Phytophthora austrocedri elicitates changes in diterpene profile of austrocedrus chilensis
- Autores
- Olate, Verónica Rachel; Vélez, María Laura; Greslebin, Alina Gabriela; Schmeda Hirschmann, Guillermo
- Año de publicación
- 2015
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The populations of the Andean Cupressaceae Austrocedrus chilensis have been severely affected by a disease caused by the phytopathogenic fungus Phytophthora austrocedri. A study was undertaken to disclose changes in the resin composition of P. austrocedri-infected individuals, including naturally infected and artificially inoculated trees, compared with healthy A. chilensis trees. GC-MS and 1H-NMR studies showed a clear differentiation among healthy and infected resins, with the diterpene isopimara-8(9),15-dien-19-ol as a relevant constituent in resins from infected trees. The effect of resin fractions from P. austrocedri infected trees on the pathogen was assessed by measuring the mycelial growth in agar plates. The most active fractions from resin obtained from infected trees inhibited fungal growth by nearly 50% at 1 mg/dish (35.37 μg/cm2). The main constituent in the active fractions were 18-hydroxymanool and the aldehyde torulosal. Both compounds are oxidation products of manool and can be a chemical response of the tree to the pathogen or be formed from the pathogen as a biotransformation product of manool by microbial oxidation. While the diterpene profiles from A. chilensis tree resins can easily differentiate healthy and P. austrocedri infected individuals, the possible conversion of manool to the antifungal derivatives 4 and 6 by the microorganism remains to be established.
Fil: Olate, Verónica Rachel. Universidad de Talca; Chile
Fil: Vélez, María Laura. Centro de Investigación y Extensión Forestal Andino Patagónico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Greslebin, Alina Gabriela. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Esquel; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Schmeda Hirschmann, Guillermo. Universidad de Talca; Chile - Materia
-
PHYTOPHTHORA AUSTROCEDRI
AUSTROCEDRUS CHILENSIS
RESIN DITERPENES
CUPRESSACEAE - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/98560
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Phytophthora austrocedri elicitates changes in diterpene profile of austrocedrus chilensisOlate, Verónica RachelVélez, María LauraGreslebin, Alina GabrielaSchmeda Hirschmann, GuillermoPHYTOPHTHORA AUSTROCEDRIAUSTROCEDRUS CHILENSISRESIN DITERPENESCUPRESSACEAEhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The populations of the Andean Cupressaceae Austrocedrus chilensis have been severely affected by a disease caused by the phytopathogenic fungus Phytophthora austrocedri. A study was undertaken to disclose changes in the resin composition of P. austrocedri-infected individuals, including naturally infected and artificially inoculated trees, compared with healthy A. chilensis trees. GC-MS and 1H-NMR studies showed a clear differentiation among healthy and infected resins, with the diterpene isopimara-8(9),15-dien-19-ol as a relevant constituent in resins from infected trees. The effect of resin fractions from P. austrocedri infected trees on the pathogen was assessed by measuring the mycelial growth in agar plates. The most active fractions from resin obtained from infected trees inhibited fungal growth by nearly 50% at 1 mg/dish (35.37 μg/cm2). The main constituent in the active fractions were 18-hydroxymanool and the aldehyde torulosal. Both compounds are oxidation products of manool and can be a chemical response of the tree to the pathogen or be formed from the pathogen as a biotransformation product of manool by microbial oxidation. While the diterpene profiles from A. chilensis tree resins can easily differentiate healthy and P. austrocedri infected individuals, the possible conversion of manool to the antifungal derivatives 4 and 6 by the microorganism remains to be established.Fil: Olate, Verónica Rachel. Universidad de Talca; ChileFil: Vélez, María Laura. Centro de Investigación y Extensión Forestal Andino Patagónico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Greslebin, Alina Gabriela. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Esquel; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Schmeda Hirschmann, Guillermo. Universidad de Talca; ChileMolecular Diversity Preservation International2015-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/98560Olate, Verónica Rachel; Vélez, María Laura; Greslebin, Alina Gabriela; Schmeda Hirschmann, Guillermo; Phytophthora austrocedri elicitates changes in diterpene profile of austrocedrus chilensis; Molecular Diversity Preservation International; Molecules; 20; 8; 8-2015; 15084-150971420-3049CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1420-3049/20/8/15084info:eu-repo/semantics/altIdentifier/doi/10.3390/molecules200815084info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:02:45Zoai:ri.conicet.gov.ar:11336/98560instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:02:45.389CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Phytophthora austrocedri elicitates changes in diterpene profile of austrocedrus chilensis |
title |
Phytophthora austrocedri elicitates changes in diterpene profile of austrocedrus chilensis |
spellingShingle |
Phytophthora austrocedri elicitates changes in diterpene profile of austrocedrus chilensis Olate, Verónica Rachel PHYTOPHTHORA AUSTROCEDRI AUSTROCEDRUS CHILENSIS RESIN DITERPENES CUPRESSACEAE |
title_short |
Phytophthora austrocedri elicitates changes in diterpene profile of austrocedrus chilensis |
title_full |
Phytophthora austrocedri elicitates changes in diterpene profile of austrocedrus chilensis |
title_fullStr |
Phytophthora austrocedri elicitates changes in diterpene profile of austrocedrus chilensis |
title_full_unstemmed |
Phytophthora austrocedri elicitates changes in diterpene profile of austrocedrus chilensis |
title_sort |
Phytophthora austrocedri elicitates changes in diterpene profile of austrocedrus chilensis |
dc.creator.none.fl_str_mv |
Olate, Verónica Rachel Vélez, María Laura Greslebin, Alina Gabriela Schmeda Hirschmann, Guillermo |
author |
Olate, Verónica Rachel |
author_facet |
Olate, Verónica Rachel Vélez, María Laura Greslebin, Alina Gabriela Schmeda Hirschmann, Guillermo |
author_role |
author |
author2 |
Vélez, María Laura Greslebin, Alina Gabriela Schmeda Hirschmann, Guillermo |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
PHYTOPHTHORA AUSTROCEDRI AUSTROCEDRUS CHILENSIS RESIN DITERPENES CUPRESSACEAE |
topic |
PHYTOPHTHORA AUSTROCEDRI AUSTROCEDRUS CHILENSIS RESIN DITERPENES CUPRESSACEAE |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The populations of the Andean Cupressaceae Austrocedrus chilensis have been severely affected by a disease caused by the phytopathogenic fungus Phytophthora austrocedri. A study was undertaken to disclose changes in the resin composition of P. austrocedri-infected individuals, including naturally infected and artificially inoculated trees, compared with healthy A. chilensis trees. GC-MS and 1H-NMR studies showed a clear differentiation among healthy and infected resins, with the diterpene isopimara-8(9),15-dien-19-ol as a relevant constituent in resins from infected trees. The effect of resin fractions from P. austrocedri infected trees on the pathogen was assessed by measuring the mycelial growth in agar plates. The most active fractions from resin obtained from infected trees inhibited fungal growth by nearly 50% at 1 mg/dish (35.37 μg/cm2). The main constituent in the active fractions were 18-hydroxymanool and the aldehyde torulosal. Both compounds are oxidation products of manool and can be a chemical response of the tree to the pathogen or be formed from the pathogen as a biotransformation product of manool by microbial oxidation. While the diterpene profiles from A. chilensis tree resins can easily differentiate healthy and P. austrocedri infected individuals, the possible conversion of manool to the antifungal derivatives 4 and 6 by the microorganism remains to be established. Fil: Olate, Verónica Rachel. Universidad de Talca; Chile Fil: Vélez, María Laura. Centro de Investigación y Extensión Forestal Andino Patagónico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Greslebin, Alina Gabriela. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Esquel; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Schmeda Hirschmann, Guillermo. Universidad de Talca; Chile |
description |
The populations of the Andean Cupressaceae Austrocedrus chilensis have been severely affected by a disease caused by the phytopathogenic fungus Phytophthora austrocedri. A study was undertaken to disclose changes in the resin composition of P. austrocedri-infected individuals, including naturally infected and artificially inoculated trees, compared with healthy A. chilensis trees. GC-MS and 1H-NMR studies showed a clear differentiation among healthy and infected resins, with the diterpene isopimara-8(9),15-dien-19-ol as a relevant constituent in resins from infected trees. The effect of resin fractions from P. austrocedri infected trees on the pathogen was assessed by measuring the mycelial growth in agar plates. The most active fractions from resin obtained from infected trees inhibited fungal growth by nearly 50% at 1 mg/dish (35.37 μg/cm2). The main constituent in the active fractions were 18-hydroxymanool and the aldehyde torulosal. Both compounds are oxidation products of manool and can be a chemical response of the tree to the pathogen or be formed from the pathogen as a biotransformation product of manool by microbial oxidation. While the diterpene profiles from A. chilensis tree resins can easily differentiate healthy and P. austrocedri infected individuals, the possible conversion of manool to the antifungal derivatives 4 and 6 by the microorganism remains to be established. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-08 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/98560 Olate, Verónica Rachel; Vélez, María Laura; Greslebin, Alina Gabriela; Schmeda Hirschmann, Guillermo; Phytophthora austrocedri elicitates changes in diterpene profile of austrocedrus chilensis; Molecular Diversity Preservation International; Molecules; 20; 8; 8-2015; 15084-15097 1420-3049 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/98560 |
identifier_str_mv |
Olate, Verónica Rachel; Vélez, María Laura; Greslebin, Alina Gabriela; Schmeda Hirschmann, Guillermo; Phytophthora austrocedri elicitates changes in diterpene profile of austrocedrus chilensis; Molecular Diversity Preservation International; Molecules; 20; 8; 8-2015; 15084-15097 1420-3049 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1420-3049/20/8/15084 info:eu-repo/semantics/altIdentifier/doi/10.3390/molecules200815084 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Molecular Diversity Preservation International |
publisher.none.fl_str_mv |
Molecular Diversity Preservation International |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613835276681216 |
score |
13.070432 |