Genome-wide and comparative phylogenetic analysis of senescence-associated NAC transcription factors in sunflower (Helianthus annuus)

Autores
Bengoa Luoni, Sofía Ailin; Cenci, Alberto; Moschen, Sebastian Nicolas; Nicosia, Salvador Maria; Radonic, Laura Mabel; Sabio Y Garcia, Julia Veronica; Langlade, Nicolas B.; Vile, Denis; Vazquez Rovere, Cecilia; Fernandez, Paula Del Carmen
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Leaf senescence delay impacts positively in grain yield by maintaining the photosynthetic area during the reproductive stage and during grain filling. Therefore a comprehensive understanding of the gene families associated with leaf senescence is essential. NAC transcription factors (TF) form a large plant-specific gene family involved in regulating development, senescence, and responses to biotic and abiotic stresses. The main goal of this work was to identify sunflower NAC TF (HaNAC) and their association with senescence, studying their orthologous to understand possible functional relationships between genes of different species. Results: To clarify the orthologous relationships, we used an in-depth comparative study of four divergent taxa, in dicots and monocots, with completely sequenced genomes (Arabidopsis thaliana, Vitis vinifera, Musa acuminata and Oryza sativa). These orthologous groups provide a curated resource for large scale protein sequence annotation of NAC TF. From the 151 HaNAC genes detected in the latest version of the sunflower genome, 50 genes were associated with senescence traits. These genes showed significant differential expression in two contrasting lines according to an RNAseq assay. An assessment of overexpressing the Arabidopsis line for HaNAC001 (a gene of the same orthologous group of Arabidopsis thaliana ORE1) revealed that this line displayed a significantly higher number of senescent leaves and a pronounced change in development rate. Conclusions: This finding suggests HaNAC001 as an interesting candidate to explore the molecular regulation of senescence in sunflower.
Instituto de Biotecnología
Fil: Bengoa Luoni, Sofia Ailin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina.
Fil: Cenci, Alberto. Bioversity International; Francia
Fil: Moschen, Sebastian Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina.
Fil: Nicosia, Salvador. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina.
Fil: Radonic, Laura Mabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina.
Fil: Sabio Y Garcia, Julia Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Langlade, Nicolas B. Université de Toulouse. LIPM-INRA-CNRS; Francia
Fil: Langlade, Nicolas B. Université de Montpellier. LEPSE; Francia
Fil: Vazquez Rovere, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Fernandez, Paula Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina.
Fuente
BMC Genomics 22 : Article number: 893 (2021)
Materia
Helianthus annuus
Avejentamiento
Genética
Factores de Transcripción
Análisis Filogenético
Senescence
Genetics
Transcription Factors
Phylogenetic Analysis
Leaves
Hojas
Girasol
Sunflower
Leaf Senescence
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/11022
Acceder
id INTADig_ac006e4fc9460f3e1fff91eafd454a6c
oai_identifier_str oai:localhost:20.500.12123/11022
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Genome-wide and comparative phylogenetic analysis of senescence-associated NAC transcription factors in sunflower (Helianthus annuus)Bengoa Luoni, Sofía AilinCenci, AlbertoMoschen, Sebastian NicolasNicosia, Salvador MariaRadonic, Laura MabelSabio Y Garcia, Julia VeronicaLanglade, Nicolas B.Vile, DenisVazquez Rovere, CeciliaFernandez, Paula Del CarmenHelianthus annuusAvejentamientoGenéticaFactores de TranscripciónAnálisis FilogenéticoSenescenceGeneticsTranscription FactorsPhylogenetic AnalysisLeavesHojasGirasolSunflowerLeaf SenescenceBackground: Leaf senescence delay impacts positively in grain yield by maintaining the photosynthetic area during the reproductive stage and during grain filling. Therefore a comprehensive understanding of the gene families associated with leaf senescence is essential. NAC transcription factors (TF) form a large plant-specific gene family involved in regulating development, senescence, and responses to biotic and abiotic stresses. The main goal of this work was to identify sunflower NAC TF (HaNAC) and their association with senescence, studying their orthologous to understand possible functional relationships between genes of different species. Results: To clarify the orthologous relationships, we used an in-depth comparative study of four divergent taxa, in dicots and monocots, with completely sequenced genomes (Arabidopsis thaliana, Vitis vinifera, Musa acuminata and Oryza sativa). These orthologous groups provide a curated resource for large scale protein sequence annotation of NAC TF. From the 151 HaNAC genes detected in the latest version of the sunflower genome, 50 genes were associated with senescence traits. These genes showed significant differential expression in two contrasting lines according to an RNAseq assay. An assessment of overexpressing the Arabidopsis line for HaNAC001 (a gene of the same orthologous group of Arabidopsis thaliana ORE1) revealed that this line displayed a significantly higher number of senescent leaves and a pronounced change in development rate. Conclusions: This finding suggests HaNAC001 as an interesting candidate to explore the molecular regulation of senescence in sunflower.Instituto de BiotecnologíaFil: Bengoa Luoni, Sofia Ailin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina.Fil: Cenci, Alberto. Bioversity International; FranciaFil: Moschen, Sebastian Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina.Fil: Nicosia, Salvador. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina.Fil: Radonic, Laura Mabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina.Fil: Sabio Y Garcia, Julia Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Langlade, Nicolas B. Université de Toulouse. LIPM-INRA-CNRS; FranciaFil: Langlade, Nicolas B. Université de Montpellier. LEPSE; FranciaFil: Vazquez Rovere, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Fernandez, Paula Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina.Springer2021-12-30T12:35:10Z2021-12-30T12:35:10Z2021-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/11022https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-021-08199-51471-2164https://doi.org/10.1186/s12864-021-08199-5BMC Genomics 22 : Article number: 893 (2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNBIO-1131022/AR./Genómica funcional y biología de sistemas.info:eu-repograntAgreement/INTA/PNBIO-1131043/AR./Bioinformática y Estadística Genómica.info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:49:14Zoai:localhost:20.500.12123/11022instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:49:14.442INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Genome-wide and comparative phylogenetic analysis of senescence-associated NAC transcription factors in sunflower (Helianthus annuus)
title Genome-wide and comparative phylogenetic analysis of senescence-associated NAC transcription factors in sunflower (Helianthus annuus)
spellingShingle Genome-wide and comparative phylogenetic analysis of senescence-associated NAC transcription factors in sunflower (Helianthus annuus)
Bengoa Luoni, Sofía Ailin
Helianthus annuus
Avejentamiento
Genética
Factores de Transcripción
Análisis Filogenético
Senescence
Genetics
Transcription Factors
Phylogenetic Analysis
Leaves
Hojas
Girasol
Sunflower
Leaf Senescence
title_short Genome-wide and comparative phylogenetic analysis of senescence-associated NAC transcription factors in sunflower (Helianthus annuus)
title_full Genome-wide and comparative phylogenetic analysis of senescence-associated NAC transcription factors in sunflower (Helianthus annuus)
title_fullStr Genome-wide and comparative phylogenetic analysis of senescence-associated NAC transcription factors in sunflower (Helianthus annuus)
title_full_unstemmed Genome-wide and comparative phylogenetic analysis of senescence-associated NAC transcription factors in sunflower (Helianthus annuus)
title_sort Genome-wide and comparative phylogenetic analysis of senescence-associated NAC transcription factors in sunflower (Helianthus annuus)
dc.creator.none.fl_str_mv Bengoa Luoni, Sofía Ailin
Cenci, Alberto
Moschen, Sebastian Nicolas
Nicosia, Salvador Maria
Radonic, Laura Mabel
Sabio Y Garcia, Julia Veronica
Langlade, Nicolas B.
Vile, Denis
Vazquez Rovere, Cecilia
Fernandez, Paula Del Carmen
author Bengoa Luoni, Sofía Ailin
author_facet Bengoa Luoni, Sofía Ailin
Cenci, Alberto
Moschen, Sebastian Nicolas
Nicosia, Salvador Maria
Radonic, Laura Mabel
Sabio Y Garcia, Julia Veronica
Langlade, Nicolas B.
Vile, Denis
Vazquez Rovere, Cecilia
Fernandez, Paula Del Carmen
author_role author
author2 Cenci, Alberto
Moschen, Sebastian Nicolas
Nicosia, Salvador Maria
Radonic, Laura Mabel
Sabio Y Garcia, Julia Veronica
Langlade, Nicolas B.
Vile, Denis
Vazquez Rovere, Cecilia
Fernandez, Paula Del Carmen
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Helianthus annuus
Avejentamiento
Genética
Factores de Transcripción
Análisis Filogenético
Senescence
Genetics
Transcription Factors
Phylogenetic Analysis
Leaves
Hojas
Girasol
Sunflower
Leaf Senescence
topic Helianthus annuus
Avejentamiento
Genética
Factores de Transcripción
Análisis Filogenético
Senescence
Genetics
Transcription Factors
Phylogenetic Analysis
Leaves
Hojas
Girasol
Sunflower
Leaf Senescence
dc.description.none.fl_txt_mv Background: Leaf senescence delay impacts positively in grain yield by maintaining the photosynthetic area during the reproductive stage and during grain filling. Therefore a comprehensive understanding of the gene families associated with leaf senescence is essential. NAC transcription factors (TF) form a large plant-specific gene family involved in regulating development, senescence, and responses to biotic and abiotic stresses. The main goal of this work was to identify sunflower NAC TF (HaNAC) and their association with senescence, studying their orthologous to understand possible functional relationships between genes of different species. Results: To clarify the orthologous relationships, we used an in-depth comparative study of four divergent taxa, in dicots and monocots, with completely sequenced genomes (Arabidopsis thaliana, Vitis vinifera, Musa acuminata and Oryza sativa). These orthologous groups provide a curated resource for large scale protein sequence annotation of NAC TF. From the 151 HaNAC genes detected in the latest version of the sunflower genome, 50 genes were associated with senescence traits. These genes showed significant differential expression in two contrasting lines according to an RNAseq assay. An assessment of overexpressing the Arabidopsis line for HaNAC001 (a gene of the same orthologous group of Arabidopsis thaliana ORE1) revealed that this line displayed a significantly higher number of senescent leaves and a pronounced change in development rate. Conclusions: This finding suggests HaNAC001 as an interesting candidate to explore the molecular regulation of senescence in sunflower.
Instituto de Biotecnología
Fil: Bengoa Luoni, Sofia Ailin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina.
Fil: Cenci, Alberto. Bioversity International; Francia
Fil: Moschen, Sebastian Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina.
Fil: Nicosia, Salvador. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina.
Fil: Radonic, Laura Mabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina.
Fil: Sabio Y Garcia, Julia Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Langlade, Nicolas B. Université de Toulouse. LIPM-INRA-CNRS; Francia
Fil: Langlade, Nicolas B. Université de Montpellier. LEPSE; Francia
Fil: Vazquez Rovere, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Fernandez, Paula Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina.
description Background: Leaf senescence delay impacts positively in grain yield by maintaining the photosynthetic area during the reproductive stage and during grain filling. Therefore a comprehensive understanding of the gene families associated with leaf senescence is essential. NAC transcription factors (TF) form a large plant-specific gene family involved in regulating development, senescence, and responses to biotic and abiotic stresses. The main goal of this work was to identify sunflower NAC TF (HaNAC) and their association with senescence, studying their orthologous to understand possible functional relationships between genes of different species. Results: To clarify the orthologous relationships, we used an in-depth comparative study of four divergent taxa, in dicots and monocots, with completely sequenced genomes (Arabidopsis thaliana, Vitis vinifera, Musa acuminata and Oryza sativa). These orthologous groups provide a curated resource for large scale protein sequence annotation of NAC TF. From the 151 HaNAC genes detected in the latest version of the sunflower genome, 50 genes were associated with senescence traits. These genes showed significant differential expression in two contrasting lines according to an RNAseq assay. An assessment of overexpressing the Arabidopsis line for HaNAC001 (a gene of the same orthologous group of Arabidopsis thaliana ORE1) revealed that this line displayed a significantly higher number of senescent leaves and a pronounced change in development rate. Conclusions: This finding suggests HaNAC001 as an interesting candidate to explore the molecular regulation of senescence in sunflower.
publishDate 2021
dc.date.none.fl_str_mv 2021-12-30T12:35:10Z
2021-12-30T12:35:10Z
2021-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/11022
https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-021-08199-5
1471-2164
https://doi.org/10.1186/s12864-021-08199-5
url http://hdl.handle.net/20.500.12123/11022
https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-021-08199-5
https://doi.org/10.1186/s12864-021-08199-5
identifier_str_mv 1471-2164
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNBIO-1131022/AR./Genómica funcional y biología de sistemas.
info:eu-repograntAgreement/INTA/PNBIO-1131043/AR./Bioinformática y Estadística Genómica.
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv BMC Genomics 22 : Article number: 893 (2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1842341393338466304
score 12.623145

Publicaciones similares