Combination of organic acids and low-dose gamma irradiation as antimicrobial treatment to inactivate Shiga toxin-producing Escherichia coli inoculated in beef trimmings: Lack of be...
- Autores
- Cap, Mariana; Cingolani, Celeste; Lires, Carla; Mozgovoj, Marina Valeria; Soteras, Trinidad; Sucari, Adriana; Gentiluomo, Jimena; Descalzo, Adriana Maria; Grigioni, Gabriela Maria; Signorini, Marcelo; Horak, Celina; Vaudagna, Sergio Ramon; Leotta, Gerardo
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The aim of this study was to assess the efficacy of lactic acid (LA), caprylic acid (CA), high- (HDI) and low- (LDI) dose gamma irradiation and LDI combined with LA or CA on the inactivation of a pool of Shiga toxin-producing Escherichia coli (STEC) strains inoculated on beef trimmings. The three most efficacious treatments were selected to study their effect on meat quality parameters and sensory attributes. The inoculum included five native STEC serogroups (O26, O103, O111, O145 and O157). The treatments applied were 0.5% LA, 0.04% CA, 0.5 kGy LDI, 2 kGy HDI, LDI+LA and LDI+CA. Beef trimmings were divided into two groups; one was inoculated with high (7 log CFU/g) and the other with low (1 log CFU/g) level of inoculum. Efficacy was assessed by estimating log reduction and reduction of stx- and eae-positive samples after enrichment, respectively. Results showed that treatments with organic acids alone were not effective in reducing STEC populations. For high inoculum samples, the most effective treatment was HDI followed by LDI+LA and LDI alone or combined with CA. For low inoculum samples, the most effective treatment was HDI followed by LDI alone or combined with organic acids. Concerning meat quality parameters and sensory attributes, irradiation treatments (LDI and HDI) caused minimal changes, while LDI+LA modified them significantly compared with the control. Therefore, based on our results, no benefits were observed after combining organic acids with gamma irradiation.
Fil: Cap, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto Tecnología de Alimentos; Argentina.
Fil: Cingolani, Celeste. Comisión Nacional de Energía Atómica (CNEA). Centro Atómico Ezeiza; Argentina.
Fil: Lires, Carla. Comisión Nacional de Energía Atómica (CNEA). Centro Atómico Ezeiza; Argentina.
Fil: Mozgovoj, Marina Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto Tecnología de Alimentos; Argentina.
Fil: Soteras, Trinidad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto Tecnología de Alimentos; Argentina.
Fil: Sucari, Adriana. Laboratorio de Alimentos Stamboulian. División Higiene y Seguridad Alimentaria y Ambiental; Argentina.
Fil: Gentiluomo, Jimena. Laboratorio de Alimentos Stamboulian. División Higiene y Seguridad Alimentaria y Ambiental; Argentina.
Fil: Descalzo, Adriana Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto Tecnología de Alimentos; Argentina.
Fil: Grigioni, Gabriela Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto Tecnología de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina. Universidad de Morón; Argentina.
Fil: Signorini, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Horak, Celina. Comisión Nacional de Energía Atómica (CNEA). Centro Atómico Ezeiza; Argentina.
Fil: Vaudagna, Sergio Ramon. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto Tecnología de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina. Universidad de Morón; Argentina.
Fil: Leotta, Gerardo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias UNLP, IGEVET — (UNLP-CONICET LA PLATA). Instituto de Genética Veterinaria “Ing. Fernando N. Dulout ”; Argentina. - Fuente
- Plos One March 27 (2020)
- Materia
-
Organic Acids
Gamma Irradiation
Lactic Acid
Ácidos Orgánicos
Irradiación Gamma
Escherichia coli
Ácido Láctico
AntimicrobialTtreatment
Shiga Toxin
Beef Trimmings
Caprylic Acid
Tratamiento Antimicrobiano
Toxina Shiga
Recortes de Carne
Ácido Caprílico - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/7020
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Combination of organic acids and low-dose gamma irradiation as antimicrobial treatment to inactivate Shiga toxin-producing Escherichia coli inoculated in beef trimmings: Lack of benefits in relation to single treatmentsCap, MarianaCingolani, CelesteLires, CarlaMozgovoj, Marina ValeriaSoteras, TrinidadSucari, AdrianaGentiluomo, JimenaDescalzo, Adriana MariaGrigioni, Gabriela MariaSignorini, MarceloHorak, CelinaVaudagna, Sergio RamonLeotta, GerardoOrganic AcidsGamma IrradiationLactic AcidÁcidos OrgánicosIrradiación GammaEscherichia coliÁcido LácticoAntimicrobialTtreatmentShiga ToxinBeef TrimmingsCaprylic AcidTratamiento AntimicrobianoToxina ShigaRecortes de CarneÁcido CaprílicoThe aim of this study was to assess the efficacy of lactic acid (LA), caprylic acid (CA), high- (HDI) and low- (LDI) dose gamma irradiation and LDI combined with LA or CA on the inactivation of a pool of Shiga toxin-producing Escherichia coli (STEC) strains inoculated on beef trimmings. The three most efficacious treatments were selected to study their effect on meat quality parameters and sensory attributes. The inoculum included five native STEC serogroups (O26, O103, O111, O145 and O157). The treatments applied were 0.5% LA, 0.04% CA, 0.5 kGy LDI, 2 kGy HDI, LDI+LA and LDI+CA. Beef trimmings were divided into two groups; one was inoculated with high (7 log CFU/g) and the other with low (1 log CFU/g) level of inoculum. Efficacy was assessed by estimating log reduction and reduction of stx- and eae-positive samples after enrichment, respectively. Results showed that treatments with organic acids alone were not effective in reducing STEC populations. For high inoculum samples, the most effective treatment was HDI followed by LDI+LA and LDI alone or combined with CA. For low inoculum samples, the most effective treatment was HDI followed by LDI alone or combined with organic acids. Concerning meat quality parameters and sensory attributes, irradiation treatments (LDI and HDI) caused minimal changes, while LDI+LA modified them significantly compared with the control. Therefore, based on our results, no benefits were observed after combining organic acids with gamma irradiation.Fil: Cap, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto Tecnología de Alimentos; Argentina.Fil: Cingolani, Celeste. Comisión Nacional de Energía Atómica (CNEA). Centro Atómico Ezeiza; Argentina.Fil: Lires, Carla. Comisión Nacional de Energía Atómica (CNEA). Centro Atómico Ezeiza; Argentina.Fil: Mozgovoj, Marina Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto Tecnología de Alimentos; Argentina.Fil: Soteras, Trinidad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto Tecnología de Alimentos; Argentina.Fil: Sucari, Adriana. Laboratorio de Alimentos Stamboulian. División Higiene y Seguridad Alimentaria y Ambiental; Argentina.Fil: Gentiluomo, Jimena. Laboratorio de Alimentos Stamboulian. División Higiene y Seguridad Alimentaria y Ambiental; Argentina.Fil: Descalzo, Adriana Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto Tecnología de Alimentos; Argentina.Fil: Grigioni, Gabriela Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto Tecnología de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina. Universidad de Morón; Argentina.Fil: Signorini, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Horak, Celina. Comisión Nacional de Energía Atómica (CNEA). Centro Atómico Ezeiza; Argentina.Fil: Vaudagna, Sergio Ramon. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto Tecnología de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina. Universidad de Morón; Argentina.Fil: Leotta, Gerardo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias UNLP, IGEVET — (UNLP-CONICET LA PLATA). Instituto de Genética Veterinaria “Ing. Fernando N. Dulout ”; Argentina.Plos ONE2020-04-02T12:09:04Z2020-04-02T12:09:04Z2020-03-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/7020https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0230812https://doi.org/10.1371/journal.pone.0230812Plos One March 27 (2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:44:55Zoai:localhost:20.500.12123/7020instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:55.319INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Combination of organic acids and low-dose gamma irradiation as antimicrobial treatment to inactivate Shiga toxin-producing Escherichia coli inoculated in beef trimmings: Lack of benefits in relation to single treatments |
title |
Combination of organic acids and low-dose gamma irradiation as antimicrobial treatment to inactivate Shiga toxin-producing Escherichia coli inoculated in beef trimmings: Lack of benefits in relation to single treatments |
spellingShingle |
Combination of organic acids and low-dose gamma irradiation as antimicrobial treatment to inactivate Shiga toxin-producing Escherichia coli inoculated in beef trimmings: Lack of benefits in relation to single treatments Cap, Mariana Organic Acids Gamma Irradiation Lactic Acid Ácidos Orgánicos Irradiación Gamma Escherichia coli Ácido Láctico AntimicrobialTtreatment Shiga Toxin Beef Trimmings Caprylic Acid Tratamiento Antimicrobiano Toxina Shiga Recortes de Carne Ácido Caprílico |
title_short |
Combination of organic acids and low-dose gamma irradiation as antimicrobial treatment to inactivate Shiga toxin-producing Escherichia coli inoculated in beef trimmings: Lack of benefits in relation to single treatments |
title_full |
Combination of organic acids and low-dose gamma irradiation as antimicrobial treatment to inactivate Shiga toxin-producing Escherichia coli inoculated in beef trimmings: Lack of benefits in relation to single treatments |
title_fullStr |
Combination of organic acids and low-dose gamma irradiation as antimicrobial treatment to inactivate Shiga toxin-producing Escherichia coli inoculated in beef trimmings: Lack of benefits in relation to single treatments |
title_full_unstemmed |
Combination of organic acids and low-dose gamma irradiation as antimicrobial treatment to inactivate Shiga toxin-producing Escherichia coli inoculated in beef trimmings: Lack of benefits in relation to single treatments |
title_sort |
Combination of organic acids and low-dose gamma irradiation as antimicrobial treatment to inactivate Shiga toxin-producing Escherichia coli inoculated in beef trimmings: Lack of benefits in relation to single treatments |
dc.creator.none.fl_str_mv |
Cap, Mariana Cingolani, Celeste Lires, Carla Mozgovoj, Marina Valeria Soteras, Trinidad Sucari, Adriana Gentiluomo, Jimena Descalzo, Adriana Maria Grigioni, Gabriela Maria Signorini, Marcelo Horak, Celina Vaudagna, Sergio Ramon Leotta, Gerardo |
author |
Cap, Mariana |
author_facet |
Cap, Mariana Cingolani, Celeste Lires, Carla Mozgovoj, Marina Valeria Soteras, Trinidad Sucari, Adriana Gentiluomo, Jimena Descalzo, Adriana Maria Grigioni, Gabriela Maria Signorini, Marcelo Horak, Celina Vaudagna, Sergio Ramon Leotta, Gerardo |
author_role |
author |
author2 |
Cingolani, Celeste Lires, Carla Mozgovoj, Marina Valeria Soteras, Trinidad Sucari, Adriana Gentiluomo, Jimena Descalzo, Adriana Maria Grigioni, Gabriela Maria Signorini, Marcelo Horak, Celina Vaudagna, Sergio Ramon Leotta, Gerardo |
author2_role |
author author author author author author author author author author author author |
dc.subject.none.fl_str_mv |
Organic Acids Gamma Irradiation Lactic Acid Ácidos Orgánicos Irradiación Gamma Escherichia coli Ácido Láctico AntimicrobialTtreatment Shiga Toxin Beef Trimmings Caprylic Acid Tratamiento Antimicrobiano Toxina Shiga Recortes de Carne Ácido Caprílico |
topic |
Organic Acids Gamma Irradiation Lactic Acid Ácidos Orgánicos Irradiación Gamma Escherichia coli Ácido Láctico AntimicrobialTtreatment Shiga Toxin Beef Trimmings Caprylic Acid Tratamiento Antimicrobiano Toxina Shiga Recortes de Carne Ácido Caprílico |
dc.description.none.fl_txt_mv |
The aim of this study was to assess the efficacy of lactic acid (LA), caprylic acid (CA), high- (HDI) and low- (LDI) dose gamma irradiation and LDI combined with LA or CA on the inactivation of a pool of Shiga toxin-producing Escherichia coli (STEC) strains inoculated on beef trimmings. The three most efficacious treatments were selected to study their effect on meat quality parameters and sensory attributes. The inoculum included five native STEC serogroups (O26, O103, O111, O145 and O157). The treatments applied were 0.5% LA, 0.04% CA, 0.5 kGy LDI, 2 kGy HDI, LDI+LA and LDI+CA. Beef trimmings were divided into two groups; one was inoculated with high (7 log CFU/g) and the other with low (1 log CFU/g) level of inoculum. Efficacy was assessed by estimating log reduction and reduction of stx- and eae-positive samples after enrichment, respectively. Results showed that treatments with organic acids alone were not effective in reducing STEC populations. For high inoculum samples, the most effective treatment was HDI followed by LDI+LA and LDI alone or combined with CA. For low inoculum samples, the most effective treatment was HDI followed by LDI alone or combined with organic acids. Concerning meat quality parameters and sensory attributes, irradiation treatments (LDI and HDI) caused minimal changes, while LDI+LA modified them significantly compared with the control. Therefore, based on our results, no benefits were observed after combining organic acids with gamma irradiation. Fil: Cap, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto Tecnología de Alimentos; Argentina. Fil: Cingolani, Celeste. Comisión Nacional de Energía Atómica (CNEA). Centro Atómico Ezeiza; Argentina. Fil: Lires, Carla. Comisión Nacional de Energía Atómica (CNEA). Centro Atómico Ezeiza; Argentina. Fil: Mozgovoj, Marina Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto Tecnología de Alimentos; Argentina. Fil: Soteras, Trinidad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto Tecnología de Alimentos; Argentina. Fil: Sucari, Adriana. Laboratorio de Alimentos Stamboulian. División Higiene y Seguridad Alimentaria y Ambiental; Argentina. Fil: Gentiluomo, Jimena. Laboratorio de Alimentos Stamboulian. División Higiene y Seguridad Alimentaria y Ambiental; Argentina. Fil: Descalzo, Adriana Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto Tecnología de Alimentos; Argentina. Fil: Grigioni, Gabriela Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto Tecnología de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina. Universidad de Morón; Argentina. Fil: Signorini, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Horak, Celina. Comisión Nacional de Energía Atómica (CNEA). Centro Atómico Ezeiza; Argentina. Fil: Vaudagna, Sergio Ramon. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto Tecnología de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina. Universidad de Morón; Argentina. Fil: Leotta, Gerardo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias UNLP, IGEVET — (UNLP-CONICET LA PLATA). Instituto de Genética Veterinaria “Ing. Fernando N. Dulout ”; Argentina. |
description |
The aim of this study was to assess the efficacy of lactic acid (LA), caprylic acid (CA), high- (HDI) and low- (LDI) dose gamma irradiation and LDI combined with LA or CA on the inactivation of a pool of Shiga toxin-producing Escherichia coli (STEC) strains inoculated on beef trimmings. The three most efficacious treatments were selected to study their effect on meat quality parameters and sensory attributes. The inoculum included five native STEC serogroups (O26, O103, O111, O145 and O157). The treatments applied were 0.5% LA, 0.04% CA, 0.5 kGy LDI, 2 kGy HDI, LDI+LA and LDI+CA. Beef trimmings were divided into two groups; one was inoculated with high (7 log CFU/g) and the other with low (1 log CFU/g) level of inoculum. Efficacy was assessed by estimating log reduction and reduction of stx- and eae-positive samples after enrichment, respectively. Results showed that treatments with organic acids alone were not effective in reducing STEC populations. For high inoculum samples, the most effective treatment was HDI followed by LDI+LA and LDI alone or combined with CA. For low inoculum samples, the most effective treatment was HDI followed by LDI alone or combined with organic acids. Concerning meat quality parameters and sensory attributes, irradiation treatments (LDI and HDI) caused minimal changes, while LDI+LA modified them significantly compared with the control. Therefore, based on our results, no benefits were observed after combining organic acids with gamma irradiation. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-04-02T12:09:04Z 2020-04-02T12:09:04Z 2020-03-26 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/7020 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0230812 https://doi.org/10.1371/journal.pone.0230812 |
url |
http://hdl.handle.net/20.500.12123/7020 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0230812 https://doi.org/10.1371/journal.pone.0230812 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Plos ONE |
publisher.none.fl_str_mv |
Plos ONE |
dc.source.none.fl_str_mv |
Plos One March 27 (2020) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
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INTA Digital (INTA) |
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Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
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tripaldi.nicolas@inta.gob.ar |
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