Mutation detection of E6 and LCR genes from HPV 16 associated with carcinogenesis

Autores
Mosmann, Jessica Paola; Monetti, Marina Soledad; Frutos, Maria Celia; Kiguen, Ana Ximena; Venezuela, Raul Fernando; Cuffini, Cecilia Gabriela
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Human papillomavirus (HPV) is responsible for one of the most frequent sexually transmitted infections. The first phylogenetic analysis was based on a LCR region fragment. Nowadays, 4 variants are known: African (Af-1, Af-2), Asian-American (AA) and European (E). However the existence of sub-lineages of the European variant havs been proposed, specific mutations in the E6 and LCR sequences being possibly related to persistent viral infections. The aim of this study was a phylogenetic study of HPV16 sequences of endocervical samples from Córdoba, in order to detect the circulating lineages and analyze the presence of mutations that could be correlated with malignant disease. The phylogenetic analysis determined that 86% of the samples belonged to the E variant, 7% to AF-1 and the remaining 7% to AF-2. The most frequent mutation in LCR sequences was G7521A, in 80% of the analyzed samples; it affects the binding site of a transcription factor that could contribute to carcinogenesis. In the E6 sequences, the most common mutation was T350G (L83V), detected in 67% of the samples, associated with increased risk of persistent infection. The high detection rate of the European lineage correlated with patterns of human migration. This study emphasizes the importance of recognizing circulating lineages, as well as the detection of mutations associated with high-grade neoplastic lesions that could be correlated to the development of carcinogenic lesions.
Fil: Mosmann, Jessica Paola. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Monetti, Marina Soledad. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Frutos, Maria Celia. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Kiguen, Ana Ximena. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Venezuela, Raul Fernando. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Cuffini, Cecilia Gabriela. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Materia
Cervical Cancer
E6
Hpv 16
Lcr
Lineages
Mutations
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/59826
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/59826
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Mutation detection of E6 and LCR genes from HPV 16 associated with carcinogenesisMosmann, Jessica PaolaMonetti, Marina SoledadFrutos, Maria CeliaKiguen, Ana XimenaVenezuela, Raul FernandoCuffini, Cecilia GabrielaCervical CancerE6Hpv 16LcrLineagesMutationshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Human papillomavirus (HPV) is responsible for one of the most frequent sexually transmitted infections. The first phylogenetic analysis was based on a LCR region fragment. Nowadays, 4 variants are known: African (Af-1, Af-2), Asian-American (AA) and European (E). However the existence of sub-lineages of the European variant havs been proposed, specific mutations in the E6 and LCR sequences being possibly related to persistent viral infections. The aim of this study was a phylogenetic study of HPV16 sequences of endocervical samples from Córdoba, in order to detect the circulating lineages and analyze the presence of mutations that could be correlated with malignant disease. The phylogenetic analysis determined that 86% of the samples belonged to the E variant, 7% to AF-1 and the remaining 7% to AF-2. The most frequent mutation in LCR sequences was G7521A, in 80% of the analyzed samples; it affects the binding site of a transcription factor that could contribute to carcinogenesis. In the E6 sequences, the most common mutation was T350G (L83V), detected in 67% of the samples, associated with increased risk of persistent infection. The high detection rate of the European lineage correlated with patterns of human migration. This study emphasizes the importance of recognizing circulating lineages, as well as the detection of mutations associated with high-grade neoplastic lesions that could be correlated to the development of carcinogenic lesions.Fil: Mosmann, Jessica Paola. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; ArgentinaFil: Monetti, Marina Soledad. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; ArgentinaFil: Frutos, Maria Celia. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; ArgentinaFil: Kiguen, Ana Ximena. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; ArgentinaFil: Venezuela, Raul Fernando. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; ArgentinaFil: Cuffini, Cecilia Gabriela. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; ArgentinaAsian Pacific Organization Cancer Prevention2015-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/59826Mosmann, Jessica Paola; Monetti, Marina Soledad; Frutos, Maria Celia; Kiguen, Ana Ximena; Venezuela, Raul Fernando; et al.; Mutation detection of E6 and LCR genes from HPV 16 associated with carcinogenesis; Asian Pacific Organization Cancer Prevention; Asian Pacific Journal of Cancer Prevention; 16; 3; 3-2015; 1151-11571513-73681513-7368CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://bit.ly/2NIeJ3Jinfo:eu-repo/semantics/altIdentifier/doi/10.7314/APJCP.2015.16.3.1151info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:58Zoai:ri.conicet.gov.ar:11336/59826instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:58.62CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Mutation detection of E6 and LCR genes from HPV 16 associated with carcinogenesis
title Mutation detection of E6 and LCR genes from HPV 16 associated with carcinogenesis
spellingShingle Mutation detection of E6 and LCR genes from HPV 16 associated with carcinogenesis
Mosmann, Jessica Paola
Cervical Cancer
E6
Hpv 16
Lcr
Lineages
Mutations
title_short Mutation detection of E6 and LCR genes from HPV 16 associated with carcinogenesis
title_full Mutation detection of E6 and LCR genes from HPV 16 associated with carcinogenesis
title_fullStr Mutation detection of E6 and LCR genes from HPV 16 associated with carcinogenesis
title_full_unstemmed Mutation detection of E6 and LCR genes from HPV 16 associated with carcinogenesis
title_sort Mutation detection of E6 and LCR genes from HPV 16 associated with carcinogenesis
dc.creator.none.fl_str_mv Mosmann, Jessica Paola
Monetti, Marina Soledad
Frutos, Maria Celia
Kiguen, Ana Ximena
Venezuela, Raul Fernando
Cuffini, Cecilia Gabriela
author Mosmann, Jessica Paola
author_facet Mosmann, Jessica Paola
Monetti, Marina Soledad
Frutos, Maria Celia
Kiguen, Ana Ximena
Venezuela, Raul Fernando
Cuffini, Cecilia Gabriela
author_role author
author2 Monetti, Marina Soledad
Frutos, Maria Celia
Kiguen, Ana Ximena
Venezuela, Raul Fernando
Cuffini, Cecilia Gabriela
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Cervical Cancer
E6
Hpv 16
Lcr
Lineages
Mutations
topic Cervical Cancer
E6
Hpv 16
Lcr
Lineages
Mutations
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Human papillomavirus (HPV) is responsible for one of the most frequent sexually transmitted infections. The first phylogenetic analysis was based on a LCR region fragment. Nowadays, 4 variants are known: African (Af-1, Af-2), Asian-American (AA) and European (E). However the existence of sub-lineages of the European variant havs been proposed, specific mutations in the E6 and LCR sequences being possibly related to persistent viral infections. The aim of this study was a phylogenetic study of HPV16 sequences of endocervical samples from Córdoba, in order to detect the circulating lineages and analyze the presence of mutations that could be correlated with malignant disease. The phylogenetic analysis determined that 86% of the samples belonged to the E variant, 7% to AF-1 and the remaining 7% to AF-2. The most frequent mutation in LCR sequences was G7521A, in 80% of the analyzed samples; it affects the binding site of a transcription factor that could contribute to carcinogenesis. In the E6 sequences, the most common mutation was T350G (L83V), detected in 67% of the samples, associated with increased risk of persistent infection. The high detection rate of the European lineage correlated with patterns of human migration. This study emphasizes the importance of recognizing circulating lineages, as well as the detection of mutations associated with high-grade neoplastic lesions that could be correlated to the development of carcinogenic lesions.
Fil: Mosmann, Jessica Paola. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Monetti, Marina Soledad. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Frutos, Maria Celia. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Kiguen, Ana Ximena. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Venezuela, Raul Fernando. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Cuffini, Cecilia Gabriela. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
description Human papillomavirus (HPV) is responsible for one of the most frequent sexually transmitted infections. The first phylogenetic analysis was based on a LCR region fragment. Nowadays, 4 variants are known: African (Af-1, Af-2), Asian-American (AA) and European (E). However the existence of sub-lineages of the European variant havs been proposed, specific mutations in the E6 and LCR sequences being possibly related to persistent viral infections. The aim of this study was a phylogenetic study of HPV16 sequences of endocervical samples from Córdoba, in order to detect the circulating lineages and analyze the presence of mutations that could be correlated with malignant disease. The phylogenetic analysis determined that 86% of the samples belonged to the E variant, 7% to AF-1 and the remaining 7% to AF-2. The most frequent mutation in LCR sequences was G7521A, in 80% of the analyzed samples; it affects the binding site of a transcription factor that could contribute to carcinogenesis. In the E6 sequences, the most common mutation was T350G (L83V), detected in 67% of the samples, associated with increased risk of persistent infection. The high detection rate of the European lineage correlated with patterns of human migration. This study emphasizes the importance of recognizing circulating lineages, as well as the detection of mutations associated with high-grade neoplastic lesions that could be correlated to the development of carcinogenic lesions.
publishDate 2015
dc.date.none.fl_str_mv 2015-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/59826
Mosmann, Jessica Paola; Monetti, Marina Soledad; Frutos, Maria Celia; Kiguen, Ana Ximena; Venezuela, Raul Fernando; et al.; Mutation detection of E6 and LCR genes from HPV 16 associated with carcinogenesis; Asian Pacific Organization Cancer Prevention; Asian Pacific Journal of Cancer Prevention; 16; 3; 3-2015; 1151-1157
1513-7368
1513-7368
CONICET Digital
CONICET
url http://hdl.handle.net/11336/59826
identifier_str_mv Mosmann, Jessica Paola; Monetti, Marina Soledad; Frutos, Maria Celia; Kiguen, Ana Ximena; Venezuela, Raul Fernando; et al.; Mutation detection of E6 and LCR genes from HPV 16 associated with carcinogenesis; Asian Pacific Organization Cancer Prevention; Asian Pacific Journal of Cancer Prevention; 16; 3; 3-2015; 1151-1157
1513-7368
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://bit.ly/2NIeJ3J
info:eu-repo/semantics/altIdentifier/doi/10.7314/APJCP.2015.16.3.1151
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Asian Pacific Organization Cancer Prevention
publisher.none.fl_str_mv Asian Pacific Organization Cancer Prevention
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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