JUNGBRUNNEN1, a Reactive Oxygen Species–Responsive NAC Transcription Factor, Regulates Longevity in Arabidopsis

Autores
Wu, Anhui; Allu, Annapurna Devi; Garapati, Prashanth; Siddiqui, Hamad; Dortay, Hakan; Zanor, María Inés; Asensi Fabado, Maria Amparo; Munné Bosch, Sergi; Antonio, Carla; Tohge, Takayuki; Fernie, Alisdair R.; Kaufmann, Kerstin; Xue, Gang-Ping; Mueller Roeber, Bernd; Balazadeh, Salma
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The transition from juvenility over maturation to senescence is a complex process involving multiple transcription factors and regulatory circuits, most of which are currently only vaguely defined. Here we identify JUNGBRUNNEN1 (JUB1), a NAC transcription factor gene rapidly induced by hydrogen peroxide (H2O2), as a central regulator of juvenility in Arabidopsis thaliana. Overexpression of JUB1 under constitutive and inducible promoters strongly delays senescence, enhances tolerance to various abiotic stresses including oxidative stress, and dampens the intracellular H2O2 level. In contrast, precocious senescence and lowered tolerance to abiotic stress were evident in the jub1-1 knock-down line. In vitro binding site selection identified the preferred JUB1 binding site containing RRYGCCGT as its core sequence. The JUB1 binding site is present within the 5´ upstream regulatory region of DREB2A, a transcription factor playing an important role in abiotic stress responses. In accordance with this, JUB1 transactivates DREB2A expression in mesophyll cell protoplasts and transgenic plants and binds in vitro an in vivo to the DREB2A promoter. Transcriptome profiling of JUB1 overexpressors revealed elevated expression of several reactive oxygen species (ROS) responsive genes, including various heat shock protein (HSP) and ROS scavenging glutathione S-transferase (GST) genes, whose expression is further induced by H2O2 treatment. Metabolite profiling revealed that levels of proline and trehalose were significantly elevated in JUB1 overexpression plants, in accordance with their enhanced abiotic stress tolerance. We hypothesize that JUB1 constitutes a central regulator of a finely tuned control system that modulates cellular H2O2 level and primes the plants for upcoming stress through a gene regulatory network that involves DREB2A.
Fil: Wu, Anhui. Universitat Potsdam; Alemania. Institut Max Planck fur Molekulare Physiologie; Alemania
Fil: Allu, Annapurna Devi. Universitat Potsdam; Alemania. Institut Max Planck fur Molekulare Physiologie; Alemania
Fil: Garapati, Prashanth. Universitat Potsdam; Alemania. Institut Max Planck fur Molekulare Physiologie; Alemania
Fil: Siddiqui, Hamad. Universitat Potsdam; Alemania
Fil: Dortay, Hakan. Universitat Potsdam; Alemania
Fil: Zanor, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Institut Max Planck fur Molekulare Physiologie; Alemania
Fil: Asensi Fabado, Maria Amparo. Universidad de Barcelona; España
Fil: Munné Bosch, Sergi. Universidad de Barcelona; España
Fil: Antonio, Carla. Institut Max Planck fur Molekulare Physiologie; Alemania
Fil: Tohge, Takayuki. Institut Max Planck fur Molekulare Physiologie; Alemania
Fil: Fernie, Alisdair R.. Institut Max Planck fur Molekulare Physiologie; Alemania
Fil: Kaufmann, Kerstin. University of Agriculture Wageningen; Países Bajos
Fil: Xue, Gang-Ping. Commonwealth Scientific And Industrial Research Organisation (csiro);
Fil: Mueller Roeber, Bernd. Universitat Potsdam; Alemania. Institut Max Planck fur Molekulare Physiologie; Alemania
Fil: Balazadeh, Salma. Universitat Potsdam; Alemania. Institut Max Planck fur Molekulare Physiologie; Alemania
Materia
ARABIDOPSIS
SENESCENCE
HYDROGEN PEROXIDE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/268994

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spelling JUNGBRUNNEN1, a Reactive Oxygen Species–Responsive NAC Transcription Factor, Regulates Longevity in ArabidopsisWu, AnhuiAllu, Annapurna DeviGarapati, PrashanthSiddiqui, HamadDortay, HakanZanor, María InésAsensi Fabado, Maria AmparoMunné Bosch, SergiAntonio, CarlaTohge, TakayukiFernie, Alisdair R.Kaufmann, KerstinXue, Gang-PingMueller Roeber, BerndBalazadeh, SalmaARABIDOPSISSENESCENCEHYDROGEN PEROXIDEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The transition from juvenility over maturation to senescence is a complex process involving multiple transcription factors and regulatory circuits, most of which are currently only vaguely defined. Here we identify JUNGBRUNNEN1 (JUB1), a NAC transcription factor gene rapidly induced by hydrogen peroxide (H2O2), as a central regulator of juvenility in Arabidopsis thaliana. Overexpression of JUB1 under constitutive and inducible promoters strongly delays senescence, enhances tolerance to various abiotic stresses including oxidative stress, and dampens the intracellular H2O2 level. In contrast, precocious senescence and lowered tolerance to abiotic stress were evident in the jub1-1 knock-down line. In vitro binding site selection identified the preferred JUB1 binding site containing RRYGCCGT as its core sequence. The JUB1 binding site is present within the 5´ upstream regulatory region of DREB2A, a transcription factor playing an important role in abiotic stress responses. In accordance with this, JUB1 transactivates DREB2A expression in mesophyll cell protoplasts and transgenic plants and binds in vitro an in vivo to the DREB2A promoter. Transcriptome profiling of JUB1 overexpressors revealed elevated expression of several reactive oxygen species (ROS) responsive genes, including various heat shock protein (HSP) and ROS scavenging glutathione S-transferase (GST) genes, whose expression is further induced by H2O2 treatment. Metabolite profiling revealed that levels of proline and trehalose were significantly elevated in JUB1 overexpression plants, in accordance with their enhanced abiotic stress tolerance. We hypothesize that JUB1 constitutes a central regulator of a finely tuned control system that modulates cellular H2O2 level and primes the plants for upcoming stress through a gene regulatory network that involves DREB2A.Fil: Wu, Anhui. Universitat Potsdam; Alemania. Institut Max Planck fur Molekulare Physiologie; AlemaniaFil: Allu, Annapurna Devi. Universitat Potsdam; Alemania. Institut Max Planck fur Molekulare Physiologie; AlemaniaFil: Garapati, Prashanth. Universitat Potsdam; Alemania. Institut Max Planck fur Molekulare Physiologie; AlemaniaFil: Siddiqui, Hamad. Universitat Potsdam; AlemaniaFil: Dortay, Hakan. Universitat Potsdam; AlemaniaFil: Zanor, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Institut Max Planck fur Molekulare Physiologie; AlemaniaFil: Asensi Fabado, Maria Amparo. Universidad de Barcelona; EspañaFil: Munné Bosch, Sergi. Universidad de Barcelona; EspañaFil: Antonio, Carla. Institut Max Planck fur Molekulare Physiologie; AlemaniaFil: Tohge, Takayuki. Institut Max Planck fur Molekulare Physiologie; AlemaniaFil: Fernie, Alisdair R.. Institut Max Planck fur Molekulare Physiologie; AlemaniaFil: Kaufmann, Kerstin. University of Agriculture Wageningen; Países BajosFil: Xue, Gang-Ping. Commonwealth Scientific And Industrial Research Organisation (csiro);Fil: Mueller Roeber, Bernd. Universitat Potsdam; Alemania. Institut Max Planck fur Molekulare Physiologie; AlemaniaFil: Balazadeh, Salma. Universitat Potsdam; Alemania. Institut Max Planck fur Molekulare Physiologie; AlemaniaAmerican Society of Plant Biologist2012-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/268994Wu, Anhui; Allu, Annapurna Devi; Garapati, Prashanth; Siddiqui, Hamad; Dortay, Hakan; et al.; JUNGBRUNNEN1, a Reactive Oxygen Species–Responsive NAC Transcription Factor, Regulates Longevity in Arabidopsis; American Society of Plant Biologist; Plant Cell; 24; 2; 2-2012; 482-5061040-4651CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/plcell/article/24/2/482/6097129info:eu-repo/semantics/altIdentifier/doi/10.1105/tpc.111.090894info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:10:35Zoai:ri.conicet.gov.ar:11336/268994instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:10:35.73CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv JUNGBRUNNEN1, a Reactive Oxygen Species–Responsive NAC Transcription Factor, Regulates Longevity in Arabidopsis
title JUNGBRUNNEN1, a Reactive Oxygen Species–Responsive NAC Transcription Factor, Regulates Longevity in Arabidopsis
spellingShingle JUNGBRUNNEN1, a Reactive Oxygen Species–Responsive NAC Transcription Factor, Regulates Longevity in Arabidopsis
Wu, Anhui
ARABIDOPSIS
SENESCENCE
HYDROGEN PEROXIDE
title_short JUNGBRUNNEN1, a Reactive Oxygen Species–Responsive NAC Transcription Factor, Regulates Longevity in Arabidopsis
title_full JUNGBRUNNEN1, a Reactive Oxygen Species–Responsive NAC Transcription Factor, Regulates Longevity in Arabidopsis
title_fullStr JUNGBRUNNEN1, a Reactive Oxygen Species–Responsive NAC Transcription Factor, Regulates Longevity in Arabidopsis
title_full_unstemmed JUNGBRUNNEN1, a Reactive Oxygen Species–Responsive NAC Transcription Factor, Regulates Longevity in Arabidopsis
title_sort JUNGBRUNNEN1, a Reactive Oxygen Species–Responsive NAC Transcription Factor, Regulates Longevity in Arabidopsis
dc.creator.none.fl_str_mv Wu, Anhui
Allu, Annapurna Devi
Garapati, Prashanth
Siddiqui, Hamad
Dortay, Hakan
Zanor, María Inés
Asensi Fabado, Maria Amparo
Munné Bosch, Sergi
Antonio, Carla
Tohge, Takayuki
Fernie, Alisdair R.
Kaufmann, Kerstin
Xue, Gang-Ping
Mueller Roeber, Bernd
Balazadeh, Salma
author Wu, Anhui
author_facet Wu, Anhui
Allu, Annapurna Devi
Garapati, Prashanth
Siddiqui, Hamad
Dortay, Hakan
Zanor, María Inés
Asensi Fabado, Maria Amparo
Munné Bosch, Sergi
Antonio, Carla
Tohge, Takayuki
Fernie, Alisdair R.
Kaufmann, Kerstin
Xue, Gang-Ping
Mueller Roeber, Bernd
Balazadeh, Salma
author_role author
author2 Allu, Annapurna Devi
Garapati, Prashanth
Siddiqui, Hamad
Dortay, Hakan
Zanor, María Inés
Asensi Fabado, Maria Amparo
Munné Bosch, Sergi
Antonio, Carla
Tohge, Takayuki
Fernie, Alisdair R.
Kaufmann, Kerstin
Xue, Gang-Ping
Mueller Roeber, Bernd
Balazadeh, Salma
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ARABIDOPSIS
SENESCENCE
HYDROGEN PEROXIDE
topic ARABIDOPSIS
SENESCENCE
HYDROGEN PEROXIDE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The transition from juvenility over maturation to senescence is a complex process involving multiple transcription factors and regulatory circuits, most of which are currently only vaguely defined. Here we identify JUNGBRUNNEN1 (JUB1), a NAC transcription factor gene rapidly induced by hydrogen peroxide (H2O2), as a central regulator of juvenility in Arabidopsis thaliana. Overexpression of JUB1 under constitutive and inducible promoters strongly delays senescence, enhances tolerance to various abiotic stresses including oxidative stress, and dampens the intracellular H2O2 level. In contrast, precocious senescence and lowered tolerance to abiotic stress were evident in the jub1-1 knock-down line. In vitro binding site selection identified the preferred JUB1 binding site containing RRYGCCGT as its core sequence. The JUB1 binding site is present within the 5´ upstream regulatory region of DREB2A, a transcription factor playing an important role in abiotic stress responses. In accordance with this, JUB1 transactivates DREB2A expression in mesophyll cell protoplasts and transgenic plants and binds in vitro an in vivo to the DREB2A promoter. Transcriptome profiling of JUB1 overexpressors revealed elevated expression of several reactive oxygen species (ROS) responsive genes, including various heat shock protein (HSP) and ROS scavenging glutathione S-transferase (GST) genes, whose expression is further induced by H2O2 treatment. Metabolite profiling revealed that levels of proline and trehalose were significantly elevated in JUB1 overexpression plants, in accordance with their enhanced abiotic stress tolerance. We hypothesize that JUB1 constitutes a central regulator of a finely tuned control system that modulates cellular H2O2 level and primes the plants for upcoming stress through a gene regulatory network that involves DREB2A.
Fil: Wu, Anhui. Universitat Potsdam; Alemania. Institut Max Planck fur Molekulare Physiologie; Alemania
Fil: Allu, Annapurna Devi. Universitat Potsdam; Alemania. Institut Max Planck fur Molekulare Physiologie; Alemania
Fil: Garapati, Prashanth. Universitat Potsdam; Alemania. Institut Max Planck fur Molekulare Physiologie; Alemania
Fil: Siddiqui, Hamad. Universitat Potsdam; Alemania
Fil: Dortay, Hakan. Universitat Potsdam; Alemania
Fil: Zanor, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Institut Max Planck fur Molekulare Physiologie; Alemania
Fil: Asensi Fabado, Maria Amparo. Universidad de Barcelona; España
Fil: Munné Bosch, Sergi. Universidad de Barcelona; España
Fil: Antonio, Carla. Institut Max Planck fur Molekulare Physiologie; Alemania
Fil: Tohge, Takayuki. Institut Max Planck fur Molekulare Physiologie; Alemania
Fil: Fernie, Alisdair R.. Institut Max Planck fur Molekulare Physiologie; Alemania
Fil: Kaufmann, Kerstin. University of Agriculture Wageningen; Países Bajos
Fil: Xue, Gang-Ping. Commonwealth Scientific And Industrial Research Organisation (csiro);
Fil: Mueller Roeber, Bernd. Universitat Potsdam; Alemania. Institut Max Planck fur Molekulare Physiologie; Alemania
Fil: Balazadeh, Salma. Universitat Potsdam; Alemania. Institut Max Planck fur Molekulare Physiologie; Alemania
description The transition from juvenility over maturation to senescence is a complex process involving multiple transcription factors and regulatory circuits, most of which are currently only vaguely defined. Here we identify JUNGBRUNNEN1 (JUB1), a NAC transcription factor gene rapidly induced by hydrogen peroxide (H2O2), as a central regulator of juvenility in Arabidopsis thaliana. Overexpression of JUB1 under constitutive and inducible promoters strongly delays senescence, enhances tolerance to various abiotic stresses including oxidative stress, and dampens the intracellular H2O2 level. In contrast, precocious senescence and lowered tolerance to abiotic stress were evident in the jub1-1 knock-down line. In vitro binding site selection identified the preferred JUB1 binding site containing RRYGCCGT as its core sequence. The JUB1 binding site is present within the 5´ upstream regulatory region of DREB2A, a transcription factor playing an important role in abiotic stress responses. In accordance with this, JUB1 transactivates DREB2A expression in mesophyll cell protoplasts and transgenic plants and binds in vitro an in vivo to the DREB2A promoter. Transcriptome profiling of JUB1 overexpressors revealed elevated expression of several reactive oxygen species (ROS) responsive genes, including various heat shock protein (HSP) and ROS scavenging glutathione S-transferase (GST) genes, whose expression is further induced by H2O2 treatment. Metabolite profiling revealed that levels of proline and trehalose were significantly elevated in JUB1 overexpression plants, in accordance with their enhanced abiotic stress tolerance. We hypothesize that JUB1 constitutes a central regulator of a finely tuned control system that modulates cellular H2O2 level and primes the plants for upcoming stress through a gene regulatory network that involves DREB2A.
publishDate 2012
dc.date.none.fl_str_mv 2012-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/268994
Wu, Anhui; Allu, Annapurna Devi; Garapati, Prashanth; Siddiqui, Hamad; Dortay, Hakan; et al.; JUNGBRUNNEN1, a Reactive Oxygen Species–Responsive NAC Transcription Factor, Regulates Longevity in Arabidopsis; American Society of Plant Biologist; Plant Cell; 24; 2; 2-2012; 482-506
1040-4651
CONICET Digital
CONICET
url http://hdl.handle.net/11336/268994
identifier_str_mv Wu, Anhui; Allu, Annapurna Devi; Garapati, Prashanth; Siddiqui, Hamad; Dortay, Hakan; et al.; JUNGBRUNNEN1, a Reactive Oxygen Species–Responsive NAC Transcription Factor, Regulates Longevity in Arabidopsis; American Society of Plant Biologist; Plant Cell; 24; 2; 2-2012; 482-506
1040-4651
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/plcell/article/24/2/482/6097129
info:eu-repo/semantics/altIdentifier/doi/10.1105/tpc.111.090894
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Plant Biologist
publisher.none.fl_str_mv American Society of Plant Biologist
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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