PLS regression in the spectrophotometric data for the simultaneous determination of levodopa and carbidopa in pharmaceutical preparations by using an enzymatic stopped-flow FIA tec...

Autores
Grunhut, Marcos; Centurión, María Eugenia; Fernández Band, Beatriz Susana
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
An enzymatic stopped-flow-injection analysis is proposed for simultaneous determination of levodopa and carbidopa in pharmaceutical preparations. The dopaquinones obtained after the oxidation catalized by the enzyme were measured by spectrophotometric method. A reduced calibration matrix based on a central composite experimental design was built and Partial Least Squares (PLS) was applied on the spectral data after reaction with the enzyme. The LOD was 0.015 and 0.0028 mg ml-1, respectively and the sample throughput was 22.5 h-1. The proposed method was applied to pharmaceutical preparations and the results are in close agreement with pharmacopeial method. The recovery study and results were satisfactory.
Fil: Grunhut, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Centurión, María Eugenia. Universidad Nacional del Sur. Departamento de Química; Argentina
Fil: Fernández Band, Beatriz Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Materia
CARBIDOPA
LEVODOPA
PLS
POLYPHENOL OXIDASE
STOPPED-FLOW FIA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/95338
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/95338
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling PLS regression in the spectrophotometric data for the simultaneous determination of levodopa and carbidopa in pharmaceutical preparations by using an enzymatic stopped-flow FIA techniqueGrunhut, MarcosCenturión, María EugeniaFernández Band, Beatriz SusanaCARBIDOPALEVODOPAPLSPOLYPHENOL OXIDASESTOPPED-FLOW FIAhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1An enzymatic stopped-flow-injection analysis is proposed for simultaneous determination of levodopa and carbidopa in pharmaceutical preparations. The dopaquinones obtained after the oxidation catalized by the enzyme were measured by spectrophotometric method. A reduced calibration matrix based on a central composite experimental design was built and Partial Least Squares (PLS) was applied on the spectral data after reaction with the enzyme. The LOD was 0.015 and 0.0028 mg ml-1, respectively and the sample throughput was 22.5 h-1. The proposed method was applied to pharmaceutical preparations and the results are in close agreement with pharmacopeial method. The recovery study and results were satisfactory.Fil: Grunhut, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Centurión, María Eugenia. Universidad Nacional del Sur. Departamento de Química; ArgentinaFil: Fernández Band, Beatriz Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaTaylor & Francis2007-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/95338Grunhut, Marcos; Centurión, María Eugenia; Fernández Band, Beatriz Susana; PLS regression in the spectrophotometric data for the simultaneous determination of levodopa and carbidopa in pharmaceutical preparations by using an enzymatic stopped-flow FIA technique; Taylor & Francis; Analytical Letters; 40; 10; 1-2007; 2016-20310003-2719CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1080/00032710701486249info:eu-repo/semantics/altIdentifier/doi/10.1080/00032710701486249info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:09:37Zoai:ri.conicet.gov.ar:11336/95338instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:09:38.157CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv PLS regression in the spectrophotometric data for the simultaneous determination of levodopa and carbidopa in pharmaceutical preparations by using an enzymatic stopped-flow FIA technique
title PLS regression in the spectrophotometric data for the simultaneous determination of levodopa and carbidopa in pharmaceutical preparations by using an enzymatic stopped-flow FIA technique
spellingShingle PLS regression in the spectrophotometric data for the simultaneous determination of levodopa and carbidopa in pharmaceutical preparations by using an enzymatic stopped-flow FIA technique
Grunhut, Marcos
CARBIDOPA
LEVODOPA
PLS
POLYPHENOL OXIDASE
STOPPED-FLOW FIA
title_short PLS regression in the spectrophotometric data for the simultaneous determination of levodopa and carbidopa in pharmaceutical preparations by using an enzymatic stopped-flow FIA technique
title_full PLS regression in the spectrophotometric data for the simultaneous determination of levodopa and carbidopa in pharmaceutical preparations by using an enzymatic stopped-flow FIA technique
title_fullStr PLS regression in the spectrophotometric data for the simultaneous determination of levodopa and carbidopa in pharmaceutical preparations by using an enzymatic stopped-flow FIA technique
title_full_unstemmed PLS regression in the spectrophotometric data for the simultaneous determination of levodopa and carbidopa in pharmaceutical preparations by using an enzymatic stopped-flow FIA technique
title_sort PLS regression in the spectrophotometric data for the simultaneous determination of levodopa and carbidopa in pharmaceutical preparations by using an enzymatic stopped-flow FIA technique
dc.creator.none.fl_str_mv Grunhut, Marcos
Centurión, María Eugenia
Fernández Band, Beatriz Susana
author Grunhut, Marcos
author_facet Grunhut, Marcos
Centurión, María Eugenia
Fernández Band, Beatriz Susana
author_role author
author2 Centurión, María Eugenia
Fernández Band, Beatriz Susana
author2_role author
author
dc.subject.none.fl_str_mv CARBIDOPA
LEVODOPA
PLS
POLYPHENOL OXIDASE
STOPPED-FLOW FIA
topic CARBIDOPA
LEVODOPA
PLS
POLYPHENOL OXIDASE
STOPPED-FLOW FIA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv An enzymatic stopped-flow-injection analysis is proposed for simultaneous determination of levodopa and carbidopa in pharmaceutical preparations. The dopaquinones obtained after the oxidation catalized by the enzyme were measured by spectrophotometric method. A reduced calibration matrix based on a central composite experimental design was built and Partial Least Squares (PLS) was applied on the spectral data after reaction with the enzyme. The LOD was 0.015 and 0.0028 mg ml-1, respectively and the sample throughput was 22.5 h-1. The proposed method was applied to pharmaceutical preparations and the results are in close agreement with pharmacopeial method. The recovery study and results were satisfactory.
Fil: Grunhut, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Centurión, María Eugenia. Universidad Nacional del Sur. Departamento de Química; Argentina
Fil: Fernández Band, Beatriz Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
description An enzymatic stopped-flow-injection analysis is proposed for simultaneous determination of levodopa and carbidopa in pharmaceutical preparations. The dopaquinones obtained after the oxidation catalized by the enzyme were measured by spectrophotometric method. A reduced calibration matrix based on a central composite experimental design was built and Partial Least Squares (PLS) was applied on the spectral data after reaction with the enzyme. The LOD was 0.015 and 0.0028 mg ml-1, respectively and the sample throughput was 22.5 h-1. The proposed method was applied to pharmaceutical preparations and the results are in close agreement with pharmacopeial method. The recovery study and results were satisfactory.
publishDate 2007
dc.date.none.fl_str_mv 2007-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/95338
Grunhut, Marcos; Centurión, María Eugenia; Fernández Band, Beatriz Susana; PLS regression in the spectrophotometric data for the simultaneous determination of levodopa and carbidopa in pharmaceutical preparations by using an enzymatic stopped-flow FIA technique; Taylor & Francis; Analytical Letters; 40; 10; 1-2007; 2016-2031
0003-2719
CONICET Digital
CONICET
url http://hdl.handle.net/11336/95338
identifier_str_mv Grunhut, Marcos; Centurión, María Eugenia; Fernández Band, Beatriz Susana; PLS regression in the spectrophotometric data for the simultaneous determination of levodopa and carbidopa in pharmaceutical preparations by using an enzymatic stopped-flow FIA technique; Taylor & Francis; Analytical Letters; 40; 10; 1-2007; 2016-2031
0003-2719
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1080/00032710701486249
info:eu-repo/semantics/altIdentifier/doi/10.1080/00032710701486249
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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