Evaluation of the chagas western blot igg assay for the diagnosis of chagas disease

Autores
Brossas, Jean Yves; Ballering, Griselda Edith; Bisio, Margarita María Catalina; Guihenneuc, Jeremy; Gulin, Julián Ernesto Nicolás; Jauréguiberry, S.; Lescure, François Xavier; Fekkar, Arnaud; Mazier, Dominique; Altcheh, Jaime Marcelo; Paris, Luc
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Chagas disease is a debilitating and often fatal pathology resulting from infection by the protozoan parasite Trypanosoma cruzi. In its recommendations, the World Health Organization states that the diagnosis of T. cruzi infection is usually based on the detection of antibodies against T. cruzi antigens and performed with two methodologically different assays. An inconclusive result can be resolved with a third “confirmatory” assay. The objective of this article is to evaluate the effectiveness of the Chagas Western Blot IgG assay (LDBio Diagnostics, Lyon, France) as a confirmatory serologic test. The Chagas Western Blot IgG assay was performed with native antigens derived from a T. cruzi strain of the TcVI genotype. Retrospective sera were provided by two parasitology laboratories (France and Argentina). The sensitivity, specificity, positive predictive value and negative predictive value of the Chagas blot were all 100% in our sera collection. The Chagas blot is an easy and qualitative method for the diagnosis of Chagas disease, with results in less than 2 h. This immunoblot has potential as a supplemental test for the confirmation of the presence of antibodies against T. cruzi in serum specimens. Nonetheless, the very good initial results presented here will need to be confirmed in larger studies.
Fil: Brossas, Jean Yves. Sorbonne Université Hôpital Pitié-Salpêtrière; Francia
Fil: Ballering, Griselda Edith. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; Argentina
Fil: Bisio, Margarita María Catalina. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; Argentina
Fil: Guihenneuc, Jeremy. Sorbonne Université Hôpital Pitié-Salpêtrière; Francia
Fil: Gulin, Julián Ernesto Nicolás. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Jauréguiberry, S.. Sorbonne Université Hôpital Pitié-Salpêtrière; Francia
Fil: Lescure, François Xavier. Hôpitaux Universitaires Paris Nord val de Seine; Francia
Fil: Fekkar, Arnaud. Sorbonne Université Hôpital Pitié-Salpêtrière; Francia
Fil: Mazier, Dominique. Sorbonne University; Francia
Fil: Altcheh, Jaime Marcelo. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; Argentina
Fil: Paris, Luc. Sorbonne Université Hôpital Pitié-Salpêtrière; Francia
Materia
CHAGAS DISEASE
DIAGNOSTIC
TRYPANOSOMA CRUZI
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/170558
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Evaluation of the chagas western blot igg assay for the diagnosis of chagas diseaseBrossas, Jean YvesBallering, Griselda EdithBisio, Margarita María CatalinaGuihenneuc, JeremyGulin, Julián Ernesto NicolásJauréguiberry, S.Lescure, François XavierFekkar, ArnaudMazier, DominiqueAltcheh, Jaime MarceloParis, LucCHAGAS DISEASEDIAGNOSTICTRYPANOSOMA CRUZIhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3https://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Chagas disease is a debilitating and often fatal pathology resulting from infection by the protozoan parasite Trypanosoma cruzi. In its recommendations, the World Health Organization states that the diagnosis of T. cruzi infection is usually based on the detection of antibodies against T. cruzi antigens and performed with two methodologically different assays. An inconclusive result can be resolved with a third “confirmatory” assay. The objective of this article is to evaluate the effectiveness of the Chagas Western Blot IgG assay (LDBio Diagnostics, Lyon, France) as a confirmatory serologic test. The Chagas Western Blot IgG assay was performed with native antigens derived from a T. cruzi strain of the TcVI genotype. Retrospective sera were provided by two parasitology laboratories (France and Argentina). The sensitivity, specificity, positive predictive value and negative predictive value of the Chagas blot were all 100% in our sera collection. The Chagas blot is an easy and qualitative method for the diagnosis of Chagas disease, with results in less than 2 h. This immunoblot has potential as a supplemental test for the confirmation of the presence of antibodies against T. cruzi in serum specimens. Nonetheless, the very good initial results presented here will need to be confirmed in larger studies.Fil: Brossas, Jean Yves. Sorbonne Université Hôpital Pitié-Salpêtrière; FranciaFil: Ballering, Griselda Edith. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; ArgentinaFil: Bisio, Margarita María Catalina. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; ArgentinaFil: Guihenneuc, Jeremy. Sorbonne Université Hôpital Pitié-Salpêtrière; FranciaFil: Gulin, Julián Ernesto Nicolás. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; ArgentinaFil: Jauréguiberry, S.. Sorbonne Université Hôpital Pitié-Salpêtrière; FranciaFil: Lescure, François Xavier. Hôpitaux Universitaires Paris Nord val de Seine; FranciaFil: Fekkar, Arnaud. Sorbonne Université Hôpital Pitié-Salpêtrière; FranciaFil: Mazier, Dominique. Sorbonne University; FranciaFil: Altcheh, Jaime Marcelo. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; ArgentinaFil: Paris, Luc. Sorbonne Université Hôpital Pitié-Salpêtrière; FranciaMDPI2021-11-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/170558Brossas, Jean Yves; Ballering, Griselda Edith; Bisio, Margarita María Catalina; Guihenneuc, Jeremy; Gulin, Julián Ernesto Nicolás; et al.; Evaluation of the chagas western blot igg assay for the diagnosis of chagas disease; MDPI; Pathogens; 10; 11; 10-11-2021; 1-102076-0817CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/pathogens10111455info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/22/5/2414info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T11:45:08Zoai:ri.conicet.gov.ar:11336/170558instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 11:45:08.424CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of the chagas western blot igg assay for the diagnosis of chagas disease
title Evaluation of the chagas western blot igg assay for the diagnosis of chagas disease
spellingShingle Evaluation of the chagas western blot igg assay for the diagnosis of chagas disease
Brossas, Jean Yves
CHAGAS DISEASE
DIAGNOSTIC
TRYPANOSOMA CRUZI
title_short Evaluation of the chagas western blot igg assay for the diagnosis of chagas disease
title_full Evaluation of the chagas western blot igg assay for the diagnosis of chagas disease
title_fullStr Evaluation of the chagas western blot igg assay for the diagnosis of chagas disease
title_full_unstemmed Evaluation of the chagas western blot igg assay for the diagnosis of chagas disease
title_sort Evaluation of the chagas western blot igg assay for the diagnosis of chagas disease
dc.creator.none.fl_str_mv Brossas, Jean Yves
Ballering, Griselda Edith
Bisio, Margarita María Catalina
Guihenneuc, Jeremy
Gulin, Julián Ernesto Nicolás
Jauréguiberry, S.
Lescure, François Xavier
Fekkar, Arnaud
Mazier, Dominique
Altcheh, Jaime Marcelo
Paris, Luc
author Brossas, Jean Yves
author_facet Brossas, Jean Yves
Ballering, Griselda Edith
Bisio, Margarita María Catalina
Guihenneuc, Jeremy
Gulin, Julián Ernesto Nicolás
Jauréguiberry, S.
Lescure, François Xavier
Fekkar, Arnaud
Mazier, Dominique
Altcheh, Jaime Marcelo
Paris, Luc
author_role author
author2 Ballering, Griselda Edith
Bisio, Margarita María Catalina
Guihenneuc, Jeremy
Gulin, Julián Ernesto Nicolás
Jauréguiberry, S.
Lescure, François Xavier
Fekkar, Arnaud
Mazier, Dominique
Altcheh, Jaime Marcelo
Paris, Luc
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CHAGAS DISEASE
DIAGNOSTIC
TRYPANOSOMA CRUZI
topic CHAGAS DISEASE
DIAGNOSTIC
TRYPANOSOMA CRUZI
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Chagas disease is a debilitating and often fatal pathology resulting from infection by the protozoan parasite Trypanosoma cruzi. In its recommendations, the World Health Organization states that the diagnosis of T. cruzi infection is usually based on the detection of antibodies against T. cruzi antigens and performed with two methodologically different assays. An inconclusive result can be resolved with a third “confirmatory” assay. The objective of this article is to evaluate the effectiveness of the Chagas Western Blot IgG assay (LDBio Diagnostics, Lyon, France) as a confirmatory serologic test. The Chagas Western Blot IgG assay was performed with native antigens derived from a T. cruzi strain of the TcVI genotype. Retrospective sera were provided by two parasitology laboratories (France and Argentina). The sensitivity, specificity, positive predictive value and negative predictive value of the Chagas blot were all 100% in our sera collection. The Chagas blot is an easy and qualitative method for the diagnosis of Chagas disease, with results in less than 2 h. This immunoblot has potential as a supplemental test for the confirmation of the presence of antibodies against T. cruzi in serum specimens. Nonetheless, the very good initial results presented here will need to be confirmed in larger studies.
Fil: Brossas, Jean Yves. Sorbonne Université Hôpital Pitié-Salpêtrière; Francia
Fil: Ballering, Griselda Edith. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; Argentina
Fil: Bisio, Margarita María Catalina. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; Argentina
Fil: Guihenneuc, Jeremy. Sorbonne Université Hôpital Pitié-Salpêtrière; Francia
Fil: Gulin, Julián Ernesto Nicolás. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Jauréguiberry, S.. Sorbonne Université Hôpital Pitié-Salpêtrière; Francia
Fil: Lescure, François Xavier. Hôpitaux Universitaires Paris Nord val de Seine; Francia
Fil: Fekkar, Arnaud. Sorbonne Université Hôpital Pitié-Salpêtrière; Francia
Fil: Mazier, Dominique. Sorbonne University; Francia
Fil: Altcheh, Jaime Marcelo. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; Argentina
Fil: Paris, Luc. Sorbonne Université Hôpital Pitié-Salpêtrière; Francia
description Chagas disease is a debilitating and often fatal pathology resulting from infection by the protozoan parasite Trypanosoma cruzi. In its recommendations, the World Health Organization states that the diagnosis of T. cruzi infection is usually based on the detection of antibodies against T. cruzi antigens and performed with two methodologically different assays. An inconclusive result can be resolved with a third “confirmatory” assay. The objective of this article is to evaluate the effectiveness of the Chagas Western Blot IgG assay (LDBio Diagnostics, Lyon, France) as a confirmatory serologic test. The Chagas Western Blot IgG assay was performed with native antigens derived from a T. cruzi strain of the TcVI genotype. Retrospective sera were provided by two parasitology laboratories (France and Argentina). The sensitivity, specificity, positive predictive value and negative predictive value of the Chagas blot were all 100% in our sera collection. The Chagas blot is an easy and qualitative method for the diagnosis of Chagas disease, with results in less than 2 h. This immunoblot has potential as a supplemental test for the confirmation of the presence of antibodies against T. cruzi in serum specimens. Nonetheless, the very good initial results presented here will need to be confirmed in larger studies.
publishDate 2021
dc.date.none.fl_str_mv 2021-11-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/170558
Brossas, Jean Yves; Ballering, Griselda Edith; Bisio, Margarita María Catalina; Guihenneuc, Jeremy; Gulin, Julián Ernesto Nicolás; et al.; Evaluation of the chagas western blot igg assay for the diagnosis of chagas disease; MDPI; Pathogens; 10; 11; 10-11-2021; 1-10
2076-0817
CONICET Digital
CONICET
url http://hdl.handle.net/11336/170558
identifier_str_mv Brossas, Jean Yves; Ballering, Griselda Edith; Bisio, Margarita María Catalina; Guihenneuc, Jeremy; Gulin, Julián Ernesto Nicolás; et al.; Evaluation of the chagas western blot igg assay for the diagnosis of chagas disease; MDPI; Pathogens; 10; 11; 10-11-2021; 1-10
2076-0817
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/pathogens10111455
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/22/5/2414
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.001348

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