Role of mincle in alveolar macrophage-dependent innate immunity against mycobacterial infections in mice

Autores
Behler, Friederike; Steinwede, Kathrin; Balboa, Luciana; Ueberberg, Bianca; Maus, Regina; Kirchhof, Gabriele; Yamasaki, Sho; Welte, Tobias; Maus, Ulrich A.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The role of macrophage-inducible C-type lectin Mincle in lung innate immunity against mycobacterial infection is incompletely defined. In this study, we show that wild-type (WT) mice responded with a delayed Mincle induction on resident alveolar macrophages and newly immigrating exudate macrophages to infection with Mycobacterium bovis bacillus Calmette-Guérin (BCG), peaking by days 14-21 posttreatment. As compared with WT mice, Mincle knockout (KO) mice exhibited decreased proinflammatory mediator responses and leukocyte recruitment upon M. bovis BCG challenge, and they demonstrated increased mycobacterial loads in pulmonary and extrapulmonary organ systems. Secondary mycobacterial infection on day 14 after primary BCG challenge led to increased cytokine gene expression in sorted alveolar macrophages of WT mice, but not Mincle KO mice, resulting in substantially reduced alveolar neutrophil recruitment and increased mycobacterial loads in the lungs of Mincle KO mice. Collectively, these data show that WT mice respond with a relatively late Mincle expression on lung sentinel cells to M. bovis BCG infection. Moreover, M. bovis BCG-induced upregulation of C-type lectin Mincle on professional phagocytes critically shapes antimycobacterial responses in both pulmonary and extrapulmonary organ systems of mice, which may be important for elucidating the role of Mincle in the control of mycobacterial dissemination in mice. Copyright © 2012 by The American Association of Immunologists, Inc.
Fil: Behler, Friederike. Hannover Medical School; Alemania
Fil: Steinwede, Kathrin. Hannover Medical School; Alemania
Fil: Balboa, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Ueberberg, Bianca. Hannover Medical School; Alemania
Fil: Maus, Regina. Hannover Medical School; Alemania
Fil: Kirchhof, Gabriele. Hannover Medical School; Alemania
Fil: Yamasaki, Sho. Kyushu University; Japón
Fil: Welte, Tobias. Hannover Medical School; Alemania
Fil: Maus, Ulrich A.. Hannover Medical School; Alemania
Materia
Mincle
BCG
Macrophages
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/52948
Acceder
id CONICETDig_b69a0e18e243d217f725f9d72a20bd21
oai_identifier_str oai:ri.conicet.gov.ar:11336/52948
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Role of mincle in alveolar macrophage-dependent innate immunity against mycobacterial infections in miceBehler, FriederikeSteinwede, KathrinBalboa, LucianaUeberberg, BiancaMaus, ReginaKirchhof, GabrieleYamasaki, ShoWelte, TobiasMaus, Ulrich A.MincleBCGMacrophageshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The role of macrophage-inducible C-type lectin Mincle in lung innate immunity against mycobacterial infection is incompletely defined. In this study, we show that wild-type (WT) mice responded with a delayed Mincle induction on resident alveolar macrophages and newly immigrating exudate macrophages to infection with Mycobacterium bovis bacillus Calmette-Guérin (BCG), peaking by days 14-21 posttreatment. As compared with WT mice, Mincle knockout (KO) mice exhibited decreased proinflammatory mediator responses and leukocyte recruitment upon M. bovis BCG challenge, and they demonstrated increased mycobacterial loads in pulmonary and extrapulmonary organ systems. Secondary mycobacterial infection on day 14 after primary BCG challenge led to increased cytokine gene expression in sorted alveolar macrophages of WT mice, but not Mincle KO mice, resulting in substantially reduced alveolar neutrophil recruitment and increased mycobacterial loads in the lungs of Mincle KO mice. Collectively, these data show that WT mice respond with a relatively late Mincle expression on lung sentinel cells to M. bovis BCG infection. Moreover, M. bovis BCG-induced upregulation of C-type lectin Mincle on professional phagocytes critically shapes antimycobacterial responses in both pulmonary and extrapulmonary organ systems of mice, which may be important for elucidating the role of Mincle in the control of mycobacterial dissemination in mice. Copyright © 2012 by The American Association of Immunologists, Inc.Fil: Behler, Friederike. Hannover Medical School; AlemaniaFil: Steinwede, Kathrin. Hannover Medical School; AlemaniaFil: Balboa, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Ueberberg, Bianca. Hannover Medical School; AlemaniaFil: Maus, Regina. Hannover Medical School; AlemaniaFil: Kirchhof, Gabriele. Hannover Medical School; AlemaniaFil: Yamasaki, Sho. Kyushu University; JapónFil: Welte, Tobias. Hannover Medical School; AlemaniaFil: Maus, Ulrich A.. Hannover Medical School; AlemaniaAmerican Association of Immunologists2012-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/52948Behler, Friederike; Steinwede, Kathrin; Balboa, Luciana; Ueberberg, Bianca; Maus, Regina; et al.; Role of mincle in alveolar macrophage-dependent innate immunity against mycobacterial infections in mice; American Association of Immunologists; Journal of Immunology; 189; 6; 9-2012; 3121-31290022-1767CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4049/jimmunol.1201399info:eu-repo/semantics/altIdentifier/url/http://www.jimmunol.org/content/189/6/3121info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:18:19Zoai:ri.conicet.gov.ar:11336/52948instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:18:19.354CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Role of mincle in alveolar macrophage-dependent innate immunity against mycobacterial infections in mice
title Role of mincle in alveolar macrophage-dependent innate immunity against mycobacterial infections in mice
spellingShingle Role of mincle in alveolar macrophage-dependent innate immunity against mycobacterial infections in mice
Behler, Friederike
Mincle
BCG
Macrophages
title_short Role of mincle in alveolar macrophage-dependent innate immunity against mycobacterial infections in mice
title_full Role of mincle in alveolar macrophage-dependent innate immunity against mycobacterial infections in mice
title_fullStr Role of mincle in alveolar macrophage-dependent innate immunity against mycobacterial infections in mice
title_full_unstemmed Role of mincle in alveolar macrophage-dependent innate immunity against mycobacterial infections in mice
title_sort Role of mincle in alveolar macrophage-dependent innate immunity against mycobacterial infections in mice
dc.creator.none.fl_str_mv Behler, Friederike
Steinwede, Kathrin
Balboa, Luciana
Ueberberg, Bianca
Maus, Regina
Kirchhof, Gabriele
Yamasaki, Sho
Welte, Tobias
Maus, Ulrich A.
author Behler, Friederike
author_facet Behler, Friederike
Steinwede, Kathrin
Balboa, Luciana
Ueberberg, Bianca
Maus, Regina
Kirchhof, Gabriele
Yamasaki, Sho
Welte, Tobias
Maus, Ulrich A.
author_role author
author2 Steinwede, Kathrin
Balboa, Luciana
Ueberberg, Bianca
Maus, Regina
Kirchhof, Gabriele
Yamasaki, Sho
Welte, Tobias
Maus, Ulrich A.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Mincle
BCG
Macrophages
topic Mincle
BCG
Macrophages
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The role of macrophage-inducible C-type lectin Mincle in lung innate immunity against mycobacterial infection is incompletely defined. In this study, we show that wild-type (WT) mice responded with a delayed Mincle induction on resident alveolar macrophages and newly immigrating exudate macrophages to infection with Mycobacterium bovis bacillus Calmette-Guérin (BCG), peaking by days 14-21 posttreatment. As compared with WT mice, Mincle knockout (KO) mice exhibited decreased proinflammatory mediator responses and leukocyte recruitment upon M. bovis BCG challenge, and they demonstrated increased mycobacterial loads in pulmonary and extrapulmonary organ systems. Secondary mycobacterial infection on day 14 after primary BCG challenge led to increased cytokine gene expression in sorted alveolar macrophages of WT mice, but not Mincle KO mice, resulting in substantially reduced alveolar neutrophil recruitment and increased mycobacterial loads in the lungs of Mincle KO mice. Collectively, these data show that WT mice respond with a relatively late Mincle expression on lung sentinel cells to M. bovis BCG infection. Moreover, M. bovis BCG-induced upregulation of C-type lectin Mincle on professional phagocytes critically shapes antimycobacterial responses in both pulmonary and extrapulmonary organ systems of mice, which may be important for elucidating the role of Mincle in the control of mycobacterial dissemination in mice. Copyright © 2012 by The American Association of Immunologists, Inc.
Fil: Behler, Friederike. Hannover Medical School; Alemania
Fil: Steinwede, Kathrin. Hannover Medical School; Alemania
Fil: Balboa, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Ueberberg, Bianca. Hannover Medical School; Alemania
Fil: Maus, Regina. Hannover Medical School; Alemania
Fil: Kirchhof, Gabriele. Hannover Medical School; Alemania
Fil: Yamasaki, Sho. Kyushu University; Japón
Fil: Welte, Tobias. Hannover Medical School; Alemania
Fil: Maus, Ulrich A.. Hannover Medical School; Alemania
description The role of macrophage-inducible C-type lectin Mincle in lung innate immunity against mycobacterial infection is incompletely defined. In this study, we show that wild-type (WT) mice responded with a delayed Mincle induction on resident alveolar macrophages and newly immigrating exudate macrophages to infection with Mycobacterium bovis bacillus Calmette-Guérin (BCG), peaking by days 14-21 posttreatment. As compared with WT mice, Mincle knockout (KO) mice exhibited decreased proinflammatory mediator responses and leukocyte recruitment upon M. bovis BCG challenge, and they demonstrated increased mycobacterial loads in pulmonary and extrapulmonary organ systems. Secondary mycobacterial infection on day 14 after primary BCG challenge led to increased cytokine gene expression in sorted alveolar macrophages of WT mice, but not Mincle KO mice, resulting in substantially reduced alveolar neutrophil recruitment and increased mycobacterial loads in the lungs of Mincle KO mice. Collectively, these data show that WT mice respond with a relatively late Mincle expression on lung sentinel cells to M. bovis BCG infection. Moreover, M. bovis BCG-induced upregulation of C-type lectin Mincle on professional phagocytes critically shapes antimycobacterial responses in both pulmonary and extrapulmonary organ systems of mice, which may be important for elucidating the role of Mincle in the control of mycobacterial dissemination in mice. Copyright © 2012 by The American Association of Immunologists, Inc.
publishDate 2012
dc.date.none.fl_str_mv 2012-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/52948
Behler, Friederike; Steinwede, Kathrin; Balboa, Luciana; Ueberberg, Bianca; Maus, Regina; et al.; Role of mincle in alveolar macrophage-dependent innate immunity against mycobacterial infections in mice; American Association of Immunologists; Journal of Immunology; 189; 6; 9-2012; 3121-3129
0022-1767
CONICET Digital
CONICET
url http://hdl.handle.net/11336/52948
identifier_str_mv Behler, Friederike; Steinwede, Kathrin; Balboa, Luciana; Ueberberg, Bianca; Maus, Regina; et al.; Role of mincle in alveolar macrophage-dependent innate immunity against mycobacterial infections in mice; American Association of Immunologists; Journal of Immunology; 189; 6; 9-2012; 3121-3129
0022-1767
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4049/jimmunol.1201399
info:eu-repo/semantics/altIdentifier/url/http://www.jimmunol.org/content/189/6/3121
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Association of Immunologists
publisher.none.fl_str_mv American Association of Immunologists
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083332190765056
score 13.22299

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