Evidence of low levels of genetic diversity for the Phytophthora austrocedrae population in Patagonia, Argentina

Autores
Velez, Maria Laura; Coetzee, M. P. A.; Wingfield, M. J.; Rajchenberg, Mario; Greslebin, Alina Gabriela
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Phytophthora austrocedrae is a recently discovered pathogen that causes severe mortality of Austrocedrus chilensis in Patagonia. The high level of susceptibility of the host tree, together with the distribution pattern of the pathogen, have led to the hypothesis that P. austrocedrae was introduced into Argentina. The aim of this study was to assess the population structure of P. austrocedrae isolates from Argentina in order to gain an understanding of the origin and spread of the pathogen. Genetic diversity was determined based on amplified fragment length polymorphisms (AFLPs). In total, 48 isolates of P. austrocedrae were obtained from infected A. chilensis trees, representing the geographical range of the host. Four primer combinations were used for the AFLP analysis. Of the 332 scored bands, 12% were polymorphic. Gene diversity (h) ranged from 0·01 to 0·03; the Shannon index (I) ranged from 0·01 to 0·04. A high degree of genetic similarity was observed among the isolates (pairwise S values = 0·958–1; 0·993 ± 0·009, mean ± SD). A frequency histogram showed that most of the isolate pairs were identical. Principal coordinate analysis using three-dimensional plots did not group any of the isolates based on their geographical origin. The low genetic diversity (within and between sites) and absence of population structure linked to geographic origin, together with the aggressiveness of the pathogen and the disease progression pattern, suggest that P. austrocedrae might have been introduced into Argentina.
Fil: Velez, Maria Laura. Centro de Investigación y Extensión Forestal Andino Patagónico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Coetzee, M. P. A.. University of Pretoria; Sudáfrica
Fil: Wingfield, M. J.. University of Pretoria; Sudáfrica
Fil: Rajchenberg, Mario. Centro de Investigación y Extensión Forestal Andino Patagónico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Greslebin, Alina Gabriela. Centro de Investigación y Extensión Forestal Andino Patagónico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Aflp Analysis
Austrocedrus Chilensis
Austrocedrus Root Disease
Biological Invasions
Forest Phytophthoras
Mal del Cipres
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/17351
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/17351
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evidence of low levels of genetic diversity for the Phytophthora austrocedrae population in Patagonia, ArgentinaVelez, Maria LauraCoetzee, M. P. A.Wingfield, M. J.Rajchenberg, MarioGreslebin, Alina GabrielaAflp AnalysisAustrocedrus ChilensisAustrocedrus Root DiseaseBiological InvasionsForest PhytophthorasMal del Cipreshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Phytophthora austrocedrae is a recently discovered pathogen that causes severe mortality of Austrocedrus chilensis in Patagonia. The high level of susceptibility of the host tree, together with the distribution pattern of the pathogen, have led to the hypothesis that P. austrocedrae was introduced into Argentina. The aim of this study was to assess the population structure of P. austrocedrae isolates from Argentina in order to gain an understanding of the origin and spread of the pathogen. Genetic diversity was determined based on amplified fragment length polymorphisms (AFLPs). In total, 48 isolates of P. austrocedrae were obtained from infected A. chilensis trees, representing the geographical range of the host. Four primer combinations were used for the AFLP analysis. Of the 332 scored bands, 12% were polymorphic. Gene diversity (h) ranged from 0·01 to 0·03; the Shannon index (I) ranged from 0·01 to 0·04. A high degree of genetic similarity was observed among the isolates (pairwise S values = 0·958–1; 0·993 ± 0·009, mean ± SD). A frequency histogram showed that most of the isolate pairs were identical. Principal coordinate analysis using three-dimensional plots did not group any of the isolates based on their geographical origin. The low genetic diversity (within and between sites) and absence of population structure linked to geographic origin, together with the aggressiveness of the pathogen and the disease progression pattern, suggest that P. austrocedrae might have been introduced into Argentina.Fil: Velez, Maria Laura. Centro de Investigación y Extensión Forestal Andino Patagónico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Coetzee, M. P. A.. University of Pretoria; SudáfricaFil: Wingfield, M. J.. University of Pretoria; SudáfricaFil: Rajchenberg, Mario. Centro de Investigación y Extensión Forestal Andino Patagónico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Greslebin, Alina Gabriela. Centro de Investigación y Extensión Forestal Andino Patagónico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWiley2014-02-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/17351Velez, Maria Laura; Coetzee, M. P. A.; Wingfield, M. J.; Rajchenberg, Mario; Greslebin, Alina Gabriela; Evidence of low levels of genetic diversity for the Phytophthora austrocedrae population in Patagonia, Argentina; Wiley; Plant Pathology; 63; 1; 27-2-2014; 212-2200032-0862enginfo:eu-repo/semantics/altIdentifier/doi/10.1111/ppa.12067info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/ppa.12067/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:36:33Zoai:ri.conicet.gov.ar:11336/17351instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:36:33.446CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evidence of low levels of genetic diversity for the Phytophthora austrocedrae population in Patagonia, Argentina
title Evidence of low levels of genetic diversity for the Phytophthora austrocedrae population in Patagonia, Argentina
spellingShingle Evidence of low levels of genetic diversity for the Phytophthora austrocedrae population in Patagonia, Argentina
Velez, Maria Laura
Aflp Analysis
Austrocedrus Chilensis
Austrocedrus Root Disease
Biological Invasions
Forest Phytophthoras
Mal del Cipres
title_short Evidence of low levels of genetic diversity for the Phytophthora austrocedrae population in Patagonia, Argentina
title_full Evidence of low levels of genetic diversity for the Phytophthora austrocedrae population in Patagonia, Argentina
title_fullStr Evidence of low levels of genetic diversity for the Phytophthora austrocedrae population in Patagonia, Argentina
title_full_unstemmed Evidence of low levels of genetic diversity for the Phytophthora austrocedrae population in Patagonia, Argentina
title_sort Evidence of low levels of genetic diversity for the Phytophthora austrocedrae population in Patagonia, Argentina
dc.creator.none.fl_str_mv Velez, Maria Laura
Coetzee, M. P. A.
Wingfield, M. J.
Rajchenberg, Mario
Greslebin, Alina Gabriela
author Velez, Maria Laura
author_facet Velez, Maria Laura
Coetzee, M. P. A.
Wingfield, M. J.
Rajchenberg, Mario
Greslebin, Alina Gabriela
author_role author
author2 Coetzee, M. P. A.
Wingfield, M. J.
Rajchenberg, Mario
Greslebin, Alina Gabriela
author2_role author
author
author
author
dc.subject.none.fl_str_mv Aflp Analysis
Austrocedrus Chilensis
Austrocedrus Root Disease
Biological Invasions
Forest Phytophthoras
Mal del Cipres
topic Aflp Analysis
Austrocedrus Chilensis
Austrocedrus Root Disease
Biological Invasions
Forest Phytophthoras
Mal del Cipres
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Phytophthora austrocedrae is a recently discovered pathogen that causes severe mortality of Austrocedrus chilensis in Patagonia. The high level of susceptibility of the host tree, together with the distribution pattern of the pathogen, have led to the hypothesis that P. austrocedrae was introduced into Argentina. The aim of this study was to assess the population structure of P. austrocedrae isolates from Argentina in order to gain an understanding of the origin and spread of the pathogen. Genetic diversity was determined based on amplified fragment length polymorphisms (AFLPs). In total, 48 isolates of P. austrocedrae were obtained from infected A. chilensis trees, representing the geographical range of the host. Four primer combinations were used for the AFLP analysis. Of the 332 scored bands, 12% were polymorphic. Gene diversity (h) ranged from 0·01 to 0·03; the Shannon index (I) ranged from 0·01 to 0·04. A high degree of genetic similarity was observed among the isolates (pairwise S values = 0·958–1; 0·993 ± 0·009, mean ± SD). A frequency histogram showed that most of the isolate pairs were identical. Principal coordinate analysis using three-dimensional plots did not group any of the isolates based on their geographical origin. The low genetic diversity (within and between sites) and absence of population structure linked to geographic origin, together with the aggressiveness of the pathogen and the disease progression pattern, suggest that P. austrocedrae might have been introduced into Argentina.
Fil: Velez, Maria Laura. Centro de Investigación y Extensión Forestal Andino Patagónico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Coetzee, M. P. A.. University of Pretoria; Sudáfrica
Fil: Wingfield, M. J.. University of Pretoria; Sudáfrica
Fil: Rajchenberg, Mario. Centro de Investigación y Extensión Forestal Andino Patagónico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Greslebin, Alina Gabriela. Centro de Investigación y Extensión Forestal Andino Patagónico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Phytophthora austrocedrae is a recently discovered pathogen that causes severe mortality of Austrocedrus chilensis in Patagonia. The high level of susceptibility of the host tree, together with the distribution pattern of the pathogen, have led to the hypothesis that P. austrocedrae was introduced into Argentina. The aim of this study was to assess the population structure of P. austrocedrae isolates from Argentina in order to gain an understanding of the origin and spread of the pathogen. Genetic diversity was determined based on amplified fragment length polymorphisms (AFLPs). In total, 48 isolates of P. austrocedrae were obtained from infected A. chilensis trees, representing the geographical range of the host. Four primer combinations were used for the AFLP analysis. Of the 332 scored bands, 12% were polymorphic. Gene diversity (h) ranged from 0·01 to 0·03; the Shannon index (I) ranged from 0·01 to 0·04. A high degree of genetic similarity was observed among the isolates (pairwise S values = 0·958–1; 0·993 ± 0·009, mean ± SD). A frequency histogram showed that most of the isolate pairs were identical. Principal coordinate analysis using three-dimensional plots did not group any of the isolates based on their geographical origin. The low genetic diversity (within and between sites) and absence of population structure linked to geographic origin, together with the aggressiveness of the pathogen and the disease progression pattern, suggest that P. austrocedrae might have been introduced into Argentina.
publishDate 2014
dc.date.none.fl_str_mv 2014-02-27
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/17351
Velez, Maria Laura; Coetzee, M. P. A.; Wingfield, M. J.; Rajchenberg, Mario; Greslebin, Alina Gabriela; Evidence of low levels of genetic diversity for the Phytophthora austrocedrae population in Patagonia, Argentina; Wiley; Plant Pathology; 63; 1; 27-2-2014; 212-220
0032-0862
url http://hdl.handle.net/11336/17351
identifier_str_mv Velez, Maria Laura; Coetzee, M. P. A.; Wingfield, M. J.; Rajchenberg, Mario; Greslebin, Alina Gabriela; Evidence of low levels of genetic diversity for the Phytophthora austrocedrae population in Patagonia, Argentina; Wiley; Plant Pathology; 63; 1; 27-2-2014; 212-220
0032-0862
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/ppa.12067
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/ppa.12067/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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