Chitotetraose activates the fungal-dependent endosymbiotic signaling pathway in actinorhizal plant species
- Autores
- Chabaud, Mireille; Fournier, Joëlle; Brichet, Lukas; Abdou Pavy, Iltaf; Imanishi, Leandro Ezequiel; Brottier, Laurent; Pirolles, Elodie; Hocher, Valérie; Franche, Claudine; Bogusz, Didier; Wall, Luis Gabriel; Svistoonoff, Sergio; Gherbi, Hassen; Barker, David G.
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Mutualistic plant-microbe associations are widespread in natural ecosystems and have made major contributions throughout the evolutionary history of terrestrial plants. Amongst the most remarkable of these are the so-called root endosymbioses, resulting from the intracellular colonization of host tissues by either arbuscular mycorrhizal (AM) fungi or nitrogen-fixing bacteria that both provide key nutrients to the host in exchange for energy-rich photosynthates. Actinorhizal host plants, members of the Eurosid 1 clade, are able to associate with both AM fungi and nitrogen-fixing actinomycetes known as Frankia. Currently, little is known about the molecular signaling that allows these plants to recognize their fungal and bacterial partners. In this article, we describe the use of an in vivo Ca2+ reporter to identify symbiotic signaling responses to AM fungi in roots of both Casuarina glauca and Discaria trinervis, actinorhizal species with contrasting modes of Frankia colonization. This approach has revealed that, for both actinorhizal hosts, the short-chain chitin oligomer chitotetraose is able to mimic AM fungal exudates in activating the conserved symbiosis signaling pathway (CSSP) in epidermal root cells targeted by AM fungi. These results mirror findings in other AM host plants including legumes and the monocot rice. In addition, we show that chitotetraose is a more efficient elicitor of CSSP activation compared to AM fungal lipo-chitooligosaccharides. These findings reinforce the likely role of short-chain chitin oligomers during the initial stages of the AM association, and are discussed in relation to both our current knowledge about molecular signaling during Frankia recognition as well as the different microsymbiont root colonization mechanisms employed by actinorhizal hosts.
Fil: Chabaud, Mireille. University of Toulouse; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Fournier, Joëlle. University of Toulouse; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Brichet, Lukas. University of Toulouse; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Abdou Pavy, Iltaf. University of Toulouse; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Imanishi, Leandro Ezequiel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Brottier, Laurent. University of Montpellier; Francia. University of Montpellier SupAgro; Francia
Fil: Pirolles, Elodie. University of Montpellier SupAgro; Francia
Fil: Hocher, Valérie. University of Montpellier SupAgro; Francia
Fil: Franche, Claudine. University of Montpellier; Francia
Fil: Bogusz, Didier. University of Montpellier; Francia
Fil: Wall, Luis Gabriel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Svistoonoff, Sergio. University of Montpellier SupAgro; Francia
Fil: Gherbi, Hassen. University of Montpellier SupAgro; Francia
Fil: Barker, David G.. University of Toulouse; Francia. Centre National de la Recherche Scientifique; Francia - Materia
-
SYMBIOSIS
MICORRHIZA
FRANKIA
ACTINORHIZA - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/159502
Ver los metadatos del registro completo
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oai:ri.conicet.gov.ar:11336/159502 |
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Chitotetraose activates the fungal-dependent endosymbiotic signaling pathway in actinorhizal plant speciesChabaud, MireilleFournier, JoëlleBrichet, LukasAbdou Pavy, IltafImanishi, Leandro EzequielBrottier, LaurentPirolles, ElodieHocher, ValérieFranche, ClaudineBogusz, DidierWall, Luis GabrielSvistoonoff, SergioGherbi, HassenBarker, David G.SYMBIOSISMICORRHIZAFRANKIAACTINORHIZAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Mutualistic plant-microbe associations are widespread in natural ecosystems and have made major contributions throughout the evolutionary history of terrestrial plants. Amongst the most remarkable of these are the so-called root endosymbioses, resulting from the intracellular colonization of host tissues by either arbuscular mycorrhizal (AM) fungi or nitrogen-fixing bacteria that both provide key nutrients to the host in exchange for energy-rich photosynthates. Actinorhizal host plants, members of the Eurosid 1 clade, are able to associate with both AM fungi and nitrogen-fixing actinomycetes known as Frankia. Currently, little is known about the molecular signaling that allows these plants to recognize their fungal and bacterial partners. In this article, we describe the use of an in vivo Ca2+ reporter to identify symbiotic signaling responses to AM fungi in roots of both Casuarina glauca and Discaria trinervis, actinorhizal species with contrasting modes of Frankia colonization. This approach has revealed that, for both actinorhizal hosts, the short-chain chitin oligomer chitotetraose is able to mimic AM fungal exudates in activating the conserved symbiosis signaling pathway (CSSP) in epidermal root cells targeted by AM fungi. These results mirror findings in other AM host plants including legumes and the monocot rice. In addition, we show that chitotetraose is a more efficient elicitor of CSSP activation compared to AM fungal lipo-chitooligosaccharides. These findings reinforce the likely role of short-chain chitin oligomers during the initial stages of the AM association, and are discussed in relation to both our current knowledge about molecular signaling during Frankia recognition as well as the different microsymbiont root colonization mechanisms employed by actinorhizal hosts.Fil: Chabaud, Mireille. University of Toulouse; Francia. Centre National de la Recherche Scientifique; FranciaFil: Fournier, Joëlle. University of Toulouse; Francia. Centre National de la Recherche Scientifique; FranciaFil: Brichet, Lukas. University of Toulouse; Francia. Centre National de la Recherche Scientifique; FranciaFil: Abdou Pavy, Iltaf. University of Toulouse; Francia. Centre National de la Recherche Scientifique; FranciaFil: Imanishi, Leandro Ezequiel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Brottier, Laurent. University of Montpellier; Francia. University of Montpellier SupAgro; FranciaFil: Pirolles, Elodie. University of Montpellier SupAgro; FranciaFil: Hocher, Valérie. University of Montpellier SupAgro; FranciaFil: Franche, Claudine. University of Montpellier; FranciaFil: Bogusz, Didier. University of Montpellier; FranciaFil: Wall, Luis Gabriel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Svistoonoff, Sergio. University of Montpellier SupAgro; FranciaFil: Gherbi, Hassen. University of Montpellier SupAgro; FranciaFil: Barker, David G.. University of Toulouse; Francia. Centre National de la Recherche Scientifique; FranciaPublic Library of Science2019-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/159502Chabaud, Mireille; Fournier, Joëlle; Brichet, Lukas; Abdou Pavy, Iltaf; Imanishi, Leandro Ezequiel; et al.; Chitotetraose activates the fungal-dependent endosymbiotic signaling pathway in actinorhizal plant species; Public Library of Science; Plos One; 14; 10; 10-2019; 1-13; e02231491932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0223149info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0223149info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:59:43Zoai:ri.conicet.gov.ar:11336/159502instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:59:43.499CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Chitotetraose activates the fungal-dependent endosymbiotic signaling pathway in actinorhizal plant species |
title |
Chitotetraose activates the fungal-dependent endosymbiotic signaling pathway in actinorhizal plant species |
spellingShingle |
Chitotetraose activates the fungal-dependent endosymbiotic signaling pathway in actinorhizal plant species Chabaud, Mireille SYMBIOSIS MICORRHIZA FRANKIA ACTINORHIZA |
title_short |
Chitotetraose activates the fungal-dependent endosymbiotic signaling pathway in actinorhizal plant species |
title_full |
Chitotetraose activates the fungal-dependent endosymbiotic signaling pathway in actinorhizal plant species |
title_fullStr |
Chitotetraose activates the fungal-dependent endosymbiotic signaling pathway in actinorhizal plant species |
title_full_unstemmed |
Chitotetraose activates the fungal-dependent endosymbiotic signaling pathway in actinorhizal plant species |
title_sort |
Chitotetraose activates the fungal-dependent endosymbiotic signaling pathway in actinorhizal plant species |
dc.creator.none.fl_str_mv |
Chabaud, Mireille Fournier, Joëlle Brichet, Lukas Abdou Pavy, Iltaf Imanishi, Leandro Ezequiel Brottier, Laurent Pirolles, Elodie Hocher, Valérie Franche, Claudine Bogusz, Didier Wall, Luis Gabriel Svistoonoff, Sergio Gherbi, Hassen Barker, David G. |
author |
Chabaud, Mireille |
author_facet |
Chabaud, Mireille Fournier, Joëlle Brichet, Lukas Abdou Pavy, Iltaf Imanishi, Leandro Ezequiel Brottier, Laurent Pirolles, Elodie Hocher, Valérie Franche, Claudine Bogusz, Didier Wall, Luis Gabriel Svistoonoff, Sergio Gherbi, Hassen Barker, David G. |
author_role |
author |
author2 |
Fournier, Joëlle Brichet, Lukas Abdou Pavy, Iltaf Imanishi, Leandro Ezequiel Brottier, Laurent Pirolles, Elodie Hocher, Valérie Franche, Claudine Bogusz, Didier Wall, Luis Gabriel Svistoonoff, Sergio Gherbi, Hassen Barker, David G. |
author2_role |
author author author author author author author author author author author author author |
dc.subject.none.fl_str_mv |
SYMBIOSIS MICORRHIZA FRANKIA ACTINORHIZA |
topic |
SYMBIOSIS MICORRHIZA FRANKIA ACTINORHIZA |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Mutualistic plant-microbe associations are widespread in natural ecosystems and have made major contributions throughout the evolutionary history of terrestrial plants. Amongst the most remarkable of these are the so-called root endosymbioses, resulting from the intracellular colonization of host tissues by either arbuscular mycorrhizal (AM) fungi or nitrogen-fixing bacteria that both provide key nutrients to the host in exchange for energy-rich photosynthates. Actinorhizal host plants, members of the Eurosid 1 clade, are able to associate with both AM fungi and nitrogen-fixing actinomycetes known as Frankia. Currently, little is known about the molecular signaling that allows these plants to recognize their fungal and bacterial partners. In this article, we describe the use of an in vivo Ca2+ reporter to identify symbiotic signaling responses to AM fungi in roots of both Casuarina glauca and Discaria trinervis, actinorhizal species with contrasting modes of Frankia colonization. This approach has revealed that, for both actinorhizal hosts, the short-chain chitin oligomer chitotetraose is able to mimic AM fungal exudates in activating the conserved symbiosis signaling pathway (CSSP) in epidermal root cells targeted by AM fungi. These results mirror findings in other AM host plants including legumes and the monocot rice. In addition, we show that chitotetraose is a more efficient elicitor of CSSP activation compared to AM fungal lipo-chitooligosaccharides. These findings reinforce the likely role of short-chain chitin oligomers during the initial stages of the AM association, and are discussed in relation to both our current knowledge about molecular signaling during Frankia recognition as well as the different microsymbiont root colonization mechanisms employed by actinorhizal hosts. Fil: Chabaud, Mireille. University of Toulouse; Francia. Centre National de la Recherche Scientifique; Francia Fil: Fournier, Joëlle. University of Toulouse; Francia. Centre National de la Recherche Scientifique; Francia Fil: Brichet, Lukas. University of Toulouse; Francia. Centre National de la Recherche Scientifique; Francia Fil: Abdou Pavy, Iltaf. University of Toulouse; Francia. Centre National de la Recherche Scientifique; Francia Fil: Imanishi, Leandro Ezequiel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Brottier, Laurent. University of Montpellier; Francia. University of Montpellier SupAgro; Francia Fil: Pirolles, Elodie. University of Montpellier SupAgro; Francia Fil: Hocher, Valérie. University of Montpellier SupAgro; Francia Fil: Franche, Claudine. University of Montpellier; Francia Fil: Bogusz, Didier. University of Montpellier; Francia Fil: Wall, Luis Gabriel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Svistoonoff, Sergio. University of Montpellier SupAgro; Francia Fil: Gherbi, Hassen. University of Montpellier SupAgro; Francia Fil: Barker, David G.. University of Toulouse; Francia. Centre National de la Recherche Scientifique; Francia |
description |
Mutualistic plant-microbe associations are widespread in natural ecosystems and have made major contributions throughout the evolutionary history of terrestrial plants. Amongst the most remarkable of these are the so-called root endosymbioses, resulting from the intracellular colonization of host tissues by either arbuscular mycorrhizal (AM) fungi or nitrogen-fixing bacteria that both provide key nutrients to the host in exchange for energy-rich photosynthates. Actinorhizal host plants, members of the Eurosid 1 clade, are able to associate with both AM fungi and nitrogen-fixing actinomycetes known as Frankia. Currently, little is known about the molecular signaling that allows these plants to recognize their fungal and bacterial partners. In this article, we describe the use of an in vivo Ca2+ reporter to identify symbiotic signaling responses to AM fungi in roots of both Casuarina glauca and Discaria trinervis, actinorhizal species with contrasting modes of Frankia colonization. This approach has revealed that, for both actinorhizal hosts, the short-chain chitin oligomer chitotetraose is able to mimic AM fungal exudates in activating the conserved symbiosis signaling pathway (CSSP) in epidermal root cells targeted by AM fungi. These results mirror findings in other AM host plants including legumes and the monocot rice. In addition, we show that chitotetraose is a more efficient elicitor of CSSP activation compared to AM fungal lipo-chitooligosaccharides. These findings reinforce the likely role of short-chain chitin oligomers during the initial stages of the AM association, and are discussed in relation to both our current knowledge about molecular signaling during Frankia recognition as well as the different microsymbiont root colonization mechanisms employed by actinorhizal hosts. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/159502 Chabaud, Mireille; Fournier, Joëlle; Brichet, Lukas; Abdou Pavy, Iltaf; Imanishi, Leandro Ezequiel; et al.; Chitotetraose activates the fungal-dependent endosymbiotic signaling pathway in actinorhizal plant species; Public Library of Science; Plos One; 14; 10; 10-2019; 1-13; e0223149 1932-6203 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/159502 |
identifier_str_mv |
Chabaud, Mireille; Fournier, Joëlle; Brichet, Lukas; Abdou Pavy, Iltaf; Imanishi, Leandro Ezequiel; et al.; Chitotetraose activates the fungal-dependent endosymbiotic signaling pathway in actinorhizal plant species; Public Library of Science; Plos One; 14; 10; 10-2019; 1-13; e0223149 1932-6203 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0223149 info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0223149 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Public Library of Science |
publisher.none.fl_str_mv |
Public Library of Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613770067836928 |
score |
13.070432 |