Analysis of the denitrification pathway and greenhouse gases emissions in Bradyrhizobium sp. strains used as biofertilizers in South America

Autores
Obando Castellanos, Dolly Melissa; Correa-Galeote, David; Castellano-Hinojosa, Antonio; Gualpa, José Luis; Hidalgo, Alba; de Dios Alché, Juan; Bedmar, Eulogio; Cassan, Fabricio Dario
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aims Greenhouse gases are considered potential atmospheric pollutants, with agriculture being one of the main emission sources. The practice of inoculating soybean seeds with Bradyrhizobium sp. might contribute to nitrous oxide (N2O) emissions. We analyzed this capacity in five of the most used strains of Bradyrhizobium sp. in South America. Methods and Results We analyzed the denitrification pathway and N2O production by B. japonicum E109 and CPAC15, B. diazoefficiens CPAC7, and B. elkanii SEMIA 587 and SEMIA 5019, both in free‐living conditions and symbiosis with soybean. The in silico analysis indicated the absence of nosZ genes in B. japonicum and the presence of all denitrification genes in B. diazoefficiens strains, as well as the absence of nirK, norC and nosZ genes in B. elkanii. The in planta analysis confirmed the N2O production under saprophytic conditions or symbiosis with soybean roots nodules. In the last case up to 26·1 and 18·4 times higher in plants inoculated with SEMIA5019 and E109 respectively, than in those inoculated with USDA110. Conclusions The strains E109, SEMIA 5019, CPAC15 and SEMIA 587 showed the highest N2O production both as free‐living cells and in symbiotic conditions in comparison with USDA110 and CPAC7, which do have the nosZ gene. Although norC and nosZ could not be identified in silico or in vitro in SEMIA 587 and SEMIA 5019, these strains showed capacity to produce N2O in our experimental conditions. Significance and Impact of Study This is the first report to analyze and confirm the incomplete denitrification capacity and N2O production in four of the five most used strains of Bradyrhizobium sp. for soybean inoculation in South America.
Fil: Obando Castellanos, Dolly Melissa. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal y de la Interacción Planta-microorganismo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Correa-Galeote, David. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; España
Fil: Castellano-Hinojosa, Antonio. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; España
Fil: Gualpa, José Luis. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal y de la Interacción Planta-microorganismo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hidalgo, Alba. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; España
Fil: de Dios Alché, Juan. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; España
Fil: Bedmar, Eulogio. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; España
Fil: Cassan, Fabricio Dario. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Investigaciones Agrobiotecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Investigaciones Agrobiotecnologicas.; Argentina
Materia
Greenhouse gases
Denitrification
Bradyrhizobium
Inoculants
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/112320
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/112320
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Analysis of the denitrification pathway and greenhouse gases emissions in Bradyrhizobium sp. strains used as biofertilizers in South AmericaObando Castellanos, Dolly MelissaCorrea-Galeote, DavidCastellano-Hinojosa, AntonioGualpa, José LuisHidalgo, Albade Dios Alché, JuanBedmar, EulogioCassan, Fabricio DarioGreenhouse gasesDenitrificationBradyrhizobiumInoculantshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Aims Greenhouse gases are considered potential atmospheric pollutants, with agriculture being one of the main emission sources. The practice of inoculating soybean seeds with Bradyrhizobium sp. might contribute to nitrous oxide (N2O) emissions. We analyzed this capacity in five of the most used strains of Bradyrhizobium sp. in South America. Methods and Results We analyzed the denitrification pathway and N2O production by B. japonicum E109 and CPAC15, B. diazoefficiens CPAC7, and B. elkanii SEMIA 587 and SEMIA 5019, both in free‐living conditions and symbiosis with soybean. The in silico analysis indicated the absence of nosZ genes in B. japonicum and the presence of all denitrification genes in B. diazoefficiens strains, as well as the absence of nirK, norC and nosZ genes in B. elkanii. The in planta analysis confirmed the N2O production under saprophytic conditions or symbiosis with soybean roots nodules. In the last case up to 26·1 and 18·4 times higher in plants inoculated with SEMIA5019 and E109 respectively, than in those inoculated with USDA110. Conclusions The strains E109, SEMIA 5019, CPAC15 and SEMIA 587 showed the highest N2O production both as free‐living cells and in symbiotic conditions in comparison with USDA110 and CPAC7, which do have the nosZ gene. Although norC and nosZ could not be identified in silico or in vitro in SEMIA 587 and SEMIA 5019, these strains showed capacity to produce N2O in our experimental conditions. Significance and Impact of Study This is the first report to analyze and confirm the incomplete denitrification capacity and N2O production in four of the five most used strains of Bradyrhizobium sp. for soybean inoculation in South America.Fil: Obando Castellanos, Dolly Melissa. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal y de la Interacción Planta-microorganismo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Correa-Galeote, David. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; EspañaFil: Castellano-Hinojosa, Antonio. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; EspañaFil: Gualpa, José Luis. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal y de la Interacción Planta-microorganismo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Hidalgo, Alba. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; EspañaFil: de Dios Alché, Juan. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; EspañaFil: Bedmar, Eulogio. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; EspañaFil: Cassan, Fabricio Dario. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Investigaciones Agrobiotecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Investigaciones Agrobiotecnologicas.; ArgentinaWiley Blackwell Publishing, Inc2019-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/112320Obando Castellanos, Dolly Melissa; Correa-Galeote, David; Castellano-Hinojosa, Antonio; Gualpa, José Luis; Hidalgo, Alba; et al.; Analysis of the denitrification pathway and greenhouse gases emissions in Bradyrhizobium sp. strains used as biofertilizers in South America; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 127; 3; 6-2019; 739-7491364-50721365-2672CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://sfamjournals.onlinelibrary.wiley.com/doi/abs/10.1111/jam.14233info:eu-repo/semantics/altIdentifier/doi/10.1111/jam.14233info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:10Zoai:ri.conicet.gov.ar:11336/112320instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:10.322CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Analysis of the denitrification pathway and greenhouse gases emissions in Bradyrhizobium sp. strains used as biofertilizers in South America
title Analysis of the denitrification pathway and greenhouse gases emissions in Bradyrhizobium sp. strains used as biofertilizers in South America
spellingShingle Analysis of the denitrification pathway and greenhouse gases emissions in Bradyrhizobium sp. strains used as biofertilizers in South America
Obando Castellanos, Dolly Melissa
Greenhouse gases
Denitrification
Bradyrhizobium
Inoculants
title_short Analysis of the denitrification pathway and greenhouse gases emissions in Bradyrhizobium sp. strains used as biofertilizers in South America
title_full Analysis of the denitrification pathway and greenhouse gases emissions in Bradyrhizobium sp. strains used as biofertilizers in South America
title_fullStr Analysis of the denitrification pathway and greenhouse gases emissions in Bradyrhizobium sp. strains used as biofertilizers in South America
title_full_unstemmed Analysis of the denitrification pathway and greenhouse gases emissions in Bradyrhizobium sp. strains used as biofertilizers in South America
title_sort Analysis of the denitrification pathway and greenhouse gases emissions in Bradyrhizobium sp. strains used as biofertilizers in South America
dc.creator.none.fl_str_mv Obando Castellanos, Dolly Melissa
Correa-Galeote, David
Castellano-Hinojosa, Antonio
Gualpa, José Luis
Hidalgo, Alba
de Dios Alché, Juan
Bedmar, Eulogio
Cassan, Fabricio Dario
author Obando Castellanos, Dolly Melissa
author_facet Obando Castellanos, Dolly Melissa
Correa-Galeote, David
Castellano-Hinojosa, Antonio
Gualpa, José Luis
Hidalgo, Alba
de Dios Alché, Juan
Bedmar, Eulogio
Cassan, Fabricio Dario
author_role author
author2 Correa-Galeote, David
Castellano-Hinojosa, Antonio
Gualpa, José Luis
Hidalgo, Alba
de Dios Alché, Juan
Bedmar, Eulogio
Cassan, Fabricio Dario
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Greenhouse gases
Denitrification
Bradyrhizobium
Inoculants
topic Greenhouse gases
Denitrification
Bradyrhizobium
Inoculants
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Aims Greenhouse gases are considered potential atmospheric pollutants, with agriculture being one of the main emission sources. The practice of inoculating soybean seeds with Bradyrhizobium sp. might contribute to nitrous oxide (N2O) emissions. We analyzed this capacity in five of the most used strains of Bradyrhizobium sp. in South America. Methods and Results We analyzed the denitrification pathway and N2O production by B. japonicum E109 and CPAC15, B. diazoefficiens CPAC7, and B. elkanii SEMIA 587 and SEMIA 5019, both in free‐living conditions and symbiosis with soybean. The in silico analysis indicated the absence of nosZ genes in B. japonicum and the presence of all denitrification genes in B. diazoefficiens strains, as well as the absence of nirK, norC and nosZ genes in B. elkanii. The in planta analysis confirmed the N2O production under saprophytic conditions or symbiosis with soybean roots nodules. In the last case up to 26·1 and 18·4 times higher in plants inoculated with SEMIA5019 and E109 respectively, than in those inoculated with USDA110. Conclusions The strains E109, SEMIA 5019, CPAC15 and SEMIA 587 showed the highest N2O production both as free‐living cells and in symbiotic conditions in comparison with USDA110 and CPAC7, which do have the nosZ gene. Although norC and nosZ could not be identified in silico or in vitro in SEMIA 587 and SEMIA 5019, these strains showed capacity to produce N2O in our experimental conditions. Significance and Impact of Study This is the first report to analyze and confirm the incomplete denitrification capacity and N2O production in four of the five most used strains of Bradyrhizobium sp. for soybean inoculation in South America.
Fil: Obando Castellanos, Dolly Melissa. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal y de la Interacción Planta-microorganismo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Correa-Galeote, David. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; España
Fil: Castellano-Hinojosa, Antonio. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; España
Fil: Gualpa, José Luis. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal y de la Interacción Planta-microorganismo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hidalgo, Alba. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; España
Fil: de Dios Alché, Juan. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; España
Fil: Bedmar, Eulogio. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; España
Fil: Cassan, Fabricio Dario. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Investigaciones Agrobiotecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Investigaciones Agrobiotecnologicas.; Argentina
description Aims Greenhouse gases are considered potential atmospheric pollutants, with agriculture being one of the main emission sources. The practice of inoculating soybean seeds with Bradyrhizobium sp. might contribute to nitrous oxide (N2O) emissions. We analyzed this capacity in five of the most used strains of Bradyrhizobium sp. in South America. Methods and Results We analyzed the denitrification pathway and N2O production by B. japonicum E109 and CPAC15, B. diazoefficiens CPAC7, and B. elkanii SEMIA 587 and SEMIA 5019, both in free‐living conditions and symbiosis with soybean. The in silico analysis indicated the absence of nosZ genes in B. japonicum and the presence of all denitrification genes in B. diazoefficiens strains, as well as the absence of nirK, norC and nosZ genes in B. elkanii. The in planta analysis confirmed the N2O production under saprophytic conditions or symbiosis with soybean roots nodules. In the last case up to 26·1 and 18·4 times higher in plants inoculated with SEMIA5019 and E109 respectively, than in those inoculated with USDA110. Conclusions The strains E109, SEMIA 5019, CPAC15 and SEMIA 587 showed the highest N2O production both as free‐living cells and in symbiotic conditions in comparison with USDA110 and CPAC7, which do have the nosZ gene. Although norC and nosZ could not be identified in silico or in vitro in SEMIA 587 and SEMIA 5019, these strains showed capacity to produce N2O in our experimental conditions. Significance and Impact of Study This is the first report to analyze and confirm the incomplete denitrification capacity and N2O production in four of the five most used strains of Bradyrhizobium sp. for soybean inoculation in South America.
publishDate 2019
dc.date.none.fl_str_mv 2019-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/112320
Obando Castellanos, Dolly Melissa; Correa-Galeote, David; Castellano-Hinojosa, Antonio; Gualpa, José Luis; Hidalgo, Alba; et al.; Analysis of the denitrification pathway and greenhouse gases emissions in Bradyrhizobium sp. strains used as biofertilizers in South America; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 127; 3; 6-2019; 739-749
1364-5072
1365-2672
CONICET Digital
CONICET
url http://hdl.handle.net/11336/112320
identifier_str_mv Obando Castellanos, Dolly Melissa; Correa-Galeote, David; Castellano-Hinojosa, Antonio; Gualpa, José Luis; Hidalgo, Alba; et al.; Analysis of the denitrification pathway and greenhouse gases emissions in Bradyrhizobium sp. strains used as biofertilizers in South America; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 127; 3; 6-2019; 739-749
1364-5072
1365-2672
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://sfamjournals.onlinelibrary.wiley.com/doi/abs/10.1111/jam.14233
info:eu-repo/semantics/altIdentifier/doi/10.1111/jam.14233
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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