Phytophthora austrocedri elicitates changes in diterpene profile of austrocedrus chilensis

Autores
Olate, Verónica Rachel; Vélez, María Laura; Greslebin, Alina Gabriela; Schmeda Hirschmann, Guillermo
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The populations of the Andean Cupressaceae Austrocedrus chilensis have been severely affected by a disease caused by the phytopathogenic fungus Phytophthora austrocedri. A study was undertaken to disclose changes in the resin composition of P. austrocedri-infected individuals, including naturally infected and artificially inoculated trees, compared with healthy A. chilensis trees. GC-MS and 1H-NMR studies showed a clear differentiation among healthy and infected resins, with the diterpene isopimara-8(9),15-dien-19-ol as a relevant constituent in resins from infected trees. The effect of resin fractions from P. austrocedri infected trees on the pathogen was assessed by measuring the mycelial growth in agar plates. The most active fractions from resin obtained from infected trees inhibited fungal growth by nearly 50% at 1 mg/dish (35.37 μg/cm2). The main constituent in the active fractions were 18-hydroxymanool and the aldehyde torulosal. Both compounds are oxidation products of manool and can be a chemical response of the tree to the pathogen or be formed from the pathogen as a biotransformation product of manool by microbial oxidation. While the diterpene profiles from A. chilensis tree resins can easily differentiate healthy and P. austrocedri infected individuals, the possible conversion of manool to the antifungal derivatives 4 and 6 by the microorganism remains to be established.
Fil: Olate, Verónica Rachel. Universidad de Talca; Chile
Fil: Vélez, María Laura. Centro de Investigación y Extensión Forestal Andino Patagónico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Greslebin, Alina Gabriela. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Esquel; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Schmeda Hirschmann, Guillermo. Universidad de Talca; Chile
Materia
PHYTOPHTHORA AUSTROCEDRI
AUSTROCEDRUS CHILENSIS
RESIN DITERPENES
CUPRESSACEAE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/98560
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Phytophthora austrocedri elicitates changes in diterpene profile of austrocedrus chilensisOlate, Verónica RachelVélez, María LauraGreslebin, Alina GabrielaSchmeda Hirschmann, GuillermoPHYTOPHTHORA AUSTROCEDRIAUSTROCEDRUS CHILENSISRESIN DITERPENESCUPRESSACEAEhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The populations of the Andean Cupressaceae Austrocedrus chilensis have been severely affected by a disease caused by the phytopathogenic fungus Phytophthora austrocedri. A study was undertaken to disclose changes in the resin composition of P. austrocedri-infected individuals, including naturally infected and artificially inoculated trees, compared with healthy A. chilensis trees. GC-MS and 1H-NMR studies showed a clear differentiation among healthy and infected resins, with the diterpene isopimara-8(9),15-dien-19-ol as a relevant constituent in resins from infected trees. The effect of resin fractions from P. austrocedri infected trees on the pathogen was assessed by measuring the mycelial growth in agar plates. The most active fractions from resin obtained from infected trees inhibited fungal growth by nearly 50% at 1 mg/dish (35.37 μg/cm2). The main constituent in the active fractions were 18-hydroxymanool and the aldehyde torulosal. Both compounds are oxidation products of manool and can be a chemical response of the tree to the pathogen or be formed from the pathogen as a biotransformation product of manool by microbial oxidation. While the diterpene profiles from A. chilensis tree resins can easily differentiate healthy and P. austrocedri infected individuals, the possible conversion of manool to the antifungal derivatives 4 and 6 by the microorganism remains to be established.Fil: Olate, Verónica Rachel. Universidad de Talca; ChileFil: Vélez, María Laura. Centro de Investigación y Extensión Forestal Andino Patagónico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Greslebin, Alina Gabriela. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Esquel; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Schmeda Hirschmann, Guillermo. Universidad de Talca; ChileMolecular Diversity Preservation International2015-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/98560Olate, Verónica Rachel; Vélez, María Laura; Greslebin, Alina Gabriela; Schmeda Hirschmann, Guillermo; Phytophthora austrocedri elicitates changes in diterpene profile of austrocedrus chilensis; Molecular Diversity Preservation International; Molecules; 20; 8; 8-2015; 15084-150971420-3049CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1420-3049/20/8/15084info:eu-repo/semantics/altIdentifier/doi/10.3390/molecules200815084info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:58:52Zoai:ri.conicet.gov.ar:11336/98560instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:58:52.877CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Phytophthora austrocedri elicitates changes in diterpene profile of austrocedrus chilensis
title Phytophthora austrocedri elicitates changes in diterpene profile of austrocedrus chilensis
spellingShingle Phytophthora austrocedri elicitates changes in diterpene profile of austrocedrus chilensis
Olate, Verónica Rachel
PHYTOPHTHORA AUSTROCEDRI
AUSTROCEDRUS CHILENSIS
RESIN DITERPENES
CUPRESSACEAE
title_short Phytophthora austrocedri elicitates changes in diterpene profile of austrocedrus chilensis
title_full Phytophthora austrocedri elicitates changes in diterpene profile of austrocedrus chilensis
title_fullStr Phytophthora austrocedri elicitates changes in diterpene profile of austrocedrus chilensis
title_full_unstemmed Phytophthora austrocedri elicitates changes in diterpene profile of austrocedrus chilensis
title_sort Phytophthora austrocedri elicitates changes in diterpene profile of austrocedrus chilensis
dc.creator.none.fl_str_mv Olate, Verónica Rachel
Vélez, María Laura
Greslebin, Alina Gabriela
Schmeda Hirschmann, Guillermo
author Olate, Verónica Rachel
author_facet Olate, Verónica Rachel
Vélez, María Laura
Greslebin, Alina Gabriela
Schmeda Hirschmann, Guillermo
author_role author
author2 Vélez, María Laura
Greslebin, Alina Gabriela
Schmeda Hirschmann, Guillermo
author2_role author
author
author
dc.subject.none.fl_str_mv PHYTOPHTHORA AUSTROCEDRI
AUSTROCEDRUS CHILENSIS
RESIN DITERPENES
CUPRESSACEAE
topic PHYTOPHTHORA AUSTROCEDRI
AUSTROCEDRUS CHILENSIS
RESIN DITERPENES
CUPRESSACEAE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The populations of the Andean Cupressaceae Austrocedrus chilensis have been severely affected by a disease caused by the phytopathogenic fungus Phytophthora austrocedri. A study was undertaken to disclose changes in the resin composition of P. austrocedri-infected individuals, including naturally infected and artificially inoculated trees, compared with healthy A. chilensis trees. GC-MS and 1H-NMR studies showed a clear differentiation among healthy and infected resins, with the diterpene isopimara-8(9),15-dien-19-ol as a relevant constituent in resins from infected trees. The effect of resin fractions from P. austrocedri infected trees on the pathogen was assessed by measuring the mycelial growth in agar plates. The most active fractions from resin obtained from infected trees inhibited fungal growth by nearly 50% at 1 mg/dish (35.37 μg/cm2). The main constituent in the active fractions were 18-hydroxymanool and the aldehyde torulosal. Both compounds are oxidation products of manool and can be a chemical response of the tree to the pathogen or be formed from the pathogen as a biotransformation product of manool by microbial oxidation. While the diterpene profiles from A. chilensis tree resins can easily differentiate healthy and P. austrocedri infected individuals, the possible conversion of manool to the antifungal derivatives 4 and 6 by the microorganism remains to be established.
Fil: Olate, Verónica Rachel. Universidad de Talca; Chile
Fil: Vélez, María Laura. Centro de Investigación y Extensión Forestal Andino Patagónico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Greslebin, Alina Gabriela. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Esquel; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Schmeda Hirschmann, Guillermo. Universidad de Talca; Chile
description The populations of the Andean Cupressaceae Austrocedrus chilensis have been severely affected by a disease caused by the phytopathogenic fungus Phytophthora austrocedri. A study was undertaken to disclose changes in the resin composition of P. austrocedri-infected individuals, including naturally infected and artificially inoculated trees, compared with healthy A. chilensis trees. GC-MS and 1H-NMR studies showed a clear differentiation among healthy and infected resins, with the diterpene isopimara-8(9),15-dien-19-ol as a relevant constituent in resins from infected trees. The effect of resin fractions from P. austrocedri infected trees on the pathogen was assessed by measuring the mycelial growth in agar plates. The most active fractions from resin obtained from infected trees inhibited fungal growth by nearly 50% at 1 mg/dish (35.37 μg/cm2). The main constituent in the active fractions were 18-hydroxymanool and the aldehyde torulosal. Both compounds are oxidation products of manool and can be a chemical response of the tree to the pathogen or be formed from the pathogen as a biotransformation product of manool by microbial oxidation. While the diterpene profiles from A. chilensis tree resins can easily differentiate healthy and P. austrocedri infected individuals, the possible conversion of manool to the antifungal derivatives 4 and 6 by the microorganism remains to be established.
publishDate 2015
dc.date.none.fl_str_mv 2015-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/98560
Olate, Verónica Rachel; Vélez, María Laura; Greslebin, Alina Gabriela; Schmeda Hirschmann, Guillermo; Phytophthora austrocedri elicitates changes in diterpene profile of austrocedrus chilensis; Molecular Diversity Preservation International; Molecules; 20; 8; 8-2015; 15084-15097
1420-3049
CONICET Digital
CONICET
url http://hdl.handle.net/11336/98560
identifier_str_mv Olate, Verónica Rachel; Vélez, María Laura; Greslebin, Alina Gabriela; Schmeda Hirschmann, Guillermo; Phytophthora austrocedri elicitates changes in diterpene profile of austrocedrus chilensis; Molecular Diversity Preservation International; Molecules; 20; 8; 8-2015; 15084-15097
1420-3049
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1420-3049/20/8/15084
info:eu-repo/semantics/altIdentifier/doi/10.3390/molecules200815084
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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