drug Degradation and Antimicrobial PotencyCarvalho Feitosa, RenataSouza Ribeiro Costa, Julianavan Vliet Lima, MarceloSawa Akioka Ishikawa, ElinaCogo Müller, KarinaBonin Okasaki, FernandoSabadini, EdvaldoGarnero, ClaudiaLonghi, Marcela RaquelLavayen, Vladimirda Silva Júnior, Arnóbio AntônioOliveira Nascimento, Lauracyclodextrinchitosandoxycyclinenanoparticlehttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Doxycycline (DX) is a well-established and broad-spectrum antimicrobial drug. However, DX has drawbacks, such as physicochemical instability in aqueous media and bacterial resistance. The inclusion of drugs in cyclodextrin complexes and their loading into nanocarriers can overcome these limitations. Thus, we studied the DX/sulfobutylether-β-CD (SBE-β-CD) inclusion complex for the first time and used it to reticulate chitosan. The resulting particles were evaluated by their physicochemical characteristics and antibacterial activity. DX/SBE-β-CD complexes were characterized by nuclear magnetic resonance, infrared spectroscopy, thermal analysis, X-ray diffraction, and scanning electron microscopy (SEM), whereas DX-loaded nanoparticles were characterized by dynamic light scattering, SEM, and drug content. The partial inclusion of the DX molecule in CD happened in a 1:1 proportion and brought increased stability to solid DX upon thermal degradation. Chitosan-complex nanoparticles measured approximately 200 nm, with a narrow polydispersity and particles with sufficient drug encapsulation for microbiological studies. Both formulations preserved the antimicrobial activity of DX against Staphylococcus aureus, whereas DX/SBE-β-CD inclusion complexes were also active against Klebsiella pneumoniae, indicating the potential use of these formulations as drug delivery systems to treat local infections.Fil: Carvalho Feitosa, Renata. Universidade Estadual de Campinas; BrasilFil: Souza Ribeiro Costa, Juliana. Universidade Estadual de Campinas; BrasilFil: van Vliet Lima, Marcelo. Universidade Estadual de Campinas; BrasilFil: Sawa Akioka Ishikawa, Elina. Universidade Estadual de Campinas; BrasilFil: Cogo Müller, Karina. Universidade Estadual de Campinas; BrasilFil: Bonin Okasaki, Fernando. Universidade Estadual de Campinas; BrasilFil: Sabadini, Edvaldo. Universidade Estadual de Campinas; BrasilFil: Garnero, Claudia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; ArgentinaFil: Longhi, Marcela Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; ArgentinaFil: Lavayen, Vladimir. Universidade Federal do Rio Grande do Sul; BrasilFil: da Silva Júnior, Arnóbio Antônio. Universidade Federal do Rio Grande do Norte; BrasilFil: Oliveira Nascimento, Laura. Universidade Estadual de Campinas; BrasilMultidisciplinary Digital Publishing Institute2023-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/231546Carvalho Feitosa, Renata; Souza Ribeiro Costa, Juliana; van Vliet Lima, Marcelo; Sawa Akioka Ishikawa, Elina; Cogo Müller, Karina; et al.; Supramolecular Arrangement of Doxycycline with Sulfobutylether-β-Cyclodextrin: Impact on Nanostructuration with Chitosan, Drug Degradation and Antimicrobial Potency; Multidisciplinary Digital Publishing Institute; Pharmaceutics; 15; 4; 6-2023; 1-181999-4923CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/pharmaceutics15041285info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:20Zoai:ri.conicet.gov.ar:11336/231546instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:20.891CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Supramolecular Arrangement of Doxycycline with Sulfobutylether-β-Cyclodextrin: Impact on Nanostructuration with Chitosan, Drug Degradation and Antimicrobial Potency
title Supramolecular Arrangement of Doxycycline with Sulfobutylether-β-Cyclodextrin: Impact on Nanostructuration with Chitosan, Drug Degradation and Antimicrobial Potency
spellingShingle Supramolecular Arrangement of Doxycycline with Sulfobutylether-β-Cyclodextrin: Impact on Nanostructuration with Chitosan, Drug Degradation and Antimicrobial Potency
Carvalho Feitosa, Renata
cyclodextrin
chitosan
doxycycline
nanoparticle
title_short Supramolecular Arrangement of Doxycycline with Sulfobutylether-β-Cyclodextrin: Impact on Nanostructuration with Chitosan, Drug Degradation and Antimicrobial Potency
title_full Supramolecular Arrangement of Doxycycline with Sulfobutylether-β-Cyclodextrin: Impact on Nanostructuration with Chitosan, Drug Degradation and Antimicrobial Potency
title_fullStr Supramolecular Arrangement of Doxycycline with Sulfobutylether-β-Cyclodextrin: Impact on Nanostructuration with Chitosan, Drug Degradation and Antimicrobial Potency
title_full_unstemmed Supramolecular Arrangement of Doxycycline with Sulfobutylether-β-Cyclodextrin: Impact on Nanostructuration with Chitosan, Drug Degradation and Antimicrobial Potency
title_sort Supramolecular Arrangement of Doxycycline with Sulfobutylether-β-Cyclodextrin: Impact on Nanostructuration with Chitosan, Drug Degradation and Antimicrobial Potency
dc.creator.none.fl_str_mv Carvalho Feitosa, Renata
Souza Ribeiro Costa, Juliana
van Vliet Lima, Marcelo
Sawa Akioka Ishikawa, Elina
Cogo Müller, Karina
Bonin Okasaki, Fernando
Sabadini, Edvaldo
Garnero, Claudia
Longhi, Marcela Raquel
Lavayen, Vladimir
da Silva Júnior, Arnóbio Antônio
Oliveira Nascimento, Laura
author Carvalho Feitosa, Renata
author_facet Carvalho Feitosa, Renata
Souza Ribeiro Costa, Juliana
van Vliet Lima, Marcelo
Sawa Akioka Ishikawa, Elina
Cogo Müller, Karina
Bonin Okasaki, Fernando
Sabadini, Edvaldo
Garnero, Claudia
Longhi, Marcela Raquel
Lavayen, Vladimir
da Silva Júnior, Arnóbio Antônio
Oliveira Nascimento, Laura
author_role author
author2 Souza Ribeiro Costa, Juliana
van Vliet Lima, Marcelo
Sawa Akioka Ishikawa, Elina
Cogo Müller, Karina
Bonin Okasaki, Fernando
Sabadini, Edvaldo
Garnero, Claudia
Longhi, Marcela Raquel
Lavayen, Vladimir
da Silva Júnior, Arnóbio Antônio
Oliveira Nascimento, Laura
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv cyclodextrin
chitosan
doxycycline
nanoparticle
topic cyclodextrin
chitosan
doxycycline
nanoparticle
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Doxycycline (DX) is a well-established and broad-spectrum antimicrobial drug. However, DX has drawbacks, such as physicochemical instability in aqueous media and bacterial resistance. The inclusion of drugs in cyclodextrin complexes and their loading into nanocarriers can overcome these limitations. Thus, we studied the DX/sulfobutylether-β-CD (SBE-β-CD) inclusion complex for the first time and used it to reticulate chitosan. The resulting particles were evaluated by their physicochemical characteristics and antibacterial activity. DX/SBE-β-CD complexes were characterized by nuclear magnetic resonance, infrared spectroscopy, thermal analysis, X-ray diffraction, and scanning electron microscopy (SEM), whereas DX-loaded nanoparticles were characterized by dynamic light scattering, SEM, and drug content. The partial inclusion of the DX molecule in CD happened in a 1:1 proportion and brought increased stability to solid DX upon thermal degradation. Chitosan-complex nanoparticles measured approximately 200 nm, with a narrow polydispersity and particles with sufficient drug encapsulation for microbiological studies. Both formulations preserved the antimicrobial activity of DX against Staphylococcus aureus, whereas DX/SBE-β-CD inclusion complexes were also active against Klebsiella pneumoniae, indicating the potential use of these formulations as drug delivery systems to treat local infections.
Fil: Carvalho Feitosa, Renata. Universidade Estadual de Campinas; Brasil
Fil: Souza Ribeiro Costa, Juliana. Universidade Estadual de Campinas; Brasil
Fil: van Vliet Lima, Marcelo. Universidade Estadual de Campinas; Brasil
Fil: Sawa Akioka Ishikawa, Elina. Universidade Estadual de Campinas; Brasil
Fil: Cogo Müller, Karina. Universidade Estadual de Campinas; Brasil
Fil: Bonin Okasaki, Fernando. Universidade Estadual de Campinas; Brasil
Fil: Sabadini, Edvaldo. Universidade Estadual de Campinas; Brasil
Fil: Garnero, Claudia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
Fil: Longhi, Marcela Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina
Fil: Lavayen, Vladimir. Universidade Federal do Rio Grande do Sul; Brasil
Fil: da Silva Júnior, Arnóbio Antônio. Universidade Federal do Rio Grande do Norte; Brasil
Fil: Oliveira Nascimento, Laura. Universidade Estadual de Campinas; Brasil
description Doxycycline (DX) is a well-established and broad-spectrum antimicrobial drug. However, DX has drawbacks, such as physicochemical instability in aqueous media and bacterial resistance. The inclusion of drugs in cyclodextrin complexes and their loading into nanocarriers can overcome these limitations. Thus, we studied the DX/sulfobutylether-β-CD (SBE-β-CD) inclusion complex for the first time and used it to reticulate chitosan. The resulting particles were evaluated by their physicochemical characteristics and antibacterial activity. DX/SBE-β-CD complexes were characterized by nuclear magnetic resonance, infrared spectroscopy, thermal analysis, X-ray diffraction, and scanning electron microscopy (SEM), whereas DX-loaded nanoparticles were characterized by dynamic light scattering, SEM, and drug content. The partial inclusion of the DX molecule in CD happened in a 1:1 proportion and brought increased stability to solid DX upon thermal degradation. Chitosan-complex nanoparticles measured approximately 200 nm, with a narrow polydispersity and particles with sufficient drug encapsulation for microbiological studies. Both formulations preserved the antimicrobial activity of DX against Staphylococcus aureus, whereas DX/SBE-β-CD inclusion complexes were also active against Klebsiella pneumoniae, indicating the potential use of these formulations as drug delivery systems to treat local infections.
publishDate 2023
dc.date.none.fl_str_mv 2023-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/231546
Carvalho Feitosa, Renata; Souza Ribeiro Costa, Juliana; van Vliet Lima, Marcelo; Sawa Akioka Ishikawa, Elina; Cogo Müller, Karina; et al.; Supramolecular Arrangement of Doxycycline with Sulfobutylether-β-Cyclodextrin: Impact on Nanostructuration with Chitosan, Drug Degradation and Antimicrobial Potency; Multidisciplinary Digital Publishing Institute; Pharmaceutics; 15; 4; 6-2023; 1-18
1999-4923
CONICET Digital
CONICET
url http://hdl.handle.net/11336/231546
identifier_str_mv Carvalho Feitosa, Renata; Souza Ribeiro Costa, Juliana; van Vliet Lima, Marcelo; Sawa Akioka Ishikawa, Elina; Cogo Müller, Karina; et al.; Supramolecular Arrangement of Doxycycline with Sulfobutylether-β-Cyclodextrin: Impact on Nanostructuration with Chitosan, Drug Degradation and Antimicrobial Potency; Multidisciplinary Digital Publishing Institute; Pharmaceutics; 15; 4; 6-2023; 1-18
1999-4923
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/pharmaceutics15041285
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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