Bioguided isolation of antimicrobial polyphenols from Cuspidaria convoluta leaves and their synergistic effect with antibiotics

Autores
Torres, Carola Analía; Sturla, Mario Aníbal; Romero, Ana María; Judis, María Alicia
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective: To identify and isolate phenolic compounds from Cuspidaria convoluta, and to evaluate their antibacterial activity and synergistic effect with antibiotics. Methods: The crude extract was prepared by maceration with methanol (5%). The dry extract was suspended in water and fractionated successively. The most active extract was selected by its antibacterial activity and its total phenol content was determined by spectrophotometry and by HPLC-MS/MS. Bioactive fractions of the most active extract were separated by column chromatography and evaluated by bioautography. Isolated compounds were identified. Minimum inhibitory concentration (MIC) of these compounds was determined by microdilution broth method, and synergism with antibiotics (ampicillin, gentamicin and oxacillin) was tested by checkerboard and time-kill assays. Results: Coumaric acid, catechin/epicatechin, and luteolin were purified and identified from the extract. There was an increase in the antibacterial activity of antibiotics when they were combined with these compounds. The combination of luteolin and ampicillin had the most potent antibacterial activities. The MICs of oxacillin for each of methicillin-resistant Staphylococcus aureus strains were reduced between 4 and 8-fold when these strains were coincubated with sub-MIC (≤ ½ MIC) levels of these compounds, demonstrating that the combination had synergistic effect for all cases. Conclusions: Cuspidaria convoluta contains important pharmacologically active substances that can be used to improve antibiotic efficacy.
Fil: Torres, Carola Analía. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Sturla, Mario Aníbal. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; Argentina
Fil: Romero, Ana María. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; Argentina
Fil: Judis, María Alicia. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; Argentina
Materia
AMPICILLIN
ANTIMICROBIAL AGENTS
LUTEOLIN
MIC REDUCTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/114624
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/114624
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Bioguided isolation of antimicrobial polyphenols from Cuspidaria convoluta leaves and their synergistic effect with antibioticsTorres, Carola AnalíaSturla, Mario AníbalRomero, Ana MaríaJudis, María AliciaAMPICILLINANTIMICROBIAL AGENTSLUTEOLINMIC REDUCTIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Objective: To identify and isolate phenolic compounds from Cuspidaria convoluta, and to evaluate their antibacterial activity and synergistic effect with antibiotics. Methods: The crude extract was prepared by maceration with methanol (5%). The dry extract was suspended in water and fractionated successively. The most active extract was selected by its antibacterial activity and its total phenol content was determined by spectrophotometry and by HPLC-MS/MS. Bioactive fractions of the most active extract were separated by column chromatography and evaluated by bioautography. Isolated compounds were identified. Minimum inhibitory concentration (MIC) of these compounds was determined by microdilution broth method, and synergism with antibiotics (ampicillin, gentamicin and oxacillin) was tested by checkerboard and time-kill assays. Results: Coumaric acid, catechin/epicatechin, and luteolin were purified and identified from the extract. There was an increase in the antibacterial activity of antibiotics when they were combined with these compounds. The combination of luteolin and ampicillin had the most potent antibacterial activities. The MICs of oxacillin for each of methicillin-resistant Staphylococcus aureus strains were reduced between 4 and 8-fold when these strains were coincubated with sub-MIC (≤ ½ MIC) levels of these compounds, demonstrating that the combination had synergistic effect for all cases. Conclusions: Cuspidaria convoluta contains important pharmacologically active substances that can be used to improve antibiotic efficacy.Fil: Torres, Carola Analía. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Sturla, Mario Aníbal. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; ArgentinaFil: Romero, Ana María. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; ArgentinaFil: Judis, María Alicia. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; ArgentinaWolters Kluwer Medknow Publications2019-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/114624Torres, Carola Analía; Sturla, Mario Aníbal; Romero, Ana María; Judis, María Alicia; Bioguided isolation of antimicrobial polyphenols from Cuspidaria convoluta leaves and their synergistic effect with antibiotics; Wolters Kluwer Medknow Publications; Asian Pacific Journal of Tropical Biomedicine; 9; 10; 10-2019; 434-4422221-1691CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4103/2221-1691.269525info:eu-repo/semantics/altIdentifier/url/http://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=10;spage=434;epage=442;aulast=Torresinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-05-06T16:51:06Zoai:ri.conicet.gov.ar:11336/114624instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-05-06 16:51:07.238CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bioguided isolation of antimicrobial polyphenols from Cuspidaria convoluta leaves and their synergistic effect with antibiotics
title Bioguided isolation of antimicrobial polyphenols from Cuspidaria convoluta leaves and their synergistic effect with antibiotics
spellingShingle Bioguided isolation of antimicrobial polyphenols from Cuspidaria convoluta leaves and their synergistic effect with antibiotics
Torres, Carola Analía
AMPICILLIN
ANTIMICROBIAL AGENTS
LUTEOLIN
MIC REDUCTION
title_short Bioguided isolation of antimicrobial polyphenols from Cuspidaria convoluta leaves and their synergistic effect with antibiotics
title_full Bioguided isolation of antimicrobial polyphenols from Cuspidaria convoluta leaves and their synergistic effect with antibiotics
title_fullStr Bioguided isolation of antimicrobial polyphenols from Cuspidaria convoluta leaves and their synergistic effect with antibiotics
title_full_unstemmed Bioguided isolation of antimicrobial polyphenols from Cuspidaria convoluta leaves and their synergistic effect with antibiotics
title_sort Bioguided isolation of antimicrobial polyphenols from Cuspidaria convoluta leaves and their synergistic effect with antibiotics
dc.creator.none.fl_str_mv Torres, Carola Analía
Sturla, Mario Aníbal
Romero, Ana María
Judis, María Alicia
author Torres, Carola Analía
author_facet Torres, Carola Analía
Sturla, Mario Aníbal
Romero, Ana María
Judis, María Alicia
author_role author
author2 Sturla, Mario Aníbal
Romero, Ana María
Judis, María Alicia
author2_role author
author
author
dc.subject.none.fl_str_mv AMPICILLIN
ANTIMICROBIAL AGENTS
LUTEOLIN
MIC REDUCTION
topic AMPICILLIN
ANTIMICROBIAL AGENTS
LUTEOLIN
MIC REDUCTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Objective: To identify and isolate phenolic compounds from Cuspidaria convoluta, and to evaluate their antibacterial activity and synergistic effect with antibiotics. Methods: The crude extract was prepared by maceration with methanol (5%). The dry extract was suspended in water and fractionated successively. The most active extract was selected by its antibacterial activity and its total phenol content was determined by spectrophotometry and by HPLC-MS/MS. Bioactive fractions of the most active extract were separated by column chromatography and evaluated by bioautography. Isolated compounds were identified. Minimum inhibitory concentration (MIC) of these compounds was determined by microdilution broth method, and synergism with antibiotics (ampicillin, gentamicin and oxacillin) was tested by checkerboard and time-kill assays. Results: Coumaric acid, catechin/epicatechin, and luteolin were purified and identified from the extract. There was an increase in the antibacterial activity of antibiotics when they were combined with these compounds. The combination of luteolin and ampicillin had the most potent antibacterial activities. The MICs of oxacillin for each of methicillin-resistant Staphylococcus aureus strains were reduced between 4 and 8-fold when these strains were coincubated with sub-MIC (≤ ½ MIC) levels of these compounds, demonstrating that the combination had synergistic effect for all cases. Conclusions: Cuspidaria convoluta contains important pharmacologically active substances that can be used to improve antibiotic efficacy.
Fil: Torres, Carola Analía. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Sturla, Mario Aníbal. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; Argentina
Fil: Romero, Ana María. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; Argentina
Fil: Judis, María Alicia. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; Argentina
description Objective: To identify and isolate phenolic compounds from Cuspidaria convoluta, and to evaluate their antibacterial activity and synergistic effect with antibiotics. Methods: The crude extract was prepared by maceration with methanol (5%). The dry extract was suspended in water and fractionated successively. The most active extract was selected by its antibacterial activity and its total phenol content was determined by spectrophotometry and by HPLC-MS/MS. Bioactive fractions of the most active extract were separated by column chromatography and evaluated by bioautography. Isolated compounds were identified. Minimum inhibitory concentration (MIC) of these compounds was determined by microdilution broth method, and synergism with antibiotics (ampicillin, gentamicin and oxacillin) was tested by checkerboard and time-kill assays. Results: Coumaric acid, catechin/epicatechin, and luteolin were purified and identified from the extract. There was an increase in the antibacterial activity of antibiotics when they were combined with these compounds. The combination of luteolin and ampicillin had the most potent antibacterial activities. The MICs of oxacillin for each of methicillin-resistant Staphylococcus aureus strains were reduced between 4 and 8-fold when these strains were coincubated with sub-MIC (≤ ½ MIC) levels of these compounds, demonstrating that the combination had synergistic effect for all cases. Conclusions: Cuspidaria convoluta contains important pharmacologically active substances that can be used to improve antibiotic efficacy.
publishDate 2019
dc.date.none.fl_str_mv 2019-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/114624
Torres, Carola Analía; Sturla, Mario Aníbal; Romero, Ana María; Judis, María Alicia; Bioguided isolation of antimicrobial polyphenols from Cuspidaria convoluta leaves and their synergistic effect with antibiotics; Wolters Kluwer Medknow Publications; Asian Pacific Journal of Tropical Biomedicine; 9; 10; 10-2019; 434-442
2221-1691
CONICET Digital
CONICET
url http://hdl.handle.net/11336/114624
identifier_str_mv Torres, Carola Analía; Sturla, Mario Aníbal; Romero, Ana María; Judis, María Alicia; Bioguided isolation of antimicrobial polyphenols from Cuspidaria convoluta leaves and their synergistic effect with antibiotics; Wolters Kluwer Medknow Publications; Asian Pacific Journal of Tropical Biomedicine; 9; 10; 10-2019; 434-442
2221-1691
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4103/2221-1691.269525
info:eu-repo/semantics/altIdentifier/url/http://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=10;spage=434;epage=442;aulast=Torres
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wolters Kluwer Medknow Publications
publisher.none.fl_str_mv Wolters Kluwer Medknow Publications
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.637756

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