Comparison of complement system activity amongst wild and domestic animals

Autores
Moleón Bersani, María Soledad; Merchant, Mark E.; Ortega, Hugo Hector; Siroski, Pablo Ariel
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Multiple mechanisms have evolved for the defensive recognition of foreign components, such as microor-ganisms. The majority of immunological studies with vertebrates have been focused on endothermic species, and relatively little attention has been directed toward ectothermic vertebrates. We employed a colorimetric assay designed to compare plasma hemolytic activities based on the serum complement system (CS) activities amongst some representative reptiles, wild and domestic birds, and mammals. Results obtained from the hemolytic assays conducted with plasma derived from all of the animal species used showed that broad-snouted caiman had the highest activity, and no differences were observed in the hemolytic activities of plasma from birds or the other reptile species. In contrast, the CS activity obtained with mammalian plasma was markedly lower than that from the other taxa. This assay has many advantages, such as the requirement of small sample volume, reproducible results, and low cost. In addition, unsensitized sheep red blood cell hemolysis can be successfully used for the evaluation of innate immune system activities in non-mammalian species; however, for mammals, it should be combined with other immunological determinates to evaluate integral innate immunocompetence.
Fil: Moleón Bersani, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Merchant, Mark E.. Mcneese State University,; Estados Unidos
Fil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Siroski, Pablo Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Materia
COMPLEMENT SYSTEM
DOMESTIC ANIMALS
IMMUNOCOMPETENCE
INNATE IMMUNITY
WILDLIFE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/115826
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Comparison of complement system activity amongst wild and domestic animalsMoleón Bersani, María SoledadMerchant, Mark E.Ortega, Hugo HectorSiroski, Pablo ArielCOMPLEMENT SYSTEMDOMESTIC ANIMALSIMMUNOCOMPETENCEINNATE IMMUNITYWILDLIFEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Multiple mechanisms have evolved for the defensive recognition of foreign components, such as microor-ganisms. The majority of immunological studies with vertebrates have been focused on endothermic species, and relatively little attention has been directed toward ectothermic vertebrates. We employed a colorimetric assay designed to compare plasma hemolytic activities based on the serum complement system (CS) activities amongst some representative reptiles, wild and domestic birds, and mammals. Results obtained from the hemolytic assays conducted with plasma derived from all of the animal species used showed that broad-snouted caiman had the highest activity, and no differences were observed in the hemolytic activities of plasma from birds or the other reptile species. In contrast, the CS activity obtained with mammalian plasma was markedly lower than that from the other taxa. This assay has many advantages, such as the requirement of small sample volume, reproducible results, and low cost. In addition, unsensitized sheep red blood cell hemolysis can be successfully used for the evaluation of innate immune system activities in non-mammalian species; however, for mammals, it should be combined with other immunological determinates to evaluate integral innate immunocompetence.Fil: Moleón Bersani, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Merchant, Mark E.. Mcneese State University,; Estados UnidosFil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Siroski, Pablo Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFirenze University Press2020-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/115826Moleón Bersani, María Soledad; Merchant, Mark E.; Ortega, Hugo Hector; Siroski, Pablo Ariel; Comparison of complement system activity amongst wild and domestic animals; Firenze University Press; Acta Herpetologica; 15; 1; 5-2020; 59-641827-96351827-9643CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://oaj.fupress.net/index.php/ah/article/view/7903info:eu-repo/semantics/altIdentifier/doi/10.13128/a_h-7903info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:32:15Zoai:ri.conicet.gov.ar:11336/115826instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:32:15.647CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Comparison of complement system activity amongst wild and domestic animals
title Comparison of complement system activity amongst wild and domestic animals
spellingShingle Comparison of complement system activity amongst wild and domestic animals
Moleón Bersani, María Soledad
COMPLEMENT SYSTEM
DOMESTIC ANIMALS
IMMUNOCOMPETENCE
INNATE IMMUNITY
WILDLIFE
title_short Comparison of complement system activity amongst wild and domestic animals
title_full Comparison of complement system activity amongst wild and domestic animals
title_fullStr Comparison of complement system activity amongst wild and domestic animals
title_full_unstemmed Comparison of complement system activity amongst wild and domestic animals
title_sort Comparison of complement system activity amongst wild and domestic animals
dc.creator.none.fl_str_mv Moleón Bersani, María Soledad
Merchant, Mark E.
Ortega, Hugo Hector
Siroski, Pablo Ariel
author Moleón Bersani, María Soledad
author_facet Moleón Bersani, María Soledad
Merchant, Mark E.
Ortega, Hugo Hector
Siroski, Pablo Ariel
author_role author
author2 Merchant, Mark E.
Ortega, Hugo Hector
Siroski, Pablo Ariel
author2_role author
author
author
dc.subject.none.fl_str_mv COMPLEMENT SYSTEM
DOMESTIC ANIMALS
IMMUNOCOMPETENCE
INNATE IMMUNITY
WILDLIFE
topic COMPLEMENT SYSTEM
DOMESTIC ANIMALS
IMMUNOCOMPETENCE
INNATE IMMUNITY
WILDLIFE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Multiple mechanisms have evolved for the defensive recognition of foreign components, such as microor-ganisms. The majority of immunological studies with vertebrates have been focused on endothermic species, and relatively little attention has been directed toward ectothermic vertebrates. We employed a colorimetric assay designed to compare plasma hemolytic activities based on the serum complement system (CS) activities amongst some representative reptiles, wild and domestic birds, and mammals. Results obtained from the hemolytic assays conducted with plasma derived from all of the animal species used showed that broad-snouted caiman had the highest activity, and no differences were observed in the hemolytic activities of plasma from birds or the other reptile species. In contrast, the CS activity obtained with mammalian plasma was markedly lower than that from the other taxa. This assay has many advantages, such as the requirement of small sample volume, reproducible results, and low cost. In addition, unsensitized sheep red blood cell hemolysis can be successfully used for the evaluation of innate immune system activities in non-mammalian species; however, for mammals, it should be combined with other immunological determinates to evaluate integral innate immunocompetence.
Fil: Moleón Bersani, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Merchant, Mark E.. Mcneese State University,; Estados Unidos
Fil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Siroski, Pablo Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
description Multiple mechanisms have evolved for the defensive recognition of foreign components, such as microor-ganisms. The majority of immunological studies with vertebrates have been focused on endothermic species, and relatively little attention has been directed toward ectothermic vertebrates. We employed a colorimetric assay designed to compare plasma hemolytic activities based on the serum complement system (CS) activities amongst some representative reptiles, wild and domestic birds, and mammals. Results obtained from the hemolytic assays conducted with plasma derived from all of the animal species used showed that broad-snouted caiman had the highest activity, and no differences were observed in the hemolytic activities of plasma from birds or the other reptile species. In contrast, the CS activity obtained with mammalian plasma was markedly lower than that from the other taxa. This assay has many advantages, such as the requirement of small sample volume, reproducible results, and low cost. In addition, unsensitized sheep red blood cell hemolysis can be successfully used for the evaluation of innate immune system activities in non-mammalian species; however, for mammals, it should be combined with other immunological determinates to evaluate integral innate immunocompetence.
publishDate 2020
dc.date.none.fl_str_mv 2020-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/115826
Moleón Bersani, María Soledad; Merchant, Mark E.; Ortega, Hugo Hector; Siroski, Pablo Ariel; Comparison of complement system activity amongst wild and domestic animals; Firenze University Press; Acta Herpetologica; 15; 1; 5-2020; 59-64
1827-9635
1827-9643
CONICET Digital
CONICET
url http://hdl.handle.net/11336/115826
identifier_str_mv Moleón Bersani, María Soledad; Merchant, Mark E.; Ortega, Hugo Hector; Siroski, Pablo Ariel; Comparison of complement system activity amongst wild and domestic animals; Firenze University Press; Acta Herpetologica; 15; 1; 5-2020; 59-64
1827-9635
1827-9643
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://oaj.fupress.net/index.php/ah/article/view/7903
info:eu-repo/semantics/altIdentifier/doi/10.13128/a_h-7903
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Firenze University Press
publisher.none.fl_str_mv Firenze University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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