Multivariate analysis for resolving interactions of carbidopa with dsDNA at a fullerene-C60/GCE

Autores
Gholivand, Mohammad Bagher; Jalalvand, Ali R.; Goicoechea, Hector Casimiro
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
For the first time, interactions of carbidopa (CD) with double-stranded calf thymus DNA (dsDNA) in a phosphate buffered solution (PBS, 0.05 M, pH = 4.0) at a fullerene-C60/glassy carbon electrode (FLR/GCE) has been studied by cyclic voltammetry (CV), linear sweep voltammetry (LSV), and square wave voltammetry (SWV). The interaction of CD with dsDNA was also monitored using fluorescence (F) and UVvis spectroscopic techniques. New information was obtained when a row- and column-wise augmented matrix consisting of SWV, LSV, F and UVvis data was resolved using multivariate curve resolution-alternating least squares (MCR-ALS) as a powerful chemometric tool. Pure electrochemical and spectroscopic signals of CD, dsDNA and dsDNA-CD2 complex, and their concentration profiles were then successfully resolved. Molecular docking studies confirmed that the binding of CD with dsDNA shows minor groove binding mode which was in accordance with experimental results. Under optimized conditions, the SWV responses were linearly related to dsDNA concentration between 0.1 and 25.0 nM and a limit of detection (LOD) of 0.03 nM was calculated (3Sb/b = 3). Moreover, the modified electrode exhibited long term stability, good repeatability, and reproducibility, and high sensitivity and selectivity towards dsDNA determination in human serum samples, demonstrating its feasibility towards dsDNA sensing.
Fil: Gholivand, Mohammad Bagher. Razi University. Faculty of Chemistry; Irán
Fil: Jalalvand, Ali R.. Razi University. Faculty of Chemistry; Irán
Fil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral. Cátedra de Química Analítica I. Laboratorio de Desarrollo Analítico y Quimiometría; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe; Argentina
Materia
Carbidopa
Dsdna
Voltametry
Spectroscopy
Fullerene C60
Chemometrics
Molecular Docking
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/15364
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/15364
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Multivariate analysis for resolving interactions of carbidopa with dsDNA at a fullerene-C60/GCEGholivand, Mohammad BagherJalalvand, Ali R.Goicoechea, Hector CasimiroCarbidopaDsdnaVoltametrySpectroscopyFullerene C60ChemometricsMolecular Dockinghttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1For the first time, interactions of carbidopa (CD) with double-stranded calf thymus DNA (dsDNA) in a phosphate buffered solution (PBS, 0.05 M, pH = 4.0) at a fullerene-C60/glassy carbon electrode (FLR/GCE) has been studied by cyclic voltammetry (CV), linear sweep voltammetry (LSV), and square wave voltammetry (SWV). The interaction of CD with dsDNA was also monitored using fluorescence (F) and UVvis spectroscopic techniques. New information was obtained when a row- and column-wise augmented matrix consisting of SWV, LSV, F and UVvis data was resolved using multivariate curve resolution-alternating least squares (MCR-ALS) as a powerful chemometric tool. Pure electrochemical and spectroscopic signals of CD, dsDNA and dsDNA-CD2 complex, and their concentration profiles were then successfully resolved. Molecular docking studies confirmed that the binding of CD with dsDNA shows minor groove binding mode which was in accordance with experimental results. Under optimized conditions, the SWV responses were linearly related to dsDNA concentration between 0.1 and 25.0 nM and a limit of detection (LOD) of 0.03 nM was calculated (3Sb/b = 3). Moreover, the modified electrode exhibited long term stability, good repeatability, and reproducibility, and high sensitivity and selectivity towards dsDNA determination in human serum samples, demonstrating its feasibility towards dsDNA sensing.Fil: Gholivand, Mohammad Bagher. Razi University. Faculty of Chemistry; IránFil: Jalalvand, Ali R.. Razi University. Faculty of Chemistry; IránFil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral. Cátedra de Química Analítica I. Laboratorio de Desarrollo Analítico y Quimiometría; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe; ArgentinaElsevier Science2014-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/15364Gholivand, Mohammad Bagher; Jalalvand, Ali R.; Goicoechea, Hector Casimiro; Multivariate analysis for resolving interactions of carbidopa with dsDNA at a fullerene-C60/GCE; Elsevier Science; International Journal Of Biological Macromolecules; 69; 6-2014; 369-3810141-8130enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijbiomac.2014.05.074info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S014181301400381Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:37:09Zoai:ri.conicet.gov.ar:11336/15364instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:37:09.548CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Multivariate analysis for resolving interactions of carbidopa with dsDNA at a fullerene-C60/GCE
title Multivariate analysis for resolving interactions of carbidopa with dsDNA at a fullerene-C60/GCE
spellingShingle Multivariate analysis for resolving interactions of carbidopa with dsDNA at a fullerene-C60/GCE
Gholivand, Mohammad Bagher
Carbidopa
Dsdna
Voltametry
Spectroscopy
Fullerene C60
Chemometrics
Molecular Docking
title_short Multivariate analysis for resolving interactions of carbidopa with dsDNA at a fullerene-C60/GCE
title_full Multivariate analysis for resolving interactions of carbidopa with dsDNA at a fullerene-C60/GCE
title_fullStr Multivariate analysis for resolving interactions of carbidopa with dsDNA at a fullerene-C60/GCE
title_full_unstemmed Multivariate analysis for resolving interactions of carbidopa with dsDNA at a fullerene-C60/GCE
title_sort Multivariate analysis for resolving interactions of carbidopa with dsDNA at a fullerene-C60/GCE
dc.creator.none.fl_str_mv Gholivand, Mohammad Bagher
Jalalvand, Ali R.
Goicoechea, Hector Casimiro
author Gholivand, Mohammad Bagher
author_facet Gholivand, Mohammad Bagher
Jalalvand, Ali R.
Goicoechea, Hector Casimiro
author_role author
author2 Jalalvand, Ali R.
Goicoechea, Hector Casimiro
author2_role author
author
dc.subject.none.fl_str_mv Carbidopa
Dsdna
Voltametry
Spectroscopy
Fullerene C60
Chemometrics
Molecular Docking
topic Carbidopa
Dsdna
Voltametry
Spectroscopy
Fullerene C60
Chemometrics
Molecular Docking
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv For the first time, interactions of carbidopa (CD) with double-stranded calf thymus DNA (dsDNA) in a phosphate buffered solution (PBS, 0.05 M, pH = 4.0) at a fullerene-C60/glassy carbon electrode (FLR/GCE) has been studied by cyclic voltammetry (CV), linear sweep voltammetry (LSV), and square wave voltammetry (SWV). The interaction of CD with dsDNA was also monitored using fluorescence (F) and UVvis spectroscopic techniques. New information was obtained when a row- and column-wise augmented matrix consisting of SWV, LSV, F and UVvis data was resolved using multivariate curve resolution-alternating least squares (MCR-ALS) as a powerful chemometric tool. Pure electrochemical and spectroscopic signals of CD, dsDNA and dsDNA-CD2 complex, and their concentration profiles were then successfully resolved. Molecular docking studies confirmed that the binding of CD with dsDNA shows minor groove binding mode which was in accordance with experimental results. Under optimized conditions, the SWV responses were linearly related to dsDNA concentration between 0.1 and 25.0 nM and a limit of detection (LOD) of 0.03 nM was calculated (3Sb/b = 3). Moreover, the modified electrode exhibited long term stability, good repeatability, and reproducibility, and high sensitivity and selectivity towards dsDNA determination in human serum samples, demonstrating its feasibility towards dsDNA sensing.
Fil: Gholivand, Mohammad Bagher. Razi University. Faculty of Chemistry; Irán
Fil: Jalalvand, Ali R.. Razi University. Faculty of Chemistry; Irán
Fil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral. Cátedra de Química Analítica I. Laboratorio de Desarrollo Analítico y Quimiometría; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe; Argentina
description For the first time, interactions of carbidopa (CD) with double-stranded calf thymus DNA (dsDNA) in a phosphate buffered solution (PBS, 0.05 M, pH = 4.0) at a fullerene-C60/glassy carbon electrode (FLR/GCE) has been studied by cyclic voltammetry (CV), linear sweep voltammetry (LSV), and square wave voltammetry (SWV). The interaction of CD with dsDNA was also monitored using fluorescence (F) and UVvis spectroscopic techniques. New information was obtained when a row- and column-wise augmented matrix consisting of SWV, LSV, F and UVvis data was resolved using multivariate curve resolution-alternating least squares (MCR-ALS) as a powerful chemometric tool. Pure electrochemical and spectroscopic signals of CD, dsDNA and dsDNA-CD2 complex, and their concentration profiles were then successfully resolved. Molecular docking studies confirmed that the binding of CD with dsDNA shows minor groove binding mode which was in accordance with experimental results. Under optimized conditions, the SWV responses were linearly related to dsDNA concentration between 0.1 and 25.0 nM and a limit of detection (LOD) of 0.03 nM was calculated (3Sb/b = 3). Moreover, the modified electrode exhibited long term stability, good repeatability, and reproducibility, and high sensitivity and selectivity towards dsDNA determination in human serum samples, demonstrating its feasibility towards dsDNA sensing.
publishDate 2014
dc.date.none.fl_str_mv 2014-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/15364
Gholivand, Mohammad Bagher; Jalalvand, Ali R.; Goicoechea, Hector Casimiro; Multivariate analysis for resolving interactions of carbidopa with dsDNA at a fullerene-C60/GCE; Elsevier Science; International Journal Of Biological Macromolecules; 69; 6-2014; 369-381
0141-8130
url http://hdl.handle.net/11336/15364
identifier_str_mv Gholivand, Mohammad Bagher; Jalalvand, Ali R.; Goicoechea, Hector Casimiro; Multivariate analysis for resolving interactions of carbidopa with dsDNA at a fullerene-C60/GCE; Elsevier Science; International Journal Of Biological Macromolecules; 69; 6-2014; 369-381
0141-8130
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijbiomac.2014.05.074
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S014181301400381X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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