Evaluation of pathogen P21 protein as a potential modulator of the protective immunity induced by trypanosoma cruzi attenuated parasites

Autores
Pérez Brandan, Cecilia María; Mesias, Andrea Cecilia; Acuña, Leonardo; Teixeira, Thaise Lara; Silva, Claudio Vieira da
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
TcP21 is a ubiquitous secreted protein of Trypanosoma cruzi and its recombinant form (rP21) promotes parasite cell invasion and acts as a phagocytosis inducer by activating actin polymerization in the host cell. Our goal was to evaluate if the additional supplementation of rP21 during a prime/boost/challenge scheme with T.cruzi TCC attenuated parasites could modify the well known protective behavior conferred by these parasites. The humoral immune response was evaluated through the assessment of total anti-T. cruzi antibodies as well as IgG subtypes. IFN-, TNF- and IL-10 were measured in supernatants of splenic cells stimulated with total parasite homogenate or rP21. Our results demonstrated that, when comparing TCC+ rP21 vs. TCC vaccinated animals, the levels of IFN- were significantly higher in the former group, while the levels of IL-10 and TNF- were significantly lower. Further, the measurement of parasite load after lethal challenge showed an exacerbated infection and parasite load in heart and skeletal muscle after pretreatmentwith rP21, suggesting the important role of this protein during parasite natural invasion process. In conclusion, our results demonstrated that rP21 may have adjuvant capacity able to modify the cytokine immune profile elicited by attenuated parasites.
Fil: Pérez Brandan, Cecilia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Mesias, Andrea Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Acuña, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Teixeira, Thaise Lara. Universidade Federal de Uberlândia; Brasil
Fil: Silva, Claudio Vieira da. Universidade Federal de Uberlândia; Brasil
Materia
ATTENUATED VACCINE
CHAGAS DISEASE
P21
TRYPANOSOMA CRUZI
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/120540
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of pathogen P21 protein as a potential modulator of the protective immunity induced by trypanosoma cruzi attenuated parasitesPérez Brandan, Cecilia MaríaMesias, Andrea CeciliaAcuña, LeonardoTeixeira, Thaise LaraSilva, Claudio Vieira daATTENUATED VACCINECHAGAS DISEASEP21TRYPANOSOMA CRUZIhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3TcP21 is a ubiquitous secreted protein of Trypanosoma cruzi and its recombinant form (rP21) promotes parasite cell invasion and acts as a phagocytosis inducer by activating actin polymerization in the host cell. Our goal was to evaluate if the additional supplementation of rP21 during a prime/boost/challenge scheme with T.cruzi TCC attenuated parasites could modify the well known protective behavior conferred by these parasites. The humoral immune response was evaluated through the assessment of total anti-T. cruzi antibodies as well as IgG subtypes. IFN-, TNF- and IL-10 were measured in supernatants of splenic cells stimulated with total parasite homogenate or rP21. Our results demonstrated that, when comparing TCC+ rP21 vs. TCC vaccinated animals, the levels of IFN- were significantly higher in the former group, while the levels of IL-10 and TNF- were significantly lower. Further, the measurement of parasite load after lethal challenge showed an exacerbated infection and parasite load in heart and skeletal muscle after pretreatmentwith rP21, suggesting the important role of this protein during parasite natural invasion process. In conclusion, our results demonstrated that rP21 may have adjuvant capacity able to modify the cytokine immune profile elicited by attenuated parasites.Fil: Pérez Brandan, Cecilia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Mesias, Andrea Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Acuña, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Teixeira, Thaise Lara. Universidade Federal de Uberlândia; BrasilFil: Silva, Claudio Vieira da. Universidade Federal de Uberlândia; BrasilFundação Oswaldo Cruz2019-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/120540Pérez Brandan, Cecilia María; Mesias, Andrea Cecilia; Acuña, Leonardo; Teixeira, Thaise Lara; Silva, Claudio Vieira da; Evaluation of pathogen P21 protein as a potential modulator of the protective immunity induced by trypanosoma cruzi attenuated parasites; Fundação Oswaldo Cruz; Memórias do Instituto Oswaldo Cruz; 114; 4; 4-2019; 1-70074-02761678-8060CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1590/0074-02760180571info:eu-repo/semantics/altIdentifier/url/https://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762019000100325&tlng=eninfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:42:43Zoai:ri.conicet.gov.ar:11336/120540instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:42:43.736CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of pathogen P21 protein as a potential modulator of the protective immunity induced by trypanosoma cruzi attenuated parasites
title Evaluation of pathogen P21 protein as a potential modulator of the protective immunity induced by trypanosoma cruzi attenuated parasites
spellingShingle Evaluation of pathogen P21 protein as a potential modulator of the protective immunity induced by trypanosoma cruzi attenuated parasites
Pérez Brandan, Cecilia María
ATTENUATED VACCINE
CHAGAS DISEASE
P21
TRYPANOSOMA CRUZI
title_short Evaluation of pathogen P21 protein as a potential modulator of the protective immunity induced by trypanosoma cruzi attenuated parasites
title_full Evaluation of pathogen P21 protein as a potential modulator of the protective immunity induced by trypanosoma cruzi attenuated parasites
title_fullStr Evaluation of pathogen P21 protein as a potential modulator of the protective immunity induced by trypanosoma cruzi attenuated parasites
title_full_unstemmed Evaluation of pathogen P21 protein as a potential modulator of the protective immunity induced by trypanosoma cruzi attenuated parasites
title_sort Evaluation of pathogen P21 protein as a potential modulator of the protective immunity induced by trypanosoma cruzi attenuated parasites
dc.creator.none.fl_str_mv Pérez Brandan, Cecilia María
Mesias, Andrea Cecilia
Acuña, Leonardo
Teixeira, Thaise Lara
Silva, Claudio Vieira da
author Pérez Brandan, Cecilia María
author_facet Pérez Brandan, Cecilia María
Mesias, Andrea Cecilia
Acuña, Leonardo
Teixeira, Thaise Lara
Silva, Claudio Vieira da
author_role author
author2 Mesias, Andrea Cecilia
Acuña, Leonardo
Teixeira, Thaise Lara
Silva, Claudio Vieira da
author2_role author
author
author
author
dc.subject.none.fl_str_mv ATTENUATED VACCINE
CHAGAS DISEASE
P21
TRYPANOSOMA CRUZI
topic ATTENUATED VACCINE
CHAGAS DISEASE
P21
TRYPANOSOMA CRUZI
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv TcP21 is a ubiquitous secreted protein of Trypanosoma cruzi and its recombinant form (rP21) promotes parasite cell invasion and acts as a phagocytosis inducer by activating actin polymerization in the host cell. Our goal was to evaluate if the additional supplementation of rP21 during a prime/boost/challenge scheme with T.cruzi TCC attenuated parasites could modify the well known protective behavior conferred by these parasites. The humoral immune response was evaluated through the assessment of total anti-T. cruzi antibodies as well as IgG subtypes. IFN-, TNF- and IL-10 were measured in supernatants of splenic cells stimulated with total parasite homogenate or rP21. Our results demonstrated that, when comparing TCC+ rP21 vs. TCC vaccinated animals, the levels of IFN- were significantly higher in the former group, while the levels of IL-10 and TNF- were significantly lower. Further, the measurement of parasite load after lethal challenge showed an exacerbated infection and parasite load in heart and skeletal muscle after pretreatmentwith rP21, suggesting the important role of this protein during parasite natural invasion process. In conclusion, our results demonstrated that rP21 may have adjuvant capacity able to modify the cytokine immune profile elicited by attenuated parasites.
Fil: Pérez Brandan, Cecilia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Mesias, Andrea Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Acuña, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Teixeira, Thaise Lara. Universidade Federal de Uberlândia; Brasil
Fil: Silva, Claudio Vieira da. Universidade Federal de Uberlândia; Brasil
description TcP21 is a ubiquitous secreted protein of Trypanosoma cruzi and its recombinant form (rP21) promotes parasite cell invasion and acts as a phagocytosis inducer by activating actin polymerization in the host cell. Our goal was to evaluate if the additional supplementation of rP21 during a prime/boost/challenge scheme with T.cruzi TCC attenuated parasites could modify the well known protective behavior conferred by these parasites. The humoral immune response was evaluated through the assessment of total anti-T. cruzi antibodies as well as IgG subtypes. IFN-, TNF- and IL-10 were measured in supernatants of splenic cells stimulated with total parasite homogenate or rP21. Our results demonstrated that, when comparing TCC+ rP21 vs. TCC vaccinated animals, the levels of IFN- were significantly higher in the former group, while the levels of IL-10 and TNF- were significantly lower. Further, the measurement of parasite load after lethal challenge showed an exacerbated infection and parasite load in heart and skeletal muscle after pretreatmentwith rP21, suggesting the important role of this protein during parasite natural invasion process. In conclusion, our results demonstrated that rP21 may have adjuvant capacity able to modify the cytokine immune profile elicited by attenuated parasites.
publishDate 2019
dc.date.none.fl_str_mv 2019-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/120540
Pérez Brandan, Cecilia María; Mesias, Andrea Cecilia; Acuña, Leonardo; Teixeira, Thaise Lara; Silva, Claudio Vieira da; Evaluation of pathogen P21 protein as a potential modulator of the protective immunity induced by trypanosoma cruzi attenuated parasites; Fundação Oswaldo Cruz; Memórias do Instituto Oswaldo Cruz; 114; 4; 4-2019; 1-7
0074-0276
1678-8060
CONICET Digital
CONICET
url http://hdl.handle.net/11336/120540
identifier_str_mv Pérez Brandan, Cecilia María; Mesias, Andrea Cecilia; Acuña, Leonardo; Teixeira, Thaise Lara; Silva, Claudio Vieira da; Evaluation of pathogen P21 protein as a potential modulator of the protective immunity induced by trypanosoma cruzi attenuated parasites; Fundação Oswaldo Cruz; Memórias do Instituto Oswaldo Cruz; 114; 4; 4-2019; 1-7
0074-0276
1678-8060
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1590/0074-02760180571
info:eu-repo/semantics/altIdentifier/url/https://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762019000100325&tlng=en
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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