Clock gene homologs lin-42 and kin-20 regulate circadian rhythms in C. elegans
- Autores
- Lamberti, Melisa Luciana; Spangler, Rebecca K.; Cerdeira, Victoria; Ares, Myriam; Rivollet, Lise; Ashley, Guinevere E.; Coronado, Andrea Ramos; Tripathi, Sarvind; Spiousas, Ignacio; Ward, Jordan D.; Partch, Carrie L.; Bénard, Claire Y.; Goya, M. Eugenia; Golombek, Diego Andres
- Año de publicación
- 2024
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Circadian rhythms are endogenous oscillations in nearly all organisms, from prokaryotes to humans, allowing them to adapt to cyclical environments for close to 24 h. Circadian rhythms are regulatedby a central clock, based on a transcription-translation feedback loop. One important protein in the central loop in metazoan clocks is PERIOD, which is regulated in part by Casein kinase 1ε/δ (CK1ε/δ) phosphorylation. In the nematode Caenorhabditis elegans, period and casein kinase 1ε/δ are conserved as lin‐42 and kin‐20, respectively. Here, we studied the involvement of lin‐42 and kin‐20 in the circadian rhythms of the adult nematode using a bioluminescence-based circadian transcriptional reporter. We show that mutations of lin‐42 and kin‐20 generate a significantly longer endogenous period, suggesting a role for both genes in the nematode circadian clock, as in other organisms. These phenotypes can be partially rescued by overexpression of either gene under their native promoter. Both proteins are expressed in neurons and epidermal seam cells, as well as in other cells. Depletion of LIN-42 and KIN-20, specifically in neuronal cells after development, was sufficient to lengthenthe period of oscillating sur‐5 expression. Therefore, we conclude that LIN-42 and KIN-20 are critical regulators of the adult nematode circadian clock through neuronal cells.
Fil: Lamberti, Melisa Luciana. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Spangler, Rebecca K.. University Of California At Santa Cruz.; Estados Unidos
Fil: Cerdeira, Victoria. Université du Québec a Montreal; Canadá
Fil: Ares, Myriam. Université du Québec a Montreal; Canadá
Fil: Rivollet, Lise. Université du Québec a Montreal; Canadá
Fil: Ashley, Guinevere E.. University Of California At Santa Cruz.; Estados Unidos
Fil: Coronado, Andrea Ramos. University Of California At Santa Cruz.; Estados Unidos
Fil: Tripathi, Sarvind. University Of California At Santa Cruz.; Estados Unidos
Fil: Spiousas, Ignacio. Universidad de San Andrés; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ward, Jordan D.. University Of California At Santa Cruz.; Estados Unidos
Fil: Partch, Carrie L.. University Of California At Santa Cruz.; Estados Unidos
Fil: Bénard, Claire Y.. Université du Québec a Montreal; Canadá
Fil: Goya, M. Eugenia. University of Groningen; Países Bajos
Fil: Golombek, Diego Andres. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Materia
-
circadian
c elegans
lin42
kin20 - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/238761
Ver los metadatos del registro completo
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Clock gene homologs lin-42 and kin-20 regulate circadian rhythms in C. elegansLamberti, Melisa LucianaSpangler, Rebecca K.Cerdeira, VictoriaAres, MyriamRivollet, LiseAshley, Guinevere E.Coronado, Andrea RamosTripathi, SarvindSpiousas, IgnacioWard, Jordan D.Partch, Carrie L.Bénard, Claire Y.Goya, M. EugeniaGolombek, Diego Andrescircadianc eleganslin42kin20https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Circadian rhythms are endogenous oscillations in nearly all organisms, from prokaryotes to humans, allowing them to adapt to cyclical environments for close to 24 h. Circadian rhythms are regulatedby a central clock, based on a transcription-translation feedback loop. One important protein in the central loop in metazoan clocks is PERIOD, which is regulated in part by Casein kinase 1ε/δ (CK1ε/δ) phosphorylation. In the nematode Caenorhabditis elegans, period and casein kinase 1ε/δ are conserved as lin‐42 and kin‐20, respectively. Here, we studied the involvement of lin‐42 and kin‐20 in the circadian rhythms of the adult nematode using a bioluminescence-based circadian transcriptional reporter. We show that mutations of lin‐42 and kin‐20 generate a significantly longer endogenous period, suggesting a role for both genes in the nematode circadian clock, as in other organisms. These phenotypes can be partially rescued by overexpression of either gene under their native promoter. Both proteins are expressed in neurons and epidermal seam cells, as well as in other cells. Depletion of LIN-42 and KIN-20, specifically in neuronal cells after development, was sufficient to lengthenthe period of oscillating sur‐5 expression. Therefore, we conclude that LIN-42 and KIN-20 are critical regulators of the adult nematode circadian clock through neuronal cells.Fil: Lamberti, Melisa Luciana. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Spangler, Rebecca K.. University Of California At Santa Cruz.; Estados UnidosFil: Cerdeira, Victoria. Université du Québec a Montreal; CanadáFil: Ares, Myriam. Université du Québec a Montreal; CanadáFil: Rivollet, Lise. Université du Québec a Montreal; CanadáFil: Ashley, Guinevere E.. University Of California At Santa Cruz.; Estados UnidosFil: Coronado, Andrea Ramos. University Of California At Santa Cruz.; Estados UnidosFil: Tripathi, Sarvind. University Of California At Santa Cruz.; Estados UnidosFil: Spiousas, Ignacio. Universidad de San Andrés; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ward, Jordan D.. University Of California At Santa Cruz.; Estados UnidosFil: Partch, Carrie L.. University Of California At Santa Cruz.; Estados UnidosFil: Bénard, Claire Y.. Université du Québec a Montreal; CanadáFil: Goya, M. Eugenia. University of Groningen; Países BajosFil: Golombek, Diego Andres. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaNature2024-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/238761Lamberti, Melisa Luciana; Spangler, Rebecca K.; Cerdeira, Victoria; Ares, Myriam; Rivollet, Lise; et al.; Clock gene homologs lin-42 and kin-20 regulate circadian rhythms in C. elegans; Nature; Scientific Reports; 14; 1; 6-2024; 1-172045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-024-62303-9info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-024-62303-9info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:53:44Zoai:ri.conicet.gov.ar:11336/238761instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:53:44.477CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Clock gene homologs lin-42 and kin-20 regulate circadian rhythms in C. elegans |
| title |
Clock gene homologs lin-42 and kin-20 regulate circadian rhythms in C. elegans |
| spellingShingle |
Clock gene homologs lin-42 and kin-20 regulate circadian rhythms in C. elegans Lamberti, Melisa Luciana circadian c elegans lin42 kin20 |
| title_short |
Clock gene homologs lin-42 and kin-20 regulate circadian rhythms in C. elegans |
| title_full |
Clock gene homologs lin-42 and kin-20 regulate circadian rhythms in C. elegans |
| title_fullStr |
Clock gene homologs lin-42 and kin-20 regulate circadian rhythms in C. elegans |
| title_full_unstemmed |
Clock gene homologs lin-42 and kin-20 regulate circadian rhythms in C. elegans |
| title_sort |
Clock gene homologs lin-42 and kin-20 regulate circadian rhythms in C. elegans |
| dc.creator.none.fl_str_mv |
Lamberti, Melisa Luciana Spangler, Rebecca K. Cerdeira, Victoria Ares, Myriam Rivollet, Lise Ashley, Guinevere E. Coronado, Andrea Ramos Tripathi, Sarvind Spiousas, Ignacio Ward, Jordan D. Partch, Carrie L. Bénard, Claire Y. Goya, M. Eugenia Golombek, Diego Andres |
| author |
Lamberti, Melisa Luciana |
| author_facet |
Lamberti, Melisa Luciana Spangler, Rebecca K. Cerdeira, Victoria Ares, Myriam Rivollet, Lise Ashley, Guinevere E. Coronado, Andrea Ramos Tripathi, Sarvind Spiousas, Ignacio Ward, Jordan D. Partch, Carrie L. Bénard, Claire Y. Goya, M. Eugenia Golombek, Diego Andres |
| author_role |
author |
| author2 |
Spangler, Rebecca K. Cerdeira, Victoria Ares, Myriam Rivollet, Lise Ashley, Guinevere E. Coronado, Andrea Ramos Tripathi, Sarvind Spiousas, Ignacio Ward, Jordan D. Partch, Carrie L. Bénard, Claire Y. Goya, M. Eugenia Golombek, Diego Andres |
| author2_role |
author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
circadian c elegans lin42 kin20 |
| topic |
circadian c elegans lin42 kin20 |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
Circadian rhythms are endogenous oscillations in nearly all organisms, from prokaryotes to humans, allowing them to adapt to cyclical environments for close to 24 h. Circadian rhythms are regulatedby a central clock, based on a transcription-translation feedback loop. One important protein in the central loop in metazoan clocks is PERIOD, which is regulated in part by Casein kinase 1ε/δ (CK1ε/δ) phosphorylation. In the nematode Caenorhabditis elegans, period and casein kinase 1ε/δ are conserved as lin‐42 and kin‐20, respectively. Here, we studied the involvement of lin‐42 and kin‐20 in the circadian rhythms of the adult nematode using a bioluminescence-based circadian transcriptional reporter. We show that mutations of lin‐42 and kin‐20 generate a significantly longer endogenous period, suggesting a role for both genes in the nematode circadian clock, as in other organisms. These phenotypes can be partially rescued by overexpression of either gene under their native promoter. Both proteins are expressed in neurons and epidermal seam cells, as well as in other cells. Depletion of LIN-42 and KIN-20, specifically in neuronal cells after development, was sufficient to lengthenthe period of oscillating sur‐5 expression. Therefore, we conclude that LIN-42 and KIN-20 are critical regulators of the adult nematode circadian clock through neuronal cells. Fil: Lamberti, Melisa Luciana. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Spangler, Rebecca K.. University Of California At Santa Cruz.; Estados Unidos Fil: Cerdeira, Victoria. Université du Québec a Montreal; Canadá Fil: Ares, Myriam. Université du Québec a Montreal; Canadá Fil: Rivollet, Lise. Université du Québec a Montreal; Canadá Fil: Ashley, Guinevere E.. University Of California At Santa Cruz.; Estados Unidos Fil: Coronado, Andrea Ramos. University Of California At Santa Cruz.; Estados Unidos Fil: Tripathi, Sarvind. University Of California At Santa Cruz.; Estados Unidos Fil: Spiousas, Ignacio. Universidad de San Andrés; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ward, Jordan D.. University Of California At Santa Cruz.; Estados Unidos Fil: Partch, Carrie L.. University Of California At Santa Cruz.; Estados Unidos Fil: Bénard, Claire Y.. Université du Québec a Montreal; Canadá Fil: Goya, M. Eugenia. University of Groningen; Países Bajos Fil: Golombek, Diego Andres. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
| description |
Circadian rhythms are endogenous oscillations in nearly all organisms, from prokaryotes to humans, allowing them to adapt to cyclical environments for close to 24 h. Circadian rhythms are regulatedby a central clock, based on a transcription-translation feedback loop. One important protein in the central loop in metazoan clocks is PERIOD, which is regulated in part by Casein kinase 1ε/δ (CK1ε/δ) phosphorylation. In the nematode Caenorhabditis elegans, period and casein kinase 1ε/δ are conserved as lin‐42 and kin‐20, respectively. Here, we studied the involvement of lin‐42 and kin‐20 in the circadian rhythms of the adult nematode using a bioluminescence-based circadian transcriptional reporter. We show that mutations of lin‐42 and kin‐20 generate a significantly longer endogenous period, suggesting a role for both genes in the nematode circadian clock, as in other organisms. These phenotypes can be partially rescued by overexpression of either gene under their native promoter. Both proteins are expressed in neurons and epidermal seam cells, as well as in other cells. Depletion of LIN-42 and KIN-20, specifically in neuronal cells after development, was sufficient to lengthenthe period of oscillating sur‐5 expression. Therefore, we conclude that LIN-42 and KIN-20 are critical regulators of the adult nematode circadian clock through neuronal cells. |
| publishDate |
2024 |
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2024-06 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
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http://hdl.handle.net/11336/238761 Lamberti, Melisa Luciana; Spangler, Rebecca K.; Cerdeira, Victoria; Ares, Myriam; Rivollet, Lise; et al.; Clock gene homologs lin-42 and kin-20 regulate circadian rhythms in C. elegans; Nature; Scientific Reports; 14; 1; 6-2024; 1-17 2045-2322 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/238761 |
| identifier_str_mv |
Lamberti, Melisa Luciana; Spangler, Rebecca K.; Cerdeira, Victoria; Ares, Myriam; Rivollet, Lise; et al.; Clock gene homologs lin-42 and kin-20 regulate circadian rhythms in C. elegans; Nature; Scientific Reports; 14; 1; 6-2024; 1-17 2045-2322 CONICET Digital CONICET |
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eng |
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eng |
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