A simple method to evaluate the number of bradyrhizobia on soybean seeds and its implication on inoculant quality control

Autores
Penna, Claudio; Massa, Rosana; Olivieri, Florencia Pia; Gutkind, Gabriel Osvaldo; Cassan, Fabricio Dario
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Soybean seeds are non-sterile and their bacterial population interferes with the enumeration of beneficial bacteria, making it difficult to assess survival under different conditions. Within this context, the principal aims of this work were: (1) to improve a selective media for the enumeration of B. japonicum recovered from inoculated soybean seeds; (2) to establish the most representative mathematical function for B. japonicum mortality on soybean seeds after inoculation; (3) to evaluate if environmental or physiological conditions modify B. japonicum mortality on soybean seeds; and (4) to create a new protocol for quality control of soybean inoculants. We successfully evaluated the combination of pentachloronitrobenzene and vancomycin added to the yeast-mannitol medium to inhibit most fungi and Gram-positive soybean microbiota, thus producing reliable counts of B. japonicum from inoculated soybean seeds. Percentages of recovery and survival factors were obtained and used to construct a two-phase exponential decay non-linear regression function. High temperature and desiccation decreased these parameters, while the optimization of temperature and the use of osmoprotective compounds with inoculants increased them. The use of this protocol minimized heterogeneity between experiments and may be considered more reliable than the simple expression of direct colony count of bacteria recovered from seeds.
Fil: Penna, Claudio. Merck Crop Bioscience Argentina. Laboratorio de Investigación y Desarrollo Básico; Argentina
Fil: Massa, Rosana. Merck Crop Bioscience Argentina. Laboratorio de Investigación y Desarrollo Básico; Argentina
Fil: Olivieri, Florencia Pia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gutkind, Gabriel Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cassan, Fabricio Dario. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Agrobiotecnológicas; Argentina
Materia
BRADYRHIZOBIUM JAPONICUM
INOCULANTS
QUALITY CONTROL
SOYBEAN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/191874
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/191874
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A simple method to evaluate the number of bradyrhizobia on soybean seeds and its implication on inoculant quality controlPenna, ClaudioMassa, RosanaOlivieri, Florencia PiaGutkind, Gabriel OsvaldoCassan, Fabricio DarioBRADYRHIZOBIUM JAPONICUMINOCULANTSQUALITY CONTROLSOYBEANhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Soybean seeds are non-sterile and their bacterial population interferes with the enumeration of beneficial bacteria, making it difficult to assess survival under different conditions. Within this context, the principal aims of this work were: (1) to improve a selective media for the enumeration of B. japonicum recovered from inoculated soybean seeds; (2) to establish the most representative mathematical function for B. japonicum mortality on soybean seeds after inoculation; (3) to evaluate if environmental or physiological conditions modify B. japonicum mortality on soybean seeds; and (4) to create a new protocol for quality control of soybean inoculants. We successfully evaluated the combination of pentachloronitrobenzene and vancomycin added to the yeast-mannitol medium to inhibit most fungi and Gram-positive soybean microbiota, thus producing reliable counts of B. japonicum from inoculated soybean seeds. Percentages of recovery and survival factors were obtained and used to construct a two-phase exponential decay non-linear regression function. High temperature and desiccation decreased these parameters, while the optimization of temperature and the use of osmoprotective compounds with inoculants increased them. The use of this protocol minimized heterogeneity between experiments and may be considered more reliable than the simple expression of direct colony count of bacteria recovered from seeds.Fil: Penna, Claudio. Merck Crop Bioscience Argentina. Laboratorio de Investigación y Desarrollo Básico; ArgentinaFil: Massa, Rosana. Merck Crop Bioscience Argentina. Laboratorio de Investigación y Desarrollo Básico; ArgentinaFil: Olivieri, Florencia Pia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gutkind, Gabriel Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cassan, Fabricio Dario. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Agrobiotecnológicas; ArgentinaSpringer2011-07-19info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/191874Penna, Claudio; Massa, Rosana; Olivieri, Florencia Pia; Gutkind, Gabriel Osvaldo; Cassan, Fabricio Dario; A simple method to evaluate the number of bradyrhizobia on soybean seeds and its implication on inoculant quality control; Springer; AMB Express; 1; 21; 19-7-2011; 1-102191-0855CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1186%2F2191-0855-1-21info:eu-repo/semantics/altIdentifier/url/https://amb-express.springeropen.com/articles/10.1186/2191-0855-1-21info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:38:00Zoai:ri.conicet.gov.ar:11336/191874instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:38:01.079CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A simple method to evaluate the number of bradyrhizobia on soybean seeds and its implication on inoculant quality control
title A simple method to evaluate the number of bradyrhizobia on soybean seeds and its implication on inoculant quality control
spellingShingle A simple method to evaluate the number of bradyrhizobia on soybean seeds and its implication on inoculant quality control
Penna, Claudio
BRADYRHIZOBIUM JAPONICUM
INOCULANTS
QUALITY CONTROL
SOYBEAN
title_short A simple method to evaluate the number of bradyrhizobia on soybean seeds and its implication on inoculant quality control
title_full A simple method to evaluate the number of bradyrhizobia on soybean seeds and its implication on inoculant quality control
title_fullStr A simple method to evaluate the number of bradyrhizobia on soybean seeds and its implication on inoculant quality control
title_full_unstemmed A simple method to evaluate the number of bradyrhizobia on soybean seeds and its implication on inoculant quality control
title_sort A simple method to evaluate the number of bradyrhizobia on soybean seeds and its implication on inoculant quality control
dc.creator.none.fl_str_mv Penna, Claudio
Massa, Rosana
Olivieri, Florencia Pia
Gutkind, Gabriel Osvaldo
Cassan, Fabricio Dario
author Penna, Claudio
author_facet Penna, Claudio
Massa, Rosana
Olivieri, Florencia Pia
Gutkind, Gabriel Osvaldo
Cassan, Fabricio Dario
author_role author
author2 Massa, Rosana
Olivieri, Florencia Pia
Gutkind, Gabriel Osvaldo
Cassan, Fabricio Dario
author2_role author
author
author
author
dc.subject.none.fl_str_mv BRADYRHIZOBIUM JAPONICUM
INOCULANTS
QUALITY CONTROL
SOYBEAN
topic BRADYRHIZOBIUM JAPONICUM
INOCULANTS
QUALITY CONTROL
SOYBEAN
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Soybean seeds are non-sterile and their bacterial population interferes with the enumeration of beneficial bacteria, making it difficult to assess survival under different conditions. Within this context, the principal aims of this work were: (1) to improve a selective media for the enumeration of B. japonicum recovered from inoculated soybean seeds; (2) to establish the most representative mathematical function for B. japonicum mortality on soybean seeds after inoculation; (3) to evaluate if environmental or physiological conditions modify B. japonicum mortality on soybean seeds; and (4) to create a new protocol for quality control of soybean inoculants. We successfully evaluated the combination of pentachloronitrobenzene and vancomycin added to the yeast-mannitol medium to inhibit most fungi and Gram-positive soybean microbiota, thus producing reliable counts of B. japonicum from inoculated soybean seeds. Percentages of recovery and survival factors were obtained and used to construct a two-phase exponential decay non-linear regression function. High temperature and desiccation decreased these parameters, while the optimization of temperature and the use of osmoprotective compounds with inoculants increased them. The use of this protocol minimized heterogeneity between experiments and may be considered more reliable than the simple expression of direct colony count of bacteria recovered from seeds.
Fil: Penna, Claudio. Merck Crop Bioscience Argentina. Laboratorio de Investigación y Desarrollo Básico; Argentina
Fil: Massa, Rosana. Merck Crop Bioscience Argentina. Laboratorio de Investigación y Desarrollo Básico; Argentina
Fil: Olivieri, Florencia Pia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gutkind, Gabriel Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cassan, Fabricio Dario. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Agrobiotecnológicas; Argentina
description Soybean seeds are non-sterile and their bacterial population interferes with the enumeration of beneficial bacteria, making it difficult to assess survival under different conditions. Within this context, the principal aims of this work were: (1) to improve a selective media for the enumeration of B. japonicum recovered from inoculated soybean seeds; (2) to establish the most representative mathematical function for B. japonicum mortality on soybean seeds after inoculation; (3) to evaluate if environmental or physiological conditions modify B. japonicum mortality on soybean seeds; and (4) to create a new protocol for quality control of soybean inoculants. We successfully evaluated the combination of pentachloronitrobenzene and vancomycin added to the yeast-mannitol medium to inhibit most fungi and Gram-positive soybean microbiota, thus producing reliable counts of B. japonicum from inoculated soybean seeds. Percentages of recovery and survival factors were obtained and used to construct a two-phase exponential decay non-linear regression function. High temperature and desiccation decreased these parameters, while the optimization of temperature and the use of osmoprotective compounds with inoculants increased them. The use of this protocol minimized heterogeneity between experiments and may be considered more reliable than the simple expression of direct colony count of bacteria recovered from seeds.
publishDate 2011
dc.date.none.fl_str_mv 2011-07-19
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/191874
Penna, Claudio; Massa, Rosana; Olivieri, Florencia Pia; Gutkind, Gabriel Osvaldo; Cassan, Fabricio Dario; A simple method to evaluate the number of bradyrhizobia on soybean seeds and its implication on inoculant quality control; Springer; AMB Express; 1; 21; 19-7-2011; 1-10
2191-0855
CONICET Digital
CONICET
url http://hdl.handle.net/11336/191874
identifier_str_mv Penna, Claudio; Massa, Rosana; Olivieri, Florencia Pia; Gutkind, Gabriel Osvaldo; Cassan, Fabricio Dario; A simple method to evaluate the number of bradyrhizobia on soybean seeds and its implication on inoculant quality control; Springer; AMB Express; 1; 21; 19-7-2011; 1-10
2191-0855
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1186%2F2191-0855-1-21
info:eu-repo/semantics/altIdentifier/url/https://amb-express.springeropen.com/articles/10.1186/2191-0855-1-21
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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