Histological and Histochemical Analysis of Austrocedrus chilensis Trees Healthy and Infected with Phytophthora austrocedri

Autores
Troncoso, Oscar Alberto; Greslebin, Alina Gabriela
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The endemic Patagonian conifer, Austrocedrus chilensis, is threatened by the pathogenPhytophthora austrocedri. This study presents the first histological and histochemical analysisof A. chilensis affected by this pathogen. We examined the stem tissues of naturally infectedadult trees (over 30 years old) and artificially inoculated saplings (8–12 years old) to identifythe pathogen’s colonization strategies and the tree’s histological responses. Using lightand scanning electronic microscopy along with several histochemical techniques (Lugol,toluidine blue, vanillin-HCl, Phloroglucinol, Calcofluor white, and aniline blue), we foundthat P. austrocedri can grow in all active tissues, leading to cambium and parenchymanecrosis. The pathogen spreads through sieve cells and tracheids, moving to the adjacentcells via sieve plates and bordered pits and colonizing nearby parenchyma cells. Weobserved loss of starch in necrotic tissues. In contrast, starch accumulation and an increasein the number of polyphenolic cells occur in the healthy areas adjacent to the margins ofthe lesion, indicating a tree’s induced defense mechanisms. The tree’s responses includecambium reprogramming, which leads to the formation of traumatic resin ducts, alterationsin cell shape and size, and the deposition of phenolic compounds. We analyze the treeresponses and discuss their potential relationship with a methyl jasmonate-induced defenseand a hypersensitive-like response.
Fil: Troncoso, Oscar Alberto. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ingeniería - Sede Esquel; Argentina
Fil: Greslebin, Alina Gabriela. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Esquel; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
austrocedrus root disease
forest diseases
mal del ciprés
scanning electron microscopy
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/265998
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Histological and Histochemical Analysis of Austrocedrus chilensis Trees Healthy and Infected with Phytophthora austrocedriTroncoso, Oscar AlbertoGreslebin, Alina Gabrielaaustrocedrus root diseaseforest diseasesmal del ciprésscanning electron microscopyhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The endemic Patagonian conifer, Austrocedrus chilensis, is threatened by the pathogenPhytophthora austrocedri. This study presents the first histological and histochemical analysisof A. chilensis affected by this pathogen. We examined the stem tissues of naturally infectedadult trees (over 30 years old) and artificially inoculated saplings (8–12 years old) to identifythe pathogen’s colonization strategies and the tree’s histological responses. Using lightand scanning electronic microscopy along with several histochemical techniques (Lugol,toluidine blue, vanillin-HCl, Phloroglucinol, Calcofluor white, and aniline blue), we foundthat P. austrocedri can grow in all active tissues, leading to cambium and parenchymanecrosis. The pathogen spreads through sieve cells and tracheids, moving to the adjacentcells via sieve plates and bordered pits and colonizing nearby parenchyma cells. Weobserved loss of starch in necrotic tissues. In contrast, starch accumulation and an increasein the number of polyphenolic cells occur in the healthy areas adjacent to the margins ofthe lesion, indicating a tree’s induced defense mechanisms. The tree’s responses includecambium reprogramming, which leads to the formation of traumatic resin ducts, alterationsin cell shape and size, and the deposition of phenolic compounds. We analyze the treeresponses and discuss their potential relationship with a methyl jasmonate-induced defenseand a hypersensitive-like response.Fil: Troncoso, Oscar Alberto. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ingeniería - Sede Esquel; ArgentinaFil: Greslebin, Alina Gabriela. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Esquel; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaMDPI2025-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/265998Troncoso, Oscar Alberto; Greslebin, Alina Gabriela; Histological and Histochemical Analysis of Austrocedrus chilensis Trees Healthy and Infected with Phytophthora austrocedri; MDPI; Forests; 16; 7; 6-2025; 1-191999-4907CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1999-4907/16/7/1073info:eu-repo/semantics/altIdentifier/doi/10.3390/f16071073info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:01:00Zoai:ri.conicet.gov.ar:11336/265998instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:01:01.149CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Histological and Histochemical Analysis of Austrocedrus chilensis Trees Healthy and Infected with Phytophthora austrocedri
title Histological and Histochemical Analysis of Austrocedrus chilensis Trees Healthy and Infected with Phytophthora austrocedri
spellingShingle Histological and Histochemical Analysis of Austrocedrus chilensis Trees Healthy and Infected with Phytophthora austrocedri
Troncoso, Oscar Alberto
austrocedrus root disease
forest diseases
mal del ciprés
scanning electron microscopy
title_short Histological and Histochemical Analysis of Austrocedrus chilensis Trees Healthy and Infected with Phytophthora austrocedri
title_full Histological and Histochemical Analysis of Austrocedrus chilensis Trees Healthy and Infected with Phytophthora austrocedri
title_fullStr Histological and Histochemical Analysis of Austrocedrus chilensis Trees Healthy and Infected with Phytophthora austrocedri
title_full_unstemmed Histological and Histochemical Analysis of Austrocedrus chilensis Trees Healthy and Infected with Phytophthora austrocedri
title_sort Histological and Histochemical Analysis of Austrocedrus chilensis Trees Healthy and Infected with Phytophthora austrocedri
dc.creator.none.fl_str_mv Troncoso, Oscar Alberto
Greslebin, Alina Gabriela
author Troncoso, Oscar Alberto
author_facet Troncoso, Oscar Alberto
Greslebin, Alina Gabriela
author_role author
author2 Greslebin, Alina Gabriela
author2_role author
dc.subject.none.fl_str_mv austrocedrus root disease
forest diseases
mal del ciprés
scanning electron microscopy
topic austrocedrus root disease
forest diseases
mal del ciprés
scanning electron microscopy
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The endemic Patagonian conifer, Austrocedrus chilensis, is threatened by the pathogenPhytophthora austrocedri. This study presents the first histological and histochemical analysisof A. chilensis affected by this pathogen. We examined the stem tissues of naturally infectedadult trees (over 30 years old) and artificially inoculated saplings (8–12 years old) to identifythe pathogen’s colonization strategies and the tree’s histological responses. Using lightand scanning electronic microscopy along with several histochemical techniques (Lugol,toluidine blue, vanillin-HCl, Phloroglucinol, Calcofluor white, and aniline blue), we foundthat P. austrocedri can grow in all active tissues, leading to cambium and parenchymanecrosis. The pathogen spreads through sieve cells and tracheids, moving to the adjacentcells via sieve plates and bordered pits and colonizing nearby parenchyma cells. Weobserved loss of starch in necrotic tissues. In contrast, starch accumulation and an increasein the number of polyphenolic cells occur in the healthy areas adjacent to the margins ofthe lesion, indicating a tree’s induced defense mechanisms. The tree’s responses includecambium reprogramming, which leads to the formation of traumatic resin ducts, alterationsin cell shape and size, and the deposition of phenolic compounds. We analyze the treeresponses and discuss their potential relationship with a methyl jasmonate-induced defenseand a hypersensitive-like response.
Fil: Troncoso, Oscar Alberto. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ingeniería - Sede Esquel; Argentina
Fil: Greslebin, Alina Gabriela. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Esquel; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The endemic Patagonian conifer, Austrocedrus chilensis, is threatened by the pathogenPhytophthora austrocedri. This study presents the first histological and histochemical analysisof A. chilensis affected by this pathogen. We examined the stem tissues of naturally infectedadult trees (over 30 years old) and artificially inoculated saplings (8–12 years old) to identifythe pathogen’s colonization strategies and the tree’s histological responses. Using lightand scanning electronic microscopy along with several histochemical techniques (Lugol,toluidine blue, vanillin-HCl, Phloroglucinol, Calcofluor white, and aniline blue), we foundthat P. austrocedri can grow in all active tissues, leading to cambium and parenchymanecrosis. The pathogen spreads through sieve cells and tracheids, moving to the adjacentcells via sieve plates and bordered pits and colonizing nearby parenchyma cells. Weobserved loss of starch in necrotic tissues. In contrast, starch accumulation and an increasein the number of polyphenolic cells occur in the healthy areas adjacent to the margins ofthe lesion, indicating a tree’s induced defense mechanisms. The tree’s responses includecambium reprogramming, which leads to the formation of traumatic resin ducts, alterationsin cell shape and size, and the deposition of phenolic compounds. We analyze the treeresponses and discuss their potential relationship with a methyl jasmonate-induced defenseand a hypersensitive-like response.
publishDate 2025
dc.date.none.fl_str_mv 2025-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/265998
Troncoso, Oscar Alberto; Greslebin, Alina Gabriela; Histological and Histochemical Analysis of Austrocedrus chilensis Trees Healthy and Infected with Phytophthora austrocedri; MDPI; Forests; 16; 7; 6-2025; 1-19
1999-4907
CONICET Digital
CONICET
url http://hdl.handle.net/11336/265998
identifier_str_mv Troncoso, Oscar Alberto; Greslebin, Alina Gabriela; Histological and Histochemical Analysis of Austrocedrus chilensis Trees Healthy and Infected with Phytophthora austrocedri; MDPI; Forests; 16; 7; 6-2025; 1-19
1999-4907
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1999-4907/16/7/1073
info:eu-repo/semantics/altIdentifier/doi/10.3390/f16071073
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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