Synthesis and Characterization of B4C-Based Multifunctional Nanoparticles for Boron Neutron Capture Therapy Applications

Autores
Demichelis, Maria Paola; Portu, Agustina Mariana; Gadan, Mario Alberto; Vitali, Agostina; Forlingieri, Valentina; Bortolussi, Silva; Postuma, Ian; Falqui, Andrea; Vezzoli, Elena; Milanese, Chiara; Sommi, Patrizia; Anselmi Tamburini, Umberto
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Nanoparticles composed of inorganic boron-containing compounds represent a promis-ing candidate as 10B carriers for BNCT. This study focuses on the synthesis, characterization, and assessment of the biological activity of composite nanomaterials based on boron carbide (B4C).Boron carbide is a compelling alternative to borated molecules due to its high volumetric B content,prolonged retention in biological systems, and low toxicity. These attributes lead to a substantialaccumulation of B in tissues, eliminating the need for isotopically enriched compounds. In our approach, B4C nanoparticles were included in composite nanostructures with ultrasmall superpara-magnetic nanoparticles (SPIONs), coated with poly (acrylic acid), and further functionalized with the fluorophore DiI. The successful internalization of these nanoparticles in HeLa cells was confirmed, and a significant uptake of 10B was observed. Micro-distribution studies were conducted using intracellular neutron autoradiography, providing valuable insights into the spatial distribution of the nanoparticles within cells. These findings strongly indicate that the developed nanomaterials hold significant promise as effective carriers for 10B in BNCT, showcasing their potential for advancing cancer treatment methodologies.
Fil: Demichelis, Maria Paola. Universita degli Studi di Pavia; Italia
Fil: Portu, Agustina Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Gerencia de Radiobiología (Centro Atómico Constituyentes); Argentina
Fil: Gadan, Mario Alberto. Comisión Nacional de Energía Atómica; Argentina
Fil: Vitali, Agostina. Universita degli Studi di Pavia; Italia
Fil: Forlingieri, Valentina. Universita degli Studi di Pavia; Italia
Fil: Bortolussi, Silva. Istituto Nazionale Di Fisica Nucleare.; Italia
Fil: Postuma, Ian. Universita degli Studi di Pavia; Italia
Fil: Falqui, Andrea. Università degli Studi di Milano; Italia
Fil: Vezzoli, Elena. No especifíca;
Fil: Milanese, Chiara. Universita degli Studi di Pavia; Italia
Fil: Sommi, Patrizia. Universita degli Studi di Pavia; Italia
Fil: Anselmi Tamburini, Umberto. Universita degli Studi di Pavia; Italia
Materia
NANOMATERIALS
BORON CARBIDE
BNCT
NEUTRON AUTORADIOGRAPHY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/236637
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/236637
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Synthesis and Characterization of B4C-Based Multifunctional Nanoparticles for Boron Neutron Capture Therapy ApplicationsDemichelis, Maria PaolaPortu, Agustina MarianaGadan, Mario AlbertoVitali, AgostinaForlingieri, ValentinaBortolussi, SilvaPostuma, IanFalqui, AndreaVezzoli, ElenaMilanese, ChiaraSommi, PatriziaAnselmi Tamburini, UmbertoNANOMATERIALSBORON CARBIDEBNCTNEUTRON AUTORADIOGRAPHYhttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Nanoparticles composed of inorganic boron-containing compounds represent a promis-ing candidate as 10B carriers for BNCT. This study focuses on the synthesis, characterization, and assessment of the biological activity of composite nanomaterials based on boron carbide (B4C).Boron carbide is a compelling alternative to borated molecules due to its high volumetric B content,prolonged retention in biological systems, and low toxicity. These attributes lead to a substantialaccumulation of B in tissues, eliminating the need for isotopically enriched compounds. In our approach, B4C nanoparticles were included in composite nanostructures with ultrasmall superpara-magnetic nanoparticles (SPIONs), coated with poly (acrylic acid), and further functionalized with the fluorophore DiI. The successful internalization of these nanoparticles in HeLa cells was confirmed, and a significant uptake of 10B was observed. Micro-distribution studies were conducted using intracellular neutron autoradiography, providing valuable insights into the spatial distribution of the nanoparticles within cells. These findings strongly indicate that the developed nanomaterials hold significant promise as effective carriers for 10B in BNCT, showcasing their potential for advancing cancer treatment methodologies.Fil: Demichelis, Maria Paola. Universita degli Studi di Pavia; ItaliaFil: Portu, Agustina Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Gerencia de Radiobiología (Centro Atómico Constituyentes); ArgentinaFil: Gadan, Mario Alberto. Comisión Nacional de Energía Atómica; ArgentinaFil: Vitali, Agostina. Universita degli Studi di Pavia; ItaliaFil: Forlingieri, Valentina. Universita degli Studi di Pavia; ItaliaFil: Bortolussi, Silva. Istituto Nazionale Di Fisica Nucleare.; ItaliaFil: Postuma, Ian. Universita degli Studi di Pavia; ItaliaFil: Falqui, Andrea. Università degli Studi di Milano; ItaliaFil: Vezzoli, Elena. No especifíca;Fil: Milanese, Chiara. Universita degli Studi di Pavia; ItaliaFil: Sommi, Patrizia. Universita degli Studi di Pavia; ItaliaFil: Anselmi Tamburini, Umberto. Universita degli Studi di Pavia; ItaliaMDPI2024-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/236637Demichelis, Maria Paola; Portu, Agustina Mariana; Gadan, Mario Alberto; Vitali, Agostina; Forlingieri, Valentina; et al.; Synthesis and Characterization of B4C-Based Multifunctional Nanoparticles for Boron Neutron Capture Therapy Applications; MDPI; Applied Nano; 5; 2; 3-2024; 33-472673-3501CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2673-3501/5/2/4info:eu-repo/semantics/altIdentifier/doi/10.3390/applnano5020004info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:21:19Zoai:ri.conicet.gov.ar:11336/236637instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:21:20.177CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Synthesis and Characterization of B4C-Based Multifunctional Nanoparticles for Boron Neutron Capture Therapy Applications
title Synthesis and Characterization of B4C-Based Multifunctional Nanoparticles for Boron Neutron Capture Therapy Applications
spellingShingle Synthesis and Characterization of B4C-Based Multifunctional Nanoparticles for Boron Neutron Capture Therapy Applications
Demichelis, Maria Paola
NANOMATERIALS
BORON CARBIDE
BNCT
NEUTRON AUTORADIOGRAPHY
title_short Synthesis and Characterization of B4C-Based Multifunctional Nanoparticles for Boron Neutron Capture Therapy Applications
title_full Synthesis and Characterization of B4C-Based Multifunctional Nanoparticles for Boron Neutron Capture Therapy Applications
title_fullStr Synthesis and Characterization of B4C-Based Multifunctional Nanoparticles for Boron Neutron Capture Therapy Applications
title_full_unstemmed Synthesis and Characterization of B4C-Based Multifunctional Nanoparticles for Boron Neutron Capture Therapy Applications
title_sort Synthesis and Characterization of B4C-Based Multifunctional Nanoparticles for Boron Neutron Capture Therapy Applications
dc.creator.none.fl_str_mv Demichelis, Maria Paola
Portu, Agustina Mariana
Gadan, Mario Alberto
Vitali, Agostina
Forlingieri, Valentina
Bortolussi, Silva
Postuma, Ian
Falqui, Andrea
Vezzoli, Elena
Milanese, Chiara
Sommi, Patrizia
Anselmi Tamburini, Umberto
author Demichelis, Maria Paola
author_facet Demichelis, Maria Paola
Portu, Agustina Mariana
Gadan, Mario Alberto
Vitali, Agostina
Forlingieri, Valentina
Bortolussi, Silva
Postuma, Ian
Falqui, Andrea
Vezzoli, Elena
Milanese, Chiara
Sommi, Patrizia
Anselmi Tamburini, Umberto
author_role author
author2 Portu, Agustina Mariana
Gadan, Mario Alberto
Vitali, Agostina
Forlingieri, Valentina
Bortolussi, Silva
Postuma, Ian
Falqui, Andrea
Vezzoli, Elena
Milanese, Chiara
Sommi, Patrizia
Anselmi Tamburini, Umberto
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv NANOMATERIALS
BORON CARBIDE
BNCT
NEUTRON AUTORADIOGRAPHY
topic NANOMATERIALS
BORON CARBIDE
BNCT
NEUTRON AUTORADIOGRAPHY
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Nanoparticles composed of inorganic boron-containing compounds represent a promis-ing candidate as 10B carriers for BNCT. This study focuses on the synthesis, characterization, and assessment of the biological activity of composite nanomaterials based on boron carbide (B4C).Boron carbide is a compelling alternative to borated molecules due to its high volumetric B content,prolonged retention in biological systems, and low toxicity. These attributes lead to a substantialaccumulation of B in tissues, eliminating the need for isotopically enriched compounds. In our approach, B4C nanoparticles were included in composite nanostructures with ultrasmall superpara-magnetic nanoparticles (SPIONs), coated with poly (acrylic acid), and further functionalized with the fluorophore DiI. The successful internalization of these nanoparticles in HeLa cells was confirmed, and a significant uptake of 10B was observed. Micro-distribution studies were conducted using intracellular neutron autoradiography, providing valuable insights into the spatial distribution of the nanoparticles within cells. These findings strongly indicate that the developed nanomaterials hold significant promise as effective carriers for 10B in BNCT, showcasing their potential for advancing cancer treatment methodologies.
Fil: Demichelis, Maria Paola. Universita degli Studi di Pavia; Italia
Fil: Portu, Agustina Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Gerencia de Radiobiología (Centro Atómico Constituyentes); Argentina
Fil: Gadan, Mario Alberto. Comisión Nacional de Energía Atómica; Argentina
Fil: Vitali, Agostina. Universita degli Studi di Pavia; Italia
Fil: Forlingieri, Valentina. Universita degli Studi di Pavia; Italia
Fil: Bortolussi, Silva. Istituto Nazionale Di Fisica Nucleare.; Italia
Fil: Postuma, Ian. Universita degli Studi di Pavia; Italia
Fil: Falqui, Andrea. Università degli Studi di Milano; Italia
Fil: Vezzoli, Elena. No especifíca;
Fil: Milanese, Chiara. Universita degli Studi di Pavia; Italia
Fil: Sommi, Patrizia. Universita degli Studi di Pavia; Italia
Fil: Anselmi Tamburini, Umberto. Universita degli Studi di Pavia; Italia
description Nanoparticles composed of inorganic boron-containing compounds represent a promis-ing candidate as 10B carriers for BNCT. This study focuses on the synthesis, characterization, and assessment of the biological activity of composite nanomaterials based on boron carbide (B4C).Boron carbide is a compelling alternative to borated molecules due to its high volumetric B content,prolonged retention in biological systems, and low toxicity. These attributes lead to a substantialaccumulation of B in tissues, eliminating the need for isotopically enriched compounds. In our approach, B4C nanoparticles were included in composite nanostructures with ultrasmall superpara-magnetic nanoparticles (SPIONs), coated with poly (acrylic acid), and further functionalized with the fluorophore DiI. The successful internalization of these nanoparticles in HeLa cells was confirmed, and a significant uptake of 10B was observed. Micro-distribution studies were conducted using intracellular neutron autoradiography, providing valuable insights into the spatial distribution of the nanoparticles within cells. These findings strongly indicate that the developed nanomaterials hold significant promise as effective carriers for 10B in BNCT, showcasing their potential for advancing cancer treatment methodologies.
publishDate 2024
dc.date.none.fl_str_mv 2024-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/236637
Demichelis, Maria Paola; Portu, Agustina Mariana; Gadan, Mario Alberto; Vitali, Agostina; Forlingieri, Valentina; et al.; Synthesis and Characterization of B4C-Based Multifunctional Nanoparticles for Boron Neutron Capture Therapy Applications; MDPI; Applied Nano; 5; 2; 3-2024; 33-47
2673-3501
CONICET Digital
CONICET
url http://hdl.handle.net/11336/236637
identifier_str_mv Demichelis, Maria Paola; Portu, Agustina Mariana; Gadan, Mario Alberto; Vitali, Agostina; Forlingieri, Valentina; et al.; Synthesis and Characterization of B4C-Based Multifunctional Nanoparticles for Boron Neutron Capture Therapy Applications; MDPI; Applied Nano; 5; 2; 3-2024; 33-47
2673-3501
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2673-3501/5/2/4
info:eu-repo/semantics/altIdentifier/doi/10.3390/applnano5020004
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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