Transformed Hairy Roots of Discaria trinervis: A Valuable Tool for Studying Actinorhizal Symbiosis in the Context of Intercellular Infection

Autores
Imanishi, Leandro Ezequiel; Vayssières, Alice; Franche, Claudine; Bogusz, Didier; Wall, Luis Gabriel; Svistoonoff, Sergio
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Among infection mechanisms leading to root nodule symbiosis, the intercellular infection pathway is probably the most ancestral but also one of the least characterized. Intercellular infection has been described in Discaria trinervis, an actinorhizal plant belonging to the Rosales order. To decipher the molecular mechanisms underlying intercellular infection with Frankia bacteria, we set up an efficient genetic transformation protocol for D. trinervis based on Agrobacterium rhizogenes. We showed that composite plants with transgenic roots expressing green fluorescent protein can be specifically and efficiently nodulated by Frankia strain BCU110501. Nitrogen fixation rates and feedback inhibition of nodule formation by nitrogen were similar in control and composite plants. In order to challenge the transformation system, the MtEnod11 promoter, a gene from Medicago truncatula widely used as a marker for early infection-related symbiotic events in model legumes, was introduced in D. trinervis. MtEnod11::GUS expression was related to infection zones in root cortex and in the parenchyma of the developing nodule. The ability to study intercellular infection with molecular tools opens new avenues for understanding the evolution of the infection process in nitrogen-fixing root nodule symbioses.
Fil: Imanishi, Leandro Ezequiel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vayssières, Alice. Institut de Recherche Pour Le Developpement; Francia
Fil: Franche, Claudine. Institut de Recherche Pour Le Developpement; Francia
Fil: Bogusz, Didier. Institut de Recherche Pour Le Developpement; Francia
Fil: Wall, Luis Gabriel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Svistoonoff, Sergio. Institut de Recherche Pour Le Developpement; Francia
Materia
ACTINORHIZAL SYMBIOSES
DISCARIA TRINERVIS
AGROBACTERIUM RHIZOGENES
NITROGEN FIXATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/193806
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/193806
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Transformed Hairy Roots of Discaria trinervis: A Valuable Tool for Studying Actinorhizal Symbiosis in the Context of Intercellular InfectionImanishi, Leandro EzequielVayssières, AliceFranche, ClaudineBogusz, DidierWall, Luis GabrielSvistoonoff, SergioACTINORHIZAL SYMBIOSESDISCARIA TRINERVISAGROBACTERIUM RHIZOGENESNITROGEN FIXATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Among infection mechanisms leading to root nodule symbiosis, the intercellular infection pathway is probably the most ancestral but also one of the least characterized. Intercellular infection has been described in Discaria trinervis, an actinorhizal plant belonging to the Rosales order. To decipher the molecular mechanisms underlying intercellular infection with Frankia bacteria, we set up an efficient genetic transformation protocol for D. trinervis based on Agrobacterium rhizogenes. We showed that composite plants with transgenic roots expressing green fluorescent protein can be specifically and efficiently nodulated by Frankia strain BCU110501. Nitrogen fixation rates and feedback inhibition of nodule formation by nitrogen were similar in control and composite plants. In order to challenge the transformation system, the MtEnod11 promoter, a gene from Medicago truncatula widely used as a marker for early infection-related symbiotic events in model legumes, was introduced in D. trinervis. MtEnod11::GUS expression was related to infection zones in root cortex and in the parenchyma of the developing nodule. The ability to study intercellular infection with molecular tools opens new avenues for understanding the evolution of the infection process in nitrogen-fixing root nodule symbioses.Fil: Imanishi, Leandro Ezequiel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vayssières, Alice. Institut de Recherche Pour Le Developpement; FranciaFil: Franche, Claudine. Institut de Recherche Pour Le Developpement; FranciaFil: Bogusz, Didier. Institut de Recherche Pour Le Developpement; FranciaFil: Wall, Luis Gabriel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Svistoonoff, Sergio. Institut de Recherche Pour Le Developpement; FranciaAmerican Phytopathological Society2011-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/193806Imanishi, Leandro Ezequiel; Vayssières, Alice; Franche, Claudine; Bogusz, Didier; Wall, Luis Gabriel; et al.; Transformed Hairy Roots of Discaria trinervis: A Valuable Tool for Studying Actinorhizal Symbiosis in the Context of Intercellular Infection; American Phytopathological Society; Molecular Plant-Microbe Interactions; 24; 11; 11-2011; 1317-13240894-0282CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1094/MPMI-03-11-0078info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:47:30Zoai:ri.conicet.gov.ar:11336/193806instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:47:30.486CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Transformed Hairy Roots of Discaria trinervis: A Valuable Tool for Studying Actinorhizal Symbiosis in the Context of Intercellular Infection
title Transformed Hairy Roots of Discaria trinervis: A Valuable Tool for Studying Actinorhizal Symbiosis in the Context of Intercellular Infection
spellingShingle Transformed Hairy Roots of Discaria trinervis: A Valuable Tool for Studying Actinorhizal Symbiosis in the Context of Intercellular Infection
Imanishi, Leandro Ezequiel
ACTINORHIZAL SYMBIOSES
DISCARIA TRINERVIS
AGROBACTERIUM RHIZOGENES
NITROGEN FIXATION
title_short Transformed Hairy Roots of Discaria trinervis: A Valuable Tool for Studying Actinorhizal Symbiosis in the Context of Intercellular Infection
title_full Transformed Hairy Roots of Discaria trinervis: A Valuable Tool for Studying Actinorhizal Symbiosis in the Context of Intercellular Infection
title_fullStr Transformed Hairy Roots of Discaria trinervis: A Valuable Tool for Studying Actinorhizal Symbiosis in the Context of Intercellular Infection
title_full_unstemmed Transformed Hairy Roots of Discaria trinervis: A Valuable Tool for Studying Actinorhizal Symbiosis in the Context of Intercellular Infection
title_sort Transformed Hairy Roots of Discaria trinervis: A Valuable Tool for Studying Actinorhizal Symbiosis in the Context of Intercellular Infection
dc.creator.none.fl_str_mv Imanishi, Leandro Ezequiel
Vayssières, Alice
Franche, Claudine
Bogusz, Didier
Wall, Luis Gabriel
Svistoonoff, Sergio
author Imanishi, Leandro Ezequiel
author_facet Imanishi, Leandro Ezequiel
Vayssières, Alice
Franche, Claudine
Bogusz, Didier
Wall, Luis Gabriel
Svistoonoff, Sergio
author_role author
author2 Vayssières, Alice
Franche, Claudine
Bogusz, Didier
Wall, Luis Gabriel
Svistoonoff, Sergio
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ACTINORHIZAL SYMBIOSES
DISCARIA TRINERVIS
AGROBACTERIUM RHIZOGENES
NITROGEN FIXATION
topic ACTINORHIZAL SYMBIOSES
DISCARIA TRINERVIS
AGROBACTERIUM RHIZOGENES
NITROGEN FIXATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Among infection mechanisms leading to root nodule symbiosis, the intercellular infection pathway is probably the most ancestral but also one of the least characterized. Intercellular infection has been described in Discaria trinervis, an actinorhizal plant belonging to the Rosales order. To decipher the molecular mechanisms underlying intercellular infection with Frankia bacteria, we set up an efficient genetic transformation protocol for D. trinervis based on Agrobacterium rhizogenes. We showed that composite plants with transgenic roots expressing green fluorescent protein can be specifically and efficiently nodulated by Frankia strain BCU110501. Nitrogen fixation rates and feedback inhibition of nodule formation by nitrogen were similar in control and composite plants. In order to challenge the transformation system, the MtEnod11 promoter, a gene from Medicago truncatula widely used as a marker for early infection-related symbiotic events in model legumes, was introduced in D. trinervis. MtEnod11::GUS expression was related to infection zones in root cortex and in the parenchyma of the developing nodule. The ability to study intercellular infection with molecular tools opens new avenues for understanding the evolution of the infection process in nitrogen-fixing root nodule symbioses.
Fil: Imanishi, Leandro Ezequiel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vayssières, Alice. Institut de Recherche Pour Le Developpement; Francia
Fil: Franche, Claudine. Institut de Recherche Pour Le Developpement; Francia
Fil: Bogusz, Didier. Institut de Recherche Pour Le Developpement; Francia
Fil: Wall, Luis Gabriel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Svistoonoff, Sergio. Institut de Recherche Pour Le Developpement; Francia
description Among infection mechanisms leading to root nodule symbiosis, the intercellular infection pathway is probably the most ancestral but also one of the least characterized. Intercellular infection has been described in Discaria trinervis, an actinorhizal plant belonging to the Rosales order. To decipher the molecular mechanisms underlying intercellular infection with Frankia bacteria, we set up an efficient genetic transformation protocol for D. trinervis based on Agrobacterium rhizogenes. We showed that composite plants with transgenic roots expressing green fluorescent protein can be specifically and efficiently nodulated by Frankia strain BCU110501. Nitrogen fixation rates and feedback inhibition of nodule formation by nitrogen were similar in control and composite plants. In order to challenge the transformation system, the MtEnod11 promoter, a gene from Medicago truncatula widely used as a marker for early infection-related symbiotic events in model legumes, was introduced in D. trinervis. MtEnod11::GUS expression was related to infection zones in root cortex and in the parenchyma of the developing nodule. The ability to study intercellular infection with molecular tools opens new avenues for understanding the evolution of the infection process in nitrogen-fixing root nodule symbioses.
publishDate 2011
dc.date.none.fl_str_mv 2011-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/193806
Imanishi, Leandro Ezequiel; Vayssières, Alice; Franche, Claudine; Bogusz, Didier; Wall, Luis Gabriel; et al.; Transformed Hairy Roots of Discaria trinervis: A Valuable Tool for Studying Actinorhizal Symbiosis in the Context of Intercellular Infection; American Phytopathological Society; Molecular Plant-Microbe Interactions; 24; 11; 11-2011; 1317-1324
0894-0282
CONICET Digital
CONICET
url http://hdl.handle.net/11336/193806
identifier_str_mv Imanishi, Leandro Ezequiel; Vayssières, Alice; Franche, Claudine; Bogusz, Didier; Wall, Luis Gabriel; et al.; Transformed Hairy Roots of Discaria trinervis: A Valuable Tool for Studying Actinorhizal Symbiosis in the Context of Intercellular Infection; American Phytopathological Society; Molecular Plant-Microbe Interactions; 24; 11; 11-2011; 1317-1324
0894-0282
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1094/MPMI-03-11-0078
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Phytopathological Society
publisher.none.fl_str_mv American Phytopathological Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613479949926400
score 13.070432

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