A natural antiviral and immunomodulatory compound with antiangiogenic properties

Autores
Bueno, Carlos Alberto; Lombardi, María Gabriela; Sales, María Elena; Alche, Laura Edith
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Meliacine (MA), an antiviral principle present in partially purified leaf extracts of Melia azedarach L., reduces viral load and abolishes the inflammatory reaction and neovascularization during the development of herpetic stromal keratitis in mice. 1-cinnamoyl-3,11-dihydroxymeliacarpin (CDM), obtained from MA, displays anti-herpetic and immunomodulatory activities in vitro. We investigated whether CDM interferes with the angiogenic process. CDM impeded VEGF transcription in LPS-stimulated and HSV-1-infected cells. It proved to have neither cytotoxic nor antiproliferative effect in HUVEC and to restrain HUVEC migration and formation of capillary-like tubes. Moreover, MA inhibits LMM3 tumor-induced neovascularization in vivo. We postulate that the antiangiogenic activity of CDM displayed in vitro as a consequence of their immunomodulatory properties is responsible for the antiangiogenic activity of MA in vivo, which would be associated with the lack of neovascularization in murine HSV-1-induced ocular disease. Highlights ► 1-cinnamoyl-3,11-dihydroxymeliacarpin (CDM) restrains angiogenesis in vitro. ► CDM disrupts tubule formation in vitro because it modulates cytokine production. ► CDM antiangiogenic activity explains the improvement of murine herpetic keratitis. ► This triterpenoid might be an excellent candidate as a novel anti-herpetic agent.
Fil: Bueno, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
Fil: Lombardi, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Sales, María Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Alche, Laura Edith. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
Materia
Antiviral/Inmunomodulador
Hsv-1
Antiangiogénico
Melia Azedarach L.
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/66229
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/66229
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A natural antiviral and immunomodulatory compound with antiangiogenic propertiesBueno, Carlos AlbertoLombardi, María GabrielaSales, María ElenaAlche, Laura EdithAntiviral/InmunomoduladorHsv-1AntiangiogénicoMelia Azedarach L.https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Meliacine (MA), an antiviral principle present in partially purified leaf extracts of Melia azedarach L., reduces viral load and abolishes the inflammatory reaction and neovascularization during the development of herpetic stromal keratitis in mice. 1-cinnamoyl-3,11-dihydroxymeliacarpin (CDM), obtained from MA, displays anti-herpetic and immunomodulatory activities in vitro. We investigated whether CDM interferes with the angiogenic process. CDM impeded VEGF transcription in LPS-stimulated and HSV-1-infected cells. It proved to have neither cytotoxic nor antiproliferative effect in HUVEC and to restrain HUVEC migration and formation of capillary-like tubes. Moreover, MA inhibits LMM3 tumor-induced neovascularization in vivo. We postulate that the antiangiogenic activity of CDM displayed in vitro as a consequence of their immunomodulatory properties is responsible for the antiangiogenic activity of MA in vivo, which would be associated with the lack of neovascularization in murine HSV-1-induced ocular disease. Highlights ► 1-cinnamoyl-3,11-dihydroxymeliacarpin (CDM) restrains angiogenesis in vitro. ► CDM disrupts tubule formation in vitro because it modulates cytokine production. ► CDM antiangiogenic activity explains the improvement of murine herpetic keratitis. ► This triterpenoid might be an excellent candidate as a novel anti-herpetic agent.Fil: Bueno, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; ArgentinaFil: Lombardi, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Sales, María Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Alche, Laura Edith. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; ArgentinaAcademic Press Inc Elsevier Science2012-11-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/66229Bueno, Carlos Alberto; Lombardi, María Gabriela; Sales, María Elena; Alche, Laura Edith; A natural antiviral and immunomodulatory compound with antiangiogenic properties; Academic Press Inc Elsevier Science; Microvascular Research; 84; 3; 21-11-2012; 235-2410026-2862CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.mvr.2012.09.003info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0026286212001598info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:05:46Zoai:ri.conicet.gov.ar:11336/66229instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:05:46.323CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A natural antiviral and immunomodulatory compound with antiangiogenic properties
title A natural antiviral and immunomodulatory compound with antiangiogenic properties
spellingShingle A natural antiviral and immunomodulatory compound with antiangiogenic properties
Bueno, Carlos Alberto
Antiviral/Inmunomodulador
Hsv-1
Antiangiogénico
Melia Azedarach L.
title_short A natural antiviral and immunomodulatory compound with antiangiogenic properties
title_full A natural antiviral and immunomodulatory compound with antiangiogenic properties
title_fullStr A natural antiviral and immunomodulatory compound with antiangiogenic properties
title_full_unstemmed A natural antiviral and immunomodulatory compound with antiangiogenic properties
title_sort A natural antiviral and immunomodulatory compound with antiangiogenic properties
dc.creator.none.fl_str_mv Bueno, Carlos Alberto
Lombardi, María Gabriela
Sales, María Elena
Alche, Laura Edith
author Bueno, Carlos Alberto
author_facet Bueno, Carlos Alberto
Lombardi, María Gabriela
Sales, María Elena
Alche, Laura Edith
author_role author
author2 Lombardi, María Gabriela
Sales, María Elena
Alche, Laura Edith
author2_role author
author
author
dc.subject.none.fl_str_mv Antiviral/Inmunomodulador
Hsv-1
Antiangiogénico
Melia Azedarach L.
topic Antiviral/Inmunomodulador
Hsv-1
Antiangiogénico
Melia Azedarach L.
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Meliacine (MA), an antiviral principle present in partially purified leaf extracts of Melia azedarach L., reduces viral load and abolishes the inflammatory reaction and neovascularization during the development of herpetic stromal keratitis in mice. 1-cinnamoyl-3,11-dihydroxymeliacarpin (CDM), obtained from MA, displays anti-herpetic and immunomodulatory activities in vitro. We investigated whether CDM interferes with the angiogenic process. CDM impeded VEGF transcription in LPS-stimulated and HSV-1-infected cells. It proved to have neither cytotoxic nor antiproliferative effect in HUVEC and to restrain HUVEC migration and formation of capillary-like tubes. Moreover, MA inhibits LMM3 tumor-induced neovascularization in vivo. We postulate that the antiangiogenic activity of CDM displayed in vitro as a consequence of their immunomodulatory properties is responsible for the antiangiogenic activity of MA in vivo, which would be associated with the lack of neovascularization in murine HSV-1-induced ocular disease. Highlights ► 1-cinnamoyl-3,11-dihydroxymeliacarpin (CDM) restrains angiogenesis in vitro. ► CDM disrupts tubule formation in vitro because it modulates cytokine production. ► CDM antiangiogenic activity explains the improvement of murine herpetic keratitis. ► This triterpenoid might be an excellent candidate as a novel anti-herpetic agent.
Fil: Bueno, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
Fil: Lombardi, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Sales, María Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Alche, Laura Edith. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
description Meliacine (MA), an antiviral principle present in partially purified leaf extracts of Melia azedarach L., reduces viral load and abolishes the inflammatory reaction and neovascularization during the development of herpetic stromal keratitis in mice. 1-cinnamoyl-3,11-dihydroxymeliacarpin (CDM), obtained from MA, displays anti-herpetic and immunomodulatory activities in vitro. We investigated whether CDM interferes with the angiogenic process. CDM impeded VEGF transcription in LPS-stimulated and HSV-1-infected cells. It proved to have neither cytotoxic nor antiproliferative effect in HUVEC and to restrain HUVEC migration and formation of capillary-like tubes. Moreover, MA inhibits LMM3 tumor-induced neovascularization in vivo. We postulate that the antiangiogenic activity of CDM displayed in vitro as a consequence of their immunomodulatory properties is responsible for the antiangiogenic activity of MA in vivo, which would be associated with the lack of neovascularization in murine HSV-1-induced ocular disease. Highlights ► 1-cinnamoyl-3,11-dihydroxymeliacarpin (CDM) restrains angiogenesis in vitro. ► CDM disrupts tubule formation in vitro because it modulates cytokine production. ► CDM antiangiogenic activity explains the improvement of murine herpetic keratitis. ► This triterpenoid might be an excellent candidate as a novel anti-herpetic agent.
publishDate 2012
dc.date.none.fl_str_mv 2012-11-21
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/66229
Bueno, Carlos Alberto; Lombardi, María Gabriela; Sales, María Elena; Alche, Laura Edith; A natural antiviral and immunomodulatory compound with antiangiogenic properties; Academic Press Inc Elsevier Science; Microvascular Research; 84; 3; 21-11-2012; 235-241
0026-2862
CONICET Digital
CONICET
url http://hdl.handle.net/11336/66229
identifier_str_mv Bueno, Carlos Alberto; Lombardi, María Gabriela; Sales, María Elena; Alche, Laura Edith; A natural antiviral and immunomodulatory compound with antiangiogenic properties; Academic Press Inc Elsevier Science; Microvascular Research; 84; 3; 21-11-2012; 235-241
0026-2862
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.mvr.2012.09.003
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0026286212001598
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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