Transformation of Mecardonia (Plantaginaceae) with wild-type Agrobacterium rhizogenes efficiently improves compact growth, branching and flower related ornamental traits

Autores
Perez De La Torre, Mariana; Fernández, Paula del Carmen; Greppi, Julian Alejandro; Coviella, María Andrea; Fernández, Martín Nicolás; Astigueta, Francisco; Mata, Diego Alejandro; Trupkin, Santiago Ariel
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Mecardonia constitutes an emergent crop in the floriculture market. In order to develop plants with improved compact growth by molecular breeding, we transformed Mecardonia cv. ‘Guarani Amarilla INTA’ using a wild-type strain of Agrobacterium rhizogenes. An advantage of using this approach is that generated plants are stable and considered non-GMO in several countries. Adventitious roots were produced by inoculating Mecardonia shoots with agropine ATCC15834 strain. Twenty independent root cultures regenerated spontaneously into shoots in hormone-free medium and presence of TL-DNA oncogenes were confirmed in their genomes. We selected four root inducing (Ri)-lines, designated GME, GCA, GVI and GIN, based on their different degree of compact growth habit. Under greenhouse conditions representative Ri-lines displayed reduced internode and shoot length, shoot and root biomass, aerial plant coverage, individual leaf area, flower width, pedicel length and the number of flowers per plant; and increased the number of nodes, axillary shoots, flower density and flower width relative to aerial plant coverage and mostly normal flowering when compared to non-transformed plants. To correlate phenotypical traits with gene expression, quantitative PCR analysis was performed. Ri-line GIN showed the highest rolA-D and ORF8, ORF13-14 gene expression which correlated with its super-dwarf phenotype, whereas the most weak Ri-phenotype observed in Ri-line GME showed no presence of rolA and ORF8 genes in plant genome. Expression of rolD and ORF13 correlated with reduced aerial plant coverage, shoot weight, shoot: root ratio and increased flower density and flower width relative to plant coverage, thus being considered of particular interest in Mecardonia breeding. Expression of ORF8 and rolA correlated with reduced aerial plant coverage, pedicel length, the total number of flowers per plant and increased flower width relative to the aerial plant coverage. Moreover, ORF8 and ORF13 may have a more prominent role in plant development than previously assumed and assigned to rol-genes. Overall, a better-organized compact growth without affecting other traits could be generated.
Inst. de Floricultura
Fil: Perez De La Torre, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentina
Fil: Fernandez, Paula Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina
Fil: Greppi, Julian Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentina
Fil: Coviella, Maria Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentina
Fil: Fernández, Martín Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentina
Fil: Astigueta, Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Mata, Diego Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentina
Fil: Trupkin, Santiago Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Scientia horticulturae 234 : 300-311. (April 2018)
Materia
Plantaginaceae
Floricultura
Agrobacterium rhizogenes
Plantas Ornamentales
Floriculture
Ornamental Plants
Mecardonia
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/2175
Acceder
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oai_identifier_str oai:localhost:20.500.12123/2175
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Transformation of Mecardonia (Plantaginaceae) with wild-type Agrobacterium rhizogenes efficiently improves compact growth, branching and flower related ornamental traitsPerez De La Torre, MarianaFernández, Paula del CarmenGreppi, Julian AlejandroCoviella, María AndreaFernández, Martín NicolásAstigueta, FranciscoMata, Diego AlejandroTrupkin, Santiago ArielPlantaginaceaeFloriculturaAgrobacterium rhizogenesPlantas OrnamentalesFloricultureOrnamental PlantsMecardoniaMecardonia constitutes an emergent crop in the floriculture market. In order to develop plants with improved compact growth by molecular breeding, we transformed Mecardonia cv. ‘Guarani Amarilla INTA’ using a wild-type strain of Agrobacterium rhizogenes. An advantage of using this approach is that generated plants are stable and considered non-GMO in several countries. Adventitious roots were produced by inoculating Mecardonia shoots with agropine ATCC15834 strain. Twenty independent root cultures regenerated spontaneously into shoots in hormone-free medium and presence of TL-DNA oncogenes were confirmed in their genomes. We selected four root inducing (Ri)-lines, designated GME, GCA, GVI and GIN, based on their different degree of compact growth habit. Under greenhouse conditions representative Ri-lines displayed reduced internode and shoot length, shoot and root biomass, aerial plant coverage, individual leaf area, flower width, pedicel length and the number of flowers per plant; and increased the number of nodes, axillary shoots, flower density and flower width relative to aerial plant coverage and mostly normal flowering when compared to non-transformed plants. To correlate phenotypical traits with gene expression, quantitative PCR analysis was performed. Ri-line GIN showed the highest rolA-D and ORF8, ORF13-14 gene expression which correlated with its super-dwarf phenotype, whereas the most weak Ri-phenotype observed in Ri-line GME showed no presence of rolA and ORF8 genes in plant genome. Expression of rolD and ORF13 correlated with reduced aerial plant coverage, shoot weight, shoot: root ratio and increased flower density and flower width relative to plant coverage, thus being considered of particular interest in Mecardonia breeding. Expression of ORF8 and rolA correlated with reduced aerial plant coverage, pedicel length, the total number of flowers per plant and increased flower width relative to the aerial plant coverage. Moreover, ORF8 and ORF13 may have a more prominent role in plant development than previously assumed and assigned to rol-genes. Overall, a better-organized compact growth without affecting other traits could be generated.Inst. de FloriculturaFil: Perez De La Torre, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; ArgentinaFil: Fernandez, Paula Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; ArgentinaFil: Greppi, Julian Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; ArgentinaFil: Coviella, Maria Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; ArgentinaFil: Fernández, Martín Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; ArgentinaFil: Astigueta, Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Mata, Diego Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; ArgentinaFil: Trupkin, Santiago Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina2018-04-05T13:43:41Z2018-04-05T13:43:41Z2018-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/2175https://www.sciencedirect.com/science/article/pii/S03044238183012250304-4238https://doi.org/10.1016/j.scienta.2018.02.047Scientia horticulturae 234 : 300-311. (April 2018)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:11Zoai:localhost:20.500.12123/2175instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:12.057INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Transformation of Mecardonia (Plantaginaceae) with wild-type Agrobacterium rhizogenes efficiently improves compact growth, branching and flower related ornamental traits
title Transformation of Mecardonia (Plantaginaceae) with wild-type Agrobacterium rhizogenes efficiently improves compact growth, branching and flower related ornamental traits
spellingShingle Transformation of Mecardonia (Plantaginaceae) with wild-type Agrobacterium rhizogenes efficiently improves compact growth, branching and flower related ornamental traits
Perez De La Torre, Mariana
Plantaginaceae
Floricultura
Agrobacterium rhizogenes
Plantas Ornamentales
Floriculture
Ornamental Plants
Mecardonia
title_short Transformation of Mecardonia (Plantaginaceae) with wild-type Agrobacterium rhizogenes efficiently improves compact growth, branching and flower related ornamental traits
title_full Transformation of Mecardonia (Plantaginaceae) with wild-type Agrobacterium rhizogenes efficiently improves compact growth, branching and flower related ornamental traits
title_fullStr Transformation of Mecardonia (Plantaginaceae) with wild-type Agrobacterium rhizogenes efficiently improves compact growth, branching and flower related ornamental traits
title_full_unstemmed Transformation of Mecardonia (Plantaginaceae) with wild-type Agrobacterium rhizogenes efficiently improves compact growth, branching and flower related ornamental traits
title_sort Transformation of Mecardonia (Plantaginaceae) with wild-type Agrobacterium rhizogenes efficiently improves compact growth, branching and flower related ornamental traits
dc.creator.none.fl_str_mv Perez De La Torre, Mariana
Fernández, Paula del Carmen
Greppi, Julian Alejandro
Coviella, María Andrea
Fernández, Martín Nicolás
Astigueta, Francisco
Mata, Diego Alejandro
Trupkin, Santiago Ariel
author Perez De La Torre, Mariana
author_facet Perez De La Torre, Mariana
Fernández, Paula del Carmen
Greppi, Julian Alejandro
Coviella, María Andrea
Fernández, Martín Nicolás
Astigueta, Francisco
Mata, Diego Alejandro
Trupkin, Santiago Ariel
author_role author
author2 Fernández, Paula del Carmen
Greppi, Julian Alejandro
Coviella, María Andrea
Fernández, Martín Nicolás
Astigueta, Francisco
Mata, Diego Alejandro
Trupkin, Santiago Ariel
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Plantaginaceae
Floricultura
Agrobacterium rhizogenes
Plantas Ornamentales
Floriculture
Ornamental Plants
Mecardonia
topic Plantaginaceae
Floricultura
Agrobacterium rhizogenes
Plantas Ornamentales
Floriculture
Ornamental Plants
Mecardonia
dc.description.none.fl_txt_mv Mecardonia constitutes an emergent crop in the floriculture market. In order to develop plants with improved compact growth by molecular breeding, we transformed Mecardonia cv. ‘Guarani Amarilla INTA’ using a wild-type strain of Agrobacterium rhizogenes. An advantage of using this approach is that generated plants are stable and considered non-GMO in several countries. Adventitious roots were produced by inoculating Mecardonia shoots with agropine ATCC15834 strain. Twenty independent root cultures regenerated spontaneously into shoots in hormone-free medium and presence of TL-DNA oncogenes were confirmed in their genomes. We selected four root inducing (Ri)-lines, designated GME, GCA, GVI and GIN, based on their different degree of compact growth habit. Under greenhouse conditions representative Ri-lines displayed reduced internode and shoot length, shoot and root biomass, aerial plant coverage, individual leaf area, flower width, pedicel length and the number of flowers per plant; and increased the number of nodes, axillary shoots, flower density and flower width relative to aerial plant coverage and mostly normal flowering when compared to non-transformed plants. To correlate phenotypical traits with gene expression, quantitative PCR analysis was performed. Ri-line GIN showed the highest rolA-D and ORF8, ORF13-14 gene expression which correlated with its super-dwarf phenotype, whereas the most weak Ri-phenotype observed in Ri-line GME showed no presence of rolA and ORF8 genes in plant genome. Expression of rolD and ORF13 correlated with reduced aerial plant coverage, shoot weight, shoot: root ratio and increased flower density and flower width relative to plant coverage, thus being considered of particular interest in Mecardonia breeding. Expression of ORF8 and rolA correlated with reduced aerial plant coverage, pedicel length, the total number of flowers per plant and increased flower width relative to the aerial plant coverage. Moreover, ORF8 and ORF13 may have a more prominent role in plant development than previously assumed and assigned to rol-genes. Overall, a better-organized compact growth without affecting other traits could be generated.
Inst. de Floricultura
Fil: Perez De La Torre, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentina
Fil: Fernandez, Paula Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina
Fil: Greppi, Julian Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentina
Fil: Coviella, Maria Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentina
Fil: Fernández, Martín Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentina
Fil: Astigueta, Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Mata, Diego Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentina
Fil: Trupkin, Santiago Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Mecardonia constitutes an emergent crop in the floriculture market. In order to develop plants with improved compact growth by molecular breeding, we transformed Mecardonia cv. ‘Guarani Amarilla INTA’ using a wild-type strain of Agrobacterium rhizogenes. An advantage of using this approach is that generated plants are stable and considered non-GMO in several countries. Adventitious roots were produced by inoculating Mecardonia shoots with agropine ATCC15834 strain. Twenty independent root cultures regenerated spontaneously into shoots in hormone-free medium and presence of TL-DNA oncogenes were confirmed in their genomes. We selected four root inducing (Ri)-lines, designated GME, GCA, GVI and GIN, based on their different degree of compact growth habit. Under greenhouse conditions representative Ri-lines displayed reduced internode and shoot length, shoot and root biomass, aerial plant coverage, individual leaf area, flower width, pedicel length and the number of flowers per plant; and increased the number of nodes, axillary shoots, flower density and flower width relative to aerial plant coverage and mostly normal flowering when compared to non-transformed plants. To correlate phenotypical traits with gene expression, quantitative PCR analysis was performed. Ri-line GIN showed the highest rolA-D and ORF8, ORF13-14 gene expression which correlated with its super-dwarf phenotype, whereas the most weak Ri-phenotype observed in Ri-line GME showed no presence of rolA and ORF8 genes in plant genome. Expression of rolD and ORF13 correlated with reduced aerial plant coverage, shoot weight, shoot: root ratio and increased flower density and flower width relative to plant coverage, thus being considered of particular interest in Mecardonia breeding. Expression of ORF8 and rolA correlated with reduced aerial plant coverage, pedicel length, the total number of flowers per plant and increased flower width relative to the aerial plant coverage. Moreover, ORF8 and ORF13 may have a more prominent role in plant development than previously assumed and assigned to rol-genes. Overall, a better-organized compact growth without affecting other traits could be generated.
publishDate 2018
dc.date.none.fl_str_mv 2018-04-05T13:43:41Z
2018-04-05T13:43:41Z
2018-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/2175
https://www.sciencedirect.com/science/article/pii/S0304423818301225
0304-4238
https://doi.org/10.1016/j.scienta.2018.02.047
url http://hdl.handle.net/20.500.12123/2175
https://www.sciencedirect.com/science/article/pii/S0304423818301225
https://doi.org/10.1016/j.scienta.2018.02.047
identifier_str_mv 0304-4238
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Scientia horticulturae 234 : 300-311. (April 2018)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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