Genetically attenuated Trypanosoma cruzi parasites as a potential vaccination tool
- Autores
- Pérez Brandan, Cecilia María; Basombrio, Miguel Ángel Manuel
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Chagas disease is the clinical manifestation of the infection produced by the parasite Trypanosoma cruzi. Currently there is no vaccine to prevent this disease and the protection attained with vaccines containing non-replicating parasites is limited. Genetically attenuated trypanosomatid parasites can be obtained by deletion of selected genes. Gene deletion takes advantage of the fact that this parasite can undergo homologous recombination between endogenous and foreign DNA sequences artificially introduced in the cells. This approach facilitated the discovery of several unknown gene functions, as well as allowing us to speculate about the potential for genetically attenuated live organisms as experimental immunogens. Vaccination with live attenuated parasites has been used effectively in mice to reduce parasitemia and histological damage, and in dogs, to prevent vector-delivered infection in the field. However, the use of live parasites as immunogens is controversial due to the risk of reversion to a virulent phenotype. Herein, we present our results from experiments on genetic manipulation of two T. cruzi strains to produce parasites with impaired replication and infectivity, and using the mutation of the dhfr-ts gene as a safety device against reversion to virulence.
Fil: Pérez Brandan, Cecilia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Basombrio, Miguel Ángel Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina - Materia
-
Trypanosoma Cruzi
Gene Knockout
Vaccine
Chagas Disease
Dhfr-Ts - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/24329
Ver los metadatos del registro completo
id |
CONICETDig_b4b16a058c0b588608914f20d8f8a580 |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/24329 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Genetically attenuated Trypanosoma cruzi parasites as a potential vaccination toolPérez Brandan, Cecilia MaríaBasombrio, Miguel Ángel ManuelTrypanosoma CruziGene KnockoutVaccineChagas DiseaseDhfr-Tshttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Chagas disease is the clinical manifestation of the infection produced by the parasite Trypanosoma cruzi. Currently there is no vaccine to prevent this disease and the protection attained with vaccines containing non-replicating parasites is limited. Genetically attenuated trypanosomatid parasites can be obtained by deletion of selected genes. Gene deletion takes advantage of the fact that this parasite can undergo homologous recombination between endogenous and foreign DNA sequences artificially introduced in the cells. This approach facilitated the discovery of several unknown gene functions, as well as allowing us to speculate about the potential for genetically attenuated live organisms as experimental immunogens. Vaccination with live attenuated parasites has been used effectively in mice to reduce parasitemia and histological damage, and in dogs, to prevent vector-delivered infection in the field. However, the use of live parasites as immunogens is controversial due to the risk of reversion to a virulent phenotype. Herein, we present our results from experiments on genetic manipulation of two T. cruzi strains to produce parasites with impaired replication and infectivity, and using the mutation of the dhfr-ts gene as a safety device against reversion to virulence.Fil: Pérez Brandan, Cecilia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Basombrio, Miguel Ángel Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaTaylor & Francis2012-06-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24329Pérez Brandan, Cecilia María; Basombrio, Miguel Ángel Manuel; Genetically attenuated Trypanosoma cruzi parasites as a potential vaccination tool; Taylor & Francis; Bioengineered; 3; 4; 18-6-2012; 1-51949-1018CONICET DigitalCONICETenginfo:eu-repo/semantics/reference/url/http://www.tandfonline.com/doi/abs/10.4161/bioe.20680info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/full/10.4161/bioe.20680info:eu-repo/semantics/altIdentifier/doi/10.4161/bioe.20680info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:43:34Zoai:ri.conicet.gov.ar:11336/24329instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:43:35.0CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Genetically attenuated Trypanosoma cruzi parasites as a potential vaccination tool |
title |
Genetically attenuated Trypanosoma cruzi parasites as a potential vaccination tool |
spellingShingle |
Genetically attenuated Trypanosoma cruzi parasites as a potential vaccination tool Pérez Brandan, Cecilia María Trypanosoma Cruzi Gene Knockout Vaccine Chagas Disease Dhfr-Ts |
title_short |
Genetically attenuated Trypanosoma cruzi parasites as a potential vaccination tool |
title_full |
Genetically attenuated Trypanosoma cruzi parasites as a potential vaccination tool |
title_fullStr |
Genetically attenuated Trypanosoma cruzi parasites as a potential vaccination tool |
title_full_unstemmed |
Genetically attenuated Trypanosoma cruzi parasites as a potential vaccination tool |
title_sort |
Genetically attenuated Trypanosoma cruzi parasites as a potential vaccination tool |
dc.creator.none.fl_str_mv |
Pérez Brandan, Cecilia María Basombrio, Miguel Ángel Manuel |
author |
Pérez Brandan, Cecilia María |
author_facet |
Pérez Brandan, Cecilia María Basombrio, Miguel Ángel Manuel |
author_role |
author |
author2 |
Basombrio, Miguel Ángel Manuel |
author2_role |
author |
dc.subject.none.fl_str_mv |
Trypanosoma Cruzi Gene Knockout Vaccine Chagas Disease Dhfr-Ts |
topic |
Trypanosoma Cruzi Gene Knockout Vaccine Chagas Disease Dhfr-Ts |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Chagas disease is the clinical manifestation of the infection produced by the parasite Trypanosoma cruzi. Currently there is no vaccine to prevent this disease and the protection attained with vaccines containing non-replicating parasites is limited. Genetically attenuated trypanosomatid parasites can be obtained by deletion of selected genes. Gene deletion takes advantage of the fact that this parasite can undergo homologous recombination between endogenous and foreign DNA sequences artificially introduced in the cells. This approach facilitated the discovery of several unknown gene functions, as well as allowing us to speculate about the potential for genetically attenuated live organisms as experimental immunogens. Vaccination with live attenuated parasites has been used effectively in mice to reduce parasitemia and histological damage, and in dogs, to prevent vector-delivered infection in the field. However, the use of live parasites as immunogens is controversial due to the risk of reversion to a virulent phenotype. Herein, we present our results from experiments on genetic manipulation of two T. cruzi strains to produce parasites with impaired replication and infectivity, and using the mutation of the dhfr-ts gene as a safety device against reversion to virulence. Fil: Pérez Brandan, Cecilia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina Fil: Basombrio, Miguel Ángel Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina |
description |
Chagas disease is the clinical manifestation of the infection produced by the parasite Trypanosoma cruzi. Currently there is no vaccine to prevent this disease and the protection attained with vaccines containing non-replicating parasites is limited. Genetically attenuated trypanosomatid parasites can be obtained by deletion of selected genes. Gene deletion takes advantage of the fact that this parasite can undergo homologous recombination between endogenous and foreign DNA sequences artificially introduced in the cells. This approach facilitated the discovery of several unknown gene functions, as well as allowing us to speculate about the potential for genetically attenuated live organisms as experimental immunogens. Vaccination with live attenuated parasites has been used effectively in mice to reduce parasitemia and histological damage, and in dogs, to prevent vector-delivered infection in the field. However, the use of live parasites as immunogens is controversial due to the risk of reversion to a virulent phenotype. Herein, we present our results from experiments on genetic manipulation of two T. cruzi strains to produce parasites with impaired replication and infectivity, and using the mutation of the dhfr-ts gene as a safety device against reversion to virulence. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-06-18 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/24329 Pérez Brandan, Cecilia María; Basombrio, Miguel Ángel Manuel; Genetically attenuated Trypanosoma cruzi parasites as a potential vaccination tool; Taylor & Francis; Bioengineered; 3; 4; 18-6-2012; 1-5 1949-1018 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/24329 |
identifier_str_mv |
Pérez Brandan, Cecilia María; Basombrio, Miguel Ángel Manuel; Genetically attenuated Trypanosoma cruzi parasites as a potential vaccination tool; Taylor & Francis; Bioengineered; 3; 4; 18-6-2012; 1-5 1949-1018 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/reference/url/http://www.tandfonline.com/doi/abs/10.4161/bioe.20680 info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/full/10.4161/bioe.20680 info:eu-repo/semantics/altIdentifier/doi/10.4161/bioe.20680 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Taylor & Francis |
publisher.none.fl_str_mv |
Taylor & Francis |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844614471359660032 |
score |
13.070432 |