Genetic characterization and clinical implications of human papillomavirus type 16 (HPV16) variants from northeastern Argentina.

Autores
Badano, Ines; Totaro, María Elina; Culasso, Andrés Carlos Alberto; Sanabria, Daiana Jimena; Schurr, Theodore; Balette, Cristina Ileana; Roisman, Alejandro; Basiletti, Jorge Alejandro; Picconi, María Alejandra; Campos, Rodolfo Hector; Liotta, Domingo Javier
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
BACKGROUND: Human papillomavirus type 16 (HPV16) plays a central role in the development of cervical cancer. Worldwide studies indicate the existence of HPV16 variants that show different geographic distributions and oncogenic potential. OBJECTIVE: Our goal was to describe the genetic variation of HPV16 isolates identified in urban women with different grades of cervical lesions living in northeastern Argentina. STUDY DESIGN: We analyzed 116 HPV16-positive cervical samples (16 NLIM, 62 L-SIL, 16 H-SIL and 22 cervical cancer) from patients attending health centers in Misiones (Argentina) during 2006-13. HPV16 isolates were genetically characterized through PCR amplification and direct sequencing of 364 bp within the long control region, and the resulting sequences classified into variants based on phylogenetic analysis (lineages A, B, C and D). A potential association between HPV16 variants and lesion grade was evaluated through an odds ratio (OR) test. A temporal framework for the origin of HPV16 variants was assessed through coalescence analysis (BEAST v 1.7.5). RESULTS: Phylogenetic analysis of HPV16 sequences showed that 92.1% of the samples clustered with lineage A, and 6.9% to lineage D. HPV16 variants from lineage D were more frequently associated with high-grade lesions and cancer (HSIL+) than lineage A variants at an OR of 13.8 (1.6-117.0). The time to most common recent ancestor (tMCRA) of all variants was 119,103 years before present (HPD 95%=48,486-197,239), a date consistent with the time frame for modern human evolution. CONCLUSION: Our results suggest that HPV16 variants from lineage D may represent an additional risk factor for the development of cervical cancer in women living in northeastern Argentina. This study provides new information about viral isolates present in Argentina that will contribute to the monitoring of HPV16 infection in the vaccine era.
Fil: Badano, Ines. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina
Fil: Totaro, María Elina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina
Fil: Culasso, Andrés Carlos Alberto. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Virología; Argentina
Fil: Sanabria, Daiana Jimena. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina
Fil: Schurr, Theodore. University of Pennsylvania; Estados Unidos
Fil: Balette, Cristina Ileana. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina
Fil: Roisman, Alejandro. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina
Fil: Basiletti, Jorge Alejandro. Dirección Nacional de Instituto de Investigación. Adm.nacional de Laboratorio E Instituto de Salud "dr.c.g.malbran". Departamento Virus; Argentina
Fil: Picconi, María Alejandra. Dirección Nacional de Instituto de Investigación. Adm.nacional de Laboratorio E Instituto de Salud "dr.c.g.malbran". Departamento Virus; Argentina
Fil: Campos, Rodolfo Hector. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Virología; Argentina
Fil: Liotta, Domingo Javier. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina
Materia
Cervical Cáncer
Human Papillomavirus Type 16
Genetic Risk Factors
Phylogeny
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/29964
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/29964
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genetic characterization and clinical implications of human papillomavirus type 16 (HPV16) variants from northeastern Argentina.Badano, InesTotaro, María ElinaCulasso, Andrés Carlos AlbertoSanabria, Daiana JimenaSchurr, TheodoreBalette, Cristina IleanaRoisman, AlejandroBasiletti, Jorge AlejandroPicconi, María AlejandraCampos, Rodolfo HectorLiotta, Domingo JavierCervical CáncerHuman Papillomavirus Type 16Genetic Risk FactorsPhylogenyhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3BACKGROUND: Human papillomavirus type 16 (HPV16) plays a central role in the development of cervical cancer. Worldwide studies indicate the existence of HPV16 variants that show different geographic distributions and oncogenic potential. OBJECTIVE: Our goal was to describe the genetic variation of HPV16 isolates identified in urban women with different grades of cervical lesions living in northeastern Argentina. STUDY DESIGN: We analyzed 116 HPV16-positive cervical samples (16 NLIM, 62 L-SIL, 16 H-SIL and 22 cervical cancer) from patients attending health centers in Misiones (Argentina) during 2006-13. HPV16 isolates were genetically characterized through PCR amplification and direct sequencing of 364 bp within the long control region, and the resulting sequences classified into variants based on phylogenetic analysis (lineages A, B, C and D). A potential association between HPV16 variants and lesion grade was evaluated through an odds ratio (OR) test. A temporal framework for the origin of HPV16 variants was assessed through coalescence analysis (BEAST v 1.7.5). RESULTS: Phylogenetic analysis of HPV16 sequences showed that 92.1% of the samples clustered with lineage A, and 6.9% to lineage D. HPV16 variants from lineage D were more frequently associated with high-grade lesions and cancer (HSIL+) than lineage A variants at an OR of 13.8 (1.6-117.0). The time to most common recent ancestor (tMCRA) of all variants was 119,103 years before present (HPD 95%=48,486-197,239), a date consistent with the time frame for modern human evolution. CONCLUSION: Our results suggest that HPV16 variants from lineage D may represent an additional risk factor for the development of cervical cancer in women living in northeastern Argentina. This study provides new information about viral isolates present in Argentina that will contribute to the monitoring of HPV16 infection in the vaccine era.Fil: Badano, Ines. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; ArgentinaFil: Totaro, María Elina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; ArgentinaFil: Culasso, Andrés Carlos Alberto. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Virología; ArgentinaFil: Sanabria, Daiana Jimena. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; ArgentinaFil: Schurr, Theodore. University of Pennsylvania; Estados UnidosFil: Balette, Cristina Ileana. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; ArgentinaFil: Roisman, Alejandro. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; ArgentinaFil: Basiletti, Jorge Alejandro. Dirección Nacional de Instituto de Investigación. Adm.nacional de Laboratorio E Instituto de Salud "dr.c.g.malbran". Departamento Virus; ArgentinaFil: Picconi, María Alejandra. Dirección Nacional de Instituto de Investigación. Adm.nacional de Laboratorio E Instituto de Salud "dr.c.g.malbran". Departamento Virus; ArgentinaFil: Campos, Rodolfo Hector. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Virología; ArgentinaFil: Liotta, Domingo Javier. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; ArgentinaElsevier Science2015-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/29964Badano, Ines; Totaro, María Elina; Culasso, Andrés Carlos Alberto; Sanabria, Daiana Jimena; Schurr, Theodore; et al.; Genetic characterization and clinical implications of human papillomavirus type 16 (HPV16) variants from northeastern Argentina.; Elsevier Science; Infection, Genetics and Evolution; 29; 1-2015; 103-1091567-13481567-7257CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S156713481400416X?via%3Dihubinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.meegid.2014.11.013info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:07:13Zoai:ri.conicet.gov.ar:11336/29964instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:07:13.698CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genetic characterization and clinical implications of human papillomavirus type 16 (HPV16) variants from northeastern Argentina.
title Genetic characterization and clinical implications of human papillomavirus type 16 (HPV16) variants from northeastern Argentina.
spellingShingle Genetic characterization and clinical implications of human papillomavirus type 16 (HPV16) variants from northeastern Argentina.
Badano, Ines
Cervical Cáncer
Human Papillomavirus Type 16
Genetic Risk Factors
Phylogeny
title_short Genetic characterization and clinical implications of human papillomavirus type 16 (HPV16) variants from northeastern Argentina.
title_full Genetic characterization and clinical implications of human papillomavirus type 16 (HPV16) variants from northeastern Argentina.
title_fullStr Genetic characterization and clinical implications of human papillomavirus type 16 (HPV16) variants from northeastern Argentina.
title_full_unstemmed Genetic characterization and clinical implications of human papillomavirus type 16 (HPV16) variants from northeastern Argentina.
title_sort Genetic characterization and clinical implications of human papillomavirus type 16 (HPV16) variants from northeastern Argentina.
dc.creator.none.fl_str_mv Badano, Ines
Totaro, María Elina
Culasso, Andrés Carlos Alberto
Sanabria, Daiana Jimena
Schurr, Theodore
Balette, Cristina Ileana
Roisman, Alejandro
Basiletti, Jorge Alejandro
Picconi, María Alejandra
Campos, Rodolfo Hector
Liotta, Domingo Javier
author Badano, Ines
author_facet Badano, Ines
Totaro, María Elina
Culasso, Andrés Carlos Alberto
Sanabria, Daiana Jimena
Schurr, Theodore
Balette, Cristina Ileana
Roisman, Alejandro
Basiletti, Jorge Alejandro
Picconi, María Alejandra
Campos, Rodolfo Hector
Liotta, Domingo Javier
author_role author
author2 Totaro, María Elina
Culasso, Andrés Carlos Alberto
Sanabria, Daiana Jimena
Schurr, Theodore
Balette, Cristina Ileana
Roisman, Alejandro
Basiletti, Jorge Alejandro
Picconi, María Alejandra
Campos, Rodolfo Hector
Liotta, Domingo Javier
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cervical Cáncer
Human Papillomavirus Type 16
Genetic Risk Factors
Phylogeny
topic Cervical Cáncer
Human Papillomavirus Type 16
Genetic Risk Factors
Phylogeny
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv BACKGROUND: Human papillomavirus type 16 (HPV16) plays a central role in the development of cervical cancer. Worldwide studies indicate the existence of HPV16 variants that show different geographic distributions and oncogenic potential. OBJECTIVE: Our goal was to describe the genetic variation of HPV16 isolates identified in urban women with different grades of cervical lesions living in northeastern Argentina. STUDY DESIGN: We analyzed 116 HPV16-positive cervical samples (16 NLIM, 62 L-SIL, 16 H-SIL and 22 cervical cancer) from patients attending health centers in Misiones (Argentina) during 2006-13. HPV16 isolates were genetically characterized through PCR amplification and direct sequencing of 364 bp within the long control region, and the resulting sequences classified into variants based on phylogenetic analysis (lineages A, B, C and D). A potential association between HPV16 variants and lesion grade was evaluated through an odds ratio (OR) test. A temporal framework for the origin of HPV16 variants was assessed through coalescence analysis (BEAST v 1.7.5). RESULTS: Phylogenetic analysis of HPV16 sequences showed that 92.1% of the samples clustered with lineage A, and 6.9% to lineage D. HPV16 variants from lineage D were more frequently associated with high-grade lesions and cancer (HSIL+) than lineage A variants at an OR of 13.8 (1.6-117.0). The time to most common recent ancestor (tMCRA) of all variants was 119,103 years before present (HPD 95%=48,486-197,239), a date consistent with the time frame for modern human evolution. CONCLUSION: Our results suggest that HPV16 variants from lineage D may represent an additional risk factor for the development of cervical cancer in women living in northeastern Argentina. This study provides new information about viral isolates present in Argentina that will contribute to the monitoring of HPV16 infection in the vaccine era.
Fil: Badano, Ines. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina
Fil: Totaro, María Elina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina
Fil: Culasso, Andrés Carlos Alberto. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Virología; Argentina
Fil: Sanabria, Daiana Jimena. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina
Fil: Schurr, Theodore. University of Pennsylvania; Estados Unidos
Fil: Balette, Cristina Ileana. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina
Fil: Roisman, Alejandro. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina
Fil: Basiletti, Jorge Alejandro. Dirección Nacional de Instituto de Investigación. Adm.nacional de Laboratorio E Instituto de Salud "dr.c.g.malbran". Departamento Virus; Argentina
Fil: Picconi, María Alejandra. Dirección Nacional de Instituto de Investigación. Adm.nacional de Laboratorio E Instituto de Salud "dr.c.g.malbran". Departamento Virus; Argentina
Fil: Campos, Rodolfo Hector. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Virología; Argentina
Fil: Liotta, Domingo Javier. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina
description BACKGROUND: Human papillomavirus type 16 (HPV16) plays a central role in the development of cervical cancer. Worldwide studies indicate the existence of HPV16 variants that show different geographic distributions and oncogenic potential. OBJECTIVE: Our goal was to describe the genetic variation of HPV16 isolates identified in urban women with different grades of cervical lesions living in northeastern Argentina. STUDY DESIGN: We analyzed 116 HPV16-positive cervical samples (16 NLIM, 62 L-SIL, 16 H-SIL and 22 cervical cancer) from patients attending health centers in Misiones (Argentina) during 2006-13. HPV16 isolates were genetically characterized through PCR amplification and direct sequencing of 364 bp within the long control region, and the resulting sequences classified into variants based on phylogenetic analysis (lineages A, B, C and D). A potential association between HPV16 variants and lesion grade was evaluated through an odds ratio (OR) test. A temporal framework for the origin of HPV16 variants was assessed through coalescence analysis (BEAST v 1.7.5). RESULTS: Phylogenetic analysis of HPV16 sequences showed that 92.1% of the samples clustered with lineage A, and 6.9% to lineage D. HPV16 variants from lineage D were more frequently associated with high-grade lesions and cancer (HSIL+) than lineage A variants at an OR of 13.8 (1.6-117.0). The time to most common recent ancestor (tMCRA) of all variants was 119,103 years before present (HPD 95%=48,486-197,239), a date consistent with the time frame for modern human evolution. CONCLUSION: Our results suggest that HPV16 variants from lineage D may represent an additional risk factor for the development of cervical cancer in women living in northeastern Argentina. This study provides new information about viral isolates present in Argentina that will contribute to the monitoring of HPV16 infection in the vaccine era.
publishDate 2015
dc.date.none.fl_str_mv 2015-01
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info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/29964
Badano, Ines; Totaro, María Elina; Culasso, Andrés Carlos Alberto; Sanabria, Daiana Jimena; Schurr, Theodore; et al.; Genetic characterization and clinical implications of human papillomavirus type 16 (HPV16) variants from northeastern Argentina.; Elsevier Science; Infection, Genetics and Evolution; 29; 1-2015; 103-109
1567-1348
1567-7257
CONICET Digital
CONICET
url http://hdl.handle.net/11336/29964
identifier_str_mv Badano, Ines; Totaro, María Elina; Culasso, Andrés Carlos Alberto; Sanabria, Daiana Jimena; Schurr, Theodore; et al.; Genetic characterization and clinical implications of human papillomavirus type 16 (HPV16) variants from northeastern Argentina.; Elsevier Science; Infection, Genetics and Evolution; 29; 1-2015; 103-109
1567-1348
1567-7257
CONICET Digital
CONICET
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dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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