Microbiota unbalance in relation to high-risk human papillomavirus cervical infection

Autores
Gomez Cherey, Juan Facundo; Payalef, Sandra Noemi; Fleider, Laura; Reyes, Ana Paula; Maldonado, Verónica Andrea; Losada, Mirta Olga; Chen, Xin; Cardinal, Lucía Helena; Wang, Youxiang; Tatti, Silvio Alejandro; Perazzi, Beatriz Elizabeth
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective To assess vaginal dysfunction using basic vaginal states and the presence of lactobacillary microbiota in patients with HPV infection with no squamous intraepithelial lesions (SIL), low-grade squamous intraepithelial lesion (L-SIL) and high-grade squamous intraepithelial lesion (H-SIL) or squamous cell carcinoma compared to a control group (HPV-negative). To establish the prevalence of bacterial vaginosis, candidiasis and trichomoniasis in the different age groups and to characterize the species of lactobacilli according to type of lesion.Methods Cross-sectional study of patients who underwent clinical examination and collection of vaginal fornixes to study basic vaginal states and culture. Species identification of lactobacilli was performed by mass spectrometry. The Chi-square and Fisher´s test were used. p<0.05 was considered significant. High-risk viral types were determined using a multiplex real time PCR test.Results: A total of 741 patients were analyzed and divided into 3 age groups: Group 1, aged 18 to 24 years (n=138), Group 2, aged 25 to 50 years (n=456) and Group 3, aged over 50 years (n=147). All groups were further divided into HPV-negative (Control), HPV-positive without lesion, L-SIL, H-SIL/squamous cell carcinoma. The prevalence of unbalance basic vaginal states in H-SIL/squamous cell carcinoma was 72.7% (p=0.03) in Group 1, 53.1% (p=0.05) in Group 2, and no cases of unbalance were detected in Group 3. The prevalence of bacterial vaginosis in the H-SIL/squamous cell carcinoma in group 1 was 54.5% and in group 2 was 43.7%. Patients with H-SIL/squamous cell carcinoma had a prevalence of 21.4% of Lactobacillus crispatus, 42.9% of L. jensenii and 14.3% of L. iners.Conclusions: A greater unbalance of vaginal microbiota was observed in patients with squamous intraepithelial lesions (SIL), especially in H-SIL/squamous cell carcinoma. In this group, an increase of L. jensenii and L. iners compared to control was found. L. crispatus had similar prevalence to control group. It is important to characterize the lactobacilli species since the unbalance alters the vaginal microenvironment and acts as a cofactor in the persistence of HPV infection.
Fil: Gomez Cherey, Juan Facundo. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Payalef, Sandra Noemi. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: Fleider, Laura. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Reyes, Ana Paula. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: Maldonado, Verónica Andrea. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Losada, Mirta Olga. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina
Fil: Chen, Xin. No especifíca;
Fil: Cardinal, Lucía Helena. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Wang, Youxiang. No especifíca;
Fil: Tatti, Silvio Alejandro. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Perazzi, Beatriz Elizabeth. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
MICROBIOTA UNBALANCE
HIGH-RISK HPV
CERVICAL INFECTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/234389
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/234389
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Microbiota unbalance in relation to high-risk human papillomavirus cervical infectionGomez Cherey, Juan FacundoPayalef, Sandra NoemiFleider, LauraReyes, Ana PaulaMaldonado, Verónica AndreaLosada, Mirta OlgaChen, XinCardinal, Lucía HelenaWang, YouxiangTatti, Silvio AlejandroPerazzi, Beatriz ElizabethMICROBIOTA UNBALANCEHIGH-RISK HPVCERVICAL INFECTIONhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Objective To assess vaginal dysfunction using basic vaginal states and the presence of lactobacillary microbiota in patients with HPV infection with no squamous intraepithelial lesions (SIL), low-grade squamous intraepithelial lesion (L-SIL) and high-grade squamous intraepithelial lesion (H-SIL) or squamous cell carcinoma compared to a control group (HPV-negative). To establish the prevalence of bacterial vaginosis, candidiasis and trichomoniasis in the different age groups and to characterize the species of lactobacilli according to type of lesion.Methods Cross-sectional study of patients who underwent clinical examination and collection of vaginal fornixes to study basic vaginal states and culture. Species identification of lactobacilli was performed by mass spectrometry. The Chi-square and Fisher´s test were used. p<0.05 was considered significant. High-risk viral types were determined using a multiplex real time PCR test.Results: A total of 741 patients were analyzed and divided into 3 age groups: Group 1, aged 18 to 24 years (n=138), Group 2, aged 25 to 50 years (n=456) and Group 3, aged over 50 years (n=147). All groups were further divided into HPV-negative (Control), HPV-positive without lesion, L-SIL, H-SIL/squamous cell carcinoma. The prevalence of unbalance basic vaginal states in H-SIL/squamous cell carcinoma was 72.7% (p=0.03) in Group 1, 53.1% (p=0.05) in Group 2, and no cases of unbalance were detected in Group 3. The prevalence of bacterial vaginosis in the H-SIL/squamous cell carcinoma in group 1 was 54.5% and in group 2 was 43.7%. Patients with H-SIL/squamous cell carcinoma had a prevalence of 21.4% of Lactobacillus crispatus, 42.9% of L. jensenii and 14.3% of L. iners.Conclusions: A greater unbalance of vaginal microbiota was observed in patients with squamous intraepithelial lesions (SIL), especially in H-SIL/squamous cell carcinoma. In this group, an increase of L. jensenii and L. iners compared to control was found. L. crispatus had similar prevalence to control group. It is important to characterize the lactobacilli species since the unbalance alters the vaginal microenvironment and acts as a cofactor in the persistence of HPV infection.Fil: Gomez Cherey, Juan Facundo. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaFil: Payalef, Sandra Noemi. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; ArgentinaFil: Fleider, Laura. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaFil: Reyes, Ana Paula. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; ArgentinaFil: Maldonado, Verónica Andrea. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaFil: Losada, Mirta Olga. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; ArgentinaFil: Chen, Xin. No especifíca;Fil: Cardinal, Lucía Helena. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaFil: Wang, Youxiang. No especifíca;Fil: Tatti, Silvio Alejandro. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaFil: Perazzi, Beatriz Elizabeth. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaBMJ Publishing Group2023-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/234389Gomez Cherey, Juan Facundo; Payalef, Sandra Noemi; Fleider, Laura; Reyes, Ana Paula; Maldonado, Verónica Andrea; et al.; Microbiota unbalance in relation to high-risk human papillomavirus cervical infection; BMJ Publishing Group; International Journal of Gynecological Cancer; 33; 4; 4-2023; 482-4881525-1438CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://ijgc.bmj.com/content/early/2023/01/04/ijgc-2022-003760.citation-toolsinfo:eu-repo/semantics/altIdentifier/doi/10.1136/ijgc-2022-003760info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:37:26Zoai:ri.conicet.gov.ar:11336/234389instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:37:27.073CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Microbiota unbalance in relation to high-risk human papillomavirus cervical infection
title Microbiota unbalance in relation to high-risk human papillomavirus cervical infection
spellingShingle Microbiota unbalance in relation to high-risk human papillomavirus cervical infection
Gomez Cherey, Juan Facundo
MICROBIOTA UNBALANCE
HIGH-RISK HPV
CERVICAL INFECTION
title_short Microbiota unbalance in relation to high-risk human papillomavirus cervical infection
title_full Microbiota unbalance in relation to high-risk human papillomavirus cervical infection
title_fullStr Microbiota unbalance in relation to high-risk human papillomavirus cervical infection
title_full_unstemmed Microbiota unbalance in relation to high-risk human papillomavirus cervical infection
title_sort Microbiota unbalance in relation to high-risk human papillomavirus cervical infection
dc.creator.none.fl_str_mv Gomez Cherey, Juan Facundo
Payalef, Sandra Noemi
Fleider, Laura
Reyes, Ana Paula
Maldonado, Verónica Andrea
Losada, Mirta Olga
Chen, Xin
Cardinal, Lucía Helena
Wang, Youxiang
Tatti, Silvio Alejandro
Perazzi, Beatriz Elizabeth
author Gomez Cherey, Juan Facundo
author_facet Gomez Cherey, Juan Facundo
Payalef, Sandra Noemi
Fleider, Laura
Reyes, Ana Paula
Maldonado, Verónica Andrea
Losada, Mirta Olga
Chen, Xin
Cardinal, Lucía Helena
Wang, Youxiang
Tatti, Silvio Alejandro
Perazzi, Beatriz Elizabeth
author_role author
author2 Payalef, Sandra Noemi
Fleider, Laura
Reyes, Ana Paula
Maldonado, Verónica Andrea
Losada, Mirta Olga
Chen, Xin
Cardinal, Lucía Helena
Wang, Youxiang
Tatti, Silvio Alejandro
Perazzi, Beatriz Elizabeth
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv MICROBIOTA UNBALANCE
HIGH-RISK HPV
CERVICAL INFECTION
topic MICROBIOTA UNBALANCE
HIGH-RISK HPV
CERVICAL INFECTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Objective To assess vaginal dysfunction using basic vaginal states and the presence of lactobacillary microbiota in patients with HPV infection with no squamous intraepithelial lesions (SIL), low-grade squamous intraepithelial lesion (L-SIL) and high-grade squamous intraepithelial lesion (H-SIL) or squamous cell carcinoma compared to a control group (HPV-negative). To establish the prevalence of bacterial vaginosis, candidiasis and trichomoniasis in the different age groups and to characterize the species of lactobacilli according to type of lesion.Methods Cross-sectional study of patients who underwent clinical examination and collection of vaginal fornixes to study basic vaginal states and culture. Species identification of lactobacilli was performed by mass spectrometry. The Chi-square and Fisher´s test were used. p<0.05 was considered significant. High-risk viral types were determined using a multiplex real time PCR test.Results: A total of 741 patients were analyzed and divided into 3 age groups: Group 1, aged 18 to 24 years (n=138), Group 2, aged 25 to 50 years (n=456) and Group 3, aged over 50 years (n=147). All groups were further divided into HPV-negative (Control), HPV-positive without lesion, L-SIL, H-SIL/squamous cell carcinoma. The prevalence of unbalance basic vaginal states in H-SIL/squamous cell carcinoma was 72.7% (p=0.03) in Group 1, 53.1% (p=0.05) in Group 2, and no cases of unbalance were detected in Group 3. The prevalence of bacterial vaginosis in the H-SIL/squamous cell carcinoma in group 1 was 54.5% and in group 2 was 43.7%. Patients with H-SIL/squamous cell carcinoma had a prevalence of 21.4% of Lactobacillus crispatus, 42.9% of L. jensenii and 14.3% of L. iners.Conclusions: A greater unbalance of vaginal microbiota was observed in patients with squamous intraepithelial lesions (SIL), especially in H-SIL/squamous cell carcinoma. In this group, an increase of L. jensenii and L. iners compared to control was found. L. crispatus had similar prevalence to control group. It is important to characterize the lactobacilli species since the unbalance alters the vaginal microenvironment and acts as a cofactor in the persistence of HPV infection.
Fil: Gomez Cherey, Juan Facundo. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Payalef, Sandra Noemi. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: Fleider, Laura. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Reyes, Ana Paula. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: Maldonado, Verónica Andrea. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Losada, Mirta Olga. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina
Fil: Chen, Xin. No especifíca;
Fil: Cardinal, Lucía Helena. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Wang, Youxiang. No especifíca;
Fil: Tatti, Silvio Alejandro. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Perazzi, Beatriz Elizabeth. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Objective To assess vaginal dysfunction using basic vaginal states and the presence of lactobacillary microbiota in patients with HPV infection with no squamous intraepithelial lesions (SIL), low-grade squamous intraepithelial lesion (L-SIL) and high-grade squamous intraepithelial lesion (H-SIL) or squamous cell carcinoma compared to a control group (HPV-negative). To establish the prevalence of bacterial vaginosis, candidiasis and trichomoniasis in the different age groups and to characterize the species of lactobacilli according to type of lesion.Methods Cross-sectional study of patients who underwent clinical examination and collection of vaginal fornixes to study basic vaginal states and culture. Species identification of lactobacilli was performed by mass spectrometry. The Chi-square and Fisher´s test were used. p<0.05 was considered significant. High-risk viral types were determined using a multiplex real time PCR test.Results: A total of 741 patients were analyzed and divided into 3 age groups: Group 1, aged 18 to 24 years (n=138), Group 2, aged 25 to 50 years (n=456) and Group 3, aged over 50 years (n=147). All groups were further divided into HPV-negative (Control), HPV-positive without lesion, L-SIL, H-SIL/squamous cell carcinoma. The prevalence of unbalance basic vaginal states in H-SIL/squamous cell carcinoma was 72.7% (p=0.03) in Group 1, 53.1% (p=0.05) in Group 2, and no cases of unbalance were detected in Group 3. The prevalence of bacterial vaginosis in the H-SIL/squamous cell carcinoma in group 1 was 54.5% and in group 2 was 43.7%. Patients with H-SIL/squamous cell carcinoma had a prevalence of 21.4% of Lactobacillus crispatus, 42.9% of L. jensenii and 14.3% of L. iners.Conclusions: A greater unbalance of vaginal microbiota was observed in patients with squamous intraepithelial lesions (SIL), especially in H-SIL/squamous cell carcinoma. In this group, an increase of L. jensenii and L. iners compared to control was found. L. crispatus had similar prevalence to control group. It is important to characterize the lactobacilli species since the unbalance alters the vaginal microenvironment and acts as a cofactor in the persistence of HPV infection.
publishDate 2023
dc.date.none.fl_str_mv 2023-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/234389
Gomez Cherey, Juan Facundo; Payalef, Sandra Noemi; Fleider, Laura; Reyes, Ana Paula; Maldonado, Verónica Andrea; et al.; Microbiota unbalance in relation to high-risk human papillomavirus cervical infection; BMJ Publishing Group; International Journal of Gynecological Cancer; 33; 4; 4-2023; 482-488
1525-1438
CONICET Digital
CONICET
url http://hdl.handle.net/11336/234389
identifier_str_mv Gomez Cherey, Juan Facundo; Payalef, Sandra Noemi; Fleider, Laura; Reyes, Ana Paula; Maldonado, Verónica Andrea; et al.; Microbiota unbalance in relation to high-risk human papillomavirus cervical infection; BMJ Publishing Group; International Journal of Gynecological Cancer; 33; 4; 4-2023; 482-488
1525-1438
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://ijgc.bmj.com/content/early/2023/01/04/ijgc-2022-003760.citation-tools
info:eu-repo/semantics/altIdentifier/doi/10.1136/ijgc-2022-003760
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv BMJ Publishing Group
publisher.none.fl_str_mv BMJ Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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