A gene regulatory network controlled by the NAC transcription factor ANAC092/AtNAC2/ORE1 during salt-promoted senescence

Autores
Balazadeh, Salma; Siddiqui, Hamad; Allu, Annapurna D.; Matallana Ramirez, Lilian P.; Caldana, Camila; Mehrnia, Mohammad; Zanor, María Inés; Köhler, Barbara; Mueller Roeber, Bernd
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Onset and progression of senescence are under genetic and environmental control. The Arabidopsis thaliana NAC transcription factor ANAC092 (also called AtNAC2 and ORE1) has recently been shown to control age-dependent senescence, but its mode of action has not been analyzed yet. To explore the regulatory network administered by ANAC092 we performed microarray-based expression profiling using estradiol-inducible ANAC092 overexpression lines. Approximately 39% of the 170 genes up-regulated upon ANAC092 induction are known senescence-associated genes, suggesting the NAC factor exerts its role in senescence through a regulatory network that includes many of the genes previously reported to be senescence regulated. We selected 39 candidate genes and confirmed their time-dependent response to enhanced ANAC092 expression by quantitative RT-PCR. We also found that the majority of them (24 genes) is up-regulated by salt stress, a major promoter of plant senescence, in a manner similar to that of ANAC092 which itself is salt-responsive. Furthermore, 24 genes like ANAC092 turned out to be stage-dependently expressed during seed growth with marginal expression at early and elevated expression at late stages of seed development. Disruption of ANAC092 increased the rate of seed germination under saline conditions, whereas the opposite occurred in respective overexpression plants. We also detected a delay of salinity induced chlorophyll loss in detached anac092-1 mutant leaves. Promoter-reporter (GUS) studies revealed transcriptional control of ANAC092 expression during leaf and flower aging and in response to salt stress. We conclude that ANAC092 exerts its functions during senescence and seed germination through partially overlapping gene sets.
Fil: Balazadeh, Salma. Universitat Potsdam; Alemania
Fil: Siddiqui, Hamad. Universitat Potsdam; Alemania
Fil: Allu, Annapurna D.. Universitat Potsdam; Alemania
Fil: Matallana Ramirez, Lilian P.. Universitat Potsdam; Alemania
Fil: Caldana, Camila. Universitat Potsdam; Alemania
Fil: Mehrnia, Mohammad. Universitat Potsdam; Alemania
Fil: Zanor, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Köhler, Barbara. Universitat Potsdam; Alemania
Fil: Mueller Roeber, Bernd. Universitat Potsdam; Alemania
Materia
GENE EXPRESSION
GERMINATION
LEAF SENESCENCE
SALT TOLERANCE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/277326
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A gene regulatory network controlled by the NAC transcription factor ANAC092/AtNAC2/ORE1 during salt-promoted senescenceBalazadeh, SalmaSiddiqui, HamadAllu, Annapurna D.Matallana Ramirez, Lilian P.Caldana, CamilaMehrnia, MohammadZanor, María InésKöhler, BarbaraMueller Roeber, BerndGENE EXPRESSIONGERMINATIONLEAF SENESCENCESALT TOLERANCEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Onset and progression of senescence are under genetic and environmental control. The Arabidopsis thaliana NAC transcription factor ANAC092 (also called AtNAC2 and ORE1) has recently been shown to control age-dependent senescence, but its mode of action has not been analyzed yet. To explore the regulatory network administered by ANAC092 we performed microarray-based expression profiling using estradiol-inducible ANAC092 overexpression lines. Approximately 39% of the 170 genes up-regulated upon ANAC092 induction are known senescence-associated genes, suggesting the NAC factor exerts its role in senescence through a regulatory network that includes many of the genes previously reported to be senescence regulated. We selected 39 candidate genes and confirmed their time-dependent response to enhanced ANAC092 expression by quantitative RT-PCR. We also found that the majority of them (24 genes) is up-regulated by salt stress, a major promoter of plant senescence, in a manner similar to that of ANAC092 which itself is salt-responsive. Furthermore, 24 genes like ANAC092 turned out to be stage-dependently expressed during seed growth with marginal expression at early and elevated expression at late stages of seed development. Disruption of ANAC092 increased the rate of seed germination under saline conditions, whereas the opposite occurred in respective overexpression plants. We also detected a delay of salinity induced chlorophyll loss in detached anac092-1 mutant leaves. Promoter-reporter (GUS) studies revealed transcriptional control of ANAC092 expression during leaf and flower aging and in response to salt stress. We conclude that ANAC092 exerts its functions during senescence and seed germination through partially overlapping gene sets.Fil: Balazadeh, Salma. Universitat Potsdam; AlemaniaFil: Siddiqui, Hamad. Universitat Potsdam; AlemaniaFil: Allu, Annapurna D.. Universitat Potsdam; AlemaniaFil: Matallana Ramirez, Lilian P.. Universitat Potsdam; AlemaniaFil: Caldana, Camila. Universitat Potsdam; AlemaniaFil: Mehrnia, Mohammad. Universitat Potsdam; AlemaniaFil: Zanor, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Köhler, Barbara. Universitat Potsdam; AlemaniaFil: Mueller Roeber, Bernd. Universitat Potsdam; AlemaniaWiley Blackwell Publishing, Inc2010-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/277326Balazadeh, Salma; Siddiqui, Hamad; Allu, Annapurna D.; Matallana Ramirez, Lilian P.; Caldana, Camila; et al.; A gene regulatory network controlled by the NAC transcription factor ANAC092/AtNAC2/ORE1 during salt-promoted senescence; Wiley Blackwell Publishing, Inc; Plant Journal; 62; 2; 3-2010; 250-2640960-7412CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/j.1365-313X.2010.04151.xinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-313X.2010.04151.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-23T13:52:07Zoai:ri.conicet.gov.ar:11336/277326instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-23 13:52:08.19CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A gene regulatory network controlled by the NAC transcription factor ANAC092/AtNAC2/ORE1 during salt-promoted senescence
title A gene regulatory network controlled by the NAC transcription factor ANAC092/AtNAC2/ORE1 during salt-promoted senescence
spellingShingle A gene regulatory network controlled by the NAC transcription factor ANAC092/AtNAC2/ORE1 during salt-promoted senescence
Balazadeh, Salma
GENE EXPRESSION
GERMINATION
LEAF SENESCENCE
SALT TOLERANCE
title_short A gene regulatory network controlled by the NAC transcription factor ANAC092/AtNAC2/ORE1 during salt-promoted senescence
title_full A gene regulatory network controlled by the NAC transcription factor ANAC092/AtNAC2/ORE1 during salt-promoted senescence
title_fullStr A gene regulatory network controlled by the NAC transcription factor ANAC092/AtNAC2/ORE1 during salt-promoted senescence
title_full_unstemmed A gene regulatory network controlled by the NAC transcription factor ANAC092/AtNAC2/ORE1 during salt-promoted senescence
title_sort A gene regulatory network controlled by the NAC transcription factor ANAC092/AtNAC2/ORE1 during salt-promoted senescence
dc.creator.none.fl_str_mv Balazadeh, Salma
Siddiqui, Hamad
Allu, Annapurna D.
Matallana Ramirez, Lilian P.
Caldana, Camila
Mehrnia, Mohammad
Zanor, María Inés
Köhler, Barbara
Mueller Roeber, Bernd
author Balazadeh, Salma
author_facet Balazadeh, Salma
Siddiqui, Hamad
Allu, Annapurna D.
Matallana Ramirez, Lilian P.
Caldana, Camila
Mehrnia, Mohammad
Zanor, María Inés
Köhler, Barbara
Mueller Roeber, Bernd
author_role author
author2 Siddiqui, Hamad
Allu, Annapurna D.
Matallana Ramirez, Lilian P.
Caldana, Camila
Mehrnia, Mohammad
Zanor, María Inés
Köhler, Barbara
Mueller Roeber, Bernd
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv GENE EXPRESSION
GERMINATION
LEAF SENESCENCE
SALT TOLERANCE
topic GENE EXPRESSION
GERMINATION
LEAF SENESCENCE
SALT TOLERANCE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Onset and progression of senescence are under genetic and environmental control. The Arabidopsis thaliana NAC transcription factor ANAC092 (also called AtNAC2 and ORE1) has recently been shown to control age-dependent senescence, but its mode of action has not been analyzed yet. To explore the regulatory network administered by ANAC092 we performed microarray-based expression profiling using estradiol-inducible ANAC092 overexpression lines. Approximately 39% of the 170 genes up-regulated upon ANAC092 induction are known senescence-associated genes, suggesting the NAC factor exerts its role in senescence through a regulatory network that includes many of the genes previously reported to be senescence regulated. We selected 39 candidate genes and confirmed their time-dependent response to enhanced ANAC092 expression by quantitative RT-PCR. We also found that the majority of them (24 genes) is up-regulated by salt stress, a major promoter of plant senescence, in a manner similar to that of ANAC092 which itself is salt-responsive. Furthermore, 24 genes like ANAC092 turned out to be stage-dependently expressed during seed growth with marginal expression at early and elevated expression at late stages of seed development. Disruption of ANAC092 increased the rate of seed germination under saline conditions, whereas the opposite occurred in respective overexpression plants. We also detected a delay of salinity induced chlorophyll loss in detached anac092-1 mutant leaves. Promoter-reporter (GUS) studies revealed transcriptional control of ANAC092 expression during leaf and flower aging and in response to salt stress. We conclude that ANAC092 exerts its functions during senescence and seed germination through partially overlapping gene sets.
Fil: Balazadeh, Salma. Universitat Potsdam; Alemania
Fil: Siddiqui, Hamad. Universitat Potsdam; Alemania
Fil: Allu, Annapurna D.. Universitat Potsdam; Alemania
Fil: Matallana Ramirez, Lilian P.. Universitat Potsdam; Alemania
Fil: Caldana, Camila. Universitat Potsdam; Alemania
Fil: Mehrnia, Mohammad. Universitat Potsdam; Alemania
Fil: Zanor, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Köhler, Barbara. Universitat Potsdam; Alemania
Fil: Mueller Roeber, Bernd. Universitat Potsdam; Alemania
description Onset and progression of senescence are under genetic and environmental control. The Arabidopsis thaliana NAC transcription factor ANAC092 (also called AtNAC2 and ORE1) has recently been shown to control age-dependent senescence, but its mode of action has not been analyzed yet. To explore the regulatory network administered by ANAC092 we performed microarray-based expression profiling using estradiol-inducible ANAC092 overexpression lines. Approximately 39% of the 170 genes up-regulated upon ANAC092 induction are known senescence-associated genes, suggesting the NAC factor exerts its role in senescence through a regulatory network that includes many of the genes previously reported to be senescence regulated. We selected 39 candidate genes and confirmed their time-dependent response to enhanced ANAC092 expression by quantitative RT-PCR. We also found that the majority of them (24 genes) is up-regulated by salt stress, a major promoter of plant senescence, in a manner similar to that of ANAC092 which itself is salt-responsive. Furthermore, 24 genes like ANAC092 turned out to be stage-dependently expressed during seed growth with marginal expression at early and elevated expression at late stages of seed development. Disruption of ANAC092 increased the rate of seed germination under saline conditions, whereas the opposite occurred in respective overexpression plants. We also detected a delay of salinity induced chlorophyll loss in detached anac092-1 mutant leaves. Promoter-reporter (GUS) studies revealed transcriptional control of ANAC092 expression during leaf and flower aging and in response to salt stress. We conclude that ANAC092 exerts its functions during senescence and seed germination through partially overlapping gene sets.
publishDate 2010
dc.date.none.fl_str_mv 2010-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/277326
Balazadeh, Salma; Siddiqui, Hamad; Allu, Annapurna D.; Matallana Ramirez, Lilian P.; Caldana, Camila; et al.; A gene regulatory network controlled by the NAC transcription factor ANAC092/AtNAC2/ORE1 during salt-promoted senescence; Wiley Blackwell Publishing, Inc; Plant Journal; 62; 2; 3-2010; 250-264
0960-7412
CONICET Digital
CONICET
url http://hdl.handle.net/11336/277326
identifier_str_mv Balazadeh, Salma; Siddiqui, Hamad; Allu, Annapurna D.; Matallana Ramirez, Lilian P.; Caldana, Camila; et al.; A gene regulatory network controlled by the NAC transcription factor ANAC092/AtNAC2/ORE1 during salt-promoted senescence; Wiley Blackwell Publishing, Inc; Plant Journal; 62; 2; 3-2010; 250-264
0960-7412
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/j.1365-313X.2010.04151.x
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-313X.2010.04151.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.952241

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