Histological and Histochemical Analysis of Austrocedrus chilensis Trees Healthy and Infected with Phytophthora austrocedri
- Autores
- Troncoso, Oscar Alberto; Greslebin, Alina Gabriela
- Año de publicación
- 2025
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The endemic Patagonian conifer, Austrocedrus chilensis, is threatened by the pathogenPhytophthora austrocedri. This study presents the first histological and histochemical analysisof A. chilensis affected by this pathogen. We examined the stem tissues of naturally infectedadult trees (over 30 years old) and artificially inoculated saplings (8–12 years old) to identifythe pathogen’s colonization strategies and the tree’s histological responses. Using lightand scanning electronic microscopy along with several histochemical techniques (Lugol,toluidine blue, vanillin-HCl, Phloroglucinol, Calcofluor white, and aniline blue), we foundthat P. austrocedri can grow in all active tissues, leading to cambium and parenchymanecrosis. The pathogen spreads through sieve cells and tracheids, moving to the adjacentcells via sieve plates and bordered pits and colonizing nearby parenchyma cells. Weobserved loss of starch in necrotic tissues. In contrast, starch accumulation and an increasein the number of polyphenolic cells occur in the healthy areas adjacent to the margins ofthe lesion, indicating a tree’s induced defense mechanisms. The tree’s responses includecambium reprogramming, which leads to the formation of traumatic resin ducts, alterationsin cell shape and size, and the deposition of phenolic compounds. We analyze the treeresponses and discuss their potential relationship with a methyl jasmonate-induced defenseand a hypersensitive-like response.
Fil: Troncoso, Oscar Alberto. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ingeniería - Sede Esquel; Argentina
Fil: Greslebin, Alina Gabriela. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Esquel; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Materia
-
austrocedrus root disease
forest diseases
mal del ciprés
scanning electron microscopy - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/265998
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Histological and Histochemical Analysis of Austrocedrus chilensis Trees Healthy and Infected with Phytophthora austrocedriTroncoso, Oscar AlbertoGreslebin, Alina Gabrielaaustrocedrus root diseaseforest diseasesmal del ciprésscanning electron microscopyhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The endemic Patagonian conifer, Austrocedrus chilensis, is threatened by the pathogenPhytophthora austrocedri. This study presents the first histological and histochemical analysisof A. chilensis affected by this pathogen. We examined the stem tissues of naturally infectedadult trees (over 30 years old) and artificially inoculated saplings (8–12 years old) to identifythe pathogen’s colonization strategies and the tree’s histological responses. Using lightand scanning electronic microscopy along with several histochemical techniques (Lugol,toluidine blue, vanillin-HCl, Phloroglucinol, Calcofluor white, and aniline blue), we foundthat P. austrocedri can grow in all active tissues, leading to cambium and parenchymanecrosis. The pathogen spreads through sieve cells and tracheids, moving to the adjacentcells via sieve plates and bordered pits and colonizing nearby parenchyma cells. Weobserved loss of starch in necrotic tissues. In contrast, starch accumulation and an increasein the number of polyphenolic cells occur in the healthy areas adjacent to the margins ofthe lesion, indicating a tree’s induced defense mechanisms. The tree’s responses includecambium reprogramming, which leads to the formation of traumatic resin ducts, alterationsin cell shape and size, and the deposition of phenolic compounds. We analyze the treeresponses and discuss their potential relationship with a methyl jasmonate-induced defenseand a hypersensitive-like response.Fil: Troncoso, Oscar Alberto. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ingeniería - Sede Esquel; ArgentinaFil: Greslebin, Alina Gabriela. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Esquel; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaMDPI2025-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/265998Troncoso, Oscar Alberto; Greslebin, Alina Gabriela; Histological and Histochemical Analysis of Austrocedrus chilensis Trees Healthy and Infected with Phytophthora austrocedri; MDPI; Forests; 16; 7; 6-2025; 1-191999-4907CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1999-4907/16/7/1073info:eu-repo/semantics/altIdentifier/doi/10.3390/f16071073info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:28:39Zoai:ri.conicet.gov.ar:11336/265998instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:28:39.677CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Histological and Histochemical Analysis of Austrocedrus chilensis Trees Healthy and Infected with Phytophthora austrocedri |
title |
Histological and Histochemical Analysis of Austrocedrus chilensis Trees Healthy and Infected with Phytophthora austrocedri |
spellingShingle |
Histological and Histochemical Analysis of Austrocedrus chilensis Trees Healthy and Infected with Phytophthora austrocedri Troncoso, Oscar Alberto austrocedrus root disease forest diseases mal del ciprés scanning electron microscopy |
title_short |
Histological and Histochemical Analysis of Austrocedrus chilensis Trees Healthy and Infected with Phytophthora austrocedri |
title_full |
Histological and Histochemical Analysis of Austrocedrus chilensis Trees Healthy and Infected with Phytophthora austrocedri |
title_fullStr |
Histological and Histochemical Analysis of Austrocedrus chilensis Trees Healthy and Infected with Phytophthora austrocedri |
title_full_unstemmed |
Histological and Histochemical Analysis of Austrocedrus chilensis Trees Healthy and Infected with Phytophthora austrocedri |
title_sort |
Histological and Histochemical Analysis of Austrocedrus chilensis Trees Healthy and Infected with Phytophthora austrocedri |
dc.creator.none.fl_str_mv |
Troncoso, Oscar Alberto Greslebin, Alina Gabriela |
author |
Troncoso, Oscar Alberto |
author_facet |
Troncoso, Oscar Alberto Greslebin, Alina Gabriela |
author_role |
author |
author2 |
Greslebin, Alina Gabriela |
author2_role |
author |
dc.subject.none.fl_str_mv |
austrocedrus root disease forest diseases mal del ciprés scanning electron microscopy |
topic |
austrocedrus root disease forest diseases mal del ciprés scanning electron microscopy |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The endemic Patagonian conifer, Austrocedrus chilensis, is threatened by the pathogenPhytophthora austrocedri. This study presents the first histological and histochemical analysisof A. chilensis affected by this pathogen. We examined the stem tissues of naturally infectedadult trees (over 30 years old) and artificially inoculated saplings (8–12 years old) to identifythe pathogen’s colonization strategies and the tree’s histological responses. Using lightand scanning electronic microscopy along with several histochemical techniques (Lugol,toluidine blue, vanillin-HCl, Phloroglucinol, Calcofluor white, and aniline blue), we foundthat P. austrocedri can grow in all active tissues, leading to cambium and parenchymanecrosis. The pathogen spreads through sieve cells and tracheids, moving to the adjacentcells via sieve plates and bordered pits and colonizing nearby parenchyma cells. Weobserved loss of starch in necrotic tissues. In contrast, starch accumulation and an increasein the number of polyphenolic cells occur in the healthy areas adjacent to the margins ofthe lesion, indicating a tree’s induced defense mechanisms. The tree’s responses includecambium reprogramming, which leads to the formation of traumatic resin ducts, alterationsin cell shape and size, and the deposition of phenolic compounds. We analyze the treeresponses and discuss their potential relationship with a methyl jasmonate-induced defenseand a hypersensitive-like response. Fil: Troncoso, Oscar Alberto. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ingeniería - Sede Esquel; Argentina Fil: Greslebin, Alina Gabriela. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Esquel; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
The endemic Patagonian conifer, Austrocedrus chilensis, is threatened by the pathogenPhytophthora austrocedri. This study presents the first histological and histochemical analysisof A. chilensis affected by this pathogen. We examined the stem tissues of naturally infectedadult trees (over 30 years old) and artificially inoculated saplings (8–12 years old) to identifythe pathogen’s colonization strategies and the tree’s histological responses. Using lightand scanning electronic microscopy along with several histochemical techniques (Lugol,toluidine blue, vanillin-HCl, Phloroglucinol, Calcofluor white, and aniline blue), we foundthat P. austrocedri can grow in all active tissues, leading to cambium and parenchymanecrosis. The pathogen spreads through sieve cells and tracheids, moving to the adjacentcells via sieve plates and bordered pits and colonizing nearby parenchyma cells. Weobserved loss of starch in necrotic tissues. In contrast, starch accumulation and an increasein the number of polyphenolic cells occur in the healthy areas adjacent to the margins ofthe lesion, indicating a tree’s induced defense mechanisms. The tree’s responses includecambium reprogramming, which leads to the formation of traumatic resin ducts, alterationsin cell shape and size, and the deposition of phenolic compounds. We analyze the treeresponses and discuss their potential relationship with a methyl jasmonate-induced defenseand a hypersensitive-like response. |
publishDate |
2025 |
dc.date.none.fl_str_mv |
2025-06 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/265998 Troncoso, Oscar Alberto; Greslebin, Alina Gabriela; Histological and Histochemical Analysis of Austrocedrus chilensis Trees Healthy and Infected with Phytophthora austrocedri; MDPI; Forests; 16; 7; 6-2025; 1-19 1999-4907 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/265998 |
identifier_str_mv |
Troncoso, Oscar Alberto; Greslebin, Alina Gabriela; Histological and Histochemical Analysis of Austrocedrus chilensis Trees Healthy and Infected with Phytophthora austrocedri; MDPI; Forests; 16; 7; 6-2025; 1-19 1999-4907 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1999-4907/16/7/1073 info:eu-repo/semantics/altIdentifier/doi/10.3390/f16071073 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614290817941504 |
score |
13.070432 |