In Situ Pressure Probe Sampling and UV-MALDI MS for Profiling Metabolites in Living Single Cells

Autores
Yousef Gholipour; Erra Balsells, Rosa; Nonami, Hiroshi
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this work we describe the use of a combination of a cell pressure probe and a UV-matrix-assisted laser desorption/ ionization time of flight (UV-MALDI-TOF) mass spectrometer for the in situ picoliter sampling and shotgun metabolite profiling of living single cells of plants. In addition to quantifiable sampling, the pressure probe has some unique features which differentiate it from other single-cell analytical tools. Cell wall and plasma membrane properties and water relations of in situ living single cells can be analyzed before sampling the cell sap. In addition, the fully-controlled sampling of cells located at different depths in plant tissues, measurement of the sample volume, and the addition of internal standards are facilitated by the pressure probe. Using a variety of organic compounds and nanoparticles as UV-MALDI matrices, metabolites from neutral carbohydrates to amino acids and other metabolites can be detected through UV-MALDI-TOF mass spectrometry analyses of picoliter-sized, single-cell samples.
Fil: Yousef Gholipour. Ehime University; Japón
Fil: Erra Balsells, Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina. Ehime University; Japón
Fil: Nonami, Hiroshi. Ehime University; Japón
Materia
Pressure Probe
Maldi-Ms
Metabolimcs
Cell Analysis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/68277
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In Situ Pressure Probe Sampling and UV-MALDI MS for Profiling Metabolites in Living Single CellsYousef GholipourErra Balsells, RosaNonami, HiroshiPressure ProbeMaldi-MsMetabolimcsCell Analysishttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1In this work we describe the use of a combination of a cell pressure probe and a UV-matrix-assisted laser desorption/ ionization time of flight (UV-MALDI-TOF) mass spectrometer for the in situ picoliter sampling and shotgun metabolite profiling of living single cells of plants. In addition to quantifiable sampling, the pressure probe has some unique features which differentiate it from other single-cell analytical tools. Cell wall and plasma membrane properties and water relations of in situ living single cells can be analyzed before sampling the cell sap. In addition, the fully-controlled sampling of cells located at different depths in plant tissues, measurement of the sample volume, and the addition of internal standards are facilitated by the pressure probe. Using a variety of organic compounds and nanoparticles as UV-MALDI matrices, metabolites from neutral carbohydrates to amino acids and other metabolites can be detected through UV-MALDI-TOF mass spectrometry analyses of picoliter-sized, single-cell samples.Fil: Yousef Gholipour. Ehime University; JapónFil: Erra Balsells, Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina. Ehime University; JapónFil: Nonami, Hiroshi. Ehime University; JapónMass Spectrometry Society of Japan2012-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/68277Yousef Gholipour; Erra Balsells, Rosa; Nonami, Hiroshi; In Situ Pressure Probe Sampling and UV-MALDI MS for Profiling Metabolites in Living Single Cells; Mass Spectrometry Society of Japan; Mass Spectrometry; 1; 1; 8-2012; 1-112187-137X2186-5116CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.5702/massspectrometry.A0003info:eu-repo/semantics/altIdentifier/url/https://www.jstage.jst.go.jp/article/massspectrometry/1/1/1_A0003/_articleinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:43:39Zoai:ri.conicet.gov.ar:11336/68277instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:43:39.654CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In Situ Pressure Probe Sampling and UV-MALDI MS for Profiling Metabolites in Living Single Cells
title In Situ Pressure Probe Sampling and UV-MALDI MS for Profiling Metabolites in Living Single Cells
spellingShingle In Situ Pressure Probe Sampling and UV-MALDI MS for Profiling Metabolites in Living Single Cells
Yousef Gholipour
Pressure Probe
Maldi-Ms
Metabolimcs
Cell Analysis
title_short In Situ Pressure Probe Sampling and UV-MALDI MS for Profiling Metabolites in Living Single Cells
title_full In Situ Pressure Probe Sampling and UV-MALDI MS for Profiling Metabolites in Living Single Cells
title_fullStr In Situ Pressure Probe Sampling and UV-MALDI MS for Profiling Metabolites in Living Single Cells
title_full_unstemmed In Situ Pressure Probe Sampling and UV-MALDI MS for Profiling Metabolites in Living Single Cells
title_sort In Situ Pressure Probe Sampling and UV-MALDI MS for Profiling Metabolites in Living Single Cells
dc.creator.none.fl_str_mv Yousef Gholipour
Erra Balsells, Rosa
Nonami, Hiroshi
author Yousef Gholipour
author_facet Yousef Gholipour
Erra Balsells, Rosa
Nonami, Hiroshi
author_role author
author2 Erra Balsells, Rosa
Nonami, Hiroshi
author2_role author
author
dc.subject.none.fl_str_mv Pressure Probe
Maldi-Ms
Metabolimcs
Cell Analysis
topic Pressure Probe
Maldi-Ms
Metabolimcs
Cell Analysis
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In this work we describe the use of a combination of a cell pressure probe and a UV-matrix-assisted laser desorption/ ionization time of flight (UV-MALDI-TOF) mass spectrometer for the in situ picoliter sampling and shotgun metabolite profiling of living single cells of plants. In addition to quantifiable sampling, the pressure probe has some unique features which differentiate it from other single-cell analytical tools. Cell wall and plasma membrane properties and water relations of in situ living single cells can be analyzed before sampling the cell sap. In addition, the fully-controlled sampling of cells located at different depths in plant tissues, measurement of the sample volume, and the addition of internal standards are facilitated by the pressure probe. Using a variety of organic compounds and nanoparticles as UV-MALDI matrices, metabolites from neutral carbohydrates to amino acids and other metabolites can be detected through UV-MALDI-TOF mass spectrometry analyses of picoliter-sized, single-cell samples.
Fil: Yousef Gholipour. Ehime University; Japón
Fil: Erra Balsells, Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina. Ehime University; Japón
Fil: Nonami, Hiroshi. Ehime University; Japón
description In this work we describe the use of a combination of a cell pressure probe and a UV-matrix-assisted laser desorption/ ionization time of flight (UV-MALDI-TOF) mass spectrometer for the in situ picoliter sampling and shotgun metabolite profiling of living single cells of plants. In addition to quantifiable sampling, the pressure probe has some unique features which differentiate it from other single-cell analytical tools. Cell wall and plasma membrane properties and water relations of in situ living single cells can be analyzed before sampling the cell sap. In addition, the fully-controlled sampling of cells located at different depths in plant tissues, measurement of the sample volume, and the addition of internal standards are facilitated by the pressure probe. Using a variety of organic compounds and nanoparticles as UV-MALDI matrices, metabolites from neutral carbohydrates to amino acids and other metabolites can be detected through UV-MALDI-TOF mass spectrometry analyses of picoliter-sized, single-cell samples.
publishDate 2012
dc.date.none.fl_str_mv 2012-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/68277
Yousef Gholipour; Erra Balsells, Rosa; Nonami, Hiroshi; In Situ Pressure Probe Sampling and UV-MALDI MS for Profiling Metabolites in Living Single Cells; Mass Spectrometry Society of Japan; Mass Spectrometry; 1; 1; 8-2012; 1-11
2187-137X
2186-5116
CONICET Digital
CONICET
url http://hdl.handle.net/11336/68277
identifier_str_mv Yousef Gholipour; Erra Balsells, Rosa; Nonami, Hiroshi; In Situ Pressure Probe Sampling and UV-MALDI MS for Profiling Metabolites in Living Single Cells; Mass Spectrometry Society of Japan; Mass Spectrometry; 1; 1; 8-2012; 1-11
2187-137X
2186-5116
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.5702/massspectrometry.A0003
info:eu-repo/semantics/altIdentifier/url/https://www.jstage.jst.go.jp/article/massspectrometry/1/1/1_A0003/_article
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Mass Spectrometry Society of Japan
publisher.none.fl_str_mv Mass Spectrometry Society of Japan
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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