Antigenicity and diagnostic potential of vaccine candidates in human Chagas disease

Autores
Gupta, Shivali; Wan, Xianxu; Zago, María Paola; Martinez Sellers, Valena C.; Silva, Trevor S.; Assiah, Dadjah; Dhiman, Monisha; Nuñez, Sonia; Petersen, John R.; Vazquez Chagoyán, Juan C.; Estrada Franco, Jose G.; Garg, Nisha Jain
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Chagas disease is the most common cause of congestive heart failure related deaths among young adults in the endemic areas of South and Central America and Mexico. Diagnosis and treatment of T. cruzi infection has remained difficult and challenging after 100 years of its identification. In >95% of human cases, T. cruzi infection remains undiagnosed until several years later when chronic evolution of progressive disease results in clinical symptoms associated with cardiac damage. Diagnosis generally depends on the measurement of T. cruzi'specific antibodies that can result in false positives. A conclusive diagnosis of T. cruzi infection thus often requires multiple serological tests, in combination with epidemiological data and clinical symptoms. In this study, we investigated the antibody response to TcG1, TcG2, and TcG4 in clinically characterized chagasic patients. These antigens were identified as vaccine candidates and shown to elicit protective immunity to T. cruzi and Chagas disease in experimental animals. Our data show the serology test developed using the TcGmix (multiplex ELISA) is a significantly better alternative to epimastigote extracts currently used in T. cruzi serodiagnosis or the trypomastigote lysate used in this study for comparison purposes.
Fil: Gupta, Shivali. University Of Texas Medical Branch. Department Of Pathology; Estados Unidos
Fil: Wan, Xianxu. University Of Texas Medical Branch. Department Of Pathology; Estados Unidos
Fil: Zago, María Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina
Fil: Martinez Sellers, Valena C.. University Of Texas Medical Branch. Department of Pathology; Estados Unidos
Fil: Silva, Trevor S.. University Of Texas Medical Branch. Department of Pathology; Estados Unidos
Fil: Assiah, Dadjah. University Of Texas Medical Branch. Department of Pathology; Estados Unidos
Fil: Dhiman, Monisha. University Of Texas Medical Branch. Department of Pathology; Estados Unidos
Fil: Nuñez, Sonia. Provincia de Salta. Hospital Público de Gestion Descentralizada San Bernardo; Argentina
Fil: Petersen, John R.. University Of Texas Medical Branch. Department of Pathology; Estados Unidos
Fil: Vazquez Chagoyán, Juan C.. Universidad Autónoma de Estado de México ; México
Fil: Estrada Franco, Jose G.. Universidad Autónoma de Estado de México; México
Fil: Garg, Nisha Jain. University Of Texas Medical Branch. Department of Pathology; Estados Unidos
Materia
TRYPANOSOMA CRUZI
DIAGNOSTIC
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7617
Acceder
id CONICETDig_e7c07cfa74ced2e683de87cb87ca134d
oai_identifier_str oai:ri.conicet.gov.ar:11336/7617
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antigenicity and diagnostic potential of vaccine candidates in human Chagas diseaseGupta, ShivaliWan, XianxuZago, María PaolaMartinez Sellers, Valena C.Silva, Trevor S.Assiah, DadjahDhiman, MonishaNuñez, SoniaPetersen, John R.Vazquez Chagoyán, Juan C.Estrada Franco, Jose G.Garg, Nisha JainTRYPANOSOMA CRUZIDIAGNOSTIChttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Chagas disease is the most common cause of congestive heart failure related deaths among young adults in the endemic areas of South and Central America and Mexico. Diagnosis and treatment of T. cruzi infection has remained difficult and challenging after 100 years of its identification. In >95% of human cases, T. cruzi infection remains undiagnosed until several years later when chronic evolution of progressive disease results in clinical symptoms associated with cardiac damage. Diagnosis generally depends on the measurement of T. cruzi'specific antibodies that can result in false positives. A conclusive diagnosis of T. cruzi infection thus often requires multiple serological tests, in combination with epidemiological data and clinical symptoms. In this study, we investigated the antibody response to TcG1, TcG2, and TcG4 in clinically characterized chagasic patients. These antigens were identified as vaccine candidates and shown to elicit protective immunity to T. cruzi and Chagas disease in experimental animals. Our data show the serology test developed using the TcGmix (multiplex ELISA) is a significantly better alternative to epimastigote extracts currently used in T. cruzi serodiagnosis or the trypomastigote lysate used in this study for comparison purposes.Fil: Gupta, Shivali. University Of Texas Medical Branch. Department Of Pathology; Estados UnidosFil: Wan, Xianxu. University Of Texas Medical Branch. Department Of Pathology; Estados UnidosFil: Zago, María Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; ArgentinaFil: Martinez Sellers, Valena C.. University Of Texas Medical Branch. Department of Pathology; Estados UnidosFil: Silva, Trevor S.. University Of Texas Medical Branch. Department of Pathology; Estados UnidosFil: Assiah, Dadjah. University Of Texas Medical Branch. Department of Pathology; Estados UnidosFil: Dhiman, Monisha. University Of Texas Medical Branch. Department of Pathology; Estados UnidosFil: Nuñez, Sonia. Provincia de Salta. Hospital Público de Gestion Descentralizada San Bernardo; ArgentinaFil: Petersen, John R.. University Of Texas Medical Branch. Department of Pathology; Estados UnidosFil: Vazquez Chagoyán, Juan C.. Universidad Autónoma de Estado de México ; MéxicoFil: Estrada Franco, Jose G.. Universidad Autónoma de Estado de México; MéxicoFil: Garg, Nisha Jain. University Of Texas Medical Branch. Department of Pathology; Estados UnidosPublic Library Of Science2013-01-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7617Gupta, Shivali; Wan, Xianxu; Zago, María Paola; Martinez Sellers, Valena C.; Silva, Trevor S.; et al.; Antigenicity and diagnostic potential of vaccine candidates in human Chagas disease; Public Library Of Science; Neglected Tropical Diseases; 7; 1; 17-1-2013; 1-101935-2735enginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pntd.0002018info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3547861/info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0002018info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:44:39Zoai:ri.conicet.gov.ar:11336/7617instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:44:40.077CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antigenicity and diagnostic potential of vaccine candidates in human Chagas disease
title Antigenicity and diagnostic potential of vaccine candidates in human Chagas disease
spellingShingle Antigenicity and diagnostic potential of vaccine candidates in human Chagas disease
Gupta, Shivali
TRYPANOSOMA CRUZI
DIAGNOSTIC
title_short Antigenicity and diagnostic potential of vaccine candidates in human Chagas disease
title_full Antigenicity and diagnostic potential of vaccine candidates in human Chagas disease
title_fullStr Antigenicity and diagnostic potential of vaccine candidates in human Chagas disease
title_full_unstemmed Antigenicity and diagnostic potential of vaccine candidates in human Chagas disease
title_sort Antigenicity and diagnostic potential of vaccine candidates in human Chagas disease
dc.creator.none.fl_str_mv Gupta, Shivali
Wan, Xianxu
Zago, María Paola
Martinez Sellers, Valena C.
Silva, Trevor S.
Assiah, Dadjah
Dhiman, Monisha
Nuñez, Sonia
Petersen, John R.
Vazquez Chagoyán, Juan C.
Estrada Franco, Jose G.
Garg, Nisha Jain
author Gupta, Shivali
author_facet Gupta, Shivali
Wan, Xianxu
Zago, María Paola
Martinez Sellers, Valena C.
Silva, Trevor S.
Assiah, Dadjah
Dhiman, Monisha
Nuñez, Sonia
Petersen, John R.
Vazquez Chagoyán, Juan C.
Estrada Franco, Jose G.
Garg, Nisha Jain
author_role author
author2 Wan, Xianxu
Zago, María Paola
Martinez Sellers, Valena C.
Silva, Trevor S.
Assiah, Dadjah
Dhiman, Monisha
Nuñez, Sonia
Petersen, John R.
Vazquez Chagoyán, Juan C.
Estrada Franco, Jose G.
Garg, Nisha Jain
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv TRYPANOSOMA CRUZI
DIAGNOSTIC
topic TRYPANOSOMA CRUZI
DIAGNOSTIC
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Chagas disease is the most common cause of congestive heart failure related deaths among young adults in the endemic areas of South and Central America and Mexico. Diagnosis and treatment of T. cruzi infection has remained difficult and challenging after 100 years of its identification. In >95% of human cases, T. cruzi infection remains undiagnosed until several years later when chronic evolution of progressive disease results in clinical symptoms associated with cardiac damage. Diagnosis generally depends on the measurement of T. cruzi'specific antibodies that can result in false positives. A conclusive diagnosis of T. cruzi infection thus often requires multiple serological tests, in combination with epidemiological data and clinical symptoms. In this study, we investigated the antibody response to TcG1, TcG2, and TcG4 in clinically characterized chagasic patients. These antigens were identified as vaccine candidates and shown to elicit protective immunity to T. cruzi and Chagas disease in experimental animals. Our data show the serology test developed using the TcGmix (multiplex ELISA) is a significantly better alternative to epimastigote extracts currently used in T. cruzi serodiagnosis or the trypomastigote lysate used in this study for comparison purposes.
Fil: Gupta, Shivali. University Of Texas Medical Branch. Department Of Pathology; Estados Unidos
Fil: Wan, Xianxu. University Of Texas Medical Branch. Department Of Pathology; Estados Unidos
Fil: Zago, María Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina
Fil: Martinez Sellers, Valena C.. University Of Texas Medical Branch. Department of Pathology; Estados Unidos
Fil: Silva, Trevor S.. University Of Texas Medical Branch. Department of Pathology; Estados Unidos
Fil: Assiah, Dadjah. University Of Texas Medical Branch. Department of Pathology; Estados Unidos
Fil: Dhiman, Monisha. University Of Texas Medical Branch. Department of Pathology; Estados Unidos
Fil: Nuñez, Sonia. Provincia de Salta. Hospital Público de Gestion Descentralizada San Bernardo; Argentina
Fil: Petersen, John R.. University Of Texas Medical Branch. Department of Pathology; Estados Unidos
Fil: Vazquez Chagoyán, Juan C.. Universidad Autónoma de Estado de México ; México
Fil: Estrada Franco, Jose G.. Universidad Autónoma de Estado de México; México
Fil: Garg, Nisha Jain. University Of Texas Medical Branch. Department of Pathology; Estados Unidos
description Chagas disease is the most common cause of congestive heart failure related deaths among young adults in the endemic areas of South and Central America and Mexico. Diagnosis and treatment of T. cruzi infection has remained difficult and challenging after 100 years of its identification. In >95% of human cases, T. cruzi infection remains undiagnosed until several years later when chronic evolution of progressive disease results in clinical symptoms associated with cardiac damage. Diagnosis generally depends on the measurement of T. cruzi'specific antibodies that can result in false positives. A conclusive diagnosis of T. cruzi infection thus often requires multiple serological tests, in combination with epidemiological data and clinical symptoms. In this study, we investigated the antibody response to TcG1, TcG2, and TcG4 in clinically characterized chagasic patients. These antigens were identified as vaccine candidates and shown to elicit protective immunity to T. cruzi and Chagas disease in experimental animals. Our data show the serology test developed using the TcGmix (multiplex ELISA) is a significantly better alternative to epimastigote extracts currently used in T. cruzi serodiagnosis or the trypomastigote lysate used in this study for comparison purposes.
publishDate 2013
dc.date.none.fl_str_mv 2013-01-17
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7617
Gupta, Shivali; Wan, Xianxu; Zago, María Paola; Martinez Sellers, Valena C.; Silva, Trevor S.; et al.; Antigenicity and diagnostic potential of vaccine candidates in human Chagas disease; Public Library Of Science; Neglected Tropical Diseases; 7; 1; 17-1-2013; 1-10
1935-2735
url http://hdl.handle.net/11336/7617
identifier_str_mv Gupta, Shivali; Wan, Xianxu; Zago, María Paola; Martinez Sellers, Valena C.; Silva, Trevor S.; et al.; Antigenicity and diagnostic potential of vaccine candidates in human Chagas disease; Public Library Of Science; Neglected Tropical Diseases; 7; 1; 17-1-2013; 1-10
1935-2735
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pntd.0002018
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3547861/
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0002018
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library Of Science
publisher.none.fl_str_mv Public Library Of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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