Chemometrics-assisted simple UV-spectroscopic determination of carbamazepine in human serum and comparison with reference methods

Autores
Cámara, María Silvia; Mastandrea, Carlos Raúl; Goicoechea, Hector Casimiro
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the present report, CBZ is determined on real patients samples by using a procedure completely assisted by chemometric tools. Firstly, a response surface methodology based on a mixture design was applied in order to select the best conditions in the extraction step. Finally, multivariate calibration PLS-1 was applied to second derivative UV spectra eliminating a shift baseline effect originated in the extraction procedure. The performance assessment included: a) a three level precision study, b) a recovery study analysing spiked samples, and c) method comparison with HPLC and FPIA methods applied on real patient samples. The results obtained show the potentiality of the presently studied methodology for the following of patients treated with this anticonvulsivant.
Fil: Cámara, María Silvia. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Mastandrea, Carlos Raúl. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Materia
CARBAMAZEPINE
SERUM SAMPLES
CHEMOMETRICS
PARTIAL LEAST SQUARES REGRESSION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/102674
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Chemometrics-assisted simple UV-spectroscopic determination of carbamazepine in human serum and comparison with reference methodsCámara, María SilviaMastandrea, Carlos RaúlGoicoechea, Hector CasimiroCARBAMAZEPINESERUM SAMPLESCHEMOMETRICSPARTIAL LEAST SQUARES REGRESSIONhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1In the present report, CBZ is determined on real patients samples by using a procedure completely assisted by chemometric tools. Firstly, a response surface methodology based on a mixture design was applied in order to select the best conditions in the extraction step. Finally, multivariate calibration PLS-1 was applied to second derivative UV spectra eliminating a shift baseline effect originated in the extraction procedure. The performance assessment included: a) a three level precision study, b) a recovery study analysing spiked samples, and c) method comparison with HPLC and FPIA methods applied on real patient samples. The results obtained show the potentiality of the presently studied methodology for the following of patients treated with this anticonvulsivant.Fil: Cámara, María Silvia. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Mastandrea, Carlos Raúl. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaElsevier Science2005-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/102674Cámara, María Silvia; Mastandrea, Carlos Raúl; Goicoechea, Hector Casimiro; Chemometrics-assisted simple UV-spectroscopic determination of carbamazepine in human serum and comparison with reference methods; Elsevier Science; Journal Of Biochemical And Biophysical Methods; 64; 9-2005; 153-1660165-022XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0165022X05001119info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jbbm.2005.07.001info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:39:47Zoai:ri.conicet.gov.ar:11336/102674instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:39:47.437CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Chemometrics-assisted simple UV-spectroscopic determination of carbamazepine in human serum and comparison with reference methods
title Chemometrics-assisted simple UV-spectroscopic determination of carbamazepine in human serum and comparison with reference methods
spellingShingle Chemometrics-assisted simple UV-spectroscopic determination of carbamazepine in human serum and comparison with reference methods
Cámara, María Silvia
CARBAMAZEPINE
SERUM SAMPLES
CHEMOMETRICS
PARTIAL LEAST SQUARES REGRESSION
title_short Chemometrics-assisted simple UV-spectroscopic determination of carbamazepine in human serum and comparison with reference methods
title_full Chemometrics-assisted simple UV-spectroscopic determination of carbamazepine in human serum and comparison with reference methods
title_fullStr Chemometrics-assisted simple UV-spectroscopic determination of carbamazepine in human serum and comparison with reference methods
title_full_unstemmed Chemometrics-assisted simple UV-spectroscopic determination of carbamazepine in human serum and comparison with reference methods
title_sort Chemometrics-assisted simple UV-spectroscopic determination of carbamazepine in human serum and comparison with reference methods
dc.creator.none.fl_str_mv Cámara, María Silvia
Mastandrea, Carlos Raúl
Goicoechea, Hector Casimiro
author Cámara, María Silvia
author_facet Cámara, María Silvia
Mastandrea, Carlos Raúl
Goicoechea, Hector Casimiro
author_role author
author2 Mastandrea, Carlos Raúl
Goicoechea, Hector Casimiro
author2_role author
author
dc.subject.none.fl_str_mv CARBAMAZEPINE
SERUM SAMPLES
CHEMOMETRICS
PARTIAL LEAST SQUARES REGRESSION
topic CARBAMAZEPINE
SERUM SAMPLES
CHEMOMETRICS
PARTIAL LEAST SQUARES REGRESSION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In the present report, CBZ is determined on real patients samples by using a procedure completely assisted by chemometric tools. Firstly, a response surface methodology based on a mixture design was applied in order to select the best conditions in the extraction step. Finally, multivariate calibration PLS-1 was applied to second derivative UV spectra eliminating a shift baseline effect originated in the extraction procedure. The performance assessment included: a) a three level precision study, b) a recovery study analysing spiked samples, and c) method comparison with HPLC and FPIA methods applied on real patient samples. The results obtained show the potentiality of the presently studied methodology for the following of patients treated with this anticonvulsivant.
Fil: Cámara, María Silvia. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Mastandrea, Carlos Raúl. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
description In the present report, CBZ is determined on real patients samples by using a procedure completely assisted by chemometric tools. Firstly, a response surface methodology based on a mixture design was applied in order to select the best conditions in the extraction step. Finally, multivariate calibration PLS-1 was applied to second derivative UV spectra eliminating a shift baseline effect originated in the extraction procedure. The performance assessment included: a) a three level precision study, b) a recovery study analysing spiked samples, and c) method comparison with HPLC and FPIA methods applied on real patient samples. The results obtained show the potentiality of the presently studied methodology for the following of patients treated with this anticonvulsivant.
publishDate 2005
dc.date.none.fl_str_mv 2005-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/102674
Cámara, María Silvia; Mastandrea, Carlos Raúl; Goicoechea, Hector Casimiro; Chemometrics-assisted simple UV-spectroscopic determination of carbamazepine in human serum and comparison with reference methods; Elsevier Science; Journal Of Biochemical And Biophysical Methods; 64; 9-2005; 153-166
0165-022X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/102674
identifier_str_mv Cámara, María Silvia; Mastandrea, Carlos Raúl; Goicoechea, Hector Casimiro; Chemometrics-assisted simple UV-spectroscopic determination of carbamazepine in human serum and comparison with reference methods; Elsevier Science; Journal Of Biochemical And Biophysical Methods; 64; 9-2005; 153-166
0165-022X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0165022X05001119
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jbbm.2005.07.001
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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