JUNGBRUNNEN1, a Reactive Oxygen Species–Responsive NAC Transcription Factor, Regulates Longevity in Arabidopsis
- Autores
- Wu, Anhui; Allu, Annapurna Devi; Garapati, Prashanth; Siddiqui, Hamad; Dortay, Hakan; Zanor, María Inés; Asensi Fabado, Maria Amparo; Munné Bosch, Sergi; Antonio, Carla; Tohge, Takayuki; Fernie, Alisdair R.; Kaufmann, Kerstin; Xue, Gang-Ping; Mueller Roeber, Bernd; Balazadeh, Salma
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The transition from juvenility over maturation to senescence is a complex process involving multiple transcription factors and regulatory circuits, most of which are currently only vaguely defined. Here we identify JUNGBRUNNEN1 (JUB1), a NAC transcription factor gene rapidly induced by hydrogen peroxide (H2O2), as a central regulator of juvenility in Arabidopsis thaliana. Overexpression of JUB1 under constitutive and inducible promoters strongly delays senescence, enhances tolerance to various abiotic stresses including oxidative stress, and dampens the intracellular H2O2 level. In contrast, precocious senescence and lowered tolerance to abiotic stress were evident in the jub1-1 knock-down line. In vitro binding site selection identified the preferred JUB1 binding site containing RRYGCCGT as its core sequence. The JUB1 binding site is present within the 5´ upstream regulatory region of DREB2A, a transcription factor playing an important role in abiotic stress responses. In accordance with this, JUB1 transactivates DREB2A expression in mesophyll cell protoplasts and transgenic plants and binds in vitro an in vivo to the DREB2A promoter. Transcriptome profiling of JUB1 overexpressors revealed elevated expression of several reactive oxygen species (ROS) responsive genes, including various heat shock protein (HSP) and ROS scavenging glutathione S-transferase (GST) genes, whose expression is further induced by H2O2 treatment. Metabolite profiling revealed that levels of proline and trehalose were significantly elevated in JUB1 overexpression plants, in accordance with their enhanced abiotic stress tolerance. We hypothesize that JUB1 constitutes a central regulator of a finely tuned control system that modulates cellular H2O2 level and primes the plants for upcoming stress through a gene regulatory network that involves DREB2A.
Fil: Wu, Anhui. Universitat Potsdam; Alemania. Institut Max Planck fur Molekulare Physiologie; Alemania
Fil: Allu, Annapurna Devi. Universitat Potsdam; Alemania. Institut Max Planck fur Molekulare Physiologie; Alemania
Fil: Garapati, Prashanth. Universitat Potsdam; Alemania. Institut Max Planck fur Molekulare Physiologie; Alemania
Fil: Siddiqui, Hamad. Universitat Potsdam; Alemania
Fil: Dortay, Hakan. Universitat Potsdam; Alemania
Fil: Zanor, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Institut Max Planck fur Molekulare Physiologie; Alemania
Fil: Asensi Fabado, Maria Amparo. Universidad de Barcelona; España
Fil: Munné Bosch, Sergi. Universidad de Barcelona; España
Fil: Antonio, Carla. Institut Max Planck fur Molekulare Physiologie; Alemania
Fil: Tohge, Takayuki. Institut Max Planck fur Molekulare Physiologie; Alemania
Fil: Fernie, Alisdair R.. Institut Max Planck fur Molekulare Physiologie; Alemania
Fil: Kaufmann, Kerstin. University of Agriculture Wageningen; Países Bajos
Fil: Xue, Gang-Ping. Commonwealth Scientific And Industrial Research Organisation (csiro);
Fil: Mueller Roeber, Bernd. Universitat Potsdam; Alemania. Institut Max Planck fur Molekulare Physiologie; Alemania
Fil: Balazadeh, Salma. Universitat Potsdam; Alemania. Institut Max Planck fur Molekulare Physiologie; Alemania - Materia
-
ARABIDOPSIS
SENESCENCE
HYDROGEN PEROXIDE - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/268994
Ver los metadatos del registro completo
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JUNGBRUNNEN1, a Reactive Oxygen Species–Responsive NAC Transcription Factor, Regulates Longevity in ArabidopsisWu, AnhuiAllu, Annapurna DeviGarapati, PrashanthSiddiqui, HamadDortay, HakanZanor, María InésAsensi Fabado, Maria AmparoMunné Bosch, SergiAntonio, CarlaTohge, TakayukiFernie, Alisdair R.Kaufmann, KerstinXue, Gang-PingMueller Roeber, BerndBalazadeh, SalmaARABIDOPSISSENESCENCEHYDROGEN PEROXIDEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The transition from juvenility over maturation to senescence is a complex process involving multiple transcription factors and regulatory circuits, most of which are currently only vaguely defined. Here we identify JUNGBRUNNEN1 (JUB1), a NAC transcription factor gene rapidly induced by hydrogen peroxide (H2O2), as a central regulator of juvenility in Arabidopsis thaliana. Overexpression of JUB1 under constitutive and inducible promoters strongly delays senescence, enhances tolerance to various abiotic stresses including oxidative stress, and dampens the intracellular H2O2 level. In contrast, precocious senescence and lowered tolerance to abiotic stress were evident in the jub1-1 knock-down line. In vitro binding site selection identified the preferred JUB1 binding site containing RRYGCCGT as its core sequence. The JUB1 binding site is present within the 5´ upstream regulatory region of DREB2A, a transcription factor playing an important role in abiotic stress responses. In accordance with this, JUB1 transactivates DREB2A expression in mesophyll cell protoplasts and transgenic plants and binds in vitro an in vivo to the DREB2A promoter. Transcriptome profiling of JUB1 overexpressors revealed elevated expression of several reactive oxygen species (ROS) responsive genes, including various heat shock protein (HSP) and ROS scavenging glutathione S-transferase (GST) genes, whose expression is further induced by H2O2 treatment. Metabolite profiling revealed that levels of proline and trehalose were significantly elevated in JUB1 overexpression plants, in accordance with their enhanced abiotic stress tolerance. We hypothesize that JUB1 constitutes a central regulator of a finely tuned control system that modulates cellular H2O2 level and primes the plants for upcoming stress through a gene regulatory network that involves DREB2A.Fil: Wu, Anhui. Universitat Potsdam; Alemania. Institut Max Planck fur Molekulare Physiologie; AlemaniaFil: Allu, Annapurna Devi. Universitat Potsdam; Alemania. Institut Max Planck fur Molekulare Physiologie; AlemaniaFil: Garapati, Prashanth. Universitat Potsdam; Alemania. Institut Max Planck fur Molekulare Physiologie; AlemaniaFil: Siddiqui, Hamad. Universitat Potsdam; AlemaniaFil: Dortay, Hakan. Universitat Potsdam; AlemaniaFil: Zanor, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Institut Max Planck fur Molekulare Physiologie; AlemaniaFil: Asensi Fabado, Maria Amparo. Universidad de Barcelona; EspañaFil: Munné Bosch, Sergi. Universidad de Barcelona; EspañaFil: Antonio, Carla. Institut Max Planck fur Molekulare Physiologie; AlemaniaFil: Tohge, Takayuki. Institut Max Planck fur Molekulare Physiologie; AlemaniaFil: Fernie, Alisdair R.. Institut Max Planck fur Molekulare Physiologie; AlemaniaFil: Kaufmann, Kerstin. University of Agriculture Wageningen; Países BajosFil: Xue, Gang-Ping. Commonwealth Scientific And Industrial Research Organisation (csiro);Fil: Mueller Roeber, Bernd. Universitat Potsdam; Alemania. Institut Max Planck fur Molekulare Physiologie; AlemaniaFil: Balazadeh, Salma. Universitat Potsdam; Alemania. Institut Max Planck fur Molekulare Physiologie; AlemaniaAmerican Society of Plant Biologist2012-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/268994Wu, Anhui; Allu, Annapurna Devi; Garapati, Prashanth; Siddiqui, Hamad; Dortay, Hakan; et al.; JUNGBRUNNEN1, a Reactive Oxygen Species–Responsive NAC Transcription Factor, Regulates Longevity in Arabidopsis; American Society of Plant Biologist; Plant Cell; 24; 2; 2-2012; 482-5061040-4651CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/plcell/article/24/2/482/6097129info:eu-repo/semantics/altIdentifier/doi/10.1105/tpc.111.090894info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:10:35Zoai:ri.conicet.gov.ar:11336/268994instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:10:35.73CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
JUNGBRUNNEN1, a Reactive Oxygen Species–Responsive NAC Transcription Factor, Regulates Longevity in Arabidopsis |
title |
JUNGBRUNNEN1, a Reactive Oxygen Species–Responsive NAC Transcription Factor, Regulates Longevity in Arabidopsis |
spellingShingle |
JUNGBRUNNEN1, a Reactive Oxygen Species–Responsive NAC Transcription Factor, Regulates Longevity in Arabidopsis Wu, Anhui ARABIDOPSIS SENESCENCE HYDROGEN PEROXIDE |
title_short |
JUNGBRUNNEN1, a Reactive Oxygen Species–Responsive NAC Transcription Factor, Regulates Longevity in Arabidopsis |
title_full |
JUNGBRUNNEN1, a Reactive Oxygen Species–Responsive NAC Transcription Factor, Regulates Longevity in Arabidopsis |
title_fullStr |
JUNGBRUNNEN1, a Reactive Oxygen Species–Responsive NAC Transcription Factor, Regulates Longevity in Arabidopsis |
title_full_unstemmed |
JUNGBRUNNEN1, a Reactive Oxygen Species–Responsive NAC Transcription Factor, Regulates Longevity in Arabidopsis |
title_sort |
JUNGBRUNNEN1, a Reactive Oxygen Species–Responsive NAC Transcription Factor, Regulates Longevity in Arabidopsis |
dc.creator.none.fl_str_mv |
Wu, Anhui Allu, Annapurna Devi Garapati, Prashanth Siddiqui, Hamad Dortay, Hakan Zanor, María Inés Asensi Fabado, Maria Amparo Munné Bosch, Sergi Antonio, Carla Tohge, Takayuki Fernie, Alisdair R. Kaufmann, Kerstin Xue, Gang-Ping Mueller Roeber, Bernd Balazadeh, Salma |
author |
Wu, Anhui |
author_facet |
Wu, Anhui Allu, Annapurna Devi Garapati, Prashanth Siddiqui, Hamad Dortay, Hakan Zanor, María Inés Asensi Fabado, Maria Amparo Munné Bosch, Sergi Antonio, Carla Tohge, Takayuki Fernie, Alisdair R. Kaufmann, Kerstin Xue, Gang-Ping Mueller Roeber, Bernd Balazadeh, Salma |
author_role |
author |
author2 |
Allu, Annapurna Devi Garapati, Prashanth Siddiqui, Hamad Dortay, Hakan Zanor, María Inés Asensi Fabado, Maria Amparo Munné Bosch, Sergi Antonio, Carla Tohge, Takayuki Fernie, Alisdair R. Kaufmann, Kerstin Xue, Gang-Ping Mueller Roeber, Bernd Balazadeh, Salma |
author2_role |
author author author author author author author author author author author author author author |
dc.subject.none.fl_str_mv |
ARABIDOPSIS SENESCENCE HYDROGEN PEROXIDE |
topic |
ARABIDOPSIS SENESCENCE HYDROGEN PEROXIDE |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The transition from juvenility over maturation to senescence is a complex process involving multiple transcription factors and regulatory circuits, most of which are currently only vaguely defined. Here we identify JUNGBRUNNEN1 (JUB1), a NAC transcription factor gene rapidly induced by hydrogen peroxide (H2O2), as a central regulator of juvenility in Arabidopsis thaliana. Overexpression of JUB1 under constitutive and inducible promoters strongly delays senescence, enhances tolerance to various abiotic stresses including oxidative stress, and dampens the intracellular H2O2 level. In contrast, precocious senescence and lowered tolerance to abiotic stress were evident in the jub1-1 knock-down line. In vitro binding site selection identified the preferred JUB1 binding site containing RRYGCCGT as its core sequence. The JUB1 binding site is present within the 5´ upstream regulatory region of DREB2A, a transcription factor playing an important role in abiotic stress responses. In accordance with this, JUB1 transactivates DREB2A expression in mesophyll cell protoplasts and transgenic plants and binds in vitro an in vivo to the DREB2A promoter. Transcriptome profiling of JUB1 overexpressors revealed elevated expression of several reactive oxygen species (ROS) responsive genes, including various heat shock protein (HSP) and ROS scavenging glutathione S-transferase (GST) genes, whose expression is further induced by H2O2 treatment. Metabolite profiling revealed that levels of proline and trehalose were significantly elevated in JUB1 overexpression plants, in accordance with their enhanced abiotic stress tolerance. We hypothesize that JUB1 constitutes a central regulator of a finely tuned control system that modulates cellular H2O2 level and primes the plants for upcoming stress through a gene regulatory network that involves DREB2A. Fil: Wu, Anhui. Universitat Potsdam; Alemania. Institut Max Planck fur Molekulare Physiologie; Alemania Fil: Allu, Annapurna Devi. Universitat Potsdam; Alemania. Institut Max Planck fur Molekulare Physiologie; Alemania Fil: Garapati, Prashanth. Universitat Potsdam; Alemania. Institut Max Planck fur Molekulare Physiologie; Alemania Fil: Siddiqui, Hamad. Universitat Potsdam; Alemania Fil: Dortay, Hakan. Universitat Potsdam; Alemania Fil: Zanor, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Institut Max Planck fur Molekulare Physiologie; Alemania Fil: Asensi Fabado, Maria Amparo. Universidad de Barcelona; España Fil: Munné Bosch, Sergi. Universidad de Barcelona; España Fil: Antonio, Carla. Institut Max Planck fur Molekulare Physiologie; Alemania Fil: Tohge, Takayuki. Institut Max Planck fur Molekulare Physiologie; Alemania Fil: Fernie, Alisdair R.. Institut Max Planck fur Molekulare Physiologie; Alemania Fil: Kaufmann, Kerstin. University of Agriculture Wageningen; Países Bajos Fil: Xue, Gang-Ping. Commonwealth Scientific And Industrial Research Organisation (csiro); Fil: Mueller Roeber, Bernd. Universitat Potsdam; Alemania. Institut Max Planck fur Molekulare Physiologie; Alemania Fil: Balazadeh, Salma. Universitat Potsdam; Alemania. Institut Max Planck fur Molekulare Physiologie; Alemania |
description |
The transition from juvenility over maturation to senescence is a complex process involving multiple transcription factors and regulatory circuits, most of which are currently only vaguely defined. Here we identify JUNGBRUNNEN1 (JUB1), a NAC transcription factor gene rapidly induced by hydrogen peroxide (H2O2), as a central regulator of juvenility in Arabidopsis thaliana. Overexpression of JUB1 under constitutive and inducible promoters strongly delays senescence, enhances tolerance to various abiotic stresses including oxidative stress, and dampens the intracellular H2O2 level. In contrast, precocious senescence and lowered tolerance to abiotic stress were evident in the jub1-1 knock-down line. In vitro binding site selection identified the preferred JUB1 binding site containing RRYGCCGT as its core sequence. The JUB1 binding site is present within the 5´ upstream regulatory region of DREB2A, a transcription factor playing an important role in abiotic stress responses. In accordance with this, JUB1 transactivates DREB2A expression in mesophyll cell protoplasts and transgenic plants and binds in vitro an in vivo to the DREB2A promoter. Transcriptome profiling of JUB1 overexpressors revealed elevated expression of several reactive oxygen species (ROS) responsive genes, including various heat shock protein (HSP) and ROS scavenging glutathione S-transferase (GST) genes, whose expression is further induced by H2O2 treatment. Metabolite profiling revealed that levels of proline and trehalose were significantly elevated in JUB1 overexpression plants, in accordance with their enhanced abiotic stress tolerance. We hypothesize that JUB1 constitutes a central regulator of a finely tuned control system that modulates cellular H2O2 level and primes the plants for upcoming stress through a gene regulatory network that involves DREB2A. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-02 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/268994 Wu, Anhui; Allu, Annapurna Devi; Garapati, Prashanth; Siddiqui, Hamad; Dortay, Hakan; et al.; JUNGBRUNNEN1, a Reactive Oxygen Species–Responsive NAC Transcription Factor, Regulates Longevity in Arabidopsis; American Society of Plant Biologist; Plant Cell; 24; 2; 2-2012; 482-506 1040-4651 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/268994 |
identifier_str_mv |
Wu, Anhui; Allu, Annapurna Devi; Garapati, Prashanth; Siddiqui, Hamad; Dortay, Hakan; et al.; JUNGBRUNNEN1, a Reactive Oxygen Species–Responsive NAC Transcription Factor, Regulates Longevity in Arabidopsis; American Society of Plant Biologist; Plant Cell; 24; 2; 2-2012; 482-506 1040-4651 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/plcell/article/24/2/482/6097129 info:eu-repo/semantics/altIdentifier/doi/10.1105/tpc.111.090894 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
American Society of Plant Biologist |
publisher.none.fl_str_mv |
American Society of Plant Biologist |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613996100976640 |
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13.070432 |