The metabotropic glutamate receptor homologs MGL-1 and MGL-2 are key for sensing nutritional status in C. elegans

Autores
Lacour, Ailin; Blanco, Maria Gabriela; Zabala, Agustina; de Rosa, Maria Jose; Rayes, Diego Hernán
Año de publicación
2022
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The mechanisms that allow the nervous system to sense nutritional state and adapt animal behavior are poorly understood in most species. The simplicity of its NS and its known connectome make C. elegans a useful system to study these mechanisms. Results from our laboratory showed that inhibition of the tyraminergic neuron RIM during fasting, enhances serotonin release from other neurons when the animal reencounters food, allowing it to slow down locomotion and start feeding. Mutations in the GPCRs, MGL-1 and MGL-2, located in two presynaptic interneurons to RIM have been reported to induce autophagy even in well-fed animals. Here, we performed behavioral assays on mgl-1; mgl-2 mutants. We found that these animals, even when well fed, show a significant decrease in locomotion when they find food similar to fasted wild-type animals. Moreover, when we exposed these mutants to GFP-expressing bacteria, the fluorescence in the intestine is higher than that of wild-type animals, suggesting a higher feeding rate. These initial results suggest that the metabotropic receptors MGL-1 and MGL-2 are key for C. elegans to censor satiation molecules. We propose, therefore, to determine what these satiety signals are and the neuronal circuits involved. Given that this behavioral plasticity modulated by the nutritional state is observed throughout the animal kingdom, and that several fundamental processes are highly conserved, these results may provide universally relevant information
Fil: Lacour, Ailin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Blanco, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Zabala, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: de Rosa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Rayes, Diego Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Sociedad Argentina de Neurociencias Meeting (SAN2022)
Buenos Aires
Argentina
Sociedad Argentina de Neurociencias
Materia
C. ELEGANS
SATIATION
NERVOUS CIRCUITS
FEEDING BEHAVIOR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/229784
Acceder
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network_name_str CONICET Digital (CONICET)
spelling The metabotropic glutamate receptor homologs MGL-1 and MGL-2 are key for sensing nutritional status in C. elegansLacour, AilinBlanco, Maria GabrielaZabala, Agustinade Rosa, Maria JoseRayes, Diego HernánC. ELEGANSSATIATIONNERVOUS CIRCUITSFEEDING BEHAVIORhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The mechanisms that allow the nervous system to sense nutritional state and adapt animal behavior are poorly understood in most species. The simplicity of its NS and its known connectome make C. elegans a useful system to study these mechanisms. Results from our laboratory showed that inhibition of the tyraminergic neuron RIM during fasting, enhances serotonin release from other neurons when the animal reencounters food, allowing it to slow down locomotion and start feeding. Mutations in the GPCRs, MGL-1 and MGL-2, located in two presynaptic interneurons to RIM have been reported to induce autophagy even in well-fed animals. Here, we performed behavioral assays on mgl-1; mgl-2 mutants. We found that these animals, even when well fed, show a significant decrease in locomotion when they find food similar to fasted wild-type animals. Moreover, when we exposed these mutants to GFP-expressing bacteria, the fluorescence in the intestine is higher than that of wild-type animals, suggesting a higher feeding rate. These initial results suggest that the metabotropic receptors MGL-1 and MGL-2 are key for C. elegans to censor satiation molecules. We propose, therefore, to determine what these satiety signals are and the neuronal circuits involved. Given that this behavioral plasticity modulated by the nutritional state is observed throughout the animal kingdom, and that several fundamental processes are highly conserved, these results may provide universally relevant informationFil: Lacour, Ailin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Blanco, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Zabala, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: de Rosa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Rayes, Diego Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaSociedad Argentina de Neurociencias Meeting (SAN2022)Buenos AiresArgentinaSociedad Argentina de NeurocienciasSociedad Argentina de Neurociencias2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/229784The metabotropic glutamate receptor homologs MGL-1 and MGL-2 are key for sensing nutritional status in C. elegans; Sociedad Argentina de Neurociencias Meeting (SAN2022); Buenos Aires; Argentina; 2022; 1-1CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://csan2022.saneurociencias.org.ar/index.php/2022/09/23/214-effect-of-a-ketogenic-diet-on-the-expression-of-potassium-channels-controlling-neuronal-excitability/index.htmlinfo:eu-repo/semantics/altIdentifier/url/https://csan2022.saneurociencias.org.ar/index.php/2022/09/23/182-the-metabotropic-glutamate-receptor-homologs-mgl-1-and-mgl-2-are-key-for-sensing-nutritional-status-in-c-elegans/index.htmlNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:09:44Zoai:ri.conicet.gov.ar:11336/229784instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:09:44.4CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The metabotropic glutamate receptor homologs MGL-1 and MGL-2 are key for sensing nutritional status in C. elegans
title The metabotropic glutamate receptor homologs MGL-1 and MGL-2 are key for sensing nutritional status in C. elegans
spellingShingle The metabotropic glutamate receptor homologs MGL-1 and MGL-2 are key for sensing nutritional status in C. elegans
Lacour, Ailin
C. ELEGANS
SATIATION
NERVOUS CIRCUITS
FEEDING BEHAVIOR
title_short The metabotropic glutamate receptor homologs MGL-1 and MGL-2 are key for sensing nutritional status in C. elegans
title_full The metabotropic glutamate receptor homologs MGL-1 and MGL-2 are key for sensing nutritional status in C. elegans
title_fullStr The metabotropic glutamate receptor homologs MGL-1 and MGL-2 are key for sensing nutritional status in C. elegans
title_full_unstemmed The metabotropic glutamate receptor homologs MGL-1 and MGL-2 are key for sensing nutritional status in C. elegans
title_sort The metabotropic glutamate receptor homologs MGL-1 and MGL-2 are key for sensing nutritional status in C. elegans
dc.creator.none.fl_str_mv Lacour, Ailin
Blanco, Maria Gabriela
Zabala, Agustina
de Rosa, Maria Jose
Rayes, Diego Hernán
author Lacour, Ailin
author_facet Lacour, Ailin
Blanco, Maria Gabriela
Zabala, Agustina
de Rosa, Maria Jose
Rayes, Diego Hernán
author_role author
author2 Blanco, Maria Gabriela
Zabala, Agustina
de Rosa, Maria Jose
Rayes, Diego Hernán
author2_role author
author
author
author
dc.subject.none.fl_str_mv C. ELEGANS
SATIATION
NERVOUS CIRCUITS
FEEDING BEHAVIOR
topic C. ELEGANS
SATIATION
NERVOUS CIRCUITS
FEEDING BEHAVIOR
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The mechanisms that allow the nervous system to sense nutritional state and adapt animal behavior are poorly understood in most species. The simplicity of its NS and its known connectome make C. elegans a useful system to study these mechanisms. Results from our laboratory showed that inhibition of the tyraminergic neuron RIM during fasting, enhances serotonin release from other neurons when the animal reencounters food, allowing it to slow down locomotion and start feeding. Mutations in the GPCRs, MGL-1 and MGL-2, located in two presynaptic interneurons to RIM have been reported to induce autophagy even in well-fed animals. Here, we performed behavioral assays on mgl-1; mgl-2 mutants. We found that these animals, even when well fed, show a significant decrease in locomotion when they find food similar to fasted wild-type animals. Moreover, when we exposed these mutants to GFP-expressing bacteria, the fluorescence in the intestine is higher than that of wild-type animals, suggesting a higher feeding rate. These initial results suggest that the metabotropic receptors MGL-1 and MGL-2 are key for C. elegans to censor satiation molecules. We propose, therefore, to determine what these satiety signals are and the neuronal circuits involved. Given that this behavioral plasticity modulated by the nutritional state is observed throughout the animal kingdom, and that several fundamental processes are highly conserved, these results may provide universally relevant information
Fil: Lacour, Ailin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Blanco, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Zabala, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: de Rosa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Rayes, Diego Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Sociedad Argentina de Neurociencias Meeting (SAN2022)
Buenos Aires
Argentina
Sociedad Argentina de Neurociencias
description The mechanisms that allow the nervous system to sense nutritional state and adapt animal behavior are poorly understood in most species. The simplicity of its NS and its known connectome make C. elegans a useful system to study these mechanisms. Results from our laboratory showed that inhibition of the tyraminergic neuron RIM during fasting, enhances serotonin release from other neurons when the animal reencounters food, allowing it to slow down locomotion and start feeding. Mutations in the GPCRs, MGL-1 and MGL-2, located in two presynaptic interneurons to RIM have been reported to induce autophagy even in well-fed animals. Here, we performed behavioral assays on mgl-1; mgl-2 mutants. We found that these animals, even when well fed, show a significant decrease in locomotion when they find food similar to fasted wild-type animals. Moreover, when we exposed these mutants to GFP-expressing bacteria, the fluorescence in the intestine is higher than that of wild-type animals, suggesting a higher feeding rate. These initial results suggest that the metabotropic receptors MGL-1 and MGL-2 are key for C. elegans to censor satiation molecules. We propose, therefore, to determine what these satiety signals are and the neuronal circuits involved. Given that this behavioral plasticity modulated by the nutritional state is observed throughout the animal kingdom, and that several fundamental processes are highly conserved, these results may provide universally relevant information
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/229784
The metabotropic glutamate receptor homologs MGL-1 and MGL-2 are key for sensing nutritional status in C. elegans; Sociedad Argentina de Neurociencias Meeting (SAN2022); Buenos Aires; Argentina; 2022; 1-1
CONICET Digital
CONICET
url http://hdl.handle.net/11336/229784
identifier_str_mv The metabotropic glutamate receptor homologs MGL-1 and MGL-2 are key for sensing nutritional status in C. elegans; Sociedad Argentina de Neurociencias Meeting (SAN2022); Buenos Aires; Argentina; 2022; 1-1
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://csan2022.saneurociencias.org.ar/index.php/2022/09/23/214-effect-of-a-ketogenic-diet-on-the-expression-of-potassium-channels-controlling-neuronal-excitability/index.html
info:eu-repo/semantics/altIdentifier/url/https://csan2022.saneurociencias.org.ar/index.php/2022/09/23/182-the-metabotropic-glutamate-receptor-homologs-mgl-1-and-mgl-2-are-key-for-sensing-nutritional-status-in-c-elegans/index.html
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Neurociencias
publisher.none.fl_str_mv Sociedad Argentina de Neurociencias
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collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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