Combination of organic acids and low-dose gamma irradiation as antimicrobial treatment to inactivate shiga toxin-producing escherichia coli inoculated in beef trimmings: Lack of be...

Autores: Cap, Mariana; Cingolani, Celeste; Lires, Carla; Mozgovoj, Marina Valeria; Soteras, Trinidad; Sucari, Adriana; Gentiluomo, Jimena; Descalzo, Adriana Maria; Grigioni, Gabriela Maria; Signorini Porchietto, Marcelo Lisandro; Horak, Celina Ines; Vaudagna, Sergio Ramon; Leotta, Gerardo Anibal
Año de publicación: 2020
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: The aim of this study was to assess the efficacy of lactic acid (LA), caprylic acid (CA), high-(HDI) and low- (LDI) dose gamma irradiation and LDI combined with LA or CA on the inactivation of a pool of Shiga toxin-producing Escherichia coli (STEC) strains inoculated on beef trimmings. The three most efficacious treatments were selected to study their effect on meat quality parameters and sensory attributes. The inoculum included five native STEC serogroups (O26, O103, O111, O145 and O157). The treatments applied were 0.5% LA, 0.04% CA, 0.5 kGy LDI, 2 kGy HDI, LDI+LA and LDI+CA. Beef trimmings were divided into two groups; one was inoculated with high (7 log CFU/g) and the other with low (1 log CFU/g) level of inoculum. Efficacy was assessed by estimating log reduction and reduction of stx- and eae-positive samples after enrichment, respectively. Results showed that treatments with organic acids alone were not effective in reducing STEC populations. For high inoculum samples, the most effective treatment was HDI followed by LDI+LA and LDI alone or combined with CA. For low inoculum samples, the most effective treatment was HDI followed by LDI alone or combined with organic acids. Concerning meat quality parameters and sensory attributes, irradiation treatments (LDI and HDI) caused minimal changes, while LDI+LA modified them significantly compared with the control. Therefore, based on our results, no benefits were observed after combining organic acids with gamma irradiation.
Fil: Cap, Mariana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Agroindustria. Instituto de Tecnología de Alimentos; Argentina
Fil: Cingolani, Celeste. Comisión Nacional de Energía Atómica. Centro Atómico Ezeiza; Argentina
Fil: Lires, Carla. Comisión Nacional de Energía Atómica. Centro Atómico Ezeiza; Argentina
Fil: Mozgovoj, Marina Valeria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Agroindustria. Instituto de Tecnología de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Soteras, Trinidad. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Agroindustria. Instituto de Tecnología de Alimentos; Argentina
Fil: Sucari, Adriana. Laboratorio de Alimentos Stamboulian. División Higiene y Seguridad Alimentaria y Ambiental; Argentina
Fil: Gentiluomo, Jimena. Laboratorio de Alimentos Stamboulian. División Higiene y Seguridad Alimentaria y Ambiental; Argentina
Fil: Descalzo, Adriana Maria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Agroindustria. Instituto de Tecnología de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Grigioni, Gabriela Maria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Agroindustria. Instituto de Tecnología de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Signorini Porchietto, Marcelo Lisandro. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; Argentina
Fil: Horak, Celina Ines. Comisión Nacional de Energía Atómica. Centro Atómico Ezeiza; Argentina
Fil: Vaudagna, Sergio Ramon. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Agroindustria. Instituto de Tecnología de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Leotta, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Materia: STEC
ORGANIC ACIDS
GAMMA IRRADIATION
MEAT
Nivel de accesibilidad: acceso abierto
Condiciones de uso: https://creativecommons.org/publicdomain/zero/1.0/
Repositorio
Institución: Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador: oai:ri.conicet.gov.ar:11336/139431

Acceder

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repository_id_str	3498
network_name_str	CONICET Digital (CONICET)
spelling	Combination of organic acids and low-dose gamma irradiation as antimicrobial treatment to inactivate shiga toxin-producing escherichia coli inoculated in beef trimmings: Lack of benefits in relation to single treatmentsCap, MarianaCingolani, CelesteLires, CarlaMozgovoj, Marina ValeriaSoteras, TrinidadSucari, AdrianaGentiluomo, JimenaDescalzo, Adriana MariaGrigioni, Gabriela MariaSignorini Porchietto, Marcelo LisandroHorak, Celina InesVaudagna, Sergio RamonLeotta, Gerardo AnibalSTECORGANIC ACIDSGAMMA IRRADIATIONMEAThttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2The aim of this study was to assess the efficacy of lactic acid (LA), caprylic acid (CA), high-(HDI) and low- (LDI) dose gamma irradiation and LDI combined with LA or CA on the inactivation of a pool of Shiga toxin-producing Escherichia coli (STEC) strains inoculated on beef trimmings. The three most efficacious treatments were selected to study their effect on meat quality parameters and sensory attributes. The inoculum included five native STEC serogroups (O26, O103, O111, O145 and O157). The treatments applied were 0.5% LA, 0.04% CA, 0.5 kGy LDI, 2 kGy HDI, LDI+LA and LDI+CA. Beef trimmings were divided into two groups; one was inoculated with high (7 log CFU/g) and the other with low (1 log CFU/g) level of inoculum. Efficacy was assessed by estimating log reduction and reduction of stx- and eae-positive samples after enrichment, respectively. Results showed that treatments with organic acids alone were not effective in reducing STEC populations. For high inoculum samples, the most effective treatment was HDI followed by LDI+LA and LDI alone or combined with CA. For low inoculum samples, the most effective treatment was HDI followed by LDI alone or combined with organic acids. Concerning meat quality parameters and sensory attributes, irradiation treatments (LDI and HDI) caused minimal changes, while LDI+LA modified them significantly compared with the control. Therefore, based on our results, no benefits were observed after combining organic acids with gamma irradiation.Fil: Cap, Mariana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Agroindustria. Instituto de Tecnología de Alimentos; ArgentinaFil: Cingolani, Celeste. Comisión Nacional de Energía Atómica. Centro Atómico Ezeiza; ArgentinaFil: Lires, Carla. Comisión Nacional de Energía Atómica. Centro Atómico Ezeiza; ArgentinaFil: Mozgovoj, Marina Valeria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Agroindustria. Instituto de Tecnología de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Soteras, Trinidad. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Agroindustria. Instituto de Tecnología de Alimentos; ArgentinaFil: Sucari, Adriana. Laboratorio de Alimentos Stamboulian. División Higiene y Seguridad Alimentaria y Ambiental; ArgentinaFil: Gentiluomo, Jimena. Laboratorio de Alimentos Stamboulian. División Higiene y Seguridad Alimentaria y Ambiental; ArgentinaFil: Descalzo, Adriana Maria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Agroindustria. Instituto de Tecnología de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Grigioni, Gabriela Maria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Agroindustria. Instituto de Tecnología de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Signorini Porchietto, Marcelo Lisandro. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; ArgentinaFil: Horak, Celina Ines. Comisión Nacional de Energía Atómica. Centro Atómico Ezeiza; ArgentinaFil: Vaudagna, Sergio Ramon. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Agroindustria. Instituto de Tecnología de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Leotta, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaPublic Library of Science2020-03-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/139431Cap, Mariana; Cingolani, Celeste; Lires, Carla; Mozgovoj, Marina Valeria; Soteras, Trinidad; et al.; Combination of organic acids and low-dose gamma irradiation as antimicrobial treatment to inactivate shiga toxin-producing escherichia coli inoculated in beef trimmings: Lack of benefits in relation to single treatments; Public Library of Science; Plos One; 15; 3; 26-3-2020; 1-131932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0230812info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0230812info:eu-repo/semantics/openAccesshttps://creativecommons.org/publicdomain/zero/1.0/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:50:14Zoai:ri.conicet.gov.ar:11336/139431instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:50:14.78CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	Combination of organic acids and low-dose gamma irradiation as antimicrobial treatment to inactivate shiga toxin-producing escherichia coli inoculated in beef trimmings: Lack of benefits in relation to single treatments
title	Combination of organic acids and low-dose gamma irradiation as antimicrobial treatment to inactivate shiga toxin-producing escherichia coli inoculated in beef trimmings: Lack of benefits in relation to single treatments
spellingShingle	Combination of organic acids and low-dose gamma irradiation as antimicrobial treatment to inactivate shiga toxin-producing escherichia coli inoculated in beef trimmings: Lack of benefits in relation to single treatments Cap, Mariana STEC ORGANIC ACIDS GAMMA IRRADIATION MEAT
title_short	Combination of organic acids and low-dose gamma irradiation as antimicrobial treatment to inactivate shiga toxin-producing escherichia coli inoculated in beef trimmings: Lack of benefits in relation to single treatments
title_full	Combination of organic acids and low-dose gamma irradiation as antimicrobial treatment to inactivate shiga toxin-producing escherichia coli inoculated in beef trimmings: Lack of benefits in relation to single treatments
title_fullStr	Combination of organic acids and low-dose gamma irradiation as antimicrobial treatment to inactivate shiga toxin-producing escherichia coli inoculated in beef trimmings: Lack of benefits in relation to single treatments
title_full_unstemmed	Combination of organic acids and low-dose gamma irradiation as antimicrobial treatment to inactivate shiga toxin-producing escherichia coli inoculated in beef trimmings: Lack of benefits in relation to single treatments
title_sort	Combination of organic acids and low-dose gamma irradiation as antimicrobial treatment to inactivate shiga toxin-producing escherichia coli inoculated in beef trimmings: Lack of benefits in relation to single treatments
dc.creator.none.fl_str_mv	Cap, Mariana Cingolani, Celeste Lires, Carla Mozgovoj, Marina Valeria Soteras, Trinidad Sucari, Adriana Gentiluomo, Jimena Descalzo, Adriana Maria Grigioni, Gabriela Maria Signorini Porchietto, Marcelo Lisandro Horak, Celina Ines Vaudagna, Sergio Ramon Leotta, Gerardo Anibal
author	Cap, Mariana
author_facet	Cap, Mariana Cingolani, Celeste Lires, Carla Mozgovoj, Marina Valeria Soteras, Trinidad Sucari, Adriana Gentiluomo, Jimena Descalzo, Adriana Maria Grigioni, Gabriela Maria Signorini Porchietto, Marcelo Lisandro Horak, Celina Ines Vaudagna, Sergio Ramon Leotta, Gerardo Anibal
author_role	author
author2	Cingolani, Celeste Lires, Carla Mozgovoj, Marina Valeria Soteras, Trinidad Sucari, Adriana Gentiluomo, Jimena Descalzo, Adriana Maria Grigioni, Gabriela Maria Signorini Porchietto, Marcelo Lisandro Horak, Celina Ines Vaudagna, Sergio Ramon Leotta, Gerardo Anibal
author2_role	author author author author author author author author author author author author
dc.subject.none.fl_str_mv	STEC ORGANIC ACIDS GAMMA IRRADIATION MEAT
topic	STEC ORGANIC ACIDS GAMMA IRRADIATION MEAT
purl_subject.fl_str_mv	https://purl.org/becyt/ford/2.11 https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv	The aim of this study was to assess the efficacy of lactic acid (LA), caprylic acid (CA), high-(HDI) and low- (LDI) dose gamma irradiation and LDI combined with LA or CA on the inactivation of a pool of Shiga toxin-producing Escherichia coli (STEC) strains inoculated on beef trimmings. The three most efficacious treatments were selected to study their effect on meat quality parameters and sensory attributes. The inoculum included five native STEC serogroups (O26, O103, O111, O145 and O157). The treatments applied were 0.5% LA, 0.04% CA, 0.5 kGy LDI, 2 kGy HDI, LDI+LA and LDI+CA. Beef trimmings were divided into two groups; one was inoculated with high (7 log CFU/g) and the other with low (1 log CFU/g) level of inoculum. Efficacy was assessed by estimating log reduction and reduction of stx- and eae-positive samples after enrichment, respectively. Results showed that treatments with organic acids alone were not effective in reducing STEC populations. For high inoculum samples, the most effective treatment was HDI followed by LDI+LA and LDI alone or combined with CA. For low inoculum samples, the most effective treatment was HDI followed by LDI alone or combined with organic acids. Concerning meat quality parameters and sensory attributes, irradiation treatments (LDI and HDI) caused minimal changes, while LDI+LA modified them significantly compared with the control. Therefore, based on our results, no benefits were observed after combining organic acids with gamma irradiation. Fil: Cap, Mariana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Agroindustria. Instituto de Tecnología de Alimentos; Argentina Fil: Cingolani, Celeste. Comisión Nacional de Energía Atómica. Centro Atómico Ezeiza; Argentina Fil: Lires, Carla. Comisión Nacional de Energía Atómica. Centro Atómico Ezeiza; Argentina Fil: Mozgovoj, Marina Valeria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Agroindustria. Instituto de Tecnología de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Soteras, Trinidad. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Agroindustria. Instituto de Tecnología de Alimentos; Argentina Fil: Sucari, Adriana. Laboratorio de Alimentos Stamboulian. División Higiene y Seguridad Alimentaria y Ambiental; Argentina Fil: Gentiluomo, Jimena. Laboratorio de Alimentos Stamboulian. División Higiene y Seguridad Alimentaria y Ambiental; Argentina Fil: Descalzo, Adriana Maria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Agroindustria. Instituto de Tecnología de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Grigioni, Gabriela Maria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Agroindustria. Instituto de Tecnología de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Signorini Porchietto, Marcelo Lisandro. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; Argentina Fil: Horak, Celina Ines. Comisión Nacional de Energía Atómica. Centro Atómico Ezeiza; Argentina Fil: Vaudagna, Sergio Ramon. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Agroindustria. Instituto de Tecnología de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Leotta, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
description	The aim of this study was to assess the efficacy of lactic acid (LA), caprylic acid (CA), high-(HDI) and low- (LDI) dose gamma irradiation and LDI combined with LA or CA on the inactivation of a pool of Shiga toxin-producing Escherichia coli (STEC) strains inoculated on beef trimmings. The three most efficacious treatments were selected to study their effect on meat quality parameters and sensory attributes. The inoculum included five native STEC serogroups (O26, O103, O111, O145 and O157). The treatments applied were 0.5% LA, 0.04% CA, 0.5 kGy LDI, 2 kGy HDI, LDI+LA and LDI+CA. Beef trimmings were divided into two groups; one was inoculated with high (7 log CFU/g) and the other with low (1 log CFU/g) level of inoculum. Efficacy was assessed by estimating log reduction and reduction of stx- and eae-positive samples after enrichment, respectively. Results showed that treatments with organic acids alone were not effective in reducing STEC populations. For high inoculum samples, the most effective treatment was HDI followed by LDI+LA and LDI alone or combined with CA. For low inoculum samples, the most effective treatment was HDI followed by LDI alone or combined with organic acids. Concerning meat quality parameters and sensory attributes, irradiation treatments (LDI and HDI) caused minimal changes, while LDI+LA modified them significantly compared with the control. Therefore, based on our results, no benefits were observed after combining organic acids with gamma irradiation.
publishDate	2020
dc.date.none.fl_str_mv	2020-03-26
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/139431 Cap, Mariana; Cingolani, Celeste; Lires, Carla; Mozgovoj, Marina Valeria; Soteras, Trinidad; et al.; Combination of organic acids and low-dose gamma irradiation as antimicrobial treatment to inactivate shiga toxin-producing escherichia coli inoculated in beef trimmings: Lack of benefits in relation to single treatments; Public Library of Science; Plos One; 15; 3; 26-3-2020; 1-13 1932-6203 CONICET Digital CONICET
url	http://hdl.handle.net/11336/139431
identifier_str_mv	Cap, Mariana; Cingolani, Celeste; Lires, Carla; Mozgovoj, Marina Valeria; Soteras, Trinidad; et al.; Combination of organic acids and low-dose gamma irradiation as antimicrobial treatment to inactivate shiga toxin-producing escherichia coli inoculated in beef trimmings: Lack of benefits in relation to single treatments; Public Library of Science; Plos One; 15; 3; 26-3-2020; 1-13 1932-6203 CONICET Digital CONICET
dc.language.none.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0230812 info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0230812
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess https://creativecommons.org/publicdomain/zero/1.0/
eu_rights_str_mv	openAccess
rights_invalid_str_mv	https://creativecommons.org/publicdomain/zero/1.0/
dc.format.none.fl_str_mv	application/pdf application/pdf application/pdf
dc.publisher.none.fl_str_mv	Public Library of Science
publisher.none.fl_str_mv	Public Library of Science
dc.source.none.fl_str_mv	reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str	CONICET Digital (CONICET)
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instname_str	Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv	CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv	dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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Combination of organic acids and low-dose gamma irradiation as antimicrobial treatment to inactivate shiga toxin-producing escherichia coli inoculated in beef trimmings: Lack of be...

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