Composite Actinorhizal Plants with Transgenic Roots for the Study of Symbiotic Associations with Frankia

Autores
Meriem Benabdoun, Faiza; Nambiar Veetil, Mathish; Imanishi, Leandro Ezequiel; Svistoonoff, Sergio; Ykhlef, Nadia; Gherbi, Hassen; Franche, Claudine
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
More than 200 species of dicotyledonous plants belonging to eight different families and 24 genera can establish actinorhizal symbiosis with the nitrogen-fixing soil actinomycete Frankia. Compared to the symbiotic interaction between legumes and rhizobia, little is known about the molecular basis of the infection process and nodule formation in actinorhizal plants. Here, we review a gene transfer system based on Agrobacterium rhizogenes that opens the possibility to rapidly analyze the function of candidate symbiotic genes. The transformation protocol generates ?composite plants? that consist of a nontransgenic aerial part with transformed hairy roots. Composite plants have already been obtained in three different species of actinorhizal plants, including the tropical tree species Casuarina glauca, the Patagonian shrub Discaria trinervis, and the nonwoody plant Datisca glomerata. The potential of this technique to advancing our understanding of the molecular mechanisms underlying infection by Frankia is demonstrated by functional analyses of symbiotic genes.
Fil: Meriem Benabdoun, Faiza. Université Mentouri; Argelia
Fil: Nambiar Veetil, Mathish. No especifíca;
Fil: Imanishi, Leandro Ezequiel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Svistoonoff, Sergio. No especifíca;
Fil: Ykhlef, Nadia. Université Mentouri; Argelia
Fil: Gherbi, Hassen. No especifíca;
Fil: Franche, Claudine. No especifíca;
Materia
BIOLOGICAL NITROGEN FIXATION
ACTINORHIZAL PLANTS
FRANKIA
AGROBACTERIUM RHIZOGENES
COMPOSITE PLANTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/192957
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Composite Actinorhizal Plants with Transgenic Roots for the Study of Symbiotic Associations with FrankiaMeriem Benabdoun, FaizaNambiar Veetil, MathishImanishi, Leandro EzequielSvistoonoff, SergioYkhlef, NadiaGherbi, HassenFranche, ClaudineBIOLOGICAL NITROGEN FIXATIONACTINORHIZAL PLANTSFRANKIAAGROBACTERIUM RHIZOGENESCOMPOSITE PLANTShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1More than 200 species of dicotyledonous plants belonging to eight different families and 24 genera can establish actinorhizal symbiosis with the nitrogen-fixing soil actinomycete Frankia. Compared to the symbiotic interaction between legumes and rhizobia, little is known about the molecular basis of the infection process and nodule formation in actinorhizal plants. Here, we review a gene transfer system based on Agrobacterium rhizogenes that opens the possibility to rapidly analyze the function of candidate symbiotic genes. The transformation protocol generates ?composite plants? that consist of a nontransgenic aerial part with transformed hairy roots. Composite plants have already been obtained in three different species of actinorhizal plants, including the tropical tree species Casuarina glauca, the Patagonian shrub Discaria trinervis, and the nonwoody plant Datisca glomerata. The potential of this technique to advancing our understanding of the molecular mechanisms underlying infection by Frankia is demonstrated by functional analyses of symbiotic genes.Fil: Meriem Benabdoun, Faiza. Université Mentouri; ArgeliaFil: Nambiar Veetil, Mathish. No especifíca;Fil: Imanishi, Leandro Ezequiel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Svistoonoff, Sergio. No especifíca;Fil: Ykhlef, Nadia. Université Mentouri; ArgeliaFil: Gherbi, Hassen. No especifíca;Fil: Franche, Claudine. No especifíca;Hindawi Publishing Corporation2011-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/192957Meriem Benabdoun, Faiza; Nambiar Veetil, Mathish; Imanishi, Leandro Ezequiel; Svistoonoff, Sergio; Ykhlef, Nadia; et al.; Composite Actinorhizal Plants with Transgenic Roots for the Study of Symbiotic Associations with Frankia; Hindawi Publishing Corporation; Journal of Botany; 8-2011; 1-82090-0120CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1155/2011/702947info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/jb/2011/702947/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:44:22Zoai:ri.conicet.gov.ar:11336/192957instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:44:22.555CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Composite Actinorhizal Plants with Transgenic Roots for the Study of Symbiotic Associations with Frankia
title Composite Actinorhizal Plants with Transgenic Roots for the Study of Symbiotic Associations with Frankia
spellingShingle Composite Actinorhizal Plants with Transgenic Roots for the Study of Symbiotic Associations with Frankia
Meriem Benabdoun, Faiza
BIOLOGICAL NITROGEN FIXATION
ACTINORHIZAL PLANTS
FRANKIA
AGROBACTERIUM RHIZOGENES
COMPOSITE PLANTS
title_short Composite Actinorhizal Plants with Transgenic Roots for the Study of Symbiotic Associations with Frankia
title_full Composite Actinorhizal Plants with Transgenic Roots for the Study of Symbiotic Associations with Frankia
title_fullStr Composite Actinorhizal Plants with Transgenic Roots for the Study of Symbiotic Associations with Frankia
title_full_unstemmed Composite Actinorhizal Plants with Transgenic Roots for the Study of Symbiotic Associations with Frankia
title_sort Composite Actinorhizal Plants with Transgenic Roots for the Study of Symbiotic Associations with Frankia
dc.creator.none.fl_str_mv Meriem Benabdoun, Faiza
Nambiar Veetil, Mathish
Imanishi, Leandro Ezequiel
Svistoonoff, Sergio
Ykhlef, Nadia
Gherbi, Hassen
Franche, Claudine
author Meriem Benabdoun, Faiza
author_facet Meriem Benabdoun, Faiza
Nambiar Veetil, Mathish
Imanishi, Leandro Ezequiel
Svistoonoff, Sergio
Ykhlef, Nadia
Gherbi, Hassen
Franche, Claudine
author_role author
author2 Nambiar Veetil, Mathish
Imanishi, Leandro Ezequiel
Svistoonoff, Sergio
Ykhlef, Nadia
Gherbi, Hassen
Franche, Claudine
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv BIOLOGICAL NITROGEN FIXATION
ACTINORHIZAL PLANTS
FRANKIA
AGROBACTERIUM RHIZOGENES
COMPOSITE PLANTS
topic BIOLOGICAL NITROGEN FIXATION
ACTINORHIZAL PLANTS
FRANKIA
AGROBACTERIUM RHIZOGENES
COMPOSITE PLANTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv More than 200 species of dicotyledonous plants belonging to eight different families and 24 genera can establish actinorhizal symbiosis with the nitrogen-fixing soil actinomycete Frankia. Compared to the symbiotic interaction between legumes and rhizobia, little is known about the molecular basis of the infection process and nodule formation in actinorhizal plants. Here, we review a gene transfer system based on Agrobacterium rhizogenes that opens the possibility to rapidly analyze the function of candidate symbiotic genes. The transformation protocol generates ?composite plants? that consist of a nontransgenic aerial part with transformed hairy roots. Composite plants have already been obtained in three different species of actinorhizal plants, including the tropical tree species Casuarina glauca, the Patagonian shrub Discaria trinervis, and the nonwoody plant Datisca glomerata. The potential of this technique to advancing our understanding of the molecular mechanisms underlying infection by Frankia is demonstrated by functional analyses of symbiotic genes.
Fil: Meriem Benabdoun, Faiza. Université Mentouri; Argelia
Fil: Nambiar Veetil, Mathish. No especifíca;
Fil: Imanishi, Leandro Ezequiel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Svistoonoff, Sergio. No especifíca;
Fil: Ykhlef, Nadia. Université Mentouri; Argelia
Fil: Gherbi, Hassen. No especifíca;
Fil: Franche, Claudine. No especifíca;
description More than 200 species of dicotyledonous plants belonging to eight different families and 24 genera can establish actinorhizal symbiosis with the nitrogen-fixing soil actinomycete Frankia. Compared to the symbiotic interaction between legumes and rhizobia, little is known about the molecular basis of the infection process and nodule formation in actinorhizal plants. Here, we review a gene transfer system based on Agrobacterium rhizogenes that opens the possibility to rapidly analyze the function of candidate symbiotic genes. The transformation protocol generates ?composite plants? that consist of a nontransgenic aerial part with transformed hairy roots. Composite plants have already been obtained in three different species of actinorhizal plants, including the tropical tree species Casuarina glauca, the Patagonian shrub Discaria trinervis, and the nonwoody plant Datisca glomerata. The potential of this technique to advancing our understanding of the molecular mechanisms underlying infection by Frankia is demonstrated by functional analyses of symbiotic genes.
publishDate 2011
dc.date.none.fl_str_mv 2011-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/192957
Meriem Benabdoun, Faiza; Nambiar Veetil, Mathish; Imanishi, Leandro Ezequiel; Svistoonoff, Sergio; Ykhlef, Nadia; et al.; Composite Actinorhizal Plants with Transgenic Roots for the Study of Symbiotic Associations with Frankia; Hindawi Publishing Corporation; Journal of Botany; 8-2011; 1-8
2090-0120
CONICET Digital
CONICET
url http://hdl.handle.net/11336/192957
identifier_str_mv Meriem Benabdoun, Faiza; Nambiar Veetil, Mathish; Imanishi, Leandro Ezequiel; Svistoonoff, Sergio; Ykhlef, Nadia; et al.; Composite Actinorhizal Plants with Transgenic Roots for the Study of Symbiotic Associations with Frankia; Hindawi Publishing Corporation; Journal of Botany; 8-2011; 1-8
2090-0120
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1155/2011/702947
info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/jb/2011/702947/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782138041499648
score 12.982451

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