The Trypanosoma cruzi Satellite DNA OligoC-TesT and Trypanosoma cruzi Kinetoplast DNA OligoC-TesT for Diagnosis of Chagas Disease: A Multi-cohort Comparative Evaluation Study

Autores
de Winne, Koen; Büscher, Philippe; Luquetti, Alejandro O.; Tavares, Suelene B. N.; Oliveira, Rodrigo A.; Solari, Aldo; Zulantay, Ines; Apt, Werner; Diosque, Patricio; Monje Rumi, Maria Mercedes; Gironés, Nuria; Fresno, Manuel; Lopez Velez, Rogelio; Perez Molina, José A.; Monge Maillo, Begoña; Garcia, Lineth; Deborggraeve, Stijn
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: The Trypanosoma cruzi satellite DNA (satDNA) OligoC-TesT is a standardised PCR format for diagnosis of Chagas disease. The sensitivity of the test is lower for discrete typing unit (DTU) TcI than for TcII-VI and the test has not been evaluated in chronic Chagas disease patients. Methodology/Principal Findings: We developed a new prototype of the OligoC-TesT based on kinetoplast DNA (kDNA) detection. We evaluated the satDNA and kDNA OligoC-TesTs in a multi-cohort study with 187 chronic Chagas patients and 88 healthy endemic controls recruited in Argentina, Chile and Spain and 26 diseased non-endemic controls from D.R. Congo and Sudan. All specimens were tested in duplicate. The overall specificity in the controls was 99.1% (95% CI 95.2%-99.8%) for the satDNA OligoC-TesT and 97.4% (95% CI 92.6%?99.1%) for the kDNA OligoC-TesT. The overall sensitivity in the patients was 67.9% (95% CI 60.9%-74.2%) for the satDNA OligoC-TesT and 79.1% (95% CI 72.8%-84.4%) for the kDNA OligoC-Test. Conclusions/Significance: Specificities of the two T. cruzi OligoC-TesT prototypes are high on non-endemic and endemic controls. Sensitivities are moderate but significantly (p= 0.0004) higher for the kDNA OligoC-TesT compared to the satDNA OligoC-TesT.
Fil: de Winne, Koen . Institute of Tropical Medicine; Bélgica
Fil: Büscher, Philippe . Institute of Tropical Medicine; Bélgica
Fil: Luquetti, Alejandro O. . Universidade Federal de Goias; Brasil
Fil: Tavares, Suelene B. N. . Universidade Federal de Goias; Brasil
Fil: Oliveira, Rodrigo A. . Universidade Federal de Goias; Brasil
Fil: Solari, Aldo . Universidad de Santiago de Chile; Chile
Fil: Zulantay, Ines . Universidad de Santiago de Chile; Chile
Fil: Apt, Werner . Universidad de Santiago de Chile; Chile
Fil: Diosque, Patricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta; Argentina
Fil: Monje Rumi, Maria Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta; Argentina
Fil: Gironés, Nuria . Consejo Superior de Investigaciones Cientificas; España
Fil: Fresno, Manuel . Consejo Superior de Investigaciones Cientificas; España
Fil: Lopez Velez, Rogelio. Ramon y Cajal Hospital, Madrid; España
Fil: Perez Molina, José A. . Ramon y Cajal Hospital, Madrid; España
Fil: Monge Maillo, Begoña . Ramon y Cajal Hospital, Madrid; España
Fil: Garcia, Lineth . Universidad Mayor de San Simon; Bolivia
Fil: Deborggraeve, Stijn . Institute of Tropical Medicine; Bélgica
Materia
TRYPANOSOMA CRUZI
DIAGNOSTIC
CHAGAS DISEASE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7092
Acceder
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network_name_str CONICET Digital (CONICET)
spelling The Trypanosoma cruzi Satellite DNA OligoC-TesT and Trypanosoma cruzi Kinetoplast DNA OligoC-TesT for Diagnosis of Chagas Disease: A Multi-cohort Comparative Evaluation Studyde Winne, Koen Büscher, Philippe Luquetti, Alejandro O. Tavares, Suelene B. N. Oliveira, Rodrigo A. Solari, Aldo Zulantay, Ines Apt, Werner Diosque, PatricioMonje Rumi, Maria MercedesGironés, Nuria Fresno, Manuel Lopez Velez, RogelioPerez Molina, José A. Monge Maillo, Begoña Garcia, Lineth Deborggraeve, Stijn TRYPANOSOMA CRUZIDIAGNOSTICCHAGAS DISEASEhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Background: The Trypanosoma cruzi satellite DNA (satDNA) OligoC-TesT is a standardised PCR format for diagnosis of Chagas disease. The sensitivity of the test is lower for discrete typing unit (DTU) TcI than for TcII-VI and the test has not been evaluated in chronic Chagas disease patients. Methodology/Principal Findings: We developed a new prototype of the OligoC-TesT based on kinetoplast DNA (kDNA) detection. We evaluated the satDNA and kDNA OligoC-TesTs in a multi-cohort study with 187 chronic Chagas patients and 88 healthy endemic controls recruited in Argentina, Chile and Spain and 26 diseased non-endemic controls from D.R. Congo and Sudan. All specimens were tested in duplicate. The overall specificity in the controls was 99.1% (95% CI 95.2%-99.8%) for the satDNA OligoC-TesT and 97.4% (95% CI 92.6%?99.1%) for the kDNA OligoC-TesT. The overall sensitivity in the patients was 67.9% (95% CI 60.9%-74.2%) for the satDNA OligoC-TesT and 79.1% (95% CI 72.8%-84.4%) for the kDNA OligoC-Test. Conclusions/Significance: Specificities of the two T. cruzi OligoC-TesT prototypes are high on non-endemic and endemic controls. Sensitivities are moderate but significantly (p= 0.0004) higher for the kDNA OligoC-TesT compared to the satDNA OligoC-TesT.Fil: de Winne, Koen . Institute of Tropical Medicine; BélgicaFil: Büscher, Philippe . Institute of Tropical Medicine; BélgicaFil: Luquetti, Alejandro O. . Universidade Federal de Goias; BrasilFil: Tavares, Suelene B. N. . Universidade Federal de Goias; BrasilFil: Oliveira, Rodrigo A. . Universidade Federal de Goias; BrasilFil: Solari, Aldo . Universidad de Santiago de Chile; ChileFil: Zulantay, Ines . Universidad de Santiago de Chile; ChileFil: Apt, Werner . Universidad de Santiago de Chile; ChileFil: Diosque, Patricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta; ArgentinaFil: Monje Rumi, Maria Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta; ArgentinaFil: Gironés, Nuria . Consejo Superior de Investigaciones Cientificas; EspañaFil: Fresno, Manuel . Consejo Superior de Investigaciones Cientificas; EspañaFil: Lopez Velez, Rogelio. Ramon y Cajal Hospital, Madrid; EspañaFil: Perez Molina, José A. . Ramon y Cajal Hospital, Madrid; EspañaFil: Monge Maillo, Begoña . Ramon y Cajal Hospital, Madrid; EspañaFil: Garcia, Lineth . Universidad Mayor de San Simon; BoliviaFil: Deborggraeve, Stijn . Institute of Tropical Medicine; BélgicaPublic Library Of Science2014-01-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7092de Winne, Koen ; Büscher, Philippe ; Luquetti, Alejandro O. ; Tavares, Suelene B. N. ; Oliveira, Rodrigo A. ; et al.; The Trypanosoma cruzi Satellite DNA OligoC-TesT and Trypanosoma cruzi Kinetoplast DNA OligoC-TesT for Diagnosis of Chagas Disease: A Multi-cohort Comparative Evaluation Study; Public Library Of Science; Neglected Tropical Diseases; 8; 1; 2-1-2014; 2633-26331935-2735enginfo:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/pmid/PMC3879245info:eu-repo/semantics/altIdentifier/doi/10.1371/ journal.pntd.0002633info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3879245/info:eu-repo/semantics/altIdentifier/url/http://www.plosntds.org/article/info%3Adoi%2F10.1371%2Fjournal.pntd.0002633info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:56:42Zoai:ri.conicet.gov.ar:11336/7092instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:56:42.637CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The Trypanosoma cruzi Satellite DNA OligoC-TesT and Trypanosoma cruzi Kinetoplast DNA OligoC-TesT for Diagnosis of Chagas Disease: A Multi-cohort Comparative Evaluation Study
title The Trypanosoma cruzi Satellite DNA OligoC-TesT and Trypanosoma cruzi Kinetoplast DNA OligoC-TesT for Diagnosis of Chagas Disease: A Multi-cohort Comparative Evaluation Study
spellingShingle The Trypanosoma cruzi Satellite DNA OligoC-TesT and Trypanosoma cruzi Kinetoplast DNA OligoC-TesT for Diagnosis of Chagas Disease: A Multi-cohort Comparative Evaluation Study
de Winne, Koen
TRYPANOSOMA CRUZI
DIAGNOSTIC
CHAGAS DISEASE
title_short The Trypanosoma cruzi Satellite DNA OligoC-TesT and Trypanosoma cruzi Kinetoplast DNA OligoC-TesT for Diagnosis of Chagas Disease: A Multi-cohort Comparative Evaluation Study
title_full The Trypanosoma cruzi Satellite DNA OligoC-TesT and Trypanosoma cruzi Kinetoplast DNA OligoC-TesT for Diagnosis of Chagas Disease: A Multi-cohort Comparative Evaluation Study
title_fullStr The Trypanosoma cruzi Satellite DNA OligoC-TesT and Trypanosoma cruzi Kinetoplast DNA OligoC-TesT for Diagnosis of Chagas Disease: A Multi-cohort Comparative Evaluation Study
title_full_unstemmed The Trypanosoma cruzi Satellite DNA OligoC-TesT and Trypanosoma cruzi Kinetoplast DNA OligoC-TesT for Diagnosis of Chagas Disease: A Multi-cohort Comparative Evaluation Study
title_sort The Trypanosoma cruzi Satellite DNA OligoC-TesT and Trypanosoma cruzi Kinetoplast DNA OligoC-TesT for Diagnosis of Chagas Disease: A Multi-cohort Comparative Evaluation Study
dc.creator.none.fl_str_mv de Winne, Koen
Büscher, Philippe
Luquetti, Alejandro O.
Tavares, Suelene B. N.
Oliveira, Rodrigo A.
Solari, Aldo
Zulantay, Ines
Apt, Werner
Diosque, Patricio
Monje Rumi, Maria Mercedes
Gironés, Nuria
Fresno, Manuel
Lopez Velez, Rogelio
Perez Molina, José A.
Monge Maillo, Begoña
Garcia, Lineth
Deborggraeve, Stijn
author de Winne, Koen
author_facet de Winne, Koen
Büscher, Philippe
Luquetti, Alejandro O.
Tavares, Suelene B. N.
Oliveira, Rodrigo A.
Solari, Aldo
Zulantay, Ines
Apt, Werner
Diosque, Patricio
Monje Rumi, Maria Mercedes
Gironés, Nuria
Fresno, Manuel
Lopez Velez, Rogelio
Perez Molina, José A.
Monge Maillo, Begoña
Garcia, Lineth
Deborggraeve, Stijn
author_role author
author2 Büscher, Philippe
Luquetti, Alejandro O.
Tavares, Suelene B. N.
Oliveira, Rodrigo A.
Solari, Aldo
Zulantay, Ines
Apt, Werner
Diosque, Patricio
Monje Rumi, Maria Mercedes
Gironés, Nuria
Fresno, Manuel
Lopez Velez, Rogelio
Perez Molina, José A.
Monge Maillo, Begoña
Garcia, Lineth
Deborggraeve, Stijn
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv TRYPANOSOMA CRUZI
DIAGNOSTIC
CHAGAS DISEASE
topic TRYPANOSOMA CRUZI
DIAGNOSTIC
CHAGAS DISEASE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background: The Trypanosoma cruzi satellite DNA (satDNA) OligoC-TesT is a standardised PCR format for diagnosis of Chagas disease. The sensitivity of the test is lower for discrete typing unit (DTU) TcI than for TcII-VI and the test has not been evaluated in chronic Chagas disease patients. Methodology/Principal Findings: We developed a new prototype of the OligoC-TesT based on kinetoplast DNA (kDNA) detection. We evaluated the satDNA and kDNA OligoC-TesTs in a multi-cohort study with 187 chronic Chagas patients and 88 healthy endemic controls recruited in Argentina, Chile and Spain and 26 diseased non-endemic controls from D.R. Congo and Sudan. All specimens were tested in duplicate. The overall specificity in the controls was 99.1% (95% CI 95.2%-99.8%) for the satDNA OligoC-TesT and 97.4% (95% CI 92.6%?99.1%) for the kDNA OligoC-TesT. The overall sensitivity in the patients was 67.9% (95% CI 60.9%-74.2%) for the satDNA OligoC-TesT and 79.1% (95% CI 72.8%-84.4%) for the kDNA OligoC-Test. Conclusions/Significance: Specificities of the two T. cruzi OligoC-TesT prototypes are high on non-endemic and endemic controls. Sensitivities are moderate but significantly (p= 0.0004) higher for the kDNA OligoC-TesT compared to the satDNA OligoC-TesT.
Fil: de Winne, Koen . Institute of Tropical Medicine; Bélgica
Fil: Büscher, Philippe . Institute of Tropical Medicine; Bélgica
Fil: Luquetti, Alejandro O. . Universidade Federal de Goias; Brasil
Fil: Tavares, Suelene B. N. . Universidade Federal de Goias; Brasil
Fil: Oliveira, Rodrigo A. . Universidade Federal de Goias; Brasil
Fil: Solari, Aldo . Universidad de Santiago de Chile; Chile
Fil: Zulantay, Ines . Universidad de Santiago de Chile; Chile
Fil: Apt, Werner . Universidad de Santiago de Chile; Chile
Fil: Diosque, Patricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta; Argentina
Fil: Monje Rumi, Maria Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta; Argentina
Fil: Gironés, Nuria . Consejo Superior de Investigaciones Cientificas; España
Fil: Fresno, Manuel . Consejo Superior de Investigaciones Cientificas; España
Fil: Lopez Velez, Rogelio. Ramon y Cajal Hospital, Madrid; España
Fil: Perez Molina, José A. . Ramon y Cajal Hospital, Madrid; España
Fil: Monge Maillo, Begoña . Ramon y Cajal Hospital, Madrid; España
Fil: Garcia, Lineth . Universidad Mayor de San Simon; Bolivia
Fil: Deborggraeve, Stijn . Institute of Tropical Medicine; Bélgica
description Background: The Trypanosoma cruzi satellite DNA (satDNA) OligoC-TesT is a standardised PCR format for diagnosis of Chagas disease. The sensitivity of the test is lower for discrete typing unit (DTU) TcI than for TcII-VI and the test has not been evaluated in chronic Chagas disease patients. Methodology/Principal Findings: We developed a new prototype of the OligoC-TesT based on kinetoplast DNA (kDNA) detection. We evaluated the satDNA and kDNA OligoC-TesTs in a multi-cohort study with 187 chronic Chagas patients and 88 healthy endemic controls recruited in Argentina, Chile and Spain and 26 diseased non-endemic controls from D.R. Congo and Sudan. All specimens were tested in duplicate. The overall specificity in the controls was 99.1% (95% CI 95.2%-99.8%) for the satDNA OligoC-TesT and 97.4% (95% CI 92.6%?99.1%) for the kDNA OligoC-TesT. The overall sensitivity in the patients was 67.9% (95% CI 60.9%-74.2%) for the satDNA OligoC-TesT and 79.1% (95% CI 72.8%-84.4%) for the kDNA OligoC-Test. Conclusions/Significance: Specificities of the two T. cruzi OligoC-TesT prototypes are high on non-endemic and endemic controls. Sensitivities are moderate but significantly (p= 0.0004) higher for the kDNA OligoC-TesT compared to the satDNA OligoC-TesT.
publishDate 2014
dc.date.none.fl_str_mv 2014-01-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7092
de Winne, Koen ; Büscher, Philippe ; Luquetti, Alejandro O. ; Tavares, Suelene B. N. ; Oliveira, Rodrigo A. ; et al.; The Trypanosoma cruzi Satellite DNA OligoC-TesT and Trypanosoma cruzi Kinetoplast DNA OligoC-TesT for Diagnosis of Chagas Disease: A Multi-cohort Comparative Evaluation Study; Public Library Of Science; Neglected Tropical Diseases; 8; 1; 2-1-2014; 2633-2633
1935-2735
url http://hdl.handle.net/11336/7092
identifier_str_mv de Winne, Koen ; Büscher, Philippe ; Luquetti, Alejandro O. ; Tavares, Suelene B. N. ; Oliveira, Rodrigo A. ; et al.; The Trypanosoma cruzi Satellite DNA OligoC-TesT and Trypanosoma cruzi Kinetoplast DNA OligoC-TesT for Diagnosis of Chagas Disease: A Multi-cohort Comparative Evaluation Study; Public Library Of Science; Neglected Tropical Diseases; 8; 1; 2-1-2014; 2633-2633
1935-2735
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/pmid/PMC3879245
info:eu-repo/semantics/altIdentifier/doi/10.1371/ journal.pntd.0002633
info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3879245/
info:eu-repo/semantics/altIdentifier/url/http://www.plosntds.org/article/info%3Adoi%2F10.1371%2Fjournal.pntd.0002633
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library Of Science
publisher.none.fl_str_mv Public Library Of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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