Identification and expression analysis of NAC transcription factors potentially involved in leaf and petal senescence in Petunia hybrida

Autores
Trupkin, Santiago Ariel; Astigueta, Francisco H.; Baigorria, Amilcar Hernan; Garcia, Martin Nahuel; Delfosse, Veronica Cecilia; González, Sergio A.; Perez De La Torre, Mariana; Moschen, Sebastian; Lia, Veronica Viviana; Fernandez, Paula Del Carmen; Heinz, Ruth Amelia
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Progression of leaf senescence depends on several families of transcription factors. In Arabidopsis, the NAC family plays crucial roles in the modulation of leaf senescence; however, the mechanisms involved in this NAC-mediated regulation have not been extensively explored in agronomic species. Petunia hybrida is an ornamental plant that is commonly found worldwide. Decreasing the rate of leaf and petal senescence in P. hybrida is essential for maintaining plant quality. In this study, we examined the NAC-mediated networks involved in regulating senescence in this species. From 41 NAC genes, the expression of which changed in Arabidopsis during leaf senescence, we identified 29 putative orthologs in P. hybrida. Analysis using quantitative real-time-PCR indicated that 24 genes in P. hybrida changed their transcript levels during natural leaf senescence. Leaf-expressed genes were subsequently assessed in petals undergoing natural and pollination-induced senescence. Expression data and phylogenetic analysis were used to generate a list of 10–15 candidate genes; 7 of these were considered key regulatory candidates in senescence because of their consistent upregulation in the three senescence processes examined. Altogether, we identified common and distinct patterns of gene expression at different stages of leaf and petal development and during progression of senescence. The results obtained in this study will contribute to the understanding of NAC-mediated regulatory networks in petunia.
Instituto de Floricultura
Fil: Trupkin, Santiago Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Astigueta, Francisco H. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina
Fil: Baigorria, Amilcar Hernan. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina
Fil: Garcia, Martin Nahuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Delfosse, Verónica C. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina
Fil: González, Sergio A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Perez De La Torre, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentina
Fil: Moschen, Sebastian Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lia, Veronica Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Fernandez, Paula Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina
Fil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fuente
Plant Science 287 : 110195 (October 2019)
Materia
Petunia hybrida
Fitomejoramiento
Genética
Factores de Transcripción
Expresión Génica
Avejentamiento
Plant Breeding
Genetics
Transcription Factors
Gene Expression
Senescence
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/5683
Acceder
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oai_identifier_str oai:localhost:20.500.12123/5683
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Identification and expression analysis of NAC transcription factors potentially involved in leaf and petal senescence in Petunia hybridaTrupkin, Santiago ArielAstigueta, Francisco H.Baigorria, Amilcar HernanGarcia, Martin NahuelDelfosse, Veronica CeciliaGonzález, Sergio A.Perez De La Torre, MarianaMoschen, SebastianLia, Veronica VivianaFernandez, Paula Del CarmenHeinz, Ruth AmeliaPetunia hybridaFitomejoramientoGenéticaFactores de TranscripciónExpresión GénicaAvejentamientoPlant BreedingGeneticsTranscription FactorsGene ExpressionSenescenceProgression of leaf senescence depends on several families of transcription factors. In Arabidopsis, the NAC family plays crucial roles in the modulation of leaf senescence; however, the mechanisms involved in this NAC-mediated regulation have not been extensively explored in agronomic species. Petunia hybrida is an ornamental plant that is commonly found worldwide. Decreasing the rate of leaf and petal senescence in P. hybrida is essential for maintaining plant quality. In this study, we examined the NAC-mediated networks involved in regulating senescence in this species. From 41 NAC genes, the expression of which changed in Arabidopsis during leaf senescence, we identified 29 putative orthologs in P. hybrida. Analysis using quantitative real-time-PCR indicated that 24 genes in P. hybrida changed their transcript levels during natural leaf senescence. Leaf-expressed genes were subsequently assessed in petals undergoing natural and pollination-induced senescence. Expression data and phylogenetic analysis were used to generate a list of 10–15 candidate genes; 7 of these were considered key regulatory candidates in senescence because of their consistent upregulation in the three senescence processes examined. Altogether, we identified common and distinct patterns of gene expression at different stages of leaf and petal development and during progression of senescence. The results obtained in this study will contribute to the understanding of NAC-mediated regulatory networks in petunia.Instituto de FloriculturaFil: Trupkin, Santiago Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Astigueta, Francisco H. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; ArgentinaFil: Baigorria, Amilcar Hernan. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; ArgentinaFil: Garcia, Martin Nahuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Delfosse, Verónica C. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; ArgentinaFil: González, Sergio A. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Perez De La Torre, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; ArgentinaFil: Moschen, Sebastian Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lia, Veronica Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Fernandez, Paula Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; ArgentinaFil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaElsevier2019-08-26T11:30:13Z2019-08-26T11:30:13Z2019-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.sciencedirect.com/science/article/pii/S0168945219305862http://hdl.handle.net/20.500.12123/56830168-9452https://doi.org/10.1016/j.plantsci.2019.110195Plant Science 287 : 110195 (October 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:48:08Zoai:localhost:20.500.12123/5683instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:48:09.138INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Identification and expression analysis of NAC transcription factors potentially involved in leaf and petal senescence in Petunia hybrida
title Identification and expression analysis of NAC transcription factors potentially involved in leaf and petal senescence in Petunia hybrida
spellingShingle Identification and expression analysis of NAC transcription factors potentially involved in leaf and petal senescence in Petunia hybrida
Trupkin, Santiago Ariel
Petunia hybrida
Fitomejoramiento
Genética
Factores de Transcripción
Expresión Génica
Avejentamiento
Plant Breeding
Genetics
Transcription Factors
Gene Expression
Senescence
title_short Identification and expression analysis of NAC transcription factors potentially involved in leaf and petal senescence in Petunia hybrida
title_full Identification and expression analysis of NAC transcription factors potentially involved in leaf and petal senescence in Petunia hybrida
title_fullStr Identification and expression analysis of NAC transcription factors potentially involved in leaf and petal senescence in Petunia hybrida
title_full_unstemmed Identification and expression analysis of NAC transcription factors potentially involved in leaf and petal senescence in Petunia hybrida
title_sort Identification and expression analysis of NAC transcription factors potentially involved in leaf and petal senescence in Petunia hybrida
dc.creator.none.fl_str_mv Trupkin, Santiago Ariel
Astigueta, Francisco H.
Baigorria, Amilcar Hernan
Garcia, Martin Nahuel
Delfosse, Veronica Cecilia
González, Sergio A.
Perez De La Torre, Mariana
Moschen, Sebastian
Lia, Veronica Viviana
Fernandez, Paula Del Carmen
Heinz, Ruth Amelia
author Trupkin, Santiago Ariel
author_facet Trupkin, Santiago Ariel
Astigueta, Francisco H.
Baigorria, Amilcar Hernan
Garcia, Martin Nahuel
Delfosse, Veronica Cecilia
González, Sergio A.
Perez De La Torre, Mariana
Moschen, Sebastian
Lia, Veronica Viviana
Fernandez, Paula Del Carmen
Heinz, Ruth Amelia
author_role author
author2 Astigueta, Francisco H.
Baigorria, Amilcar Hernan
Garcia, Martin Nahuel
Delfosse, Veronica Cecilia
González, Sergio A.
Perez De La Torre, Mariana
Moschen, Sebastian
Lia, Veronica Viviana
Fernandez, Paula Del Carmen
Heinz, Ruth Amelia
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Petunia hybrida
Fitomejoramiento
Genética
Factores de Transcripción
Expresión Génica
Avejentamiento
Plant Breeding
Genetics
Transcription Factors
Gene Expression
Senescence
topic Petunia hybrida
Fitomejoramiento
Genética
Factores de Transcripción
Expresión Génica
Avejentamiento
Plant Breeding
Genetics
Transcription Factors
Gene Expression
Senescence
dc.description.none.fl_txt_mv Progression of leaf senescence depends on several families of transcription factors. In Arabidopsis, the NAC family plays crucial roles in the modulation of leaf senescence; however, the mechanisms involved in this NAC-mediated regulation have not been extensively explored in agronomic species. Petunia hybrida is an ornamental plant that is commonly found worldwide. Decreasing the rate of leaf and petal senescence in P. hybrida is essential for maintaining plant quality. In this study, we examined the NAC-mediated networks involved in regulating senescence in this species. From 41 NAC genes, the expression of which changed in Arabidopsis during leaf senescence, we identified 29 putative orthologs in P. hybrida. Analysis using quantitative real-time-PCR indicated that 24 genes in P. hybrida changed their transcript levels during natural leaf senescence. Leaf-expressed genes were subsequently assessed in petals undergoing natural and pollination-induced senescence. Expression data and phylogenetic analysis were used to generate a list of 10–15 candidate genes; 7 of these were considered key regulatory candidates in senescence because of their consistent upregulation in the three senescence processes examined. Altogether, we identified common and distinct patterns of gene expression at different stages of leaf and petal development and during progression of senescence. The results obtained in this study will contribute to the understanding of NAC-mediated regulatory networks in petunia.
Instituto de Floricultura
Fil: Trupkin, Santiago Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Astigueta, Francisco H. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina
Fil: Baigorria, Amilcar Hernan. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina
Fil: Garcia, Martin Nahuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Delfosse, Verónica C. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina
Fil: González, Sergio A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Perez De La Torre, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentina
Fil: Moschen, Sebastian Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lia, Veronica Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Fernandez, Paula Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina
Fil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
description Progression of leaf senescence depends on several families of transcription factors. In Arabidopsis, the NAC family plays crucial roles in the modulation of leaf senescence; however, the mechanisms involved in this NAC-mediated regulation have not been extensively explored in agronomic species. Petunia hybrida is an ornamental plant that is commonly found worldwide. Decreasing the rate of leaf and petal senescence in P. hybrida is essential for maintaining plant quality. In this study, we examined the NAC-mediated networks involved in regulating senescence in this species. From 41 NAC genes, the expression of which changed in Arabidopsis during leaf senescence, we identified 29 putative orthologs in P. hybrida. Analysis using quantitative real-time-PCR indicated that 24 genes in P. hybrida changed their transcript levels during natural leaf senescence. Leaf-expressed genes were subsequently assessed in petals undergoing natural and pollination-induced senescence. Expression data and phylogenetic analysis were used to generate a list of 10–15 candidate genes; 7 of these were considered key regulatory candidates in senescence because of their consistent upregulation in the three senescence processes examined. Altogether, we identified common and distinct patterns of gene expression at different stages of leaf and petal development and during progression of senescence. The results obtained in this study will contribute to the understanding of NAC-mediated regulatory networks in petunia.
publishDate 2019
dc.date.none.fl_str_mv 2019-08-26T11:30:13Z
2019-08-26T11:30:13Z
2019-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0168945219305862
http://hdl.handle.net/20.500.12123/5683
0168-9452
https://doi.org/10.1016/j.plantsci.2019.110195
url https://www.sciencedirect.com/science/article/pii/S0168945219305862
http://hdl.handle.net/20.500.12123/5683
https://doi.org/10.1016/j.plantsci.2019.110195
identifier_str_mv 0168-9452
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Plant Science 287 : 110195 (October 2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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