Genetic variation in the E6 and E7 genes of human papillomavirus type 16 in northeastern Argentina
- Autores
- Totaro, María Elina; Gili, Juan Antonio; Liotta, Domingo Javier; Schurr, Theodore; Picconi, María Alejandra; Badano, Ines
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The province of Misiones is considered a region with a high mortality rate due to cervical cancer (CC). To gain insight into this problem, we explored the association between genetic variation in the E6 and E7 oncogenes of HPV16 and the risk of CC. We studied 160 women with cytological diagnoses of negative for intraepithelial lesion or malignity, low-grade squamous intraepithelial lesion, and high-grade squamous intraepithelial lesion/CC and a positive test for HPV16 infection. The genetic characterization of E6 and E7 genes was undertaken through PCR amplification and direct Sanger sequencing. Phylogenetic classification was conducted using Bayesian methods. To estimate the odds ratio (OR) for an association between genetic variants in the E6 and E7 genes and the risk of CC, we used ordinal logistic regression adjusted by age. The final data set comprised 112 samples. Diagnostic single-nucleotide polymorphisms (SNPs) and phylogenetic trees confirmed the presence of Lineage A (95.5%) and D (4.5%) in the samples. For the E6 gene, we identified eleven different sequences, with the most common ones being Lineage A E6 350G (58.9%) and E6 350T (37.5%). The E6 350G was associated with progression to HSIL/CC, with an OR of 19.41 (4.95–76.10). The E7 gene was more conserved than E6, probably due to the functional constraints of this small protein. Our results confirmed the association of the E6 350G SNP with a higher risk of developing CC. These data will contribute to understanding the biological bases of CC incidence in this region.
Fil: Totaro, María Elina. Universidad Nacional de Misiones; Argentina
Fil: Gili, Juan Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Educación Médica e Investigaciones Clínicas "Norberto Quirno"; Argentina
Fil: Liotta, Domingo Javier. Universidad Nacional de Misiones; Argentina. Dirección Nacional de Instituto de Investigación.Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Schurr, Theodore. University of Pennsylvania; Estados Unidos
Fil: Picconi, María Alejandra. Dirección Nacional de Instituto de Investigación.Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"; Argentina
Fil: Badano, Ines. Universidad Nacional de Misiones; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina - Materia
-
CARCINOGENESIS
CERVICAL CANCER
HPV
RISK FACTOR
SNP - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/167479
Ver los metadatos del registro completo
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Genetic variation in the E6 and E7 genes of human papillomavirus type 16 in northeastern ArgentinaTotaro, María ElinaGili, Juan AntonioLiotta, Domingo JavierSchurr, TheodorePicconi, María AlejandraBadano, InesCARCINOGENESISCERVICAL CANCERHPVRISK FACTORSNPhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The province of Misiones is considered a region with a high mortality rate due to cervical cancer (CC). To gain insight into this problem, we explored the association between genetic variation in the E6 and E7 oncogenes of HPV16 and the risk of CC. We studied 160 women with cytological diagnoses of negative for intraepithelial lesion or malignity, low-grade squamous intraepithelial lesion, and high-grade squamous intraepithelial lesion/CC and a positive test for HPV16 infection. The genetic characterization of E6 and E7 genes was undertaken through PCR amplification and direct Sanger sequencing. Phylogenetic classification was conducted using Bayesian methods. To estimate the odds ratio (OR) for an association between genetic variants in the E6 and E7 genes and the risk of CC, we used ordinal logistic regression adjusted by age. The final data set comprised 112 samples. Diagnostic single-nucleotide polymorphisms (SNPs) and phylogenetic trees confirmed the presence of Lineage A (95.5%) and D (4.5%) in the samples. For the E6 gene, we identified eleven different sequences, with the most common ones being Lineage A E6 350G (58.9%) and E6 350T (37.5%). The E6 350G was associated with progression to HSIL/CC, with an OR of 19.41 (4.95–76.10). The E7 gene was more conserved than E6, probably due to the functional constraints of this small protein. Our results confirmed the association of the E6 350G SNP with a higher risk of developing CC. These data will contribute to understanding the biological bases of CC incidence in this region.Fil: Totaro, María Elina. Universidad Nacional de Misiones; ArgentinaFil: Gili, Juan Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Educación Médica e Investigaciones Clínicas "Norberto Quirno"; ArgentinaFil: Liotta, Domingo Javier. Universidad Nacional de Misiones; Argentina. Dirección Nacional de Instituto de Investigación.Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Schurr, Theodore. University of Pennsylvania; Estados UnidosFil: Picconi, María Alejandra. Dirección Nacional de Instituto de Investigación.Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"; ArgentinaFil: Badano, Ines. Universidad Nacional de Misiones; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaWiley-liss, div John Wiley & Sons Inc.2021-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/167479Totaro, María Elina; Gili, Juan Antonio; Liotta, Domingo Javier; Schurr, Theodore; Picconi, María Alejandra; et al.; Genetic variation in the E6 and E7 genes of human papillomavirus type 16 in northeastern Argentina; Wiley-liss, div John Wiley & Sons Inc.; Journal of Medical Virology; 94; 2; 9-2021; 745-7510146-6615CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/jmv.27359info:eu-repo/semantics/altIdentifier/doi/10.1002/jmv.27359info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:10:23Zoai:ri.conicet.gov.ar:11336/167479instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:10:23.736CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Genetic variation in the E6 and E7 genes of human papillomavirus type 16 in northeastern Argentina |
title |
Genetic variation in the E6 and E7 genes of human papillomavirus type 16 in northeastern Argentina |
spellingShingle |
Genetic variation in the E6 and E7 genes of human papillomavirus type 16 in northeastern Argentina Totaro, María Elina CARCINOGENESIS CERVICAL CANCER HPV RISK FACTOR SNP |
title_short |
Genetic variation in the E6 and E7 genes of human papillomavirus type 16 in northeastern Argentina |
title_full |
Genetic variation in the E6 and E7 genes of human papillomavirus type 16 in northeastern Argentina |
title_fullStr |
Genetic variation in the E6 and E7 genes of human papillomavirus type 16 in northeastern Argentina |
title_full_unstemmed |
Genetic variation in the E6 and E7 genes of human papillomavirus type 16 in northeastern Argentina |
title_sort |
Genetic variation in the E6 and E7 genes of human papillomavirus type 16 in northeastern Argentina |
dc.creator.none.fl_str_mv |
Totaro, María Elina Gili, Juan Antonio Liotta, Domingo Javier Schurr, Theodore Picconi, María Alejandra Badano, Ines |
author |
Totaro, María Elina |
author_facet |
Totaro, María Elina Gili, Juan Antonio Liotta, Domingo Javier Schurr, Theodore Picconi, María Alejandra Badano, Ines |
author_role |
author |
author2 |
Gili, Juan Antonio Liotta, Domingo Javier Schurr, Theodore Picconi, María Alejandra Badano, Ines |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
CARCINOGENESIS CERVICAL CANCER HPV RISK FACTOR SNP |
topic |
CARCINOGENESIS CERVICAL CANCER HPV RISK FACTOR SNP |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
The province of Misiones is considered a region with a high mortality rate due to cervical cancer (CC). To gain insight into this problem, we explored the association between genetic variation in the E6 and E7 oncogenes of HPV16 and the risk of CC. We studied 160 women with cytological diagnoses of negative for intraepithelial lesion or malignity, low-grade squamous intraepithelial lesion, and high-grade squamous intraepithelial lesion/CC and a positive test for HPV16 infection. The genetic characterization of E6 and E7 genes was undertaken through PCR amplification and direct Sanger sequencing. Phylogenetic classification was conducted using Bayesian methods. To estimate the odds ratio (OR) for an association between genetic variants in the E6 and E7 genes and the risk of CC, we used ordinal logistic regression adjusted by age. The final data set comprised 112 samples. Diagnostic single-nucleotide polymorphisms (SNPs) and phylogenetic trees confirmed the presence of Lineage A (95.5%) and D (4.5%) in the samples. For the E6 gene, we identified eleven different sequences, with the most common ones being Lineage A E6 350G (58.9%) and E6 350T (37.5%). The E6 350G was associated with progression to HSIL/CC, with an OR of 19.41 (4.95–76.10). The E7 gene was more conserved than E6, probably due to the functional constraints of this small protein. Our results confirmed the association of the E6 350G SNP with a higher risk of developing CC. These data will contribute to understanding the biological bases of CC incidence in this region. Fil: Totaro, María Elina. Universidad Nacional de Misiones; Argentina Fil: Gili, Juan Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Educación Médica e Investigaciones Clínicas "Norberto Quirno"; Argentina Fil: Liotta, Domingo Javier. Universidad Nacional de Misiones; Argentina. Dirección Nacional de Instituto de Investigación.Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina Fil: Schurr, Theodore. University of Pennsylvania; Estados Unidos Fil: Picconi, María Alejandra. Dirección Nacional de Instituto de Investigación.Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"; Argentina Fil: Badano, Ines. Universidad Nacional de Misiones; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina |
description |
The province of Misiones is considered a region with a high mortality rate due to cervical cancer (CC). To gain insight into this problem, we explored the association between genetic variation in the E6 and E7 oncogenes of HPV16 and the risk of CC. We studied 160 women with cytological diagnoses of negative for intraepithelial lesion or malignity, low-grade squamous intraepithelial lesion, and high-grade squamous intraepithelial lesion/CC and a positive test for HPV16 infection. The genetic characterization of E6 and E7 genes was undertaken through PCR amplification and direct Sanger sequencing. Phylogenetic classification was conducted using Bayesian methods. To estimate the odds ratio (OR) for an association between genetic variants in the E6 and E7 genes and the risk of CC, we used ordinal logistic regression adjusted by age. The final data set comprised 112 samples. Diagnostic single-nucleotide polymorphisms (SNPs) and phylogenetic trees confirmed the presence of Lineage A (95.5%) and D (4.5%) in the samples. For the E6 gene, we identified eleven different sequences, with the most common ones being Lineage A E6 350G (58.9%) and E6 350T (37.5%). The E6 350G was associated with progression to HSIL/CC, with an OR of 19.41 (4.95–76.10). The E7 gene was more conserved than E6, probably due to the functional constraints of this small protein. Our results confirmed the association of the E6 350G SNP with a higher risk of developing CC. These data will contribute to understanding the biological bases of CC incidence in this region. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-09 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/167479 Totaro, María Elina; Gili, Juan Antonio; Liotta, Domingo Javier; Schurr, Theodore; Picconi, María Alejandra; et al.; Genetic variation in the E6 and E7 genes of human papillomavirus type 16 in northeastern Argentina; Wiley-liss, div John Wiley & Sons Inc.; Journal of Medical Virology; 94; 2; 9-2021; 745-751 0146-6615 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/167479 |
identifier_str_mv |
Totaro, María Elina; Gili, Juan Antonio; Liotta, Domingo Javier; Schurr, Theodore; Picconi, María Alejandra; et al.; Genetic variation in the E6 and E7 genes of human papillomavirus type 16 in northeastern Argentina; Wiley-liss, div John Wiley & Sons Inc.; Journal of Medical Virology; 94; 2; 9-2021; 745-751 0146-6615 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/jmv.27359 info:eu-repo/semantics/altIdentifier/doi/10.1002/jmv.27359 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley-liss, div John Wiley & Sons Inc. |
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Wiley-liss, div John Wiley & Sons Inc. |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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