Genetic Variability in the E6/E7 Region of Human Papillomavirus 16 in Women from Ecuador

Autores
Zhingre, Alicia; Bedoya Pilozo, César; Gutierrez Pallo, Diana; Badano, Ines; Herrera Yela, Andrés; Salazar, Zoila; Alarcón, Damaris; Arguello Bravo, Natali; Espinoza, Maylen; Ponce, Patricio; Soto, Yudira; Carrazco Montalvo, Andrés Ricardo
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Human Papillomavirus (HPV) infection is associated with intraepithelial neoplasia and cervical cancer (CC). Ecuador has a high prevalence of cervical cancer, with more than 1600 new cases diagnosed annually. This study aimed to analyze oncogenes E6 and E7 of HPV16 in samples collected from women with cancerous and precancerous cervical lesions from the Ecuadorian coast. Twenty-nine women, including six with ASCUS, three with LSIL, thirteen with HSIL, and seven with Cacu, were analyzed. The most common SNPs were E6 350G or L83V (82.6%) and E6 145T/286A/289G/335T/350G or Q14H/F78Y/L83V (17.4%). Both variants are reported to be associated with an increased risk of cervical cancer in worldwide studies. In contrast, all E7 genes have conserved amino-acid positions. Phylogenetic trees showed the circulation of the D (26.1%) and A (73.9) lineages. The frequency of D was higher than that reported in other comparable studies in Ecuador and Latin America, and may be related to the ethnic composition of the studied populations. This study contributes to the characterization of the potential risk factors for cervical carcinogenesis associated with Ecuadorian women infected with HPV16.
Fil: Zhingre, Alicia. Universidad Católica de Cuenca; Ecuador
Fil: Bedoya Pilozo, César. Hospital Luis Vernaza; Ecuador
Fil: Gutierrez Pallo, Diana. Instituto Nacional de Investigación en Salud Pública “Leopoldo Izquieta Pérez”; Ecuador
Fil: Badano, Ines. Universidad Nacional de Misiones; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Herrera Yela, Andrés. Pontificia Universidad Católica del Ecuador; Ecuador
Fil: Salazar, Zoila. Universidad Católica de Cuenca; Ecuador
Fil: Alarcón, Damaris. Instituto Nacional de Investigación en Salud Pública “Leopoldo Izquieta Pérez”; Ecuador
Fil: Arguello Bravo, Natali. Instituto Nacional de Investigación en Salud Pública “Leopoldo Izquieta Pérez”; Ecuador
Fil: Espinoza, Maylen. No especifíca;
Fil: Ponce, Patricio. Instituto Nacional de Investigación en Salud Pública “Leopoldo Izquieta Pérez”; Ecuador
Fil: Soto, Yudira. Instituto de Medicina Tropical “Pedro Kouri”; Cuba
Fil: Carrazco Montalvo, Andrés Ricardo. Instituto Nacional de Investigación en Salud Pública “Leopoldo Izquieta Pérez”; Ecuador. Universidad Autónoma de Madrid; España
Materia
cervical cancer
genetics
Human papillomavirus
Lineage
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/235433
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/235433
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genetic Variability in the E6/E7 Region of Human Papillomavirus 16 in Women from EcuadorZhingre, AliciaBedoya Pilozo, CésarGutierrez Pallo, DianaBadano, InesHerrera Yela, AndrésSalazar, ZoilaAlarcón, DamarisArguello Bravo, NataliEspinoza, MaylenPonce, PatricioSoto, YudiraCarrazco Montalvo, Andrés Ricardocervical cancergeneticsHuman papillomavirusLineagehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Human Papillomavirus (HPV) infection is associated with intraepithelial neoplasia and cervical cancer (CC). Ecuador has a high prevalence of cervical cancer, with more than 1600 new cases diagnosed annually. This study aimed to analyze oncogenes E6 and E7 of HPV16 in samples collected from women with cancerous and precancerous cervical lesions from the Ecuadorian coast. Twenty-nine women, including six with ASCUS, three with LSIL, thirteen with HSIL, and seven with Cacu, were analyzed. The most common SNPs were E6 350G or L83V (82.6%) and E6 145T/286A/289G/335T/350G or Q14H/F78Y/L83V (17.4%). Both variants are reported to be associated with an increased risk of cervical cancer in worldwide studies. In contrast, all E7 genes have conserved amino-acid positions. Phylogenetic trees showed the circulation of the D (26.1%) and A (73.9) lineages. The frequency of D was higher than that reported in other comparable studies in Ecuador and Latin America, and may be related to the ethnic composition of the studied populations. This study contributes to the characterization of the potential risk factors for cervical carcinogenesis associated with Ecuadorian women infected with HPV16.Fil: Zhingre, Alicia. Universidad Católica de Cuenca; EcuadorFil: Bedoya Pilozo, César. Hospital Luis Vernaza; EcuadorFil: Gutierrez Pallo, Diana. Instituto Nacional de Investigación en Salud Pública “Leopoldo Izquieta Pérez”; EcuadorFil: Badano, Ines. Universidad Nacional de Misiones; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Herrera Yela, Andrés. Pontificia Universidad Católica del Ecuador; EcuadorFil: Salazar, Zoila. Universidad Católica de Cuenca; EcuadorFil: Alarcón, Damaris. Instituto Nacional de Investigación en Salud Pública “Leopoldo Izquieta Pérez”; EcuadorFil: Arguello Bravo, Natali. Instituto Nacional de Investigación en Salud Pública “Leopoldo Izquieta Pérez”; EcuadorFil: Espinoza, Maylen. No especifíca;Fil: Ponce, Patricio. Instituto Nacional de Investigación en Salud Pública “Leopoldo Izquieta Pérez”; EcuadorFil: Soto, Yudira. Instituto de Medicina Tropical “Pedro Kouri”; CubaFil: Carrazco Montalvo, Andrés Ricardo. Instituto Nacional de Investigación en Salud Pública “Leopoldo Izquieta Pérez”; Ecuador. Universidad Autónoma de Madrid; EspañaMDPI2023-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/235433Zhingre, Alicia; Bedoya Pilozo, César; Gutierrez Pallo, Diana; Badano, Ines; Herrera Yela, Andrés; et al.; Genetic Variability in the E6/E7 Region of Human Papillomavirus 16 in Women from Ecuador; MDPI; Viruses; 15; 6; 6-2023; 1-91999-4915CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/v15061393info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:53:51Zoai:ri.conicet.gov.ar:11336/235433instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:53:52.301CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genetic Variability in the E6/E7 Region of Human Papillomavirus 16 in Women from Ecuador
title Genetic Variability in the E6/E7 Region of Human Papillomavirus 16 in Women from Ecuador
spellingShingle Genetic Variability in the E6/E7 Region of Human Papillomavirus 16 in Women from Ecuador
Zhingre, Alicia
cervical cancer
genetics
Human papillomavirus
Lineage
title_short Genetic Variability in the E6/E7 Region of Human Papillomavirus 16 in Women from Ecuador
title_full Genetic Variability in the E6/E7 Region of Human Papillomavirus 16 in Women from Ecuador
title_fullStr Genetic Variability in the E6/E7 Region of Human Papillomavirus 16 in Women from Ecuador
title_full_unstemmed Genetic Variability in the E6/E7 Region of Human Papillomavirus 16 in Women from Ecuador
title_sort Genetic Variability in the E6/E7 Region of Human Papillomavirus 16 in Women from Ecuador
dc.creator.none.fl_str_mv Zhingre, Alicia
Bedoya Pilozo, César
Gutierrez Pallo, Diana
Badano, Ines
Herrera Yela, Andrés
Salazar, Zoila
Alarcón, Damaris
Arguello Bravo, Natali
Espinoza, Maylen
Ponce, Patricio
Soto, Yudira
Carrazco Montalvo, Andrés Ricardo
author Zhingre, Alicia
author_facet Zhingre, Alicia
Bedoya Pilozo, César
Gutierrez Pallo, Diana
Badano, Ines
Herrera Yela, Andrés
Salazar, Zoila
Alarcón, Damaris
Arguello Bravo, Natali
Espinoza, Maylen
Ponce, Patricio
Soto, Yudira
Carrazco Montalvo, Andrés Ricardo
author_role author
author2 Bedoya Pilozo, César
Gutierrez Pallo, Diana
Badano, Ines
Herrera Yela, Andrés
Salazar, Zoila
Alarcón, Damaris
Arguello Bravo, Natali
Espinoza, Maylen
Ponce, Patricio
Soto, Yudira
Carrazco Montalvo, Andrés Ricardo
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv cervical cancer
genetics
Human papillomavirus
Lineage
topic cervical cancer
genetics
Human papillomavirus
Lineage
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Human Papillomavirus (HPV) infection is associated with intraepithelial neoplasia and cervical cancer (CC). Ecuador has a high prevalence of cervical cancer, with more than 1600 new cases diagnosed annually. This study aimed to analyze oncogenes E6 and E7 of HPV16 in samples collected from women with cancerous and precancerous cervical lesions from the Ecuadorian coast. Twenty-nine women, including six with ASCUS, three with LSIL, thirteen with HSIL, and seven with Cacu, were analyzed. The most common SNPs were E6 350G or L83V (82.6%) and E6 145T/286A/289G/335T/350G or Q14H/F78Y/L83V (17.4%). Both variants are reported to be associated with an increased risk of cervical cancer in worldwide studies. In contrast, all E7 genes have conserved amino-acid positions. Phylogenetic trees showed the circulation of the D (26.1%) and A (73.9) lineages. The frequency of D was higher than that reported in other comparable studies in Ecuador and Latin America, and may be related to the ethnic composition of the studied populations. This study contributes to the characterization of the potential risk factors for cervical carcinogenesis associated with Ecuadorian women infected with HPV16.
Fil: Zhingre, Alicia. Universidad Católica de Cuenca; Ecuador
Fil: Bedoya Pilozo, César. Hospital Luis Vernaza; Ecuador
Fil: Gutierrez Pallo, Diana. Instituto Nacional de Investigación en Salud Pública “Leopoldo Izquieta Pérez”; Ecuador
Fil: Badano, Ines. Universidad Nacional de Misiones; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Herrera Yela, Andrés. Pontificia Universidad Católica del Ecuador; Ecuador
Fil: Salazar, Zoila. Universidad Católica de Cuenca; Ecuador
Fil: Alarcón, Damaris. Instituto Nacional de Investigación en Salud Pública “Leopoldo Izquieta Pérez”; Ecuador
Fil: Arguello Bravo, Natali. Instituto Nacional de Investigación en Salud Pública “Leopoldo Izquieta Pérez”; Ecuador
Fil: Espinoza, Maylen. No especifíca;
Fil: Ponce, Patricio. Instituto Nacional de Investigación en Salud Pública “Leopoldo Izquieta Pérez”; Ecuador
Fil: Soto, Yudira. Instituto de Medicina Tropical “Pedro Kouri”; Cuba
Fil: Carrazco Montalvo, Andrés Ricardo. Instituto Nacional de Investigación en Salud Pública “Leopoldo Izquieta Pérez”; Ecuador. Universidad Autónoma de Madrid; España
description Human Papillomavirus (HPV) infection is associated with intraepithelial neoplasia and cervical cancer (CC). Ecuador has a high prevalence of cervical cancer, with more than 1600 new cases diagnosed annually. This study aimed to analyze oncogenes E6 and E7 of HPV16 in samples collected from women with cancerous and precancerous cervical lesions from the Ecuadorian coast. Twenty-nine women, including six with ASCUS, three with LSIL, thirteen with HSIL, and seven with Cacu, were analyzed. The most common SNPs were E6 350G or L83V (82.6%) and E6 145T/286A/289G/335T/350G or Q14H/F78Y/L83V (17.4%). Both variants are reported to be associated with an increased risk of cervical cancer in worldwide studies. In contrast, all E7 genes have conserved amino-acid positions. Phylogenetic trees showed the circulation of the D (26.1%) and A (73.9) lineages. The frequency of D was higher than that reported in other comparable studies in Ecuador and Latin America, and may be related to the ethnic composition of the studied populations. This study contributes to the characterization of the potential risk factors for cervical carcinogenesis associated with Ecuadorian women infected with HPV16.
publishDate 2023
dc.date.none.fl_str_mv 2023-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/235433
Zhingre, Alicia; Bedoya Pilozo, César; Gutierrez Pallo, Diana; Badano, Ines; Herrera Yela, Andrés; et al.; Genetic Variability in the E6/E7 Region of Human Papillomavirus 16 in Women from Ecuador; MDPI; Viruses; 15; 6; 6-2023; 1-9
1999-4915
CONICET Digital
CONICET
url http://hdl.handle.net/11336/235433
identifier_str_mv Zhingre, Alicia; Bedoya Pilozo, César; Gutierrez Pallo, Diana; Badano, Ines; Herrera Yela, Andrés; et al.; Genetic Variability in the E6/E7 Region of Human Papillomavirus 16 in Women from Ecuador; MDPI; Viruses; 15; 6; 6-2023; 1-9
1999-4915
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/v15061393
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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