Chemometrics-assisted simple UV-spectroscopic determination of carbamazepine in human serum and comparison with reference methods
- Autores
- Cámara, María Silvia; Mastandrea, Carlos Raúl; Goicoechea, Hector Casimiro
- Año de publicación
- 2005
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In the present report, CBZ is determined on real patients samples by using a procedure completely assisted by chemometric tools. Firstly, a response surface methodology based on a mixture design was applied in order to select the best conditions in the extraction step. Finally, multivariate calibration PLS-1 was applied to second derivative UV spectra eliminating a shift baseline effect originated in the extraction procedure. The performance assessment included: a) a three level precision study, b) a recovery study analysing spiked samples, and c) method comparison with HPLC and FPIA methods applied on real patient samples. The results obtained show the potentiality of the presently studied methodology for the following of patients treated with this anticonvulsivant.
Fil: Cámara, María Silvia. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Mastandrea, Carlos Raúl. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina - Materia
-
CARBAMAZEPINE
SERUM SAMPLES
CHEMOMETRICS
PARTIAL LEAST SQUARES REGRESSION - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/102674
Ver los metadatos del registro completo
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Chemometrics-assisted simple UV-spectroscopic determination of carbamazepine in human serum and comparison with reference methodsCámara, María SilviaMastandrea, Carlos RaúlGoicoechea, Hector CasimiroCARBAMAZEPINESERUM SAMPLESCHEMOMETRICSPARTIAL LEAST SQUARES REGRESSIONhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1In the present report, CBZ is determined on real patients samples by using a procedure completely assisted by chemometric tools. Firstly, a response surface methodology based on a mixture design was applied in order to select the best conditions in the extraction step. Finally, multivariate calibration PLS-1 was applied to second derivative UV spectra eliminating a shift baseline effect originated in the extraction procedure. The performance assessment included: a) a three level precision study, b) a recovery study analysing spiked samples, and c) method comparison with HPLC and FPIA methods applied on real patient samples. The results obtained show the potentiality of the presently studied methodology for the following of patients treated with this anticonvulsivant.Fil: Cámara, María Silvia. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Mastandrea, Carlos Raúl. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaElsevier Science2005-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/102674Cámara, María Silvia; Mastandrea, Carlos Raúl; Goicoechea, Hector Casimiro; Chemometrics-assisted simple UV-spectroscopic determination of carbamazepine in human serum and comparison with reference methods; Elsevier Science; Journal Of Biochemical And Biophysical Methods; 64; 9-2005; 153-1660165-022XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0165022X05001119info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jbbm.2005.07.001info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:09:51Zoai:ri.conicet.gov.ar:11336/102674instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:09:51.785CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Chemometrics-assisted simple UV-spectroscopic determination of carbamazepine in human serum and comparison with reference methods |
title |
Chemometrics-assisted simple UV-spectroscopic determination of carbamazepine in human serum and comparison with reference methods |
spellingShingle |
Chemometrics-assisted simple UV-spectroscopic determination of carbamazepine in human serum and comparison with reference methods Cámara, María Silvia CARBAMAZEPINE SERUM SAMPLES CHEMOMETRICS PARTIAL LEAST SQUARES REGRESSION |
title_short |
Chemometrics-assisted simple UV-spectroscopic determination of carbamazepine in human serum and comparison with reference methods |
title_full |
Chemometrics-assisted simple UV-spectroscopic determination of carbamazepine in human serum and comparison with reference methods |
title_fullStr |
Chemometrics-assisted simple UV-spectroscopic determination of carbamazepine in human serum and comparison with reference methods |
title_full_unstemmed |
Chemometrics-assisted simple UV-spectroscopic determination of carbamazepine in human serum and comparison with reference methods |
title_sort |
Chemometrics-assisted simple UV-spectroscopic determination of carbamazepine in human serum and comparison with reference methods |
dc.creator.none.fl_str_mv |
Cámara, María Silvia Mastandrea, Carlos Raúl Goicoechea, Hector Casimiro |
author |
Cámara, María Silvia |
author_facet |
Cámara, María Silvia Mastandrea, Carlos Raúl Goicoechea, Hector Casimiro |
author_role |
author |
author2 |
Mastandrea, Carlos Raúl Goicoechea, Hector Casimiro |
author2_role |
author author |
dc.subject.none.fl_str_mv |
CARBAMAZEPINE SERUM SAMPLES CHEMOMETRICS PARTIAL LEAST SQUARES REGRESSION |
topic |
CARBAMAZEPINE SERUM SAMPLES CHEMOMETRICS PARTIAL LEAST SQUARES REGRESSION |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
In the present report, CBZ is determined on real patients samples by using a procedure completely assisted by chemometric tools. Firstly, a response surface methodology based on a mixture design was applied in order to select the best conditions in the extraction step. Finally, multivariate calibration PLS-1 was applied to second derivative UV spectra eliminating a shift baseline effect originated in the extraction procedure. The performance assessment included: a) a three level precision study, b) a recovery study analysing spiked samples, and c) method comparison with HPLC and FPIA methods applied on real patient samples. The results obtained show the potentiality of the presently studied methodology for the following of patients treated with this anticonvulsivant. Fil: Cámara, María Silvia. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina Fil: Mastandrea, Carlos Raúl. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina Fil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina |
description |
In the present report, CBZ is determined on real patients samples by using a procedure completely assisted by chemometric tools. Firstly, a response surface methodology based on a mixture design was applied in order to select the best conditions in the extraction step. Finally, multivariate calibration PLS-1 was applied to second derivative UV spectra eliminating a shift baseline effect originated in the extraction procedure. The performance assessment included: a) a three level precision study, b) a recovery study analysing spiked samples, and c) method comparison with HPLC and FPIA methods applied on real patient samples. The results obtained show the potentiality of the presently studied methodology for the following of patients treated with this anticonvulsivant. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-09 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/102674 Cámara, María Silvia; Mastandrea, Carlos Raúl; Goicoechea, Hector Casimiro; Chemometrics-assisted simple UV-spectroscopic determination of carbamazepine in human serum and comparison with reference methods; Elsevier Science; Journal Of Biochemical And Biophysical Methods; 64; 9-2005; 153-166 0165-022X CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/102674 |
identifier_str_mv |
Cámara, María Silvia; Mastandrea, Carlos Raúl; Goicoechea, Hector Casimiro; Chemometrics-assisted simple UV-spectroscopic determination of carbamazepine in human serum and comparison with reference methods; Elsevier Science; Journal Of Biochemical And Biophysical Methods; 64; 9-2005; 153-166 0165-022X CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0165022X05001119 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jbbm.2005.07.001 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science |
publisher.none.fl_str_mv |
Elsevier Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |